Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS16565 and RPSI07_RS16570 are separated by 341 nucleotides RPSI07_RS16570 and RPSI07_RS16575 are separated by 477 nucleotides
RPSI07_RS16565: RPSI07_RS16565 - hypothetical protein, at 1,878,235 to 1,878,597
_RS16565
RPSI07_RS16570: RPSI07_RS16570 - Type III effector protein (Skwp 4), at 1,878,939 to 1,888,208
_RS16570
RPSI07_RS16575: RPSI07_RS16575 - type III effector protein, at 1,888,686 to 1,896,041
_RS16575
Position (kb)
1878
1879
1880
1881
1882
1883
1884
1885
1886
1887
1888
1889 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1878.076 kb on + strand at 1878.167 kb on + strand at 1878.167 kb on + strand at 1878.230 kb on + strand at 1878.337 kb on - strand, within RPSI07_RS16565 at 1878.738 kb on + strand at 1878.738 kb on + strand at 1878.739 kb on - strand at 1878.759 kb on + strand at 1878.759 kb on + strand at 1878.760 kb on - strand at 1878.760 kb on - strand at 1878.760 kb on - strand at 1878.760 kb on - strand at 1878.760 kb on - strand at 1878.763 kb on + strand at 1878.764 kb on - strand at 1878.764 kb on - strand at 1878.764 kb on - strand at 1878.764 kb on - strand at 1878.764 kb on - strand at 1878.780 kb on - strand at 1878.780 kb on - strand at 1878.780 kb on - strand at 1878.783 kb on + strand at 1878.783 kb on + strand at 1878.784 kb on - strand at 1878.784 kb on - strand at 1878.849 kb on + strand at 1878.849 kb on + strand at 1878.867 kb on - strand at 1879.007 kb on + strand at 1879.008 kb on - strand at 1879.008 kb on - strand at 1879.185 kb on + strand at 1879.185 kb on + strand at 1879.185 kb on + strand at 1879.186 kb on - strand at 1879.186 kb on - strand at 1879.186 kb on - strand at 1879.201 kb on + strand at 1879.201 kb on + strand at 1879.201 kb on + strand at 1879.201 kb on + strand at 1879.201 kb on + strand at 1879.201 kb on + strand at 1879.201 kb on + strand at 1879.201 kb on + strand at 1879.202 kb on - strand at 1879.202 kb on - strand at 1879.202 kb on - strand at 1879.202 kb on - strand at 1879.408 kb on + strand at 1879.408 kb on + strand at 1879.408 kb on + strand at 1879.409 kb on - strand at 1879.409 kb on - strand at 1879.410 kb on - strand at 1879.553 kb on + strand at 1879.553 kb on + strand at 1879.553 kb on + strand at 1879.553 kb on + strand at 1879.554 kb on - strand at 1879.554 kb on - strand at 1879.554 kb on - strand at 1879.554 kb on - strand at 1879.950 kb on + strand, within RPSI07_RS16570 at 1879.951 kb on - strand, within RPSI07_RS16570 at 1879.951 kb on - strand, within RPSI07_RS16570 at 1879.951 kb on - strand, within RPSI07_RS16570 at 1880.122 kb on + strand, within RPSI07_RS16570 