Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS16590 and RPSI07_RS16595 are separated by 108 nucleotides RPSI07_RS16595 and RPSI07_RS16600 are separated by 34 nucleotides RPSI07_RS16600 and RPSI07_RS16605 are separated by 594 nucleotides
RPSI07_RS16590: RPSI07_RS16590 - cytochrome C, at 1,898,588 to 1,899,790
_RS16590
RPSI07_RS16595: RPSI07_RS16595 - iron oxidase, at 1,899,899 to 1,900,186
_RS16595
RPSI07_RS16600: RPSI07_RS16600 - integrase, at 1,900,221 to 1,900,712
_RS16600
RPSI07_RS16605: RPSI07_RS16605 - FAD-dependent oxidoreductase, at 1,901,307 to 1,903,901
_RS16605
Position (kb)
1900
1901 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1899.227 kb on + strand, within RPSI07_RS16590 at 1899.227 kb on + strand, within RPSI07_RS16590 at 1899.227 kb on + strand, within RPSI07_RS16590 at 1899.227 kb on + strand, within RPSI07_RS16590 at 1899.227 kb on + strand, within RPSI07_RS16590 at 1899.227 kb on + strand, within RPSI07_RS16590 at 1899.228 kb on - strand, within RPSI07_RS16590 at 1899.228 kb on - strand, within RPSI07_RS16590 at 1899.228 kb on - strand, within RPSI07_RS16590 at 1899.356 kb on + strand, within RPSI07_RS16590 at 1899.356 kb on + strand, within RPSI07_RS16590 at 1899.356 kb on + strand, within RPSI07_RS16590 at 1899.357 kb on - strand, within RPSI07_RS16590 at 1899.401 kb on + strand, within RPSI07_RS16590 at 1899.401 kb on + strand, within RPSI07_RS16590 at 1899.402 kb on - strand, within RPSI07_RS16590 at 1899.402 kb on - strand, within RPSI07_RS16590 at 1899.497 kb on + strand, within RPSI07_RS16590 at 1899.498 kb on - strand, within RPSI07_RS16590 at 1899.498 kb on - strand, within RPSI07_RS16590 at 1899.498 kb on - strand, within RPSI07_RS16590 at 1899.503 kb on + strand, within RPSI07_RS16590 at 1899.503 kb on + strand, within RPSI07_RS16590 at 1899.504 kb on + strand, within RPSI07_RS16590 at 1899.504 kb on - strand, within RPSI07_RS16590 at 1899.511 kb on + strand, within RPSI07_RS16590 at 1899.511 kb on + strand, within RPSI07_RS16590 at 1899.511 kb on + strand, within RPSI07_RS16590 at 1899.511 kb on + strand, within RPSI07_RS16590 at 1899.511 kb on + strand, within RPSI07_RS16590 at 1899.511 kb on + strand, within RPSI07_RS16590 at 1899.512 kb on - strand, within RPSI07_RS16590 at 1899.512 kb on - strand, within RPSI07_RS16590 at 1899.512 kb on - strand, within RPSI07_RS16590 at 1899.512 kb on - strand, within RPSI07_RS16590 at 1899.512 kb on - strand, within RPSI07_RS16590 at 1899.512 kb on - strand, within RPSI07_RS16590 at 1899.516 kb on - strand, within RPSI07_RS16590 at 1899.516 kb on - strand, within RPSI07_RS16590 at 1899.595 kb on + strand, within RPSI07_RS16590 at 1899.605 kb on + strand, within RPSI07_RS16590 at 1899.605 kb on + strand, within RPSI07_RS16590 at 1899.606 kb on - strand, within RPSI07_RS16590 at 1899.606 kb on - strand, within RPSI07_RS16590 at 1899.606 kb on - strand, within RPSI07_RS16590 at 1899.623 kb on + strand, within RPSI07_RS16590 at 1899.623 kb on + strand, within RPSI07_RS16590 at 1899.683 kb on + strand at 1899.683 kb on + strand at 1899.684 kb on - strand at 1899.692 kb on + strand at 1899.692 kb on + strand at 1899.693 kb on - strand at 1899.771 kb on - strand at 1899.781 kb on + strand at 1899.782 kb on - strand at 1899.782 kb on - strand at 1899.789 kb on - strand at 1899.789 kb on - strand at 1899.789 kb on - strand at 1899.815 kb on + strand at 1900.185 kb on - strand at 1900.366 kb on + strand, within RPSI07_RS16600 at 1900.366 kb on + strand, within RPSI07_RS16600 at 1900.367 kb on - strand, within RPSI07_RS16600 