Experiment: SMSA no antibiotic control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS03235 and RPSI07_RS03240 are separated by 16 nucleotides RPSI07_RS03240 and RPSI07_RS03245 are separated by 193 nucleotides
RPSI07_RS03235: RPSI07_RS03235 - N-acetyl-gamma-glutamyl-phosphate reductase, at 883,983 to 884,918
_RS03235
RPSI07_RS03240: RPSI07_RS03240 - LysR family transcriptional regulator, at 884,935 to 885,873
_RS03240
RPSI07_RS03245: RPSI07_RS03245 - ABC transporter substrate-binding protein, at 886,067 to 887,611
_RS03245
Position (kb) 884
885
886 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 884.400 kb on + strand, within RPSI07_RS03235 at 884.463 kb on + strand, within RPSI07_RS03235 at 884.463 kb on + strand, within RPSI07_RS03235 at 884.463 kb on + strand, within RPSI07_RS03235 at 884.463 kb on + strand, within RPSI07_RS03235 at 884.464 kb on - strand, within RPSI07_RS03235 at 884.464 kb on - strand, within RPSI07_RS03235 at 884.464 kb on - strand, within RPSI07_RS03235 at 884.502 kb on + strand, within RPSI07_RS03235 at 884.502 kb on + strand, within RPSI07_RS03235 at 884.503 kb on - strand, within RPSI07_RS03235 at 884.503 kb on - strand, within RPSI07_RS03235 at 884.503 kb on - strand, within RPSI07_RS03235 at 884.586 kb on + strand, within RPSI07_RS03235 at 884.586 kb on + strand, within RPSI07_RS03235 at 884.586 kb on + strand, within RPSI07_RS03235 at 884.586 kb on + strand, within RPSI07_RS03235 at 884.587 kb on - strand, within RPSI07_RS03235 at 884.691 kb on + strand, within RPSI07_RS03235 at 884.937 kb on - strand at 884.937 kb on - strand at 885.182 kb on + strand, within RPSI07_RS03240 at 885.183 kb on - strand, within RPSI07_RS03240 at 885.183 kb on - strand, within RPSI07_RS03240 at 885.183 kb on - strand, within RPSI07_RS03240 at 885.183 kb on - strand, within RPSI07_RS03240 at 885.410 kb on + strand, within RPSI07_RS03240 at 885.411 kb on - strand, within RPSI07_RS03240 at 885.411 kb on - strand, within RPSI07_RS03240 at 885.411 kb on - strand, within RPSI07_RS03240 at 885.411 kb on - strand, within RPSI07_RS03240 at 885.506 kb on + strand, within RPSI07_RS03240 at 885.507 kb on - strand, within RPSI07_RS03240 at 885.507 kb on - strand, within RPSI07_RS03240 at 885.507 kb on - strand, within RPSI07_RS03240 at 885.507 kb on - strand, within RPSI07_RS03240 at 885.507 kb on - strand, within RPSI07_RS03240 at 885.507 kb on - strand, within RPSI07_RS03240 at 885.833 kb on + strand at 885.833 kb on + strand at 885.893 kb on + strand at 885.894 kb on - strand at 885.894 kb on - strand at 885.894 kb on - strand at 885.956 kb on + strand at 885.984 kb on + strand at 885.984 kb on + strand at 885.992 kb on + strand at 885.993 kb on - strand at 885.993 kb on - strand at 886.010 kb on + strand at 886.010 kb on + strand at 886.011 kb on - strand at 886.066 kb on + strand at 886.066 kb on + strand at 886.067 kb on - strand at 886.185 kb on - strand at 886.270 kb on + strand, within RPSI07_RS03245 at 886.271 kb on - strand, within RPSI07_RS03245 at 886.304 kb on + strand, within RPSI07_RS03245 at 886.433 kb on + strand, within RPSI07_RS03245 at 886.433 kb on + strand, within RPSI07_RS03245 at 886.433 kb on + strand, within RPSI07_RS03245 at 886.433 kb on + strand, within RPSI07_RS03245 at 886.433 kb on + strand, within RPSI07_RS03245 at 886.433 kb on + strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.559 kb on + strand, within RPSI07_RS03245 at 886.559 kb on + strand, within RPSI07_RS03245 at 886.560 kb on - strand, within RPSI07_RS03245 