at 1880.122 kb on + strand, within RPSI07_RS16570 at 1880.122 kb on + strand, within RPSI07_RS16570 at 1880.122 kb on + strand, within RPSI07_RS16570 at 1880.123 kb on - strand, within RPSI07_RS16570 at 1880.123 kb on - strand, within RPSI07_RS16570 at 1880.123 kb on - strand, within RPSI07_RS16570 at 1880.129 kb on + strand, within RPSI07_RS16570 at 1880.308 kb on + strand, within RPSI07_RS16570 at 1880.686 kb on + strand, within RPSI07_RS16570 at 1880.687 kb on - strand, within RPSI07_RS16570 at 1880.729 kb on + strand, within RPSI07_RS16570 at 1880.729 kb on + strand, within RPSI07_RS16570 at 1880.730 kb on - strand, within RPSI07_RS16570 at 1880.734 kb on - strand, within RPSI07_RS16570 at 1880.926 kb on + strand, within RPSI07_RS16570 at 1880.926 kb on + strand, within RPSI07_RS16570 at 1881.502 kb on + strand, within RPSI07_RS16570 at 1881.503 kb on - strand, within RPSI07_RS16570 at 1881.503 kb on - strand, within RPSI07_RS16570 at 1881.574 kb on + strand, within RPSI07_RS16570 at 1881.612 kb on - strand, within RPSI07_RS16570 at 1881.747 kb on + strand, within RPSI07_RS16570 at 1881.747 kb on + strand, within RPSI07_RS16570 at 1881.747 kb on + strand, within RPSI07_RS16570 at 1881.747 kb on + strand, within RPSI07_RS16570 at 1881.748 kb on - strand, within RPSI07_RS16570 at 1881.748 kb on - strand, within RPSI07_RS16570 at 1881.860 kb on - strand, within RPSI07_RS16570 at 1881.860 kb on - strand, within RPSI07_RS16570 at 1881.860 kb on - strand, within RPSI07_RS16570 at 1881.907 kb on + strand, within RPSI07_RS16570 at 1881.907 kb on + strand, within RPSI07_RS16570 at 1881.907 kb on + strand, within RPSI07_RS16570 at 1881.908 kb on - strand, within RPSI07_RS16570 at 1882.051 kb on + strand, within RPSI07_RS16570 at 1882.167 kb on - strand, within RPSI07_RS16570 at 1882.188 kb on + strand, within RPSI07_RS16570 at 1882.189 kb on - strand, within RPSI07_RS16570 at 1882.264 kb on + strand, within RPSI07_RS16570 at 1882.264 kb on + strand, within RPSI07_RS16570 at 1882.265 kb on - strand, within RPSI07_RS16570 at 1882.505 kb on - strand, within RPSI07_RS16570 at 1882.505 kb on - strand, within RPSI07_RS16570 at 1882.505 kb on - strand, within RPSI07_RS16570 at 1882.510 kb on + strand, within RPSI07_RS16570 at 1882.681 kb on + strand, within RPSI07_RS16570 at 1882.681 kb on + strand, within RPSI07_RS16570 at 1882.682 kb on - strand, within RPSI07_RS16570 at 1882.682 kb on - strand, within RPSI07_RS16570 at 1882.682 kb on - strand, within RPSI07_RS16570 at 1882.682 kb on - strand, within RPSI07_RS16570 at 1882.793 kb on + strand, within RPSI07_RS16570 at 1882.793 kb on + strand, within