Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS16600 and RPSI07_RS16605 are separated by 594 nucleotides RPSI07_RS16605 and RPSI07_RS25130 are separated by 261 nucleotides RPSI07_RS25130 and RPSI07_RS16615 are separated by 134 nucleotides
RPSI07_RS16600: RPSI07_RS16600 - integrase, at 1,900,221 to 1,900,712
_RS16600
RPSI07_RS16605: RPSI07_RS16605 - FAD-dependent oxidoreductase, at 1,901,307 to 1,903,901
_RS16605
RPSI07_RS25130: RPSI07_RS25130 - integrase, at 1,904,163 to 1,904,559
_RS25130
RPSI07_RS16615: RPSI07_RS16615 - transposase, at 1,904,694 to 1,906,169
_RS16615
Position (kb)
1901
1902
1903
1904 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1900.366 kb on + strand, within RPSI07_RS16600 at 1900.366 kb on + strand, within RPSI07_RS16600 at 1900.367 kb on - strand, within RPSI07_RS16600 at 1900.367 kb on - strand, within RPSI07_RS16600 at 1900.368 kb on - strand, within RPSI07_RS16600 at 1900.401 kb on - strand, within RPSI07_RS16600 at 1900.401 kb on - strand, within RPSI07_RS16600 at 1900.731 kb on + strand at 1900.731 kb on + strand at 1900.731 kb on + strand at 1900.732 kb on - strand at 1900.732 kb on - strand at 1900.732 kb on - strand at 1900.732 kb on - strand at 1900.770 kb on - strand at 1900.770 kb on - strand at 1900.771 kb on + strand at 1900.771 kb on + strand at 1900.772 kb on - strand at 1900.845 kb on + strand at 1900.845 kb on + strand at 1900.845 kb on + strand at 1900.845 kb on + strand at 1900.846 kb on - strand at 1900.846 kb on - strand at 1900.846 kb on - strand at 1901.473 kb on + strand at 1901.545 kb on - strand at 1901.604 kb on - strand, within RPSI07_RS16605 at 1901.806 kb on - strand, within RPSI07_RS16605 at 1901.818 kb on - strand, within RPSI07_RS16605 at 1901.972 kb on - strand, within RPSI07_RS16605 at 1902.086 kb on + strand, within RPSI07_RS16605 at 1902.087 kb on - strand, within RPSI07_RS16605 at 1902.246 kb on + strand, within RPSI07_RS16605 at 1902.246 kb on + strand, within RPSI07_RS16605 at 1902.246 kb on + strand, within RPSI07_RS16605 at 1902.247 kb on - strand, within RPSI07_RS16605 at 1902.247 kb on - strand, within RPSI07_RS16605 at 1902.247 kb on - strand, within RPSI07_RS16605 at 1902.252 kb on - strand, within RPSI07_RS16605 at 1902.252 kb on - strand, within RPSI07_RS16605 at 1902.330 kb on + strand, within RPSI07_RS16605 at 1902.330 kb on + strand, within RPSI07_RS16605 at 1902.330 kb on + strand, within RPSI07_RS16605 at 1902.330 kb on + strand, within RPSI07_RS16605 at 1902.330 kb on + strand, within RPSI07_RS16605 at 1902.331 kb on - strand, within RPSI07_RS16605 at 1902.331 kb on - strand, within RPSI07_RS16605 at 1902.331 kb on - strand, within RPSI07_RS16605 at 1902.331 kb on - strand, within RPSI07_RS16605 at 1902.331 kb on - strand, within RPSI07_RS16605 at 1902.353 kb on + strand, within RPSI07_RS16605 at 1902.353 kb on + strand, within RPSI07_RS16605 at 