Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS16665 and RPSI07_RS16670 overlap by 4 nucleotides RPSI07_RS16670 and RPSI07_RS16675 are separated by 287 nucleotides RPSI07_RS16675 and RPSI07_RS16680 are separated by 7 nucleotides RPSI07_RS16680 and RPSI07_RS16685 are separated by 71 nucleotides
RPSI07_RS16665: RPSI07_RS16665 - thiazolinyl imide reductase, at 1,942,702 to 1,943,865
_RS16665
RPSI07_RS16670: RPSI07_RS16670 - peptide biosynthesis thioesterase, at 1,943,862 to 1,945,589
_RS16670
RPSI07_RS16675: RPSI07_RS16675 - hypothetical protein, at 1,945,877 to 1,946,224
_RS16675
RPSI07_RS16680: RPSI07_RS16680 - DUF1653 domain-containing protein, at 1,946,232 to 1,946,441
_RS16680
RPSI07_RS16685: RPSI07_RS16685 - hypothetical protein, at 1,946,513 to 1,947,037
_RS16685
Position (kb)
1943
1944
1945
1946 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1943.030 kb on + strand, within RPSI07_RS16665 at 1943.030 kb on + strand, within RPSI07_RS16665 at 1943.030 kb on + strand, within RPSI07_RS16665 at 1943.030 kb on + strand, within RPSI07_RS16665 at 1943.030 kb on + strand, within RPSI07_RS16665 at 1943.031 kb on - strand, within RPSI07_RS16665 at 1943.031 kb on - strand, within RPSI07_RS16665 at 1943.031 kb on - strand, within RPSI07_RS16665 at 1943.031 kb on - strand, within RPSI07_RS16665 at 1943.031 kb on - strand, within RPSI07_RS16665 at 1943.122 kb on + strand, within RPSI07_RS16665 at 1943.158 kb on + strand, within RPSI07_RS16665 at 1943.158 kb on + strand, within RPSI07_RS16665 at 1943.227 kb on + strand, within RPSI07_RS16665 at 1943.369 kb on + strand, within RPSI07_RS16665 at 1943.369 kb on + strand, within RPSI07_RS16665 at 1943.370 kb on - strand, within RPSI07_RS16665 at 1943.370 kb on - strand, within RPSI07_RS16665 at 1943.396 kb on - strand, within RPSI07_RS16665 at 1943.488 kb on + strand, within RPSI07_RS16665 at 1943.518 kb on + strand, within RPSI07_RS16665 at 1943.518 kb on + strand, within RPSI07_RS16665 at 1943.558 kb on - strand, within RPSI07_RS16665 at 1943.558 kb on - strand, within RPSI07_RS16665 at 1943.846 kb on - strand at 1943.846 kb on - strand at 1943.882 kb on + strand at 1943.883 kb on - strand at 1943.887 kb on + strand at 1943.887 kb on + strand at 1943.887 kb on + strand at 1943.887 kb on + strand at 1943.888 kb on - strand at 1943.888 kb on - strand at 1943.888 kb on - strand at 1943.953 kb on - strand at 1944.007 kb on - strand at 1944.007 kb on - strand at 1944.007 kb on - strand at 1944.633 kb on + strand, within RPSI07_RS16670 at 1944.634 kb on - strand, within RPSI07_RS16670 at 1944.634 kb on - strand, within RPSI07_RS16670 at 1945.086 kb on - strand, within RPSI07_RS16670 at 1945.286 kb on + strand, within RPSI07_RS16670 at 1945.371 kb on + strand, within RPSI07_RS16670 at 1945.371 kb on + strand, within RPSI07_RS16670 at 1945.371 kb on + strand, within RPSI07_RS16670 at 1945.371 kb on + strand, within RPSI07_RS16670 at 1945.372 kb on - strand, within