Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21630 and RPSI07_RS21635 are separated by 298 nucleotides RPSI07_RS21635 and RPSI07_RS21640 are separated by 66 nucleotides RPSI07_RS21640 and RPSI07_RS21645 overlap by 4 nucleotides
RPSI07_RS21630: RPSI07_RS21630 - glycerate kinase, at 3,011,382 to 3,012,680
_RS21630
RPSI07_RS21635: RPSI07_RS21635 - class II glutamine amidotransferase, at 3,012,979 to 3,013,809
_RS21635
RPSI07_RS21640: RPSI07_RS21640 - amino acid ABC transporter ATP-binding protein, at 3,013,876 to 3,014,610
_RS21640
RPSI07_RS21645: RPSI07_RS21645 - glutamate/aspartate transporter permease GltK, at 3,014,607 to 3,015,290
_RS21645
Position (kb)
3012
3013
3014 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3012.050 kb on - strand, within RPSI07_RS21630 at 3012.454 kb on + strand, within RPSI07_RS21630 at 3012.454 kb on + strand, within RPSI07_RS21630 at 3012.497 kb on - strand, within RPSI07_RS21630 at 3012.497 kb on - strand, within RPSI07_RS21630 at 3012.497 kb on - strand, within RPSI07_RS21630 at 3012.942 kb on - strand at 3012.948 kb on + strand at 3012.949 kb on - strand at 3012.953 kb on + strand at 3012.954 kb on - strand at 3013.215 kb on + strand, within RPSI07_RS21635 at 3013.215 kb on + strand, within RPSI07_RS21635 at 3013.447 kb on + strand, within RPSI07_RS21635 at 3013.447 kb on + strand, within RPSI07_RS21635 at 3013.448 kb on - strand, within RPSI07_RS21635 at 3013.498 kb on + strand, within RPSI07_RS21635 at 3013.498 kb on + strand, within RPSI07_RS21635 at 3013.499 kb on - strand, within RPSI07_RS21635 at 3013.499 kb on - strand, within RPSI07_RS21635 at 3013.549 kb on + strand, within RPSI07_RS21635 at 3013.549 kb on + strand, within RPSI07_RS21635 at 3013.550 kb on - strand, within RPSI07_RS21635 at 3013.552 kb on + strand, within RPSI07_RS21635 at 3013.552 kb on + strand, within RPSI07_RS21635 at 3013.624 kb on + strand, within RPSI07_RS21635 at 3013.624 kb on + strand, within RPSI07_RS21635 at 3013.624 kb on + strand, within RPSI07_RS21635 at 3013.625 kb on - strand, within RPSI07_RS21635 at 3013.811 kb on - strand at 3014.252 kb on + strand, within RPSI07_RS21640 at 3014.252 kb on + strand, within RPSI07_RS21640 at 3014.252 kb on + strand, within RPSI07_RS21640 at 3014.253 kb on - strand, within RPSI07_RS21640 at 3014.253 kb on - strand, within RPSI07_RS21640 at 3014.579 kb on + strand at 3014.579 kb on + strand at 3014.580 kb on - strand at 3014.671 kb on + strand at 3014.671 kb on + strand at 3014.672 kb on - strand at 3014.672 kb on - strand at 3014.672 kb on - strand at 3014.675 kb on + strand at 3014.675 kb on + strand at 3014.675 kb on + strand at 3014.675 kb on + strand at 3014.675 kb on + strand at 3014.675 kb on + strand at 3014.676 kb on - strand, within RPSI07_RS21645 at 3014.676 kb on - strand, within RPSI07_RS21645 at 3014.676 kb on - strand, within RPSI07_RS21645 at 3014.731 kb on + strand, within RPSI07_RS21645 at 3014.731 kb on + strand, within RPSI07_RS21645 at 3014.732 kb on - strand, within RPSI07_RS21645 at 3014.741 kb on + strand, within RPSI07_RS21645 at 3014.767 kb on + strand, within RPSI07_RS21645 at 3014.767 kb on + strand, within RPSI07_RS21645 at 3014.780 kb on + strand, within RPSI07_RS21645 at 3014.780 kb on + strand, within RPSI07_RS21645
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 3,012,050 - RPSI07_RS21630 0.51 -0.1 3,012,454 + RPSI07_RS21630 0.83 +1.1 3,012,454 + RPSI07_RS21630 0.83 +0.6 3,012,497 - RPSI07_RS21630 0.86 +0.1 3,012,497 - RPSI07_RS21630 0.86 +0.1 3,012,497 - RPSI07_RS21630 0.86 -1.0 3,012,942 - -0.9 3,012,948 + +1.1 3,012,949 - +0.9 3,012,953 + +0.1 3,012,954 - +1.7 3,013,215 + RPSI07_RS21635 0.28 -0.5 3,013,215 + RPSI07_RS21635 0.28 -2.1 3,013,447 + RPSI07_RS21635 0.56 -0.4 3,013,447 + RPSI07_RS21635 0.56 -0.1 3,013,448 - RPSI07_RS21635 0.56 -1.8 3,013,498 + RPSI07_RS21635 0.62 +3.1 3,013,498 + RPSI07_RS21635 0.62 -0.6 3,013,499 - RPSI07_RS21635 0.63 +2.1 3,013,499 - RPSI07_RS21635 0.63 -0.5 3,013,549 + RPSI07_RS21635 0.69 +2.1 3,013,549 + RPSI07_RS21635 0.69 +0.0 3,013,550 - RPSI07_RS21635 0.69 -1.4 3,013,552 + RPSI07_RS21635 0.69 -0.8 3,013,552 + RPSI07_RS21635 0.69 -1.8 3,013,624 + RPSI07_RS21635 0.78 +1.1 3,013,624 + RPSI07_RS21635 0.78 +3.4 3,013,624 + RPSI07_RS21635 0.78 +1.6 3,013,625 - RPSI07_RS21635 0.78 +1.1 3,013,811 - -0.7 3,014,252 + RPSI07_RS21640 0.51 +1.1 3,014,252 + RPSI07_RS21640 0.51 -2.1 3,014,252 + RPSI07_RS21640 0.51 +1.1 3,014,253 - RPSI07_RS21640 0.51 -1.3 3,014,253 - RPSI07_RS21640 0.51 -2.0 3,014,579 + +1.1 3,014,579 + -1.3 3,014,580 - +1.1 3,014,671 + -0.4 3,014,671 + -0.4 3,014,672 - -2.3 3,014,672 - +1.4 3,014,672 - -0.7 3,014,675 + +2.1 3,014,675 + -0.0 3,014,675 + +0.5 3,014,675 + -0.9 3,014,675 + -2.0 3,014,675 + +0.6 3,014,676 - RPSI07_RS21645 0.10 -1.1 3,014,676 - RPSI07_RS21645 0.10 -1.6 3,014,676 - RPSI07_RS21645 0.10 +1.1 3,014,731 + RPSI07_RS21645 0.18 -0.3 3,014,731 + RPSI07_RS21645 0.18 -0.9 3,014,732 - RPSI07_RS21645 0.18 -2.4 3,014,741 + RPSI07_RS21645 0.20 +0.6 3,014,767 + RPSI07_RS21645 0.23 -0.2 3,014,767 + RPSI07_RS21645 0.23 -0.3 3,014,780 + RPSI07_RS21645 0.25 -0.3 3,014,780 + RPSI07_RS21645 0.25 +1.1
Or see this region's nucleotide sequence