Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS06045 and RPSI07_RS06050 are separated by 161 nucleotides RPSI07_RS06050 and RPSI07_RS06055 are separated by 65 nucleotides RPSI07_RS06055 and RPSI07_RS06060 are separated by 873 nucleotides
RPSI07_RS06045: RPSI07_RS06045 - autotransporter domain-containing protein, at 1,641,082 to 1,644,129
_RS06045
RPSI07_RS06050: RPSI07_RS06050 - DNA-binding response regulator, at 1,644,291 to 1,644,983
_RS06050
RPSI07_RS06055: RPSI07_RS06055 - sensor histidine kinase, at 1,645,049 to 1,646,980
_RS06055
RPSI07_RS06060: RPSI07_RS06060 - type III effector protein (skwp2), at 1,647,854 to 1,655,209
_RS06060
Position (kb)
1645
1646
1647 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1644.130 kb on - strand at 1644.150 kb on + strand at 1644.151 kb on - strand at 1644.151 kb on - strand at 1644.185 kb on - strand at 1644.210 kb on + strand at 1644.210 kb on + strand at 1644.211 kb on - strand at 1644.211 kb on - strand at 1644.211 kb on - strand at 1644.212 kb on + strand at 1644.213 kb on - strand at 1644.213 kb on - strand at 1644.213 kb on - strand at 1644.213 kb on - strand at 1644.213 kb on - strand at 1644.252 kb on - strand at 1644.252 kb on - strand at 1644.277 kb on + strand at 1644.277 kb on + strand at 1644.625 kb on - strand, within RPSI07_RS06050 at 1644.625 kb on - strand, within RPSI07_RS06050 at 1644.912 kb on + strand, within RPSI07_RS06050 at 1644.912 kb on + strand, within RPSI07_RS06050 at 1644.913 kb on - strand, within RPSI07_RS06050 at 1644.913 kb on - strand, within RPSI07_RS06050 at 1644.951 kb on + strand at 1644.951 kb on + strand at 1644.951 kb on + strand at 1644.952 kb on - strand at 1644.952 kb on - strand at 1644.952 kb on - strand at 1644.952 kb on - strand at 1644.982 kb on - strand at 1644.982 kb on - strand at 1645.055 kb on + strand at 1645.055 kb on + strand at 1645.055 kb on + strand at 1645.085 kb on + strand at 1645.086 kb on + strand at 1645.086 kb on + strand at 1645.086 kb on + strand at 1645.086 kb on + strand at 1645.086 kb on + strand at 1645.086 kb on + strand at 1645.087 kb on - strand at 1645.087 kb on - strand at 1645.087 kb on - strand at 1645.087 kb on - strand at 1645.130 kb on + strand at 1645.130 kb on + strand at 1645.130 kb on + strand at 1645.131 kb on - strand at 1645.406 kb on + strand, within RPSI07_RS06055 at 1645.406 kb on + strand, within RPSI07_RS06055 at 1645.406 kb on + strand, within RPSI07_RS06055 at 1645.407 kb on - strand, within RPSI07_RS06055 at 1645.445 kb on + strand, within RPSI07_RS06055 at 1645.445 kb on + strand, within RPSI07_RS06055 at 1645.446 kb on - strand, within RPSI07_RS06055 at 1645.657 kb on - strand, within RPSI07_RS06055 at 