1902.353 kb on + strand, within RPSI07_RS16605 at 1902.354 kb on - strand, within RPSI07_RS16605 at 1902.393 kb on - strand, within RPSI07_RS16605 at 1902.393 kb on - strand, within RPSI07_RS16605 at 1902.393 kb on - strand, within RPSI07_RS16605 at 1902.449 kb on + strand, within RPSI07_RS16605 at 1902.450 kb on - strand, within RPSI07_RS16605 at 1902.450 kb on - strand, within RPSI07_RS16605 at 1902.554 kb on + strand, within RPSI07_RS16605 at 1902.594 kb on - strand, within RPSI07_RS16605 at 1902.784 kb on + strand, within RPSI07_RS16605 at 1902.785 kb on - strand, within RPSI07_RS16605 at 1902.791 kb on + strand, within RPSI07_RS16605 at 1902.792 kb on - strand, within RPSI07_RS16605 at 1902.792 kb on - strand, within RPSI07_RS16605 at 1902.792 kb on - strand, within RPSI07_RS16605 at 1902.792 kb on - strand, within RPSI07_RS16605 at 1902.810 kb on - strand, within RPSI07_RS16605 at 1902.810 kb on - strand, within RPSI07_RS16605 at 1902.993 kb on + strand, within RPSI07_RS16605 at 1902.993 kb on + strand, within RPSI07_RS16605 at 1903.071 kb on - strand, within RPSI07_RS16605 at 1903.244 kb on - strand, within RPSI07_RS16605 at 1903.252 kb on - strand, within RPSI07_RS16605 at 1903.373 kb on - strand, within RPSI07_RS16605 at 1903.549 kb on - strand, within RPSI07_RS16605 at 1903.615 kb on + strand, within RPSI07_RS16605 at 1903.615 kb on + strand, within RPSI07_RS16605 at 1903.615 kb on + strand, within RPSI07_RS16605 at 1903.615 kb on + strand, within RPSI07_RS16605 at 1903.899 kb on + strand at 1903.900 kb on - strand at 1903.929 kb on - strand at 1903.943 kb on + strand at 1903.943 kb on + strand at 1903.943 kb on + strand at 1903.954 kb on + strand at 1903.954 kb on + strand at 1903.955 kb on - strand at 1903.955 kb on - strand at 1903.955 kb on - strand at 1904.059 kb on + strand at 1904.059 kb on + strand at 1904.059 kb on + strand at 1904.059 kb on + strand at 1904.059 kb on + strand at 1904.060 kb on - strand at 1904.060 kb on - strand at 1904.060 kb on - strand at 1904.060 kb on - strand at 1904.060 kb on - strand at 1904.060 kb on - strand at 1904.068 kb on + strand at 1904.068 kb on + strand at 1904.068 kb on + strand at 1904.069 kb on - strand at 1904.125 kb on - strand at 1904.125 kb on - strand at 1904.135 kb on - strand at 1904.182 kb on + strand at 1904.183 kb on - strand at 1904.183 kb on - strand at 1904.183 kb on - strand at 1904.215 kb on + strand, within RPSI07_RS25130 at 1904.242 kb on + strand, within RPSI07_RS25130 at 1904.508 kb on + strand, within RPSI07_RS25130 at 1904.508 kb on + strand, within RPSI07_RS25130 at 1904.508 kb on + strand, within RPSI07_RS25130 at 1904.508 kb on + strand, within RPSI07_RS25130 at 1904.508 kb on + strand, within RPSI07_RS25130 at 1904.508 kb on + strand, within RPSI07_RS25130 at 1904.508 kb on + strand, within RPSI07_RS25130 at 1904.509 kb on - strand, within RPSI07_RS25130 