Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS21305

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS21295 and RPSI07_RS21300 are separated by 21 nucleotidesRPSI07_RS21300 and RPSI07_RS21305 are separated by 68 nucleotidesRPSI07_RS21305 and RPSI07_RS21310 are separated by 443 nucleotides RPSI07_RS21295: RPSI07_RS21295 - FAD-binding monooxygenase, at 2,939,576 to 2,940,772 _RS21295 RPSI07_RS21300: RPSI07_RS21300 - hypothetical protein, at 2,940,794 to 2,941,354 _RS21300 RPSI07_RS21305: RPSI07_RS21305 - DNA-binding response regulator, at 2,941,423 to 2,942,163 _RS21305 RPSI07_RS21310: RPSI07_RS21310 - LysR family transcriptional regulator, at 2,942,607 to 2,943,524 _RS21310 Position (kb) 2941 2942 2943Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2940.581 kb on + strand, within RPSI07_RS21295at 2940.582 kb on - strand, within RPSI07_RS21295at 2941.274 kb on + strand, within RPSI07_RS21300at 2941.275 kb on - strand, within RPSI07_RS21300at 2941.352 kb on + strandat 2941.352 kb on + strandat 2941.353 kb on - strandat 2941.353 kb on - strandat 2941.425 kb on - strandat 2941.500 kb on - strand, within RPSI07_RS21305at 2941.511 kb on + strand, within RPSI07_RS21305at 2941.511 kb on + strand, within RPSI07_RS21305at 2941.512 kb on - strand, within RPSI07_RS21305at 2941.512 kb on - strand, within RPSI07_RS21305at 2941.512 kb on - strand, within RPSI07_RS21305at 2941.673 kb on - strand, within RPSI07_RS21305at 2941.673 kb on - strand, within RPSI07_RS21305at 2941.673 kb on - strand, within RPSI07_RS21305at 2941.791 kb on - strand, within RPSI07_RS21305at 2941.868 kb on + strand, within RPSI07_RS21305at 2941.869 kb on - strand, within RPSI07_RS21305at 2941.869 kb on - strand, within RPSI07_RS21305at 2942.030 kb on + strand, within RPSI07_RS21305at 2942.031 kb on - strand, within RPSI07_RS21305at 2942.157 kb on + strandat 2942.800 kb on + strand, within RPSI07_RS21310at 2942.801 kb on - strand, within RPSI07_RS21310at 2942.801 kb on - strand, within RPSI07_RS21310at 2942.851 kb on + strand, within RPSI07_RS21310at 2942.852 kb on - strand, within RPSI07_RS21310at 2942.852 kb on - strand, within RPSI07_RS21310at 2943.016 kb on + strand, within RPSI07_RS21310at 2943.016 kb on + strand, within RPSI07_RS21310at 2943.016 kb on + strand, within RPSI07_RS21310at 2943.017 kb on - strand, within RPSI07_RS21310

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
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2,940,581 + RPSI07_RS21295 0.84 +1.2
2,940,582 - RPSI07_RS21295 0.84 -1.0
2,941,274 + RPSI07_RS21300 0.86 +0.5
2,941,275 - RPSI07_RS21300 0.86 -1.3
2,941,352 + +0.4
2,941,352 + -0.3
2,941,353 - -0.4
2,941,353 - -2.7
2,941,425 - -1.0
2,941,500 - RPSI07_RS21305 0.10 -0.1
2,941,511 + RPSI07_RS21305 0.12 -0.2
2,941,511 + RPSI07_RS21305 0.12 -1.4
2,941,512 - RPSI07_RS21305 0.12 -1.9
2,941,512 - RPSI07_RS21305 0.12 -1.0
2,941,512 - RPSI07_RS21305 0.12 -1.1
2,941,673 - RPSI07_RS21305 0.34 -0.2
2,941,673 - RPSI07_RS21305 0.34 -0.9
2,941,673 - RPSI07_RS21305 0.34 -0.8
2,941,791 - RPSI07_RS21305 0.50 +0.3
2,941,868 + RPSI07_RS21305 0.60 -0.9
2,941,869 - RPSI07_RS21305 0.60 +1.1
2,941,869 - RPSI07_RS21305 0.60 +0.2
2,942,030 + RPSI07_RS21305 0.82 +0.1
2,942,031 - RPSI07_RS21305 0.82 +1.1
2,942,157 + +3.1
2,942,800 + RPSI07_RS21310 0.21 -1.2
2,942,801 - RPSI07_RS21310 0.21 +0.7
2,942,801 - RPSI07_RS21310 0.21 +2.1
2,942,851 + RPSI07_RS21310 0.27 +0.5
2,942,852 - RPSI07_RS21310 0.27 +2.1
2,942,852 - RPSI07_RS21310 0.27 -0.4
2,943,016 + RPSI07_RS21310 0.45 +0.0
2,943,016 + RPSI07_RS21310 0.45 -0.1
2,943,016 + RPSI07_RS21310 0.45 -0.9
2,943,017 - RPSI07_RS21310 0.45 -1.9

Or see this region's nucleotide sequence