Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS20265 and RPSI07_RS20270 are separated by 252 nucleotides RPSI07_RS20270 and RPSI07_RS20275 are separated by 535 nucleotides RPSI07_RS20275 and RPSI07_RS20280 are separated by 61 nucleotides
RPSI07_RS20265: RPSI07_RS20265 - DNA/RNA non-specific endonuclease, at 2,727,667 to 2,728,494
_RS20265
RPSI07_RS20270: RPSI07_RS20270 - 3-deoxy-7-phosphoheptulonate synthase, at 2,728,747 to 2,729,865
_RS20270
RPSI07_RS20275: RPSI07_RS20275 - tRNA-Lys, at 2,730,401 to 2,730,476
_RS20275
RPSI07_RS20280: RPSI07_RS20280 - YeeE/YedE family protein, at 2,730,538 to 2,731,653
_RS20280
Position (kb)
2728
2729
2730 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2727.934 kb on + strand, within RPSI07_RS20265 at 2727.934 kb on + strand, within RPSI07_RS20265 at 2727.935 kb on - strand, within RPSI07_RS20265 at 2727.935 kb on - strand, within RPSI07_RS20265 at 2727.961 kb on + strand, within RPSI07_RS20265 at 2728.078 kb on + strand, within RPSI07_RS20265 at 2728.079 kb on - strand, within RPSI07_RS20265 at 2728.079 kb on - strand, within RPSI07_RS20265 at 2728.079 kb on - strand, within RPSI07_RS20265 at 2728.237 kb on + strand, within RPSI07_RS20265 at 2728.264 kb on + strand, within RPSI07_RS20265 at 2728.264 kb on + strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.265 kb on - strand, within RPSI07_RS20265 at 2728.336 kb on + strand, within RPSI07_RS20265 at 2728.337 kb on - strand, within RPSI07_RS20265 at 2728.342 kb on + strand, within RPSI07_RS20265 at 2728.342 kb on + strand, within RPSI07_RS20265 at 2728.416 kb on - strand at 2729.081 kb on + strand, within RPSI07_RS20270 at 2729.081 kb on + strand, within RPSI07_RS20270 at 2729.081 kb on + strand, within RPSI07_RS20270 at 2729.082 kb on - strand, within RPSI07_RS20270 at 2729.351 kb on + strand, within RPSI07_RS20270 at 2729.351 kb on + strand, within RPSI07_RS20270 at 2729.486 kb on + strand, within RPSI07_RS20270 at 2729.729 kb on - strand, within RPSI07_RS20270 at 2729.741 kb on - strand, within RPSI07_RS20270 at 2729.802 kb on + strand at 2729.802 kb on + strand at 2729.802 kb on + strand at 2729.990 kb on + strand at 2729.990 kb on + strand at 2729.990 kb on + strand at 2729.991 kb on - strand at 2730.054 kb on + strand at 2730.055 kb on - strand at 2730.087 kb on + strand at 2730.088 kb on - strand at 2730.088 kb on - strand at 2730.173 kb on + strand at 2730.281 kb on + strand at 2730.281 kb on + strand at 2730.282 kb on - strand at 2730.282 kb on - strand at 2730.282 kb on - strand at 2730.282 kb on - strand at 2730.282 kb on - strand at 2730.282 kb on - strand at 2730.285 kb on + strand at 2730.286 kb on - strand at 2730.286 kb on - strand at 2730.286 kb on - strand at 2730.286 kb on - strand at 2730.286 kb on - strand at 2730.303 kb on + strand at 2730.304 kb on - strand at 2730.325 kb