Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21295 and RPSI07_RS21300 are separated by 21 nucleotides RPSI07_RS21300 and RPSI07_RS21305 are separated by 68 nucleotides
RPSI07_RS21295: RPSI07_RS21295 - FAD-binding monooxygenase, at 2,939,576 to 2,940,772
_RS21295
RPSI07_RS21300: RPSI07_RS21300 - hypothetical protein, at 2,940,794 to 2,941,354
_RS21300
RPSI07_RS21305: RPSI07_RS21305 - DNA-binding response regulator, at 2,941,423 to 2,942,163
_RS21305
Position (kb)
2940
2941
2942 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2939.970 kb on - strand, within RPSI07_RS21295 at 2940.092 kb on + strand, within RPSI07_RS21295 at 2940.416 kb on + strand, within RPSI07_RS21295 at 2940.417 kb on - strand, within RPSI07_RS21295 at 2940.581 kb on + strand, within RPSI07_RS21295 at 2940.582 kb on - strand, within RPSI07_RS21295 at 2941.274 kb on + strand, within RPSI07_RS21300 at 2941.275 kb on - strand, within RPSI07_RS21300 at 2941.352 kb on + strand at 2941.352 kb on + strand at 2941.353 kb on - strand at 2941.353 kb on - strand at 2941.425 kb on - strand at 2941.500 kb on - strand, within RPSI07_RS21305 at 2941.511 kb on + strand, within RPSI07_RS21305 at 2941.511 kb on + strand, within RPSI07_RS21305 at 2941.512 kb on - strand, within RPSI07_RS21305 at 2941.512 kb on - strand, within RPSI07_RS21305 at 2941.512 kb on - strand, within RPSI07_RS21305 at 2941.673 kb on - strand, within RPSI07_RS21305 at 2941.673 kb on - strand, within RPSI07_RS21305 at 2941.673 kb on - strand, within RPSI07_RS21305 at 2941.791 kb on - strand, within RPSI07_RS21305 at 2941.868 kb on + strand, within RPSI07_RS21305 at 2941.869 kb on - strand, within RPSI07_RS21305 at 2941.869 kb on - strand, within RPSI07_RS21305 at 2942.030 kb on + strand, within RPSI07_RS21305 at 2942.031 kb on - strand, within RPSI07_RS21305 at 2942.157 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 2,939,970 - RPSI07_RS21295 0.33 -2.2 2,940,092 + RPSI07_RS21295 0.43 +3.0 2,940,416 + RPSI07_RS21295 0.70 -1.2 2,940,417 - RPSI07_RS21295 0.70 -0.1 2,940,581 + RPSI07_RS21295 0.84 +1.2 2,940,582 - RPSI07_RS21295 0.84 -1.0 2,941,274 + RPSI07_RS21300 0.86 +0.5 2,941,275 - RPSI07_RS21300 0.86 -1.3 2,941,352 + +0.4 2,941,352 + -0.3 2,941,353 - -0.4 2,941,353 - -2.7 2,941,425 - -1.0 2,941,500 - RPSI07_RS21305 0.10 -0.1 2,941,511 + RPSI07_RS21305 0.12 -0.2 2,941,511 + RPSI07_RS21305 0.12 -1.4 2,941,512 - RPSI07_RS21305 0.12 -1.9 2,941,512 - RPSI07_RS21305 0.12 -1.0 2,941,512 - RPSI07_RS21305 0.12 -1.1 2,941,673 - RPSI07_RS21305 0.34 -0.2 2,941,673 - RPSI07_RS21305 0.34 -0.9 2,941,673 - RPSI07_RS21305 0.34 -0.8 2,941,791 - RPSI07_RS21305 0.50 +0.3 2,941,868 + RPSI07_RS21305 0.60 -0.9 2,941,869 - RPSI07_RS21305 0.60 +1.1 2,941,869 - RPSI07_RS21305 0.60 +0.2 2,942,030 + RPSI07_RS21305 0.82 +0.1 2,942,031 - RPSI07_RS21305 0.82 +1.1 2,942,157 + +3.1
Or see this region's nucleotide sequence