Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS16200 and RPSI07_RS16205 are separated by 51 nucleotides RPSI07_RS16205 and RPSI07_RS16210 are separated by 87 nucleotides RPSI07_RS16210 and RPSI07_RS16215 are separated by 203 nucleotides
RPSI07_RS16200: RPSI07_RS16200 - DUF1842 domain-containing protein, at 1,795,374 to 1,796,000
_RS16200
RPSI07_RS16205: RPSI07_RS16205 - DUF1842 domain-containing protein, at 1,796,052 to 1,796,633
_RS16205
RPSI07_RS16210: RPSI07_RS16210 - GDL motif peptide-associated radical SAM/SPASM maturase, at 1,796,721 to 1,798,196
_RS16210
RPSI07_RS16215: RPSI07_RS16215 - LysR family transcriptional regulator, at 1,798,400 to 1,799,320
_RS16215
Position (kb)
1796
1797
1798
1799 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1795.842 kb on + strand, within RPSI07_RS16200 at 1795.842 kb on + strand, within RPSI07_RS16200 at 1795.889 kb on + strand, within RPSI07_RS16200 at 1795.890 kb on - strand, within RPSI07_RS16200 at 1795.908 kb on + strand, within RPSI07_RS16200 at 1795.908 kb on + strand, within RPSI07_RS16200 at 1796.082 kb on + strand at 1796.082 kb on + strand at 1796.082 kb on + strand at 1796.082 kb on + strand at 1796.083 kb on - strand at 1796.083 kb on - strand at 1796.083 kb on - strand at 1796.083 kb on - strand at 1796.083 kb on - strand at 1796.083 kb on - strand at 1796.083 kb on - strand at 1796.142 kb on - strand, within RPSI07_RS16205 at 1796.376 kb on + strand, within RPSI07_RS16205 at 1796.383 kb on - strand, within RPSI07_RS16205 at 1796.454 kb on + strand, within RPSI07_RS16205 at 1796.475 kb on + strand, within RPSI07_RS16205 at 1796.475 kb on + strand, within RPSI07_RS16205 at 1796.475 kb on + strand, within RPSI07_RS16205 at 1796.475 kb on + strand, within RPSI07_RS16205 at 1796.475 kb on + strand, within RPSI07_RS16205 at 1796.476 kb on - strand, within RPSI07_RS16205 at 1796.721 kb on - strand at 1796.721 kb on - strand at 1796.751 kb on + strand at 1796.752 kb on - strand at 1796.778 kb on + strand at 1796.778 kb on + strand at 1796.778 kb on + strand at 1796.779 kb on - strand at 1796.779 kb on - strand at 1796.779 kb on - strand at 1796.779 kb on - strand at 1796.779 kb on - strand at 1796.779 kb on - strand at 1796.779 kb on - strand at 1796.779 kb on - strand at 1796.958 kb on + strand, within RPSI07_RS16210 at 1796.959 kb on - strand, within RPSI07_RS16210 at 1797.008 kb on + strand, within RPSI07_RS16210 at 1797.008 kb on + strand, within RPSI07_RS16210 at 1797.008 kb on + strand, within RPSI07_RS16210 at 1797.008 kb on + strand, within RPSI07_RS16210 at 1797.008 kb on + strand, within RPSI07_RS16210 at 1797.009 kb on - strand, within RPSI07_RS16210 at 1797.009 kb on - strand, within RPSI07_RS16210 at 1797.009 kb on - strand, within RPSI07_RS16210 at 1797.009 kb on - strand, within RPSI07_RS16210 at 1797.009 