1645.657 kb on - strand, within RPSI07_RS06055 at 1645.657 kb on - strand, within RPSI07_RS06055 at 1645.763 kb on + strand, within RPSI07_RS06055 at 1645.801 kb on - strand, within RPSI07_RS06055 at 1645.817 kb on + strand, within RPSI07_RS06055 at 1645.818 kb on - strand, within RPSI07_RS06055 at 1645.818 kb on - strand, within RPSI07_RS06055 at 1646.036 kb on + strand, within RPSI07_RS06055 at 1646.036 kb on + strand, within RPSI07_RS06055 at 1646.037 kb on - strand, within RPSI07_RS06055 at 1646.037 kb on - strand, within RPSI07_RS06055 at 1646.143 kb on + strand, within RPSI07_RS06055 at 1646.143 kb on + strand, within RPSI07_RS06055 at 1646.143 kb on + strand, within RPSI07_RS06055 at 1646.143 kb on + strand, within RPSI07_RS06055 at 1646.143 kb on + strand, within RPSI07_RS06055 at 1646.144 kb on - strand, within RPSI07_RS06055 at 1646.144 kb on - strand, within RPSI07_RS06055 at 1646.144 kb on - strand, within RPSI07_RS06055 at 1646.217 kb on - strand, within RPSI07_RS06055 at 1646.217 kb on - strand, within RPSI07_RS06055 at 1646.258 kb on + strand, within RPSI07_RS06055 at 1646.258 kb on + strand, within RPSI07_RS06055 at 1646.259 kb on - strand, within RPSI07_RS06055 at 1646.259 kb on - strand, within RPSI07_RS06055 at 1646.267 kb on + strand, within RPSI07_RS06055 at 1646.267 kb on + strand, within RPSI07_RS06055 at 1646.267 kb on + strand, within RPSI07_RS06055 at 1646.268 kb on - strand, within RPSI07_RS06055 at 1646.469 kb on + strand, within RPSI07_RS06055 at 1646.811 kb on + strand at 1646.835 kb on + strand at 1646.836 kb on - strand at 1647.046 kb on - strand at 1647.046 kb on - strand at 1647.141 kb on + strand at 1647.141 kb on + strand at 1647.141 kb on + strand at 1647.141 kb on + strand at 1647.141 kb on + strand at 1647.142 kb on - strand at 1647.142 kb on - strand at 1647.142 kb on - strand at 1647.147 kb on + strand at 1647.148 kb on - strand at 1647.232 kb on + strand at 1647.233 kb on - strand at 1647.335 kb on + strand at 1647.336 kb on - strand at 1647.336 kb on - strand at 1647.336 kb on - strand at 1647.363 kb on + strand at 1647.363 kb on + strand at 1647.363 kb on + strand at 1647.364 kb on - strand at 1647.364 kb on - strand at 1647.364 kb on - strand at 1647.781 kb on - strand at 1647.781 kb on - strand at 1647.786 kb on + strand at 1647.934 kb on + strand at 1647.935 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 1,644,130 - -0.9 1,644,150 + -0.7 1,644,151 - -1.8 1,644,151 - +0.9 1,644,185 - -1.1 1,644,210 + +0.2 1,644,210 + -1.7 1,644,211 - -1.8 1,644,211 - -1.4 1,644,211 - +2.0 1,644,212 + +0.9 1,644,213 - -1.2 1,644,213 - -0.5 1,644,213 - -0.8 1,644,213 - +1.5 1,644,213 - -0.7 1,644,252 - -0.5 1,644,252 - +1.2 