at 1900.367 kb on - strand, within RPSI07_RS16600 at 1900.368 kb on - strand, within RPSI07_RS16600 at 1900.401 kb on - strand, within RPSI07_RS16600 at 1900.401 kb on - strand, within RPSI07_RS16600 at 1900.731 kb on + strand at 1900.731 kb on + strand at 1900.731 kb on + strand at 1900.732 kb on - strand at 1900.732 kb on - strand at 1900.732 kb on - strand at 1900.732 kb on - strand at 1900.770 kb on - strand at 1900.770 kb on - strand at 1900.771 kb on + strand at 1900.771 kb on + strand at 1900.772 kb on - strand at 1900.845 kb on + strand at 1900.845 kb on + strand at 1900.845 kb on + strand at 1900.845 kb on + strand at 1900.846 kb on - strand at 1900.846 kb on - strand at 1900.846 kb on - strand at 1901.473 kb on + strand at 1901.545 kb on - strand at 1901.604 kb on - strand, within RPSI07_RS16605
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 1,899,227 + RPSI07_RS16590 0.53 -0.0 1,899,227 + RPSI07_RS16590 0.53 +0.1 1,899,227 + RPSI07_RS16590 0.53 -0.8 1,899,227 + RPSI07_RS16590 0.53 -1.8 1,899,227 + RPSI07_RS16590 0.53 -0.2 1,899,227 + RPSI07_RS16590 0.53 -0.8 1,899,228 - RPSI07_RS16590 0.53 -0.5 1,899,228 - RPSI07_RS16590 0.53 -1.5 1,899,228 - RPSI07_RS16590 0.53 -0.3 1,899,356 + RPSI07_RS16590 0.64 +1.0 1,899,356 + RPSI07_RS16590 0.64 -0.2 1,899,356 + RPSI07_RS16590 0.64 +0.1 1,899,357 - RPSI07_RS16590 0.64 -0.8 1,899,401 + RPSI07_RS16590 0.68 -1.3 1,899,401 + RPSI07_RS16590 0.68 -1.0 1,899,402 - RPSI07_RS16590 0.68 +0.8 1,899,402 - RPSI07_RS16590 0.68 +2.8 1,899,497 + RPSI07_RS16590 0.76 +1.6 1,899,498 - RPSI07_RS16590 0.76 -1.3 1,899,498 - RPSI07_RS16590 0.76 +0.2 1,899,498 - RPSI07_RS16590 0.76 +3.3 1,899,503 + RPSI07_RS16590 0.76 +1.4 1,899,503 + RPSI07_RS16590 0.76 +1.3 1,899,504 + RPSI07_RS16590 0.76 -1.1 1,899,504 - RPSI07_RS16590 0.76 +1.4 1,899,511 + RPSI07_RS16590 0.77 +2.0 1,899,511 + RPSI07_RS16590 0.77 -0.8 1,899,511 + RPSI07_RS16590 0.77 +1.0 1,899,511 + RPSI07_RS16590 0.77 -1.1 1,899,511 + RPSI07_RS16590 0.77 +0.6 1,899,511 + RPSI07_RS16590 0.77 +2.3 1,899,512 - RPSI07_RS16590 0.77 -0.3 1,899,512 - RPSI07_RS16590 0.77 +1.0 1,899,512 - RPSI07_RS16590 0.77 +0.3 1,899,512 - RPSI07_RS16590 0.77 -0.6 1,899,512 - RPSI07_RS16590 0.77 +0.7 1,899,512 - RPSI07_RS16590 0.77 +3.0 1,899,516 - RPSI07_RS16590 0.77 +0.7 1,899,516 - RPSI07_RS16590 0.77 +1.5 1,899,595 + RPSI07_RS16590 0.84 +0.7 1,899,605 + RPSI07_RS16590 0.85 +1.4 1,899,605 + RPSI07_RS16590 0.85 -1.7 1,899,606 - RPSI07_RS16590 0.85 +2.0 1,899,606 - RPSI07_RS16590 0.85 -0.1 1,899,606 - RPSI07_RS16590 0.85 -0.2 1,899,623 + RPSI07_RS16590 0.86 -0.5 1,899,623 + RPSI07_RS16590 0.86 +3.4 1,899,683 + -0.8 1,899,683 + -0.8 1,899,684 - -1.4 1,899,692 + +2.6 1,899,692 + -0.8 1,899,693 - +0.7 1,899,771 - +1.1 1,899,781 + +2.0 1,899,782 - -1.3 1,899,782 - +0.8 1,899,789 - -0.4 1,899,789 - +0.9 1,899,789 - +0.4 1,899,815 + +1.5 1,900,185 - +1.0 1,900,366 + RPSI07_RS16600 0.29 -0.3 1,900,366 + RPSI07_RS16600 0.29 -1.2 1,900,367 - RPSI07_RS16600 0.30 +0.5 1,900,367 - RPSI07_RS16600 0.30 +2.1 1,900,368 - RPSI07_RS16600 0.30 +0.0 1,900,401 - RPSI07_RS16600 0.37 -3.7 1,900,401 - RPSI07_RS16600 0.37 +0.1 1,900,731 + -1.8 1,900,731 + +2.0 1,900,731 + +0.2 1,900,732 - -0.3 1,900,732 - -1.8 1,900,732 - +1.5 1,900,732 - -0.0 1,900,770 - -0.8 1,900,770 - -0.4 1,900,771 + -2.3 1,900,771 + -0.2 1,900,772 - -0.6 1,900,845 + -1.1 1,900,845 + -0.6 1,900,845 + +0.6 1,900,845 + +0.7 1,900,846 - +1.0 1,900,846 - -1.5 1,900,846 - -0.0 1,901,473 + -0.5 1,901,545 - -0.4 1,901,604 - RPSI07_RS16605 0.11 -1.1
Or see this region's nucleotide sequence