Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS11650 and RPSI07_RS11655 are separated by 256 nucleotides RPSI07_RS11655 and RPSI07_RS11660 are separated by 660 nucleotides
RPSI07_RS11650: RPSI07_RS11650 - UDP-glucuronosyltransferase, at 810,845 to 812,035
_RS11650
RPSI07_RS11655: RPSI07_RS11655 - glycosyl transferase family 1, at 812,292 to 813,626
_RS11655
RPSI07_RS11660: RPSI07_RS11660 - calcium-binding protein, at 814,287 to 823,115
_RS11660
Position (kb) 812
813
814 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 811.326 kb on + strand, within RPSI07_RS11650 at 811.404 kb on + strand, within RPSI07_RS11650 at 811.404 kb on + strand, within RPSI07_RS11650 at 811.404 kb on + strand, within RPSI07_RS11650 at 811.405 kb on - strand, within RPSI07_RS11650 at 811.405 kb on - strand, within RPSI07_RS11650 at 811.405 kb on - strand, within RPSI07_RS11650 at 811.512 kb on + strand, within RPSI07_RS11650 at 811.513 kb on - strand, within RPSI07_RS11650 at 811.788 kb on + strand, within RPSI07_RS11650 at 811.789 kb on - strand, within RPSI07_RS11650 at 811.789 kb on - strand, within RPSI07_RS11650 at 811.793 kb on + strand, within RPSI07_RS11650 at 811.815 kb on + strand, within RPSI07_RS11650 at 811.815 kb on + strand, within RPSI07_RS11650 at 811.816 kb on - strand, within RPSI07_RS11650 at 811.984 kb on + strand at 811.985 kb on - strand at 811.985 kb on - strand at 812.045 kb on - strand at 812.045 kb on - strand at 812.050 kb on + strand at 812.051 kb on - strand at 812.079 kb on - strand at 812.156 kb on + strand at 812.185 kb on + strand at 812.185 kb on + strand at 812.186 kb on - strand at 812.186 kb on - strand at 812.192 kb on + strand at 812.193 kb on - strand at 812.317 kb on - strand at 812.438 kb on + strand, within RPSI07_RS11655 at 812.439 kb on - strand, within RPSI07_RS11655 at 812.449 kb on + strand, within RPSI07_RS11655 at 812.450 kb on - strand, within RPSI07_RS11655 at 812.450 kb on - strand, within RPSI07_RS11655 at 812.524 kb on + strand, within RPSI07_RS11655 at 812.525 kb on - strand, within RPSI07_RS11655 at 812.525 kb on - strand, within RPSI07_RS11655 at 812.569 kb on - strand, within RPSI07_RS11655 at 812.569 kb on - strand, within RPSI07_RS11655 at 812.569 kb on - strand, within RPSI07_RS11655 at 812.569 kb on - strand, within RPSI07_RS11655 at 812.743 kb on - strand, within RPSI07_RS11655 at 812.743 kb on - strand, within RPSI07_RS11655 at 812.743 kb on - strand, within RPSI07_RS11655 at 812.745 kb on + strand, within RPSI07_RS11655 at 812.745 kb on + strand, within RPSI07_RS11655 at 812.745 kb on + strand, within RPSI07_RS11655 at 812.746 kb on - strand, within RPSI07_RS11655 at 812.746 kb on - strand, within RPSI07_RS11655 at 812.748 kb on + strand, within RPSI07_RS11655 at 812.749 kb on - strand, within RPSI07_RS11655 at 812.749 kb on - strand, within RPSI07_RS11655 at 812.913 kb on + strand, within RPSI07_RS11655 at 813.036 kb on + strand, within RPSI07_RS11655 at 813.036 kb on + strand, within RPSI07_RS11655 at 813.036 kb on + strand, within RPSI07_RS11655 at 813.037 kb on - strand, within RPSI07_RS11655 at 813.157 kb on - strand, within RPSI07_RS11655 at 813.255 kb on + strand, within RPSI07_RS11655 at 813.264 kb on + strand, within RPSI07_RS11655 at 813.300 kb on + strand, within