RPSI07_RS16570 at 1882.793 kb on + strand, within RPSI07_RS16570 at 1882.880 kb on - strand, within RPSI07_RS16570 at 1883.285 kb on - strand, within RPSI07_RS16570 at 1883.529 kb on - strand, within RPSI07_RS16570 at 1883.529 kb on - strand, within RPSI07_RS16570 at 1883.529 kb on - strand, within RPSI07_RS16570 at 1883.911 kb on + strand, within RPSI07_RS16570 at 1883.912 kb on - strand, within RPSI07_RS16570 at 1884.025 kb on + strand, within RPSI07_RS16570 at 1884.025 kb on + strand, within RPSI07_RS16570 at 1884.025 kb on + strand, within RPSI07_RS16570 at 1884.025 kb on + strand, within RPSI07_RS16570 at 1884.026 kb on - strand, within RPSI07_RS16570 at 1884.026 kb on - strand, within RPSI07_RS16570 at 1884.026 kb on - strand, within RPSI07_RS16570 at 1884.029 kb on - strand, within RPSI07_RS16570 at 1884.058 kb on - strand, within RPSI07_RS16570 at 1884.681 kb on - strand, within RPSI07_RS16570 at 1887.131 kb on - strand, within RPSI07_RS16570 at 1887.328 kb on + strand at 1887.328 kb on + strand at 1887.328 kb on + strand at 1887.329 kb on - strand at 1887.329 kb on - strand at 1887.329 kb on - strand at 1887.329 kb on - strand at 1887.364 kb on + strand at 1887.365 kb on - strand at 1887.365 kb on - strand at 1887.403 kb on + strand at 1887.403 kb on + strand at 1887.403 kb on + strand at 1887.403 kb on + strand at 1887.403 kb on + strand at 1887.491 kb on - strand at 1887.491 kb on - strand at 1887.491 kb on - strand at 1887.618 kb on + strand at 1887.618 kb on + strand at 1887.618 kb on + strand at 1887.618 kb on + strand at 1887.619 kb on - strand at 1887.761 kb on + strand at 1887.762 kb on - strand at 1887.762 kb on - strand at 1887.762 kb on - strand at 1887.821 kb on - strand at 1888.172 kb on + strand at 1888.172 kb on + strand at 1888.172 kb on + strand at 1888.172 kb on + strand at 1888.172 kb on + strand at 1888.173 kb on - strand at 1888.173 kb on - strand at 1888.209 kb on - strand at 1888.300 kb on + strand at 1888.301 kb on - strand at 1888.404 kb on + strand at 1888.405 kb on - strand at 1888.405 kb on - strand at 1888.443 kb on - strand at 1888.443 kb on - strand at 1888.443 kb on - strand at 1888.486 kb on + strand at 1888.486 kb on + strand at 1888.487 kb on - strand at 1888.514 kb on + strand at 1888.514 kb on + strand at 1888.514 kb on + strand at 1888.515 kb on - strand at 1888.515 kb on - strand at 1888.597 kb on + strand at 1888.597 kb on + strand at 1888.597 kb on + strand at 1888.598 kb on - strand at 1888.665 kb on + strand at 1888.665 kb on + strand at 1888.665 kb on + strand at 1888.666 kb on - strand at 1888.666 kb on - strand at 1888.819 kb on + strand at 1888.984 