at 886.560 kb on - strand, within RPSI07_RS03245 at 886.560 kb on - strand, within RPSI07_RS03245 at 886.560 kb on - strand, within RPSI07_RS03245 at 886.652 kb on + strand, within RPSI07_RS03245 at 886.652 kb on + strand, within RPSI07_RS03245 at 886.653 kb on - strand, within RPSI07_RS03245 at 886.653 kb on - strand, within RPSI07_RS03245 at 886.653 kb on - strand, within RPSI07_RS03245 at 886.709 kb on + strand, within RPSI07_RS03245 at 886.709 kb on + strand, within RPSI07_RS03245 at 886.710 kb on - strand, within RPSI07_RS03245 at 886.710 kb on - strand, within RPSI07_RS03245 at 886.710 kb on - strand, within RPSI07_RS03245
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA no antibiotic control remove 884,400 + RPSI07_RS03235 0.45 +0.0 884,463 + RPSI07_RS03235 0.51 -0.5 884,463 + RPSI07_RS03235 0.51 +0.9 884,463 + RPSI07_RS03235 0.51 +0.5 884,463 + RPSI07_RS03235 0.51 -0.2 884,464 - RPSI07_RS03235 0.51 -0.2 884,464 - RPSI07_RS03235 0.51 +0.3 884,464 - RPSI07_RS03235 0.51 +0.4 884,502 + RPSI07_RS03235 0.55 +3.3 884,502 + RPSI07_RS03235 0.55 -1.0 884,503 - RPSI07_RS03235 0.56 -0.1 884,503 - RPSI07_RS03235 0.56 -0.5 884,503 - RPSI07_RS03235 0.56 -1.2 884,586 + RPSI07_RS03235 0.64 -1.1 884,586 + RPSI07_RS03235 0.64 +0.6 884,586 + RPSI07_RS03235 0.64 +1.1 884,586 + RPSI07_RS03235 0.64 -2.6 884,587 - RPSI07_RS03235 0.65 -0.7 884,691 + RPSI07_RS03235 0.76 +1.1 884,937 - -0.0 884,937 - -3.3 885,182 + RPSI07_RS03240 0.26 +0.2 885,183 - RPSI07_RS03240 0.26 +1.9 885,183 - RPSI07_RS03240 0.26 -2.2 885,183 - RPSI07_RS03240 0.26 +0.6 885,183 - RPSI07_RS03240 0.26 -1.1 885,410 + RPSI07_RS03240 0.51 -1.4 885,411 - RPSI07_RS03240 0.51 -0.2 885,411 - RPSI07_RS03240 0.51 -1.1 885,411 - RPSI07_RS03240 0.51 +0.5 885,411 - RPSI07_RS03240 0.51 +0.3 885,506 + RPSI07_RS03240 0.61 -0.3 885,507 - RPSI07_RS03240 0.61 +4.2 885,507 - RPSI07_RS03240 0.61 +0.4 885,507 - RPSI07_RS03240 0.61 -1.4 885,507 - RPSI07_RS03240 0.61 +0.8 885,507 - RPSI07_RS03240 0.61 -0.3 885,507 - RPSI07_RS03240 0.61 +0.5 885,833 + -1.9 885,833 + -0.8 885,893 + -2.0 885,894 - -1.4 885,894 - +0.7 885,894 - -2.6 885,956 + +0.4 885,984 + -1.2 885,984 + -3.6 885,992 + +0.6 885,993 - -0.6 885,993 - -1.5 886,010 + -0.9 886,010 + +0.3 886,011 - -2.6 886,066 + +0.7 886,066 + -1.0 886,067 - -1.0 886,185 - -0.0 886,270 + RPSI07_RS03245 0.13 -0.4 886,271 - RPSI07_RS03245 0.13 -0.0 886,304 + RPSI07_RS03245 0.15 +2.5 886,433 + RPSI07_RS03245 0.24 +0.1 886,433 + RPSI07_RS03245 0.24 +0.3 886,433 + RPSI07_RS03245 0.24 +0.2 886,433 + RPSI07_RS03245 0.24 +0.5 886,433 + RPSI07_RS03245 0.24 -2.2 886,433 + RPSI07_RS03245 0.24 -0.7 886,434 - RPSI07_RS03245 0.24 +0.4 886,434 - RPSI07_RS03245 0.24 +0.3 886,434 - RPSI07_RS03245 0.24 -0.5 886,434 - RPSI07_RS03245 0.24 -0.6 886,434 - RPSI07_RS03245 0.24 +1.6 886,434 - RPSI07_RS03245 0.24 +0.1 886,434 - RPSI07_RS03245 0.24 -1.9 886,434 - RPSI07_RS03245 0.24 -1.5 886,434 - RPSI07_RS03245 0.24 -0.1 886,559 + RPSI07_RS03245 0.32 -0.5 886,559 + RPSI07_RS03245 0.32 -2.1 886,560 - RPSI07_RS03245 0.32 +0.4 886,560 - RPSI07_RS03245 0.32 +0.5 886,560 - RPSI07_RS03245 0.32 +0.2 886,560 - RPSI07_RS03245 0.32 -0.6 886,652 + RPSI07_RS03245 0.38 -0.1 886,652 + RPSI07_RS03245 0.38 -0.1 886,653 - RPSI07_RS03245 0.38 -0.7 886,653 - RPSI07_RS03245 0.38 +0.2 886,653 - RPSI07_RS03245 0.38 +0.5 886,709 + RPSI07_RS03245 0.42 -0.7 886,709 + RPSI07_RS03245 0.42 -0.8 886,710 - RPSI07_RS03245 0.42 -1.0 886,710 - RPSI07_RS03245 0.42 -2.1 886,710 - RPSI07_RS03245 0.42 -0.6
Or see this region's nucleotide sequence