RPSI07_RS16670 at 1945.372 kb on - strand, within RPSI07_RS16670 at 1945.834 kb on - strand at 1946.126 kb on + strand, within RPSI07_RS16675 at 1946.126 kb on + strand, within RPSI07_RS16675 at 1946.127 kb on - strand, within RPSI07_RS16675 at 1946.127 kb on - strand, within RPSI07_RS16675 at 1946.189 kb on + strand, within RPSI07_RS16675 at 1946.265 kb on + strand, within RPSI07_RS16680 at 1946.265 kb on + strand, within RPSI07_RS16680 at 1946.265 kb on + strand, within RPSI07_RS16680 at 1946.266 kb on - strand, within RPSI07_RS16680 at 1946.304 kb on + strand, within RPSI07_RS16680 at 1946.305 kb on - strand, within RPSI07_RS16680 at 1946.305 kb on - strand, within RPSI07_RS16680 at 1946.305 kb on - strand, within RPSI07_RS16680 at 1946.338 kb on - strand, within RPSI07_RS16680
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 1,943,030 + RPSI07_RS16665 0.28 -1.3 1,943,030 + RPSI07_RS16665 0.28 -0.7 1,943,030 + RPSI07_RS16665 0.28 -1.3 1,943,030 + RPSI07_RS16665 0.28 -1.1 1,943,030 + RPSI07_RS16665 0.28 +1.0 1,943,031 - RPSI07_RS16665 0.28 +3.0 1,943,031 - RPSI07_RS16665 0.28 -0.3 1,943,031 - RPSI07_RS16665 0.28 -0.0 1,943,031 - RPSI07_RS16665 0.28 +4.0 1,943,031 - RPSI07_RS16665 0.28 +0.1 1,943,122 + RPSI07_RS16665 0.36 +1.0 1,943,158 + RPSI07_RS16665 0.39 -0.5 1,943,158 + RPSI07_RS16665 0.39 +0.8 1,943,227 + RPSI07_RS16665 0.45 -0.3 1,943,369 + RPSI07_RS16665 0.57 +0.2 1,943,369 + RPSI07_RS16665 0.57 +0.3 1,943,370 - RPSI07_RS16665 0.57 +0.2 1,943,370 - RPSI07_RS16665 0.57 +1.4 1,943,396 - RPSI07_RS16665 0.60 +1.0 1,943,488 + RPSI07_RS16665 0.68 -0.2 1,943,518 + RPSI07_RS16665 0.70 -2.0 1,943,518 + RPSI07_RS16665 0.70 +1.1 1,943,558 - RPSI07_RS16665 0.74 +2.0 1,943,558 - RPSI07_RS16665 0.74 -0.3 1,943,846 - -0.6 1,943,846 - +1.6 1,943,882 + +0.5 1,943,883 - -0.2 1,943,887 + -2.8 1,943,887 + -0.5 1,943,887 + -0.0 1,943,887 + -0.0 1,943,888 - -1.1 1,943,888 - +2.0 1,943,888 - -0.4 1,943,953 - -0.9 1,944,007 - -1.2 1,944,007 - -0.0 1,944,007 - -0.0 1,944,633 + RPSI07_RS16670 0.45 +2.9 1,944,634 - RPSI07_RS16670 0.45 +1.2 1,944,634 - RPSI07_RS16670 0.45 +2.6 1,945,086 - RPSI07_RS16670 0.71 -0.3 1,945,286 + RPSI07_RS16670 0.82 -0.9 1,945,371 + RPSI07_RS16670 0.87 +2.0 1,945,371 + RPSI07_RS16670 0.87 +0.4 1,945,371 + RPSI07_RS16670 0.87 +1.9 1,945,371 + RPSI07_RS16670 0.87 -0.2 1,945,372 - RPSI07_RS16670 0.87 -0.5 1,945,372 - RPSI07_RS16670 0.87 -2.1 1,945,834 - -1.2 1,946,126 + RPSI07_RS16675 0.72 +1.3 1,946,126 + RPSI07_RS16675 0.72 -0.9 1,946,127 - RPSI07_RS16675 0.72 +0.4 1,946,127 - RPSI07_RS16675 0.72 -0.8 1,946,189 + RPSI07_RS16675 0.90 +3.0 1,946,265 + RPSI07_RS16680 0.16 -0.5 1,946,265 + RPSI07_RS16680 0.16 -0.6 1,946,265 + RPSI07_RS16680 0.16 -0.8 1,946,266 - RPSI07_RS16680 0.16 -1.1 1,946,304 + RPSI07_RS16680 0.34 -1.3 1,946,305 - RPSI07_RS16680 0.35 -0.1 1,946,305 - RPSI07_RS16680 0.35 +0.3 1,946,305 - RPSI07_RS16680 0.35 +0.2 1,946,338 - RPSI07_RS16680 0.50 +0.3
Or see this region's nucleotide sequence