RPSI07_RS11655 at 813.351 kb on - strand, within RPSI07_RS11655 at 813.377 kb on + strand, within RPSI07_RS11655 at 813.534 kb on + strand at 813.534 kb on + strand at 813.535 kb on - strand at 813.535 kb on - strand at 813.536 kb on + strand at 813.556 kb on - strand at 813.648 kb on - strand at 813.672 kb on - strand at 813.785 kb on + strand at 813.797 kb on + strand at 813.924 kb on + strand at 813.990 kb on + strand at 813.990 kb on + strand at 814.000 kb on - strand at 814.178 kb on - strand at 814.254 kb on - strand at 814.339 kb on - strand at 814.339 kb on - strand at 814.422 kb on + strand at 814.422 kb on + strand at 814.423 kb on - strand at 814.527 kb on + strand at 814.527 kb on + strand at 814.528 kb on - strand at 814.570 kb on + strand at 814.570 kb on + strand at 814.589 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 811,326 + RPSI07_RS11650 0.40 -2.3 811,404 + RPSI07_RS11650 0.47 +0.5 811,404 + RPSI07_RS11650 0.47 -1.3 811,404 + RPSI07_RS11650 0.47 +0.3 811,405 - RPSI07_RS11650 0.47 -1.0 811,405 - RPSI07_RS11650 0.47 -0.3 811,405 - RPSI07_RS11650 0.47 +1.0 811,512 + RPSI07_RS11650 0.56 -1.6 811,513 - RPSI07_RS11650 0.56 -1.5 811,788 + RPSI07_RS11650 0.79 +1.3 811,789 - RPSI07_RS11650 0.79 -0.0 811,789 - RPSI07_RS11650 0.79 +1.6 811,793 + RPSI07_RS11650 0.80 -0.1 811,815 + RPSI07_RS11650 0.81 -1.0 811,815 + RPSI07_RS11650 0.81 -0.4 811,816 - RPSI07_RS11650 0.82 -0.1 811,984 + +0.0 811,985 - +0.9 811,985 - +0.0 812,045 - +1.0 812,045 - +0.7 812,050 + +0.8 812,051 - +1.0 812,079 - -1.0 812,156 + -1.1 812,185 + -2.3 812,185 + -2.0 812,186 - -1.1 812,186 - +0.4 812,192 + +0.0 812,193 - +2.0 812,317 - -1.6 812,438 + RPSI07_RS11655 0.11 -0.2 812,439 - RPSI07_RS11655 0.11 -1.0 812,449 + RPSI07_RS11655 0.12 +0.6 812,450 - RPSI07_RS11655 0.12 -0.1 812,450 - RPSI07_RS11655 0.12 -0.8 812,524 + RPSI07_RS11655 0.17 +0.3 812,525 - RPSI07_RS11655 0.17 +1.0 812,525 - RPSI07_RS11655 0.17 +0.0 812,569 - RPSI07_RS11655 0.21 -0.3 812,569 - RPSI07_RS11655 0.21 -0.5 812,569 - RPSI07_RS11655 0.21 -1.9 812,569 - RPSI07_RS11655 0.21 +0.3 812,743 - RPSI07_RS11655 0.34 +1.0 812,743 - RPSI07_RS11655 0.34 +0.2 812,743 - RPSI07_RS11655 0.34 +0.0 812,745 + RPSI07_RS11655 0.34 -0.4 812,745 + RPSI07_RS11655 0.34 +1.5 812,745 + RPSI07_RS11655 0.34 +1.0 812,746 - RPSI07_RS11655 0.34 -2.5 812,746 - RPSI07_RS11655 0.34 -1.0 812,748 + RPSI07_RS11655 0.34 -1.3 812,749 - RPSI07_RS11655 0.34 +1.2 812,749 - RPSI07_RS11655 0.34 -0.4 812,913 + RPSI07_RS11655 0.47 +0.5 813,036 + RPSI07_RS11655 0.56 +1.0 813,036 + RPSI07_RS11655 0.56 +0.0 813,036 + RPSI07_RS11655 0.56 +0.0 813,037 - RPSI07_RS11655 0.56 +0.2 813,157 - RPSI07_RS11655 0.65 -1.1 813,255 + RPSI07_RS11655 0.72 +0.0 813,264 + RPSI07_RS11655 0.73 -1.0 813,300 + RPSI07_RS11655 0.76 +1.6 813,351 - RPSI07_RS11655 0.79 -1.0 813,377 + RPSI07_RS11655 0.81 +3.5 813,534 + +2.2 813,534 + +1.0 813,535 - +0.1 813,535 - +3.8 813,536 + +0.7 813,556 - +1.0 813,648 - -1.5 813,672 - +1.2 813,785 + +1.2 813,797 + +0.6 813,924 + -0.6 813,990 + -1.6 813,990 + +2.4 814,000 - +0.5 814,178 - -0.4 814,254 - +1.6 814,339 - +0.7 814,339 - +1.9 814,422 + +2.7 814,422 + -0.1 814,423 - +0.5 814,527 + +0.2 814,527 + +1.3 814,528 - +2.0 814,570 + -0.0 814,570 + -1.1 814,589 - -2.6
Or see this region's nucleotide sequence