Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS11135 and RPSI07_RS11140 are separated by 196 nucleotides RPSI07_RS11140 and RPSI07_RS11145 are separated by 37 nucleotides RPSI07_RS11145 and RPSI07_RS11150 overlap by 4 nucleotides
RPSI07_RS11135: RPSI07_RS11135 - signal recognition particle protein, at 696,624 to 698,063
_RS11135
RPSI07_RS11140: RPSI07_RS11140 - inner membrane protein YpjD, at 698,260 to 699,172
_RS11140
RPSI07_RS11145: RPSI07_RS11145 - hypothetical protein, at 699,210 to 699,467
_RS11145
RPSI07_RS11150: RPSI07_RS11150 - PAS domain-containing sensor histidine kinase, at 699,464 to 701,527
_RS11150
Position (kb) 698
699
700 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 698.270 kb on + strand at 698.270 kb on + strand at 698.270 kb on + strand at 698.270 kb on + strand at 698.270 kb on + strand at 698.270 kb on + strand at 698.270 kb on + strand at 698.270 kb on + strand at 698.271 kb on - strand at 698.271 kb on - strand at 698.271 kb on - strand at 698.271 kb on - strand at 698.271 kb on - strand at 698.271 kb on - strand at 698.272 kb on - strand at 698.315 kb on - strand at 698.315 kb on - strand at 698.540 kb on + strand, within RPSI07_RS11140 at 698.579 kb on + strand, within RPSI07_RS11140 at 698.580 kb on - strand, within RPSI07_RS11140 at 698.580 kb on - strand, within RPSI07_RS11140 at 698.809 kb on + strand, within RPSI07_RS11140 at 698.809 kb on + strand, within RPSI07_RS11140 at 698.810 kb on - strand, within RPSI07_RS11140 at 699.059 kb on + strand, within RPSI07_RS11140 at 699.125 kb on + strand at 699.125 kb on + strand at 699.126 kb on - strand at 699.168 kb on + strand at 699.168 kb on + strand at 699.168 kb on + strand at 699.323 kb on + strand, within RPSI07_RS11145 at 699.632 kb on + strand at 699.866 kb on + strand, within RPSI07_RS11150 at 699.867 kb on - strand, within RPSI07_RS11150 at 700.062 kb on - strand at 700.073 kb on + strand, within RPSI07_RS11150 at 700.073 kb on + strand, within RPSI07_RS11150 at 700.073 kb on + strand, within RPSI07_RS11150 at 700.073 kb on + strand, within RPSI07_RS11150 at 700.074 kb on - strand, within RPSI07_RS11150 at 700.074 kb on - strand, within RPSI07_RS11150 at 700.074 kb on - strand, within RPSI07_RS11150 at 700.097 kb on + strand, within RPSI07_RS11150 at 700.097 kb on + strand, within RPSI07_RS11150 at 700.098 kb on - strand, within RPSI07_RS11150 at 700.098 kb on - strand, within RPSI07_RS11150 at 700.098 kb on - strand, within RPSI07_RS11150 at 700.098 kb on - strand, within RPSI07_RS11150 at 700.100 kb on - strand, within RPSI07_RS11150
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 698,270 + -0.8 698,270 + -1.4 698,270 + +1.3 698,270 + +1.9 698,270 + -1.3 698,270 + +1.0 698,270 + +0.2 698,270 + -1.5 698,271 - -0.6 698,271 - -1.9 698,271 - +0.8 698,271 - +1.0 698,271 - +1.0 698,271 - +2.0 698,272 - -0.9 698,315 - +0.2 698,315 - +0.7 698,540 + RPSI07_RS11140 0.31 -2.0 698,579 + RPSI07_RS11140 0.35 +0.7 698,580 - RPSI07_RS11140 0.35 +0.3 698,580 - RPSI07_RS11140 0.35 +0.8 698,809 + RPSI07_RS11140 0.60 +2.0 698,809 + RPSI07_RS11140 0.60 -3.2 698,810 - RPSI07_RS11140 0.60 +0.7 699,059 + RPSI07_RS11140 0.88 +0.0 699,125 + -1.1 699,125 + -3.0 699,126 - -0.9 699,168 + -0.6 699,168 + -0.5 699,168 + +3.6 699,323 + RPSI07_RS11145 0.44 +1.8 699,632 + +0.2 699,866 + RPSI07_RS11150 0.19 +0.4 699,867 - RPSI07_RS11150 0.20 -1.5 700,062 - +0.1 700,073 + RPSI07_RS11150 0.30 -0.2 700,073 + RPSI07_RS11150 0.30 +0.4 700,073 + RPSI07_RS11150 0.30 -0.2 700,073 + RPSI07_RS11150 0.30 +0.3 700,074 - RPSI07_RS11150 0.30 +1.0 700,074 - RPSI07_RS11150 0.30 -1.3 700,074 - RPSI07_RS11150 0.30 -0.8 700,097 + RPSI07_RS11150 0.31 -0.5 700,097 + RPSI07_RS11150 0.31 +0.5 700,098 - RPSI07_RS11150 0.31 -0.2 700,098 - RPSI07_RS11150 0.31 -0.5 700,098 - RPSI07_RS11150 0.31 -0.9 700,098 - RPSI07_RS11150 0.31 -0.1 700,100 - RPSI07_RS11150 0.31 -0.5
Or see this region's nucleotide sequence