kb on + strand at 1888.985 kb on - strand at 1889.089 kb on + strand at 1889.089 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 1,878,076 + +1.3 1,878,167 + +2.6 1,878,167 + -0.6 1,878,230 + +2.0 1,878,337 - RPSI07_RS16565 0.28 -2.2 1,878,738 + +0.2 1,878,738 + +1.8 1,878,739 - +0.4 1,878,759 + -1.0 1,878,759 + +0.6 1,878,760 - -0.6 1,878,760 - +0.8 1,878,760 - -0.2 1,878,760 - +0.5 1,878,760 - +1.7 1,878,763 + -1.8 1,878,764 - +0.7 1,878,764 - -0.5 1,878,764 - -0.3 1,878,764 - -0.3 1,878,764 - -1.0 1,878,780 - -2.5 1,878,780 - +1.0 1,878,780 - +0.6 1,878,783 + -0.5 1,878,783 + +4.0 1,878,784 - +0.3 1,878,784 - -0.0 1,878,849 + +0.7 1,878,849 + -1.8 1,878,867 - +1.0 1,879,007 + -0.9 1,879,008 - -0.0 1,879,008 - +2.0 1,879,185 + +1.0 1,879,185 + +1.0 1,879,185 + -1.0 1,879,186 - +2.6 1,879,186 - -0.9 1,879,186 - -0.0 1,879,201 + +3.3 1,879,201 + -1.4 1,879,201 + -0.3 1,879,201 + -0.4 1,879,201 + -0.3 1,879,201 + +1.6 1,879,201 + +1.0 1,879,201 + +1.0 1,879,202 - -0.0 1,879,202 - -0.7 1,879,202 - +0.3 1,879,202 - -0.2 1,879,408 + +1.7 1,879,408 + +2.2 1,879,408 + +0.2 1,879,409 - +0.4 1,879,409 - -0.1 1,879,410 - +1.7 1,879,553 + +2.4 1,879,553 + -0.9 1,879,553 + +2.2 1,879,553 + -1.3 1,879,554 - -2.7 1,879,554 - +0.4 1,879,554 - +1.5 1,879,554 - +1.0 1,879,950 + RPSI07_RS16570 0.11 +1.0 1,879,951 - RPSI07_RS16570 0.11 +1.1 1,879,951 - RPSI07_RS16570 0.11 +1.7 1,879,951 - RPSI07_RS16570 0.11 -0.4 1,880,122 + RPSI07_RS16570 0.13 -0.2 1,880,122 + RPSI07_RS16570 0.13 +0.2 1,880,122 + RPSI07_RS16570 0.13 +0.4 1,880,122 + RPSI07_RS16570 0.13 +0.8 1,880,123 - RPSI07_RS16570 0.13 -1.0 1,880,123 - RPSI07_RS16570 0.13 -1.6 1,880,123 - RPSI07_RS16570 0.13 -0.0 1,880,129 + RPSI07_RS16570 0.13 -1.1 1,880,308 + RPSI07_RS16570 0.15 -0.2 1,880,686 + RPSI07_RS16570 0.19 +0.1 1,880,687 - RPSI07_RS16570 0.19 -0.2 1,880,729 + RPSI07_RS16570 0.19 +1.4 1,880,729 + RPSI07_RS16570 0.19 -0.2 1,880,730 - RPSI07_RS16570 0.19 +0.1 1,880,734 - RPSI07_RS16570 0.19 +1.5 1,880,926 + RPSI07_RS16570 0.21 -1.2 1,880,926 + RPSI07_RS16570 0.21 -1.0 1,881,502 + RPSI07_RS16570 0.28 -0.4 1,881,503 - RPSI07_RS16570 0.28 -0.3 1,881,503 - RPSI07_RS16570 0.28 -0.0 1,881,574 + RPSI07_RS16570 0.28 -0.6 1,881,612 - RPSI07_RS16570 0.29 -0.7 1,881,747 + RPSI07_RS16570 0.30 +0.6 1,881,747 + RPSI07_RS16570 0.30 -0.7 1,881,747 + RPSI07_RS16570 0.30 +0.8 1,881,747 + RPSI07_RS16570 0.30 -0.3 1,881,748 - RPSI07_RS16570 0.30 +1.7 1,881,748 - RPSI07_RS16570 0.30 +1.6 1,881,860 - RPSI07_RS16570 0.32 +0.7 1,881,860 - RPSI07_RS16570 0.32 -0.2 1,881,860 - RPSI07_RS16570 0.32 -0.4 1,881,907 + RPSI07_RS16570 0.32 -0.8 1,881,907 + RPSI07_RS16570 0.32 +0.1 