Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS18445

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS18440 and RPSI07_RS18445 overlap by 1 nucleotidesRPSI07_RS18445 and RPSI07_RS18450 are separated by 55 nucleotidesRPSI07_RS18450 and RPSI07_RS18455 are separated by 11 nucleotides RPSI07_RS18440: RPSI07_RS18440 - DNA mismatch repair protein MutS, at 2,338,304 to 2,340,952 _RS18440 RPSI07_RS18445: RPSI07_RS18445 - hypothetical protein, at 2,340,952 to 2,342,019 _RS18445 RPSI07_RS18450: RPSI07_RS18450 - peptidylprolyl isomerase, at 2,342,075 to 2,342,599 _RS18450 RPSI07_RS18455: RPSI07_RS18455 - cupin domain-containing protein, at 2,342,611 to 2,343,840 _RS18455 Position (kb) 2340 2341 2342 2343Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2339.998 kb on - strand, within RPSI07_RS18440at 2340.045 kb on + strand, within RPSI07_RS18440at 2340.205 kb on + strand, within RPSI07_RS18440at 2340.252 kb on - strand, within RPSI07_RS18440at 2340.417 kb on + strand, within RPSI07_RS18440at 2340.418 kb on - strand, within RPSI07_RS18440at 2340.659 kb on + strand, within RPSI07_RS18440at 2340.660 kb on - strand, within RPSI07_RS18440at 2340.660 kb on - strand, within RPSI07_RS18440at 2341.282 kb on + strand, within RPSI07_RS18445at 2341.282 kb on + strand, within RPSI07_RS18445at 2341.282 kb on + strandat 2341.283 kb on - strand, within RPSI07_RS18445at 2341.283 kb on - strand, within RPSI07_RS18445at 2341.283 kb on - strand, within RPSI07_RS18445at 2341.283 kb on - strand, within RPSI07_RS18445at 2341.283 kb on - strand, within RPSI07_RS18445at 2341.454 kb on - strand, within RPSI07_RS18445at 2341.528 kb on + strand, within RPSI07_RS18445at 2341.528 kb on + strand, within RPSI07_RS18445at 2341.529 kb on - strand, within RPSI07_RS18445at 2341.742 kb on - strand, within RPSI07_RS18445at 2341.797 kb on - strand, within RPSI07_RS18445at 2342.053 kb on + strandat 2342.053 kb on + strandat 2342.061 kb on - strandat 2342.277 kb on + strand, within RPSI07_RS18450at 2342.391 kb on + strand, within RPSI07_RS18450at 2342.505 kb on + strand, within RPSI07_RS18450at 2342.506 kb on - strand, within RPSI07_RS18450at 2342.506 kb on - strandat 2342.560 kb on + strandat 2342.560 kb on + strandat 2342.560 kb on + strandat 2342.561 kb on - strandat 2342.562 kb on + strandat 2342.582 kb on - strandat 2342.628 kb on - strandat 2342.629 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
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2,339,998 - RPSI07_RS18440 0.64 +0.4
2,340,045 + RPSI07_RS18440 0.66 -0.2
2,340,205 + RPSI07_RS18440 0.72 +0.3
2,340,252 - RPSI07_RS18440 0.74 -0.0
2,340,417 + RPSI07_RS18440 0.80 +0.1
2,340,418 - RPSI07_RS18440 0.80 +2.0
2,340,659 + RPSI07_RS18440 0.89 +0.4
2,340,660 - RPSI07_RS18440 0.89 -0.7
2,340,660 - RPSI07_RS18440 0.89 +0.2
2,341,282 + RPSI07_RS18445 0.31 -0.8
2,341,282 + RPSI07_RS18445 0.31 -1.0
2,341,282 + +1.0
2,341,283 - RPSI07_RS18445 0.31 -0.6
2,341,283 - RPSI07_RS18445 0.31 +0.3
2,341,283 - RPSI07_RS18445 0.31 -4.3
2,341,283 - RPSI07_RS18445 0.31 -1.3
2,341,283 - RPSI07_RS18445 0.31 +2.6
2,341,454 - RPSI07_RS18445 0.47 -0.9
2,341,528 + RPSI07_RS18445 0.54 +1.0
2,341,528 + RPSI07_RS18445 0.54 -0.2
2,341,529 - RPSI07_RS18445 0.54 -0.3
2,341,742 - RPSI07_RS18445 0.74 +1.1
2,341,797 - RPSI07_RS18445 0.79 +0.4
2,342,053 + +0.6
2,342,053 + -1.7
2,342,061 - -1.0
2,342,277 + RPSI07_RS18450 0.38 -1.0
2,342,391 + RPSI07_RS18450 0.60 -2.6
2,342,505 + RPSI07_RS18450 0.82 -3.0
2,342,506 - RPSI07_RS18450 0.82 +1.0
2,342,506 - +1.0
2,342,560 + -0.8
2,342,560 + -0.3
2,342,560 + -1.4
2,342,561 - -0.8
2,342,562 + -1.6
2,342,582 - -0.1
2,342,628 - -0.0
2,342,629 + -0.3

Or see this region's nucleotide sequence