Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS03870 and RPSI07_RS03875 are separated by 12 nucleotides RPSI07_RS03875 and RPSI07_RS03880 are separated by 372 nucleotides
RPSI07_RS03870: RPSI07_RS03870 - CusA/CzcA family heavy metal efflux RND transporter, at 1,050,963 to 1,054,118
_RS03870
RPSI07_RS03875: RPSI07_RS03875 - hypothetical protein, at 1,054,131 to 1,054,469
_RS03875
RPSI07_RS03880: RPSI07_RS03880 - ABC transporter substrate-binding protein, at 1,054,842 to 1,055,924
_RS03880
Position (kb)
1054
1055 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1053.435 kb on + strand, within RPSI07_RS03870 at 1053.435 kb on + strand, within RPSI07_RS03870 at 1053.435 kb on + strand, within RPSI07_RS03870 at 1053.435 kb on + strand, within RPSI07_RS03870 at 1053.435 kb on + strand, within RPSI07_RS03870 at 1053.436 kb on - strand, within RPSI07_RS03870 at 1053.522 kb on + strand, within RPSI07_RS03870 at 1053.523 kb on - strand, within RPSI07_RS03870 at 1053.552 kb on + strand, within RPSI07_RS03870 at 1053.553 kb on - strand, within RPSI07_RS03870 at 1053.616 kb on + strand, within RPSI07_RS03870 at 1053.617 kb on - strand, within RPSI07_RS03870 at 1053.624 kb on + strand, within RPSI07_RS03870 at 1053.624 kb on + strand, within RPSI07_RS03870 at 1053.625 kb on - strand at 1053.625 kb on - strand, within RPSI07_RS03870 at 1053.625 kb on - strand, within RPSI07_RS03870 at 1053.625 kb on - strand, within RPSI07_RS03870 at 1053.625 kb on - strand, within RPSI07_RS03870 at 1053.708 kb on + strand, within RPSI07_RS03870 at 1053.708 kb on + strand, within RPSI07_RS03870 at 1053.709 kb on - strand, within RPSI07_RS03870 at 1053.720 kb on + strand, within RPSI07_RS03870 at 1053.720 kb on + strand, within RPSI07_RS03870 at 1053.720 kb on + strand, within RPSI07_RS03870 at 1053.721 kb on - strand, within RPSI07_RS03870 at 1053.721 kb on - strand, within RPSI07_RS03870 at 1053.721 kb on - strand, within RPSI07_RS03870 at 1053.801 kb on + strand, within RPSI07_RS03870 at 1053.801 kb on + strand, within RPSI07_RS03870 at 1053.801 kb on + strand, within RPSI07_RS03870 at 1053.802 kb on - strand, within RPSI07_RS03870 at 1053.859 kb on + strand at 1053.860 kb on - strand at 1053.860 kb on - strand at 1053.896 kb on - strand at 1054.056 kb on + strand at 1054.057 kb on - strand at 1054.116 kb on + strand at 1054.117 kb on - strand at 1054.117 kb on - strand at 1054.127 kb on + strand at 1054.128 kb on - strand at 1054.128 kb on - strand at 1054.128 kb on - strand at 1054.173 kb on - strand, within RPSI07_RS03875 at 1054.408 kb on + strand, within RPSI07_RS03875 at 1054.568 kb on - strand at 1054.568 kb on - strand at 1054.580 kb on + strand at 1054.580 kb on + strand at 1054.581 kb on - strand at 1054.647 kb on + strand at 1054.649 kb on + strand at 1054.650 kb on - strand at 1054.650 kb on - strand at 1054.650 kb on - strand at 1054.674 kb on + strand at 1054.674 kb on + strand at 1054.675 kb on - strand at 1054.675 kb on - strand at 1054.715 kb on + strand at 1054.717 kb on + strand at 1054.743 kb on + strand at 1054.744 kb on - strand at 1054.744 kb on - strand at 1054.749 kb on + strand at 1054.750 kb on - strand at 1054.750 kb on - strand at 1054.787 kb on - strand at 1054.787 kb on - strand at 1055.041 kb on - strand, within RPSI07_RS03880 at 1055.070 kb on + strand, within RPSI07_RS03880 at 1055.070 kb on + strand, within RPSI07_RS03880 at 1055.070 kb on + strand, within RPSI07_RS03880 at 1055.071 kb on - strand, within RPSI07_RS03880 at 1055.071 kb on - strand, within RPSI07_RS03880 at 1055.091 kb on + strand, within RPSI07_RS03880 at 1055.121 kb on + strand, within RPSI07_RS03880 at 1055.122 kb on - strand, within RPSI07_RS03880 at 1055.289 kb on + strand, within RPSI07_RS03880 at 1055.289 kb on + strand, within RPSI07_RS03880 at 1055.289 kb on + strand, within RPSI07_RS03880 at 1055.289 kb on + strand, within RPSI07_RS03880
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Chloramphenicol_and_CrystalViolet remove 1,053,435 + RPSI07_RS03870 0.78 +0.1 1,053,435 + RPSI07_RS03870 0.78 -0.6 1,053,435 + RPSI07_RS03870 0.78 -0.5 1,053,435 + RPSI07_RS03870 0.78 -2.3 1,053,435 + RPSI07_RS03870 0.78 +1.4 1,053,436 - RPSI07_RS03870 0.78 -0.9 1,053,522 + RPSI07_RS03870 0.81 -0.0 1,053,523 - RPSI07_RS03870 0.81 -3.8 1,053,552 + RPSI07_RS03870 0.82 -2.0 1,053,553 - RPSI07_RS03870 0.82 -1.7 1,053,616 + RPSI07_RS03870 0.84 +0.5 1,053,617 - RPSI07_RS03870 0.84 -0.3 1,053,624 + RPSI07_RS03870 0.84 -1.0 1,053,624 + RPSI07_RS03870 0.84 -1.2 1,053,625 - -1.4 1,053,625 - RPSI07_RS03870 0.84 +1.3 1,053,625 - RPSI07_RS03870 0.84 -0.7 1,053,625 - RPSI07_RS03870 0.84 -0.4 1,053,625 - RPSI07_RS03870 0.84 +0.4 1,053,708 + RPSI07_RS03870 0.87 +1.3 1,053,708 + RPSI07_RS03870 0.87 +0.9 1,053,709 - RPSI07_RS03870 0.87 +0.9 1,053,720 + RPSI07_RS03870 0.87 -2.6 1,053,720 + RPSI07_RS03870 0.87 -1.1 1,053,720 + RPSI07_RS03870 0.87 +0.7 1,053,721 - RPSI07_RS03870 0.87 -0.6 1,053,721 - RPSI07_RS03870 0.87 -0.8 1,053,721 - RPSI07_RS03870 0.87 +1.7 1,053,801 + RPSI07_RS03870 0.90 -0.6 1,053,801 + RPSI07_RS03870 0.90 -2.4 1,053,801 + RPSI07_RS03870 0.90 +1.9 1,053,802 - RPSI07_RS03870 0.90 -0.7 1,053,859 + -0.4 1,053,860 - -0.5 1,053,860 - +2.2 1,053,896 - -0.5 1,054,056 + +0.9 1,054,057 - +0.3 1,054,116 + -0.3 1,054,117 - +2.5 1,054,117 - -1.7 1,054,127 + +0.4 1,054,128 - +0.9 1,054,128 - +0.8 1,054,128 - +1.9 1,054,173 - RPSI07_RS03875 0.12 +0.3 1,054,408 + RPSI07_RS03875 0.82 +0.3 1,054,568 - +0.1 1,054,568 - -0.2 1,054,580 + -0.1 1,054,580 + +0.4 1,054,581 - -0.0 1,054,647 + +1.0 1,054,649 + -2.7 1,054,650 - +0.3 1,054,650 - +1.5 1,054,650 - -0.5 1,054,674 + -2.4 1,054,674 + -0.2 1,054,675 - +0.9 1,054,675 - -1.4 1,054,715 + -0.1 1,054,717 + +0.7 1,054,743 + -0.1 1,054,744 - +2.2 1,054,744 - +1.1 1,054,749 + +1.1 1,054,750 - +0.9 1,054,750 - -0.7 1,054,787 - -0.1 1,054,787 - +0.3 1,055,041 - RPSI07_RS03880 0.18 -1.6 1,055,070 + RPSI07_RS03880 0.21 -0.6 1,055,070 + RPSI07_RS03880 0.21 -3.4 1,055,070 + RPSI07_RS03880 0.21 -0.3 1,055,071 - RPSI07_RS03880 0.21 +0.2 1,055,071 - RPSI07_RS03880 0.21 -0.1 1,055,091 + RPSI07_RS03880 0.23 -0.1 1,055,121 + RPSI07_RS03880 0.26 -1.0 1,055,122 - RPSI07_RS03880 0.26 -0.7 1,055,289 + RPSI07_RS03880 0.41 +0.1 1,055,289 + RPSI07_RS03880 0.41 +1.6 1,055,289 + RPSI07_RS03880 0.41 +0.1 1,055,289 + RPSI07_RS03880 0.41 -4.2
Or see this region's nucleotide sequence