Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS02725 and RPSI07_RS02730 are separated by 25 nucleotides RPSI07_RS02730 and RPSI07_RS24295 are separated by 35 nucleotides RPSI07_RS24295 and RPSI07_RS02735 overlap by 19 nucleotides
RPSI07_RS02725: RPSI07_RS02725 - porin, at 764,841 to 766,217
_RS02725
RPSI07_RS02730: RPSI07_RS02730 - FMN-binding protein, at 766,243 to 766,803
_RS02730
RPSI07_RS24295: RPSI07_RS24295 - hypothetical protein, at 766,839 to 767,255
_RS24295
RPSI07_RS02735: RPSI07_RS02735 - lipase, at 767,237 to 768,370
_RS02735
Position (kb) 766
767
768 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 765.855 kb on + strand, within RPSI07_RS02725 at 765.855 kb on + strand, within RPSI07_RS02725 at 765.855 kb on + strand, within RPSI07_RS02725 at 765.856 kb on - strand, within RPSI07_RS02725 at 765.856 kb on - strand, within RPSI07_RS02725 at 765.856 kb on - strand, within RPSI07_RS02725 at 765.856 kb on - strand, within RPSI07_RS02725 at 765.856 kb on - strand, within RPSI07_RS02725 at 765.856 kb on - strand, within RPSI07_RS02725 at 765.861 kb on + strand, within RPSI07_RS02725 at 765.862 kb on - strand, within RPSI07_RS02725 at 765.862 kb on - strand, within RPSI07_RS02725 at 765.879 kb on - strand, within RPSI07_RS02725 at 765.936 kb on + strand, within RPSI07_RS02725 at 765.936 kb on + strand, within RPSI07_RS02725 at 765.936 kb on + strand, within RPSI07_RS02725 at 765.936 kb on + strand, within RPSI07_RS02725 at 765.936 kb on + strand, within RPSI07_RS02725 at 765.937 kb on - strand, within RPSI07_RS02725 at 765.937 kb on - strand, within RPSI07_RS02725 at 765.945 kb on + strand, within RPSI07_RS02725 at 765.945 kb on + strand, within RPSI07_RS02725 at 765.945 kb on + strand, within RPSI07_RS02725 at 765.946 kb on - strand, within RPSI07_RS02725 at 765.946 kb on - strand, within RPSI07_RS02725 at 765.960 kb on + strand, within RPSI07_RS02725 at 765.960 kb on + strand, within RPSI07_RS02725 at 765.961 kb on - strand, within RPSI07_RS02725 at 766.009 kb on - strand, within RPSI07_RS02725 at 766.017 kb on + strand, within RPSI07_RS02725 at 766.017 kb on + strand, within RPSI07_RS02725 at 766.018 kb on - strand, within RPSI07_RS02725 at 766.035 kb on + strand, within RPSI07_RS02725 at 766.035 kb on + strand, within RPSI07_RS02725 at 766.036 kb on - strand, within RPSI07_RS02725 at 766.084 kb on + strand at 766.084 kb on + strand at 766.086 kb on + strand at 766.086 kb on + strand at 766.086 kb on + strand at 766.086 kb on + strand at 766.086 kb on + strand at 766.087 kb on - strand at 766.087 kb on - strand at 766.087 kb on - strand at 766.087 kb on - strand at 766.087 kb on - strand at 766.087 kb on - strand at 766.088 kb on - strand at 766.091 kb on + strand at 766.098 kb on + strand at 766.098 kb on + strand at 766.098 kb on + strand at 766.098 kb on + strand at 766.098 kb on + strand at 766.098 kb on + strand at 766.098 kb on + strand at 766.099 kb on - strand at 766.099 kb on - strand at 766.119 kb on + strand at 766.119 kb on + strand at 766.119 kb on + strand at 766.120 kb on - strand at 766.120 kb on - strand at 766.120 kb on - strand at 766.122 kb on - strand at 766.216 kb on - strand at 766.216 kb on - strand at 766.216 kb on - strand at 766.321 kb on + strand, within RPSI07_RS02730 at 766.322 kb on - strand, within RPSI07_RS02730 at 766.333 kb on + strand, within RPSI07_RS02730 at 766.333 kb on + strand, within RPSI07_RS02730 at 766.333 kb on + strand, within RPSI07_RS02730 at 766.334 kb on - strand, within RPSI07_RS02730 at 766.334 kb on - strand, within RPSI07_RS02730 at 766.334 kb on - strand, within RPSI07_RS02730 at 766.334 kb on - strand, within RPSI07_RS02730 at 766.510 kb on + strand, within RPSI07_RS02730 at 766.510 kb on + strand, within RPSI07_RS02730 at 766.555 kb on + strand, within RPSI07_RS02730 at 766.556 kb on - strand, within RPSI07_RS02730 at 766.556 kb on - strand, within RPSI07_RS02730 at 766.556 kb on - strand, within RPSI07_RS02730 at 766.609 kb on + strand, within RPSI07_RS02730 at 766.609 kb on + strand, within RPSI07_RS02730 at 766.610 kb on - strand, within RPSI07_RS02730 at 766.936 kb on + strand, within RPSI07_RS24295 at 766.936 kb on + strand, within RPSI07_RS24295 at 766.936 kb on + strand, within RPSI07_RS24295 at 766.936 kb on + strand, within RPSI07_RS24295 at 766.936 kb on + strand, within RPSI07_RS24295 at 766.936 kb on + strand, within RPSI07_RS24295 at 766.936 kb on + strand, within RPSI07_RS24295 at 766.936 kb on + strand, within RPSI07_RS24295 at 766.936 kb on + strand, within RPSI07_RS24295 at 766.936 kb on + strand, within RPSI07_RS24295 at 766.936 kb on + strand, within RPSI07_RS24295 at 766.936 kb on + strand, within RPSI07_RS24295 at 766.937 kb on - strand, within RPSI07_RS24295 at 766.937 kb on - strand, within RPSI07_RS24295 at 766.937 kb on - strand, within RPSI07_RS24295 at 766.937 kb on - strand, within RPSI07_RS24295 at 766.937 kb on - strand, within RPSI07_RS24295 at 767.013 kb on - strand, within RPSI07_RS24295 at 767.177 kb on + strand, within RPSI07_RS24295 at 767.177 kb on + strand, within RPSI07_RS24295 at 767.177 kb on + strand, within RPSI07_RS24295 at 767.178 kb on - strand, within RPSI07_RS24295 at 767.409 kb on - strand, within RPSI07_RS02735 at 767.605 kb on + strand, within RPSI07_RS02735 at 767.606 kb on - strand, within RPSI07_RS02735 at