Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21025 and RPSI07_RS21030 are separated by 78 nucleotides RPSI07_RS21030 and RPSI07_RS21035 are separated by 155 nucleotides RPSI07_RS21035 and RPSI07_RS21040 are separated by 434 nucleotides
RPSI07_RS21025: RPSI07_RS21025 - TonB-dependent receptor, at 2,881,490 to 2,883,679
_RS21025
RPSI07_RS21030: RPSI07_RS21030 - hypothetical protein, at 2,883,758 to 2,884,135
_RS21030
RPSI07_RS21035: RPSI07_RS21035 - hypothetical protein, at 2,884,291 to 2,885,277
_RS21035
RPSI07_RS21040: RPSI07_RS21040 - HD-GYP domain-containing protein, at 2,885,712 to 2,886,923
_RS21040
Position (kb)
2884
2885
2886 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2884.202 kb on + strand at 2884.790 kb on + strand, within RPSI07_RS21035 at 2884.790 kb on + strand, within RPSI07_RS21035 at 2884.790 kb on + strand, within RPSI07_RS21035 at 2884.791 kb on - strand, within RPSI07_RS21035 at 2884.791 kb on - strand, within RPSI07_RS21035 at 2884.817 kb on + strand, within RPSI07_RS21035 at 2884.818 kb on - strand, within RPSI07_RS21035 at 2884.818 kb on - strand, within RPSI07_RS21035 at 2885.387 kb on + strand at 2885.387 kb on + strand at 2885.439 kb on - strand at 2885.475 kb on + strand at 2885.476 kb on - strand at 2885.527 kb on + strand at 2885.527 kb on + strand at 2885.527 kb on + strand at 2885.527 kb on + strand at 2885.528 kb on - strand at 2885.848 kb on + strand, within RPSI07_RS21040 at 2885.848 kb on + strand, within RPSI07_RS21040 at 2885.849 kb on - strand, within RPSI07_RS21040 at 2885.849 kb on - strand, within RPSI07_RS21040 at 2885.849 kb on - strand, within RPSI07_RS21040 at 2885.849 kb on - strand, within RPSI07_RS21040 at 2885.966 kb on + strand, within RPSI07_RS21040 at 2886.019 kb on + strand, within RPSI07_RS21040 at 2886.019 kb on + strand, within RPSI07_RS21040 at 2886.019 kb on + strand, within RPSI07_RS21040 at 2886.019 kb on + strand, within RPSI07_RS21040 at 2886.019 kb on + strand, within RPSI07_RS21040 at 2886.019 kb on + strand, within RPSI07_RS21040 at 2886.020 kb on - strand, within RPSI07_RS21040 at 2886.020 kb on - strand, within RPSI07_RS21040 at 2886.055 kb on + strand, within RPSI07_RS21040 at 2886.055 kb on + strand, within RPSI07_RS21040 at 2886.056 kb on - strand, within RPSI07_RS21040 at 2886.056 kb on - strand, within RPSI07_RS21040 at 2886.082 kb on + strand, within RPSI07_RS21040 at 2886.082 kb on + strand, within RPSI07_RS21040 at 2886.082 kb on + strand, within RPSI07_RS21040 at 2886.083 kb on - strand, within RPSI07_RS21040
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 2,884,202 + +0.6 2,884,790 + RPSI07_RS21035 0.51 -1.4 2,884,790 + RPSI07_RS21035 0.51 +0.2 2,884,790 + RPSI07_RS21035 0.51 -0.5 2,884,791 - RPSI07_RS21035 0.51 +1.0 2,884,791 - RPSI07_RS21035 0.51 +2.1 2,884,817 + RPSI07_RS21035 0.53 +0.1 2,884,818 - RPSI07_RS21035 0.53 -0.9 2,884,818 - RPSI07_RS21035 0.53 -0.5 2,885,387 + -1.3 2,885,387 + +1.7 2,885,439 - +0.3 2,885,475 + +1.1 2,885,476 - +0.1 2,885,527 + -0.1 2,885,527 + +0.1 2,885,527 + +1.0 2,885,527 + +2.1 2,885,528 - -0.1 2,885,848 + RPSI07_RS21040 0.11 +0.2 2,885,848 + RPSI07_RS21040 0.11 -0.4 2,885,849 - RPSI07_RS21040 0.11 -0.3 2,885,849 - RPSI07_RS21040 0.11 -1.5 2,885,849 - RPSI07_RS21040 0.11 +2.4 2,885,849 - RPSI07_RS21040 0.11 -0.9 2,885,966 + RPSI07_RS21040 0.21 -0.7 2,886,019 + RPSI07_RS21040 0.25 +0.4 2,886,019 + RPSI07_RS21040 0.25 +1.7 2,886,019 + RPSI07_RS21040 0.25 -0.8 2,886,019 + RPSI07_RS21040 0.25 +0.9 2,886,019 + RPSI07_RS21040 0.25 -0.7 2,886,019 + RPSI07_RS21040 0.25 +3.7 2,886,020 - RPSI07_RS21040 0.25 -0.5 2,886,020 - RPSI07_RS21040 0.25 +1.1 2,886,055 + RPSI07_RS21040 0.28 +2.2 2,886,055 + RPSI07_RS21040 0.28 -0.4 2,886,056 - RPSI07_RS21040 0.28 +2.1 2,886,056 - RPSI07_RS21040 0.28 -0.4 2,886,082 + RPSI07_RS21040 0.31 +0.1 2,886,082 + RPSI07_RS21040 0.31 +1.1 2,886,082 + RPSI07_RS21040 0.31 +0.5 2,886,083 - RPSI07_RS21040 0.31 -1.0
Or see this region's nucleotide sequence