at 1904.581 kb on - strand at 1904.581 kb on - strand at 1904.612 kb on - strand at 1904.612 kb on - strand at 1904.632 kb on + strand at 1904.632 kb on + strand at 1904.632 kb on + strand at 1904.633 kb on - strand at 1904.633 kb on - strand at 1904.633 kb on - strand at 1904.640 kb on - strand at 1904.640 kb on - strand at 1904.640 kb on - strand at 1904.640 kb on - strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.650 kb on + strand at 1904.651 kb on - strand at 1904.651 kb on - strand at 1904.651 kb on - strand at 1904.651 kb on - strand at 1904.651 kb on - strand at 1904.651 kb on - strand at 1904.651 kb on - strand at 1904.651 kb on - strand at 1904.651 kb on - strand at 1904.651 kb on - strand at 1904.651 kb on - strand at 1904.651 kb on - strand at 1904.651 kb on - strand at 1904.651 kb on - strand at 1904.652 kb on - strand at 1904.659 kb on + strand at 1904.659 kb on + strand at 1904.660 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 1,900,366 + RPSI07_RS16600 0.29 -0.3 1,900,366 + RPSI07_RS16600 0.29 -1.2 1,900,367 - RPSI07_RS16600 0.30 +0.5 1,900,367 - RPSI07_RS16600 0.30 +2.1 1,900,368 - RPSI07_RS16600 0.30 +0.0 1,900,401 - RPSI07_RS16600 0.37 -3.7 1,900,401 - RPSI07_RS16600 0.37 +0.1 1,900,731 + -1.8 1,900,731 + +2.0 1,900,731 + +0.2 1,900,732 - -0.3 1,900,732 - -1.8 1,900,732 - +1.5 1,900,732 - -0.0 1,900,770 - -0.8 1,900,770 - -0.4 1,900,771 + -2.3 1,900,771 + -0.2 1,900,772 - -0.6 1,900,845 + -1.1 1,900,845 + -0.6 1,900,845 + +0.6 1,900,845 + +0.7 1,900,846 - +1.0 1,900,846 - -1.5 1,900,846 - -0.0 1,901,473 + -0.5 1,901,545 - -0.4 1,901,604 - RPSI07_RS16605 0.11 -1.1 1,901,806 - RPSI07_RS16605 0.19 +0.8 1,901,818 - RPSI07_RS16605 0.20 -0.7 1,901,972 - RPSI07_RS16605 0.26 -0.3 1,902,086 + RPSI07_RS16605 0.30 +1.0 1,902,087 - RPSI07_RS16605 0.30 -0.9 1,902,246 + RPSI07_RS16605 0.36 -0.4 1,902,246 + RPSI07_RS16605 0.36 +0.4 1,902,246 + RPSI07_RS16605 0.36 +0.5 1,902,247 - RPSI07_RS16605 0.36 -1.6 1,902,247 - RPSI07_RS16605 0.36 +0.9 1,902,247 - RPSI07_RS16605 0.36 -0.3 1,902,252 - RPSI07_RS16605 0.36 -0.6 1,902,252 - RPSI07_RS16605 0.36 -0.6 1,902,330 + RPSI07_RS16605 0.39 -0.4 1,902,330 + RPSI07_RS16605 0.39 +0.4 1,902,330 + RPSI07_RS16605 0.39 +0.1 1,902,330 + RPSI07_RS16605 0.39 +0.3 1,902,330 + RPSI07_RS16605 0.39 +1.4 1,902,331 - RPSI07_RS16605 0.39 -0.7 1,902,331 - RPSI07_RS16605 0.39 -1.2 1,902,331 - RPSI07_RS16605 0.39 -0.3 1,902,331 - RPSI07_RS16605 0.39 +0.4 1,902,331 - RPSI07_RS16605 0.39 +0.6 1,902,353 + RPSI07_RS16605 0.40 -2.6 1,902,353 + RPSI07_RS16605 0.40 -0.0 1,902,353 + RPSI07_RS16605 0.40 -0.9 1,902,354 - RPSI07_RS16605 0.40 -0.0 1,902,393 - RPSI07_RS16605 0.42 -0.3 1,902,393 - RPSI07_RS16605 0.42 +0.2 1,902,393 - RPSI07_RS16605 0.42 -1.9 1,902,449 + RPSI07_RS16605 0.44 -0.4 1,902,450 - RPSI07_RS16605 0.44 +0.8 1,902,450 - RPSI07_RS16605 0.44 +1.4 1,902,554 + RPSI07_RS16605 0.48 +0.7 