Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS14800 and RPSI07_RS14805 are separated by 27 nucleotides RPSI07_RS14805 and RPSI07_RS14810 are separated by 192 nucleotides RPSI07_RS14810 and RPSI07_RS14815 are separated by 205 nucleotides
RPSI07_RS14800: RPSI07_RS14800 - YdcF family protein, at 1,493,334 to 1,494,131
_RS14800
RPSI07_RS14805: RPSI07_RS14805 - heme-binding protein, at 1,494,159 to 1,494,572
_RS14805
RPSI07_RS14810: RPSI07_RS14810 - hemin uptake protein HemP, at 1,494,765 to 1,495,043
_RS14810
RPSI07_RS14815: RPSI07_RS14815 - biopolymer transporter ExbD, at 1,495,249 to 1,495,662
_RS14815
Position (kb) 1494
1495 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1493.190 kb on + strand at 1493.236 kb on + strand at 1493.251 kb on - strand at 1493.251 kb on - strand at 1493.251 kb on - strand at 1493.340 kb on - strand at 1493.637 kb on + strand, within RPSI07_RS14800 at 1493.637 kb on + strand, within RPSI07_RS14800 at 1493.638 kb on - strand, within RPSI07_RS14800 at 1493.638 kb on - strand at 1493.638 kb on - strand, within RPSI07_RS14800 at 1493.900 kb on + strand, within RPSI07_RS14800 at 1493.901 kb on - strand, within RPSI07_RS14800 at 1493.901 kb on - strand, within RPSI07_RS14800 at 1493.901 kb on - strand, within RPSI07_RS14800 at 1493.901 kb on - strand, within RPSI07_RS14800 at 1493.901 kb on - strand, within RPSI07_RS14800 at 1493.901 kb on - strand, within RPSI07_RS14800 at 1493.904 kb on + strand, within RPSI07_RS14800 at 1493.904 kb on + strand, within RPSI07_RS14800 at 1493.904 kb on + strand, within RPSI07_RS14800 at 1493.905 kb on - strand, within RPSI07_RS14800 at 1493.905 kb on - strand, within RPSI07_RS14800 at 1493.919 kb on + strand, within RPSI07_RS14800 at 1493.919 kb on + strand, within RPSI07_RS14800 at 1494.028 kb on + strand, within RPSI07_RS14800 at 1494.029 kb on - strand, within RPSI07_RS14800 at 1494.381 kb on + strand, within RPSI07_RS14805 at 1494.381 kb on + strand, within RPSI07_RS14805 at 1494.382 kb on - strand, within RPSI07_RS14805 at 1494.766 kb on + strand at 1494.767 kb on - strand at 1494.767 kb on - strand at 1494.767 kb on - strand at 1494.810 kb on - strand, within RPSI07_RS14810 at 1494.810 kb on - strand, within RPSI07_RS14810 at 1494.810 kb on - strand, within RPSI07_RS14810 at 1494.810 kb on - strand, within RPSI07_RS14810 at 1494.811 kb on + strand, within RPSI07_RS14810 at 1494.812 kb on - strand, within RPSI07_RS14810 at 1494.990 kb on - strand, within RPSI07_RS14810 at 1495.038 kb on - strand at 1495.125 kb on + strand at 1495.184 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 1,493,190 + +1.0 1,493,236 + -1.0 1,493,251 - +0.3 1,493,251 - -0.8 1,493,251 - +0.7 1,493,340 - +0.7 1,493,637 + RPSI07_RS14800 0.38 -0.1 1,493,637 + RPSI07_RS14800 0.38 +0.7 1,493,638 - RPSI07_RS14800 0.38 -1.8 1,493,638 - -2.8 1,493,638 - RPSI07_RS14800 0.38 +3.2 1,493,900 + RPSI07_RS14800 0.71 -1.4 1,493,901 - RPSI07_RS14800 0.71 -0.6 1,493,901 - RPSI07_RS14800 0.71 -1.4 1,493,901 - RPSI07_RS14800 0.71 +3.3 1,493,901 - RPSI07_RS14800 0.71 -1.6 1,493,901 - RPSI07_RS14800 0.71 +0.2 1,493,901 - RPSI07_RS14800 0.71 -1.7 1,493,904 + RPSI07_RS14800 0.71 +3.0 1,493,904 + RPSI07_RS14800 0.71 +0.4 1,493,904 + RPSI07_RS14800 0.71 -0.3 1,493,905 - RPSI07_RS14800 0.72 -1.3 1,493,905 - RPSI07_RS14800 0.72 +4.7 1,493,919 + RPSI07_RS14800 0.73 -1.4 1,493,919 + RPSI07_RS14800 0.73 -1.3 1,494,028 + RPSI07_RS14800 0.87 -0.7 1,494,029 - RPSI07_RS14800 0.87 -2.0 1,494,381 + RPSI07_RS14805 0.54 +0.4 1,494,381 + RPSI07_RS14805 0.54 +0.3 1,494,382 - RPSI07_RS14805 0.54 -0.0 1,494,766 + -1.3 1,494,767 - -0.5 1,494,767 - -3.4 1,494,767 - +0.1 1,494,810 - RPSI07_RS14810 0.16 +0.6 1,494,810 - RPSI07_RS14810 0.16 +1.0 1,494,810 - RPSI07_RS14810 0.16 +0.9 1,494,810 - RPSI07_RS14810 0.16 +3.9 1,494,811 + RPSI07_RS14810 0.16 +0.5 1,494,812 - RPSI07_RS14810 0.17 +0.9 1,494,990 - RPSI07_RS14810 0.81 -0.1 1,495,038 - -2.0 1,495,125 + -1.2 1,495,184 + -1.2
Or see this region's nucleotide sequence