1,881,907 + RPSI07_RS16570 0.32 -2.5 1,881,908 - RPSI07_RS16570 0.32 +0.8 1,882,051 + RPSI07_RS16570 0.34 +0.3 1,882,167 - RPSI07_RS16570 0.35 +0.2 1,882,188 + RPSI07_RS16570 0.35 -3.7 1,882,189 - RPSI07_RS16570 0.35 +1.0 1,882,264 + RPSI07_RS16570 0.36 +0.1 1,882,264 + RPSI07_RS16570 0.36 +0.6 1,882,265 - RPSI07_RS16570 0.36 -0.6 1,882,505 - RPSI07_RS16570 0.38 +0.7 1,882,505 - RPSI07_RS16570 0.38 +2.6 1,882,505 - RPSI07_RS16570 0.38 +0.0 1,882,510 + RPSI07_RS16570 0.39 +0.2 1,882,681 + RPSI07_RS16570 0.40 +0.2 1,882,681 + RPSI07_RS16570 0.40 -0.1 1,882,682 - RPSI07_RS16570 0.40 +1.3 1,882,682 - RPSI07_RS16570 0.40 -0.9 1,882,682 - RPSI07_RS16570 0.40 -0.5 1,882,682 - RPSI07_RS16570 0.40 -1.0 1,882,793 + RPSI07_RS16570 0.42 -0.9 1,882,793 + RPSI07_RS16570 0.42 -0.2 1,882,793 + RPSI07_RS16570 0.42 -0.3 1,882,880 - RPSI07_RS16570 0.43 -0.1 1,883,285 - RPSI07_RS16570 0.47 -1.5 1,883,529 - RPSI07_RS16570 0.50 -0.9 1,883,529 - RPSI07_RS16570 0.50 +0.1 1,883,529 - RPSI07_RS16570 0.50 -1.3 1,883,911 + RPSI07_RS16570 0.54 +1.0 1,883,912 - RPSI07_RS16570 0.54 +1.2 1,884,025 + RPSI07_RS16570 0.55 +0.7 1,884,025 + RPSI07_RS16570 0.55 +1.0 1,884,025 + RPSI07_RS16570 0.55 -2.1 1,884,025 + RPSI07_RS16570 0.55 -0.6 1,884,026 - RPSI07_RS16570 0.55 +0.4 1,884,026 - RPSI07_RS16570 0.55 +2.0 1,884,026 - RPSI07_RS16570 0.55 -1.0 1,884,029 - RPSI07_RS16570 0.55 -2.2 1,884,058 - RPSI07_RS16570 0.55 +0.8 1,884,681 - RPSI07_RS16570 0.62 +0.3 1,887,131 - RPSI07_RS16570 0.88 +0.4 1,887,328 + +0.3 1,887,328 + +0.3 1,887,328 + -2.0 1,887,329 - -2.7 1,887,329 - -0.0 1,887,329 - -1.5 1,887,329 - +2.8 1,887,364 + -0.3 1,887,365 - -0.5 1,887,365 - -0.8 1,887,403 + -0.0 1,887,403 + +0.3 1,887,403 + -0.6 1,887,403 + -1.1 1,887,403 + +0.6 1,887,491 - -0.6 1,887,491 - +1.0 1,887,491 - -0.4 1,887,618 + -0.9 1,887,618 + -0.6 1,887,618 + +2.3 1,887,618 + +0.9 1,887,619 - +0.7 1,887,761 + +0.1 1,887,762 - +0.5 1,887,762 - -1.0 1,887,762 - -1.6 1,887,821 - -1.0 1,888,172 + -2.8 1,888,172 + -2.2 1,888,172 + -0.1 1,888,172 + -3.6 1,888,172 + +2.6 1,888,173 - +1.3 1,888,173 - -0.3 1,888,209 - -2.0 1,888,300 + -2.4 1,888,301 - +1.2 1,888,404 + +0.2 1,888,405 - +0.5 1,888,405 - +0.7 1,888,443 - -0.1 1,888,443 - -0.8 1,888,443 - -1.3 1,888,486 + -0.4 1,888,486 + +0.4 1,888,487 - -1.5 1,888,514 + +2.6 1,888,514 + -1.3 1,888,514 + -0.1 1,888,515 - +1.0 1,888,515 - -2.0 1,888,597 + +1.0 1,888,597 + +0.5 1,888,597 + +1.2 1,888,598 - +0.1 1,888,665 + -1.0 1,888,665 + +1.7 1,888,665 + -2.3 1,888,666 - -1.5 1,888,666 - +0.1 1,888,819 + -0.8 1,888,984 + -1.5 1,888,985 - -1.4 1,889,089 + -0.1 1,889,089 + -0.6
Or see this region's nucleotide sequence