1,902,594 - RPSI07_RS16605 0.50 -1.3 1,902,784 + RPSI07_RS16605 0.57 -0.6 1,902,785 - RPSI07_RS16605 0.57 -0.0 1,902,791 + RPSI07_RS16605 0.57 +1.0 1,902,792 - RPSI07_RS16605 0.57 -0.3 1,902,792 - RPSI07_RS16605 0.57 -0.3 1,902,792 - RPSI07_RS16605 0.57 -0.9 1,902,792 - RPSI07_RS16605 0.57 +1.1 1,902,810 - RPSI07_RS16605 0.58 -0.8 1,902,810 - RPSI07_RS16605 0.58 -1.2 1,902,993 + RPSI07_RS16605 0.65 -0.0 1,902,993 + RPSI07_RS16605 0.65 +3.8 1,903,071 - RPSI07_RS16605 0.68 -0.1 1,903,244 - RPSI07_RS16605 0.75 -0.0 1,903,252 - RPSI07_RS16605 0.75 +0.2 1,903,373 - RPSI07_RS16605 0.80 +0.4 1,903,549 - RPSI07_RS16605 0.86 -0.6 1,903,615 + RPSI07_RS16605 0.89 +2.0 1,903,615 + RPSI07_RS16605 0.89 -0.1 1,903,615 + RPSI07_RS16605 0.89 +1.2 1,903,615 + RPSI07_RS16605 0.89 -0.3 1,903,899 + +1.0 1,903,900 - -0.5 1,903,929 - -1.3 1,903,943 + +1.0 1,903,943 + +3.0 1,903,943 + -0.2 1,903,954 + -1.0 1,903,954 + +0.7 1,903,955 - -0.1 1,903,955 - -0.0 1,903,955 - -0.8 1,904,059 + -1.0 1,904,059 + -1.2 1,904,059 + +1.0 1,904,059 + +0.7 1,904,059 + -1.0 1,904,060 - -2.2 1,904,060 - -1.6 1,904,060 - +1.2 1,904,060 - +1.8 1,904,060 - -0.6 1,904,060 - +1.1 1,904,068 + +1.0 1,904,068 + +1.7 1,904,068 + +0.1 1,904,069 - +0.2 1,904,125 - +0.5 1,904,125 - -2.1 1,904,135 - -0.2 1,904,182 + -1.9 1,904,183 - -0.6 1,904,183 - +0.6 1,904,183 - -1.2 1,904,215 + RPSI07_RS25130 0.13 -0.1 1,904,242 + RPSI07_RS25130 0.20 +2.2 1,904,508 + RPSI07_RS25130 0.87 -2.3 1,904,508 + RPSI07_RS25130 0.87 -1.2 1,904,508 + RPSI07_RS25130 0.87 -3.5 1,904,508 + RPSI07_RS25130 0.87 -0.6 1,904,508 + RPSI07_RS25130 0.87 -1.1 1,904,508 + RPSI07_RS25130 0.87 -3.2 1,904,508 + RPSI07_RS25130 0.87 +1.0 1,904,509 - RPSI07_RS25130 0.87 -1.4 1,904,581 - +0.0 1,904,581 - +3.3 1,904,612 - -0.7 1,904,612 - -1.1 1,904,632 + +0.4 1,904,632 + +0.2 1,904,632 + +0.0 1,904,633 - -0.4 1,904,633 - -0.1 1,904,633 - +0.4 1,904,640 - +0.6 1,904,640 - +0.1 1,904,640 - -1.7 1,904,640 - -0.0 1,904,650 + +0.0 1,904,650 + +0.4 1,904,650 + -1.7 1,904,650 + +0.6 1,904,650 + +0.8 1,904,650 + -0.5 1,904,650 + -0.0 1,904,650 + +2.0 1,904,650 + -0.6 1,904,650 + +0.0 1,904,650 + +0.3 1,904,650 + +2.3 1,904,650 + +1.0 1,904,650 + -0.7 1,904,650 + -0.6 1,904,650 + -0.4 1,904,650 + -0.0 1,904,650 + +0.4 1,904,650 + +1.0 1,904,650 + -0.4 1,904,650 + -0.2 1,904,650 + +0.2 1,904,650 + -1.6 1,904,650 + -0.1 1,904,650 + -1.0 1,904,650 + -0.8 1,904,650 + -0.7 1,904,650 + -0.7 1,904,650 + +2.6 1,904,650 + -0.2 1,904,650 + -0.4 1,904,650 + -0.5 1,904,650 + +2.4 1,904,650 + -1.0 1,904,650 + +0.1 1,904,651 - -0.7 1,904,651 - +2.6 1,904,651 - -0.4 1,904,651 - -0.8 1,904,651 - -0.7 1,904,651 - -1.6 1,904,651 - -0.5 1,904,651 - -1.0 1,904,651 - +1.0 1,904,651 - -0.5 1,904,651 - -0.3 1,904,651 - -0.7 1,904,651 - +1.6 1,904,651 - -0.4 1,904,652 - +0.3 1,904,659 + +2.6 1,904,659 + +2.1 1,904,660 - -0.1
Or see this region's nucleotide sequence