Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS09890 and RPSI07_RS09895 are separated by 119 nucleotides RPSI07_RS09895 and RPSI07_RS09900 are separated by 490 nucleotides
RPSI07_RS09890: RPSI07_RS09890 - DeoR/GlpR transcriptional regulator, at 438,285 to 439,049
_RS09890
RPSI07_RS09895: RPSI07_RS09895 - CHAD domain-containing protein, at 439,169 to 440,026
_RS09895
RPSI07_RS09900: RPSI07_RS09900 - 16S ribosomal RNA, at 440,517 to 442,063
_RS09900
Position (kb) 439
440
441 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 438.207 kb on + strand at 438.212 kb on + strand at 438.212 kb on + strand at 438.212 kb on + strand at 438.212 kb on + strand at 438.213 kb on - strand at 438.239 kb on - strand at 438.263 kb on + strand at 438.263 kb on + strand at 438.264 kb on - strand at 438.264 kb on - strand at 438.316 kb on - strand at 438.477 kb on + strand, within RPSI07_RS09890 at 438.478 kb on - strand, within RPSI07_RS09890 at 438.478 kb on - strand, within RPSI07_RS09890 at 438.498 kb on + strand, within RPSI07_RS09890 at 438.498 kb on + strand, within RPSI07_RS09890 at 438.498 kb on + strand, within RPSI07_RS09890 at 438.499 kb on - strand, within RPSI07_RS09890 at 438.499 kb on - strand, within RPSI07_RS09890 at 438.499 kb on - strand, within RPSI07_RS09890 at 438.499 kb on - strand, within RPSI07_RS09890 at 438.499 kb on - strand, within RPSI07_RS09890 at 438.499 kb on - strand, within RPSI07_RS09890 at 438.828 kb on + strand, within RPSI07_RS09890 at 438.829 kb on - strand, within RPSI07_RS09890 at 438.829 kb on - strand, within RPSI07_RS09890 at 438.834 kb on + strand, within RPSI07_RS09890 at 439.076 kb on - strand at 439.076 kb on - strand at 439.076 kb on - strand at 439.076 kb on - strand at 439.362 kb on + strand, within RPSI07_RS09895 at 439.362 kb on + strand, within RPSI07_RS09895 at 439.362 kb on + strand, within RPSI07_RS09895 at 439.362 kb on + strand, within RPSI07_RS09895 at 439.362 kb on + strand, within RPSI07_RS09895 at 439.362 kb on + strand, within RPSI07_RS09895 at 439.363 kb on - strand, within RPSI07_RS09895 at 439.363 kb on - strand, within RPSI07_RS09895 at 439.363 kb on - strand, within RPSI07_RS09895 at 439.363 kb on - strand, within RPSI07_RS09895 at 439.363 kb on - strand, within RPSI07_RS09895 at 439.486 kb on + strand, within RPSI07_RS09895 at 439.486 kb on + strand, within RPSI07_RS09895 at 439.487 kb on - strand, within RPSI07_RS09895 at 439.487 kb on - strand, within RPSI07_RS09895 at 439.689 kb on + strand, within RPSI07_RS09895 at 439.690 kb on - strand, within RPSI07_RS09895 at 439.782 kb on - strand, within RPSI07_RS09895 at 439.787 kb on + strand, within RPSI07_RS09895 at 439.788 kb on - strand, within RPSI07_RS09895 at 439.788 kb on - strand, within RPSI07_RS09895 at 439.788 kb on - strand, within RPSI07_RS09895 at 439.950 kb on + strand at 439.950 kb on + strand at 440.008 kb on - strand at 440.008 kb on - strand at 440.008 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 438,207 + -1.2 438,212 + -3.1 438,212 + -0.3 438,212 + -0.9 438,212 + +0.6 438,213 - -0.4 438,239 - +2.2 438,263 + +0.8 438,263 + +1.2 438,264 - +0.9 438,264 - +1.6 438,316 - -0.4 438,477 + RPSI07_RS09890 0.25 +2.2 438,478 - RPSI07_RS09890 0.25 -3.1 438,478 - RPSI07_RS09890 0.25 +1.2 438,498 + RPSI07_RS09890 0.28 -1.2 438,498 + RPSI07_RS09890 0.28 +0.7 438,498 + RPSI07_RS09890 0.28 +1.2 438,499 - RPSI07_RS09890 0.28 +0.1 438,499 - RPSI07_RS09890 0.28 +0.2 438,499 - RPSI07_RS09890 0.28 +0.9 438,499 - RPSI07_RS09890 0.28 -0.4 438,499 - RPSI07_RS09890 0.28 +2.2 438,499 - RPSI07_RS09890 0.28 +0.2 438,828 + RPSI07_RS09890 0.71 -0.4 438,829 - RPSI07_RS09890 0.71 -0.2 438,829 - RPSI07_RS09890 0.71 -0.1 438,834 + RPSI07_RS09890 0.72 +1.5 439,076 - -0.7 439,076 - -0.9 439,076 - -0.5 439,076 - -0.3 439,362 + RPSI07_RS09895 0.22 -0.7 439,362 + RPSI07_RS09895 0.22 +0.2 439,362 + RPSI07_RS09895 0.22 +0.2 439,362 + RPSI07_RS09895 0.22 -0.8 439,362 + RPSI07_RS09895 0.22 +1.2 439,362 + RPSI07_RS09895 0.22 +2.5 439,363 - RPSI07_RS09895 0.23 -1.9 439,363 - RPSI07_RS09895 0.23 +1.2 439,363 - RPSI07_RS09895 0.23 +0.2 439,363 - RPSI07_RS09895 0.23 +0.6 439,363 - RPSI07_RS09895 0.23 -2.4 439,486 + RPSI07_RS09895 0.37 +0.2 439,486 + RPSI07_RS09895 0.37 +0.8 439,487 - RPSI07_RS09895 0.37 +0.9 439,487 - RPSI07_RS09895 0.37 +1.0 439,689 + RPSI07_RS09895 0.61 +1.1 439,690 - RPSI07_RS09895 0.61 +1.2 439,782 - RPSI07_RS09895 0.71 +2.7 439,787 + RPSI07_RS09895 0.72 -0.4 439,788 - RPSI07_RS09895 0.72 +0.4 439,788 - RPSI07_RS09895 0.72 +1.2 439,788 - RPSI07_RS09895 0.72 +1.2 439,950 + -2.3 439,950 + +0.1 440,008 - +2.5 440,008 - +3.2 440,008 - -1.2
Or see this region's nucleotide sequence