Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS18440 and RPSI07_RS18445 overlap by 1 nucleotides RPSI07_RS18445 and RPSI07_RS18450 are separated by 55 nucleotides RPSI07_RS18450 and RPSI07_RS18455 are separated by 11 nucleotides
RPSI07_RS18440: RPSI07_RS18440 - DNA mismatch repair protein MutS, at 2,338,304 to 2,340,952
_RS18440
RPSI07_RS18445: RPSI07_RS18445 - hypothetical protein, at 2,340,952 to 2,342,019
_RS18445
RPSI07_RS18450: RPSI07_RS18450 - peptidylprolyl isomerase, at 2,342,075 to 2,342,599
_RS18450
RPSI07_RS18455: RPSI07_RS18455 - cupin domain-containing protein, at 2,342,611 to 2,343,840
_RS18455
Position (kb)
2340
2341
2342
2343 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2339.998 kb on - strand, within RPSI07_RS18440 at 2340.045 kb on + strand, within RPSI07_RS18440 at 2340.205 kb on + strand, within RPSI07_RS18440 at 2340.252 kb on - strand, within RPSI07_RS18440 at 2340.417 kb on + strand, within RPSI07_RS18440 at 2340.418 kb on - strand, within RPSI07_RS18440 at 2340.659 kb on + strand, within RPSI07_RS18440 at 2340.660 kb on - strand, within RPSI07_RS18440 at 2340.660 kb on - strand, within RPSI07_RS18440 at 2341.282 kb on + strand, within RPSI07_RS18445 at 2341.282 kb on + strand, within RPSI07_RS18445 at 2341.282 kb on + strand at 2341.283 kb on - strand, within RPSI07_RS18445 at 2341.283 kb on - strand, within RPSI07_RS18445 at 2341.283 kb on - strand, within RPSI07_RS18445 at 2341.283 kb on - strand, within RPSI07_RS18445 at 2341.283 kb on - strand, within RPSI07_RS18445 at 2341.454 kb on - strand, within RPSI07_RS18445 at 2341.528 kb on + strand, within RPSI07_RS18445 at 2341.528 kb on + strand, within RPSI07_RS18445 at 2341.529 kb on - strand, within RPSI07_RS18445 at 2341.742 kb on - strand, within RPSI07_RS18445 at 2341.797 kb on - strand, within RPSI07_RS18445 at 2342.053 kb on + strand at 2342.053 kb on + strand at 2342.061 kb on - strand at 2342.277 kb on + strand, within RPSI07_RS18450 at 2342.391 kb on + strand, within RPSI07_RS18450 at 2342.505 kb on + strand, within RPSI07_RS18450 at 2342.506 kb on - strand, within RPSI07_RS18450 at 2342.506 kb on - strand at 2342.560 kb on + strand at 2342.560 kb on + strand at 2342.560 kb on + strand at 2342.561 kb on - strand at 2342.562 kb on + strand at 2342.582 kb on - strand at 2342.628 kb on - strand at 2342.629 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 2,339,998 - RPSI07_RS18440 0.64 +0.4 2,340,045 + RPSI07_RS18440 0.66 -0.2 2,340,205 + RPSI07_RS18440 0.72 +0.3 2,340,252 - RPSI07_RS18440 0.74 -0.0 2,340,417 + RPSI07_RS18440 0.80 +0.1 2,340,418 - RPSI07_RS18440 0.80 +2.0 2,340,659 + RPSI07_RS18440 0.89 +0.4 2,340,660 - RPSI07_RS18440 0.89 -0.7 2,340,660 - RPSI07_RS18440 0.89 +0.2 2,341,282 + RPSI07_RS18445 0.31 -0.8 2,341,282 + RPSI07_RS18445 0.31 -1.0 2,341,282 + +1.0 2,341,283 - RPSI07_RS18445 0.31 -0.6 2,341,283 - RPSI07_RS18445 0.31 +0.3 2,341,283 - RPSI07_RS18445 0.31 -4.3 2,341,283 - RPSI07_RS18445 0.31 -1.3 2,341,283 - RPSI07_RS18445 0.31 +2.6 2,341,454 - RPSI07_RS18445 0.47 -0.9 2,341,528 + RPSI07_RS18445 0.54 +1.0 2,341,528 + RPSI07_RS18445 0.54 -0.2 2,341,529 - RPSI07_RS18445 0.54 -0.3 2,341,742 - RPSI07_RS18445 0.74 +1.1 2,341,797 - RPSI07_RS18445 0.79 +0.4 2,342,053 + +0.6 2,342,053 + -1.7 2,342,061 - -1.0 2,342,277 + RPSI07_RS18450 0.38 -1.0 2,342,391 + RPSI07_RS18450 0.60 -2.6 2,342,505 + RPSI07_RS18450 0.82 -3.0 2,342,506 - RPSI07_RS18450 0.82 +1.0 2,342,506 - +1.0 2,342,560 + -0.8 2,342,560 + -0.3 2,342,560 + -1.4 2,342,561 - -0.8 2,342,562 + -1.6 2,342,582 - -0.1 2,342,628 - -0.0 2,342,629 + -0.3
Or see this region's nucleotide sequence