kb on - strand, within RPSI07_RS16210 at 1797.009 kb on - strand, within RPSI07_RS16210 at 1797.009 kb on - strand, within RPSI07_RS16210 at 1797.012 kb on + strand, within RPSI07_RS16210 at 1797.012 kb on + strand, within RPSI07_RS16210 at 1797.012 kb on + strand, within RPSI07_RS16210 at 1797.013 kb on - strand, within RPSI07_RS16210 at 1797.013 kb on - strand, within RPSI07_RS16210 at 1797.170 kb on + strand, within RPSI07_RS16210 at 1797.171 kb on - strand, within RPSI07_RS16210 at 1797.216 kb on - strand, within RPSI07_RS16210 at 1797.358 kb on - strand, within RPSI07_RS16210 at 1797.396 kb on + strand, within RPSI07_RS16210 at 1797.397 kb on - strand, within RPSI07_RS16210 at 1797.397 kb on - strand, within RPSI07_RS16210 at 1797.463 kb on - strand, within RPSI07_RS16210 at 1797.708 kb on + strand, within RPSI07_RS16210 at 1797.708 kb on + strand, within RPSI07_RS16210 at 1797.708 kb on + strand, within RPSI07_RS16210 at 1797.709 kb on - strand, within RPSI07_RS16210 at 1797.709 kb on - strand, within RPSI07_RS16210 at 1797.712 kb on - strand, within RPSI07_RS16210 at 1797.784 kb on - strand, within RPSI07_RS16210 at 1797.903 kb on - strand, within RPSI07_RS16210 at 1797.903 kb on - strand, within RPSI07_RS16210 at 1798.042 kb on - strand, within RPSI07_RS16210 at 1798.042 kb on - strand, within RPSI07_RS16210 at 1798.047 kb on + strand, within RPSI07_RS16210 at 1798.047 kb on + strand, within RPSI07_RS16210 at 1798.047 kb on + strand, within RPSI07_RS16210 at 1798.048 kb on - strand, within RPSI07_RS16210 at 1798.048 kb on - strand, within RPSI07_RS16210 at 1798.048 kb on - strand, within RPSI07_RS16210 at 1798.194 kb on + strand at 1798.195 kb on - strand at 1798.389 kb on + strand at 1798.389 kb on + strand at 1798.389 kb on + strand at 1798.389 kb on + strand at 1798.389 kb on + strand at 1798.389 kb on + strand at 1798.390 kb on - strand at 1798.390 kb on - strand at 1798.390 kb on - strand at 1798.390 kb on - strand at 1798.390 kb on - strand at 1798.401 kb on + strand at 1798.401 kb on + strand at 1798.402 kb on - strand at 1798.402 kb on - strand at 1798.500 kb on + strand, within RPSI07_RS16215 at 1798.635 kb on + strand, within RPSI07_RS16215 at 1798.647 kb on + strand, within RPSI07_RS16215 at 1798.704 kb on + strand, within RPSI07_RS16215 at 1798.744 kb on - strand, within RPSI07_RS16215 at 1798.744 kb on - strand, within RPSI07_RS16215
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 1,795,842 + RPSI07_RS16200 0.75 -1.4 1,795,842 + RPSI07_RS16200 0.75 +0.9 1,795,889 + RPSI07_RS16200 0.82 +2.2 1,795,890 - RPSI07_RS16200 0.82 +0.3 1,795,908 + RPSI07_RS16200 0.85 +0.3 1,795,908 + RPSI07_RS16200 0.85 +0.9 1,796,082 + -1.1 1,796,082 + -0.7 1,796,082 + -1.9 1,796,082 + +0.7 1,796,083 - -0.2 1,796,083 - -0.6 1,796,083 - +0.6 1,796,083 - -1.9 1,796,083 - +0.3 1,796,083 - -0.8 1,796,083 - -0.5 1,796,142 - RPSI07_RS16205 0.15 -0.1 1,796,376 + RPSI07_RS16205 0.56 +0.9 1,796,383 - RPSI07_RS16205 0.57 +0.4 1,796,454 + RPSI07_RS16205 0.69 -1.9 1,796,475 + RPSI07_RS16205 0.73 -0.1 1,796,475 + RPSI07_RS16205 0.73 -1.4 1,796,475 + RPSI07_RS16205 0.73 -1.6 1,796,475 + RPSI07_RS16205 0.73 -2.5 1,796,475 + RPSI07_RS16205 0.73 +1.8 1,796,476 - RPSI07_RS16205 0.73 -0.0 1,796,721 - -1.2 1,796,721 - -0.1 1,796,751 + -0.6 1,796,752 - +0.7 1,796,778 + -1.0 1,796,778 + +0.2 1,796,778 + +0.2 1,796,779 - -0.3 1,796,779 - -1.3 1,796,779 - +0.5 1,796,779 - -0.4 1,796,779 - -0.4 1,796,779 - -0.6 1,796,779 - +0.1 1,796,779 - -0.7 1,796,958 + RPSI07_RS16210 0.16 +0.6 1,796,959 - RPSI07_RS16210 0.16 +2.5 1,797,008 + RPSI07_RS16210 0.19 -0.7 1,797,008 + RPSI07_RS16210 0.19 +0.9 1,797,008 + RPSI07_RS16210 0.19 -1.1 1,797,008 + RPSI07_RS16210 0.19 +0.3 1,797,008 + RPSI07_RS16210 0.19 -0.1 1,797,009 - RPSI07_RS16210 0.20 -0.1 1,797,009 - RPSI07_RS16210 0.20 +1.3 1,797,009 - RPSI07_RS16210 0.20 -1.6 1,797,009 - RPSI07_RS16210 0.20 +1.4 1,797,009 - RPSI07_RS16210 0.20 +1.7 1,797,009 - RPSI07_RS16210 0.20 +0.4 1,797,009 - RPSI07_RS16210 0.20 -1.1 1,797,012 + RPSI07_RS16210 0.20 -0.3 1,797,012 + RPSI07_RS16210 0.20 +0.4 1,797,012 + RPSI07_RS16210 0.20 -0.7 1,797,013 - RPSI07_RS16210 0.20 +0.5 1,797,013 - RPSI07_RS16210 0.20 -0.2 1,797,170 + RPSI07_RS16210 0.30 -2.8 1,797,171 - RPSI07_RS16210 0.30 +0.9 1,797,216 - RPSI07_RS16210 0.34 -1.1 1,797,358 - RPSI07_RS16210 0.43 +1.6 1,797,396 + RPSI07_RS16210 0.46 +0.5 1,797,397 - RPSI07_RS16210 0.46 -0.9 1,797,397 - RPSI07_RS16210 0.46 -0.2 1,797,463 - RPSI07_RS16210 0.50 +0.4 1,797,708 + RPSI07_RS16210 0.67 -1.2 1,797,708 + RPSI07_RS16210 0.67 +0.0 1,797,708 + RPSI07_RS16210 0.67 +0.8 1,797,709 - RPSI07_RS16210 0.67 -1.4 1,797,709 - RPSI07_RS16210 0.67 -1.1 1,797,712 - RPSI07_RS16210 0.67 +0.0 1,797,784 - RPSI07_RS16210 0.72 -0.1 1,797,903 - RPSI07_RS16210 0.80 +0.9 1,797,903 - RPSI07_RS16210 0.80 -0.1 1,798,042 - RPSI07_RS16210 0.89 -2.1 1,798,042 - RPSI07_RS16210 0.89 -0.3 1,798,047 + RPSI07_RS16210 0.90 -0.8 1,798,047 + RPSI07_RS16210 0.90 +1.3 1,798,047 + RPSI07_RS16210 0.90 -0.4 1,798,048 - RPSI07_RS16210 0.90 -0.6 1,798,048 - RPSI07_RS16210 0.90 -0.8 1,798,048 - RPSI07_RS16210 0.90 -1.4 1,798,194 + -0.5 1,798,195 - +0.0 1,798,389 + +1.0 1,798,389 + -1.9 1,798,389 + -1.1 1,798,389 + -2.8 1,798,389 + +0.2 1,798,389 + -1.4 1,798,390 - +1.9 1,798,390 - +0.0 1,798,390 - +1.5 1,798,390 - -0.1 1,798,390 - -2.6 1,798,401 + +2.5 1,798,401 + +1.9 1,798,402 - +0.3 1,798,402 - +2.5 1,798,500 + RPSI07_RS16215 0.11 -1.1 1,798,635 + RPSI07_RS16215 0.26 +0.6 1,798,647 + RPSI07_RS16215 0.27 +1.5 1,798,704 + RPSI07_RS16215 0.33 +3.1 1,798,744 - RPSI07_RS16215 0.37 +1.2 1,798,744 - RPSI07_RS16215 0.37 -1.9
Or see this region's nucleotide sequence