1,644,277 + -0.3 1,644,277 + -0.1 1,644,625 - RPSI07_RS06050 0.48 +1.5 1,644,625 - RPSI07_RS06050 0.48 +0.4 1,644,912 + RPSI07_RS06050 0.90 +4.3 1,644,912 + RPSI07_RS06050 0.90 +1.0 1,644,913 - RPSI07_RS06050 0.90 +1.2 1,644,913 - RPSI07_RS06050 0.90 -0.0 1,644,951 + +0.9 1,644,951 + +0.4 1,644,951 + +2.0 1,644,952 - +1.9 1,644,952 - -1.6 1,644,952 - +1.4 1,644,952 - +0.4 1,644,982 - +0.2 1,644,982 - +0.9 1,645,055 + -2.3 1,645,055 + +1.8 1,645,055 + -0.5 1,645,085 + -0.7 1,645,086 + -0.1 1,645,086 + +0.9 1,645,086 + -1.4 1,645,086 + +0.9 1,645,086 + -1.6 1,645,086 + +0.1 1,645,087 - -0.1 1,645,087 - -0.7 1,645,087 - +0.5 1,645,087 - +0.2 1,645,130 + +0.9 1,645,130 + -0.1 1,645,130 + +2.1 1,645,131 - -0.2 1,645,406 + RPSI07_RS06055 0.18 +0.9 1,645,406 + RPSI07_RS06055 0.18 +2.3 1,645,406 + RPSI07_RS06055 0.18 -0.9 1,645,407 - RPSI07_RS06055 0.19 +0.8 1,645,445 + RPSI07_RS06055 0.20 +0.3 1,645,445 + RPSI07_RS06055 0.20 -0.5 1,645,446 - RPSI07_RS06055 0.21 +1.4 1,645,657 - RPSI07_RS06055 0.31 -0.7 1,645,657 - RPSI07_RS06055 0.31 +0.3 1,645,657 - RPSI07_RS06055 0.31 -1.1 1,645,763 + RPSI07_RS06055 0.37 +0.2 1,645,801 - RPSI07_RS06055 0.39 -0.3 1,645,817 + RPSI07_RS06055 0.40 +0.1 1,645,818 - RPSI07_RS06055 0.40 +0.7 1,645,818 - RPSI07_RS06055 0.40 +0.4 1,646,036 + RPSI07_RS06055 0.51 +0.1 1,646,036 + RPSI07_RS06055 0.51 +1.1 1,646,037 - RPSI07_RS06055 0.51 -2.2 1,646,037 - RPSI07_RS06055 0.51 +0.5 1,646,143 + RPSI07_RS06055 0.57 -1.4 1,646,143 + RPSI07_RS06055 0.57 +0.3 1,646,143 + RPSI07_RS06055 0.57 -0.5 1,646,143 + RPSI07_RS06055 0.57 -2.7 1,646,143 + RPSI07_RS06055 0.57 +0.9 1,646,144 - RPSI07_RS06055 0.57 +0.1 1,646,144 - RPSI07_RS06055 0.57 -0.3 1,646,144 - RPSI07_RS06055 0.57 -0.7 1,646,217 - RPSI07_RS06055 0.60 -0.2 1,646,217 - RPSI07_RS06055 0.60 -0.4 1,646,258 + RPSI07_RS06055 0.63 -0.1 1,646,258 + RPSI07_RS06055 0.63 +0.7 1,646,259 - RPSI07_RS06055 0.63 +0.5 1,646,259 - RPSI07_RS06055 0.63 -0.2 1,646,267 + RPSI07_RS06055 0.63 +2.9 1,646,267 + RPSI07_RS06055 0.63 +2.9 1,646,267 + RPSI07_RS06055 0.63 -3.0 1,646,268 - RPSI07_RS06055 0.63 -1.4 1,646,469 + RPSI07_RS06055 0.73 -1.1 1,646,811 + +0.9 1,646,835 + +1.7 1,646,836 - -0.8 1,647,046 - -2.4 1,647,046 - -0.1 1,647,141 + -0.4 1,647,141 + +1.5 1,647,141 + -0.1 1,647,141 + +0.9 1,647,141 + -0.8 1,647,142 - +0.2 1,647,142 - +0.2 1,647,142 - -1.4 1,647,147 + +0.1 1,647,148 - +0.0 1,647,232 + -1.6 1,647,233 - -0.3 1,647,335 + -1.7 1,647,336 - -0.0 1,647,336 - +0.1 1,647,336 - -0.1 1,647,363 + +1.2 1,647,363 + -0.9 1,647,363 + -0.2 1,647,364 - -1.7 1,647,364 - -2.2 1,647,364 - -1.3 1,647,781 - +2.0 1,647,781 - +0.7 1,647,786 + -0.2 1,647,934 + -1.1 1,647,935 - -0.4
Or see this region's nucleotide sequence