Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS05475

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS05470 and RPSI07_RS05475 are separated by 26 nucleotidesRPSI07_RS05475 and RPSI07_RS05480 are separated by 32 nucleotides RPSI07_RS05470: RPSI07_RS05470 - permease, at 1,489,467 to 1,490,819 _RS05470 RPSI07_RS05475: RPSI07_RS05475 - ketohydroxyglutarate aldolase, at 1,490,846 to 1,491,472 _RS05475 RPSI07_RS05480: RPSI07_RS05480 - 3,4-dihydroxy-2-butanone-4-phosphate synthase, at 1,491,505 to 1,492,230 _RS05480 Position (kb) 1490 1491 1492Strain fitness (log2 ratio) -1 0 1 2 3at 1490.016 kb on + strand, within RPSI07_RS05470at 1490.319 kb on + strand, within RPSI07_RS05470at 1490.319 kb on + strand, within RPSI07_RS05470at 1490.768 kb on + strandat 1490.768 kb on + strandat 1490.768 kb on + strandat 1490.768 kb on + strandat 1491.705 kb on + strand, within RPSI07_RS05480at 1491.822 kb on + strand, within RPSI07_RS05480at 1491.822 kb on + strand, within RPSI07_RS05480at 1491.823 kb on - strand, within RPSI07_RS05480at 1491.823 kb on - strand, within RPSI07_RS05480at 1491.823 kb on - strand, within RPSI07_RS05480

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
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1,490,016 + RPSI07_RS05470 0.41 +1.3
1,490,319 + RPSI07_RS05470 0.63 -0.3
1,490,319 + RPSI07_RS05470 0.63 +0.5
1,490,768 + +0.1
1,490,768 + -0.1
1,490,768 + +2.1
1,490,768 + -0.1
1,491,705 + RPSI07_RS05480 0.28 -0.3
1,491,822 + RPSI07_RS05480 0.44 -1.1
1,491,822 + RPSI07_RS05480 0.44 +3.7
1,491,823 - RPSI07_RS05480 0.44 +0.6
1,491,823 - RPSI07_RS05480 0.44 -0.2
1,491,823 - RPSI07_RS05480 0.44 +2.3

Or see this region's nucleotide sequence