on + strand at 2730.325 kb on + strand at 2730.325 kb on + strand at 2730.325 kb on + strand at 2730.326 kb on - strand at 2730.326 kb on - strand at 2730.326 kb on - strand at 2730.326 kb on - strand at 2730.326 kb on - strand at 2730.330 kb on + strand at 2730.330 kb on + strand at 2730.330 kb on + strand at 2730.331 kb on - strand at 2730.331 kb on - strand at 2730.396 kb on + strand at 2730.492 kb on - strand at 2730.666 kb on + strand, within RPSI07_RS20280 at 2730.717 kb on + strand, within RPSI07_RS20280 at 2730.718 kb on - strand, within RPSI07_RS20280
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 2,727,934 + RPSI07_RS20265 0.32 +0.2 2,727,934 + RPSI07_RS20265 0.32 -0.9 2,727,935 - RPSI07_RS20265 0.32 -0.3 2,727,935 - RPSI07_RS20265 0.32 -1.4 2,727,961 + RPSI07_RS20265 0.36 +1.7 2,728,078 + RPSI07_RS20265 0.50 -1.3 2,728,079 - RPSI07_RS20265 0.50 +0.5 2,728,079 - RPSI07_RS20265 0.50 +1.6 2,728,079 - RPSI07_RS20265 0.50 +0.0 2,728,237 + RPSI07_RS20265 0.69 -2.7 2,728,264 + RPSI07_RS20265 0.72 -1.0 2,728,264 + RPSI07_RS20265 0.72 +1.6 2,728,265 - RPSI07_RS20265 0.72 -2.2 2,728,265 - RPSI07_RS20265 0.72 -3.2 2,728,265 - RPSI07_RS20265 0.72 -0.2 2,728,265 - RPSI07_RS20265 0.72 -0.6 2,728,265 - RPSI07_RS20265 0.72 -0.1 2,728,265 - RPSI07_RS20265 0.72 -0.6 2,728,265 - RPSI07_RS20265 0.72 +0.5 2,728,265 - RPSI07_RS20265 0.72 +0.5 2,728,265 - RPSI07_RS20265 0.72 -0.3 2,728,265 - RPSI07_RS20265 0.72 +0.5 2,728,336 + RPSI07_RS20265 0.81 +1.5 2,728,337 - RPSI07_RS20265 0.81 -1.9 2,728,342 + RPSI07_RS20265 0.82 -0.5 2,728,342 + RPSI07_RS20265 0.82 -1.0 2,728,416 - +0.9 2,729,081 + RPSI07_RS20270 0.30 +1.0 2,729,081 + RPSI07_RS20270 0.30 -3.2 2,729,081 + RPSI07_RS20270 0.30 +0.0 2,729,082 - RPSI07_RS20270 0.30 +0.3 2,729,351 + RPSI07_RS20270 0.54 +0.0 2,729,351 + RPSI07_RS20270 0.54 -1.9 2,729,486 + RPSI07_RS20270 0.66 -1.1 2,729,729 - RPSI07_RS20270 0.88 -2.1 2,729,741 - RPSI07_RS20270 0.89 -0.3 2,729,802 + +0.8 2,729,802 + -1.1 2,729,802 + -0.3 2,729,990 + -0.0 2,729,990 + +1.0 2,729,990 + -0.8 2,729,991 - +0.0 2,730,054 + -0.8 2,730,055 - +1.1 2,730,087 + -1.3 2,730,088 - -0.3 2,730,088 - +1.0 2,730,173 + +0.5 2,730,281 + -0.3 2,730,281 + -0.3 2,730,282 - -1.8 2,730,282 - -1.6 2,730,282 - -0.8 2,730,282 - +0.7 2,730,282 - -0.6 2,730,282 - -0.8 2,730,285 + +0.1 2,730,286 - +0.3 2,730,286 - +2.1 2,730,286 - -2.2 2,730,286 - +2.0 2,730,286 - -0.0 2,730,303 + +0.5 2,730,304 - -1.4 2,730,325 + -1.9 2,730,325 + -2.3 2,730,325 + -0.6 2,730,325 + -0.2 2,730,326 - +0.5 2,730,326 - +0.8 2,730,326 - +1.0 2,730,326 - +0.6 2,730,326 - -1.7 2,730,330 + -0.5 2,730,330 + -0.2 2,730,330 + +3.0 2,730,331 - -1.9 2,730,331 - +0.3 2,730,396 + -0.2 2,730,492 - +0.0 2,730,666 + RPSI07_RS20280 0.11 -1.0 2,730,717 + RPSI07_RS20280 0.16 +2.0 2,730,718 - RPSI07_RS20280 0.16 +1.6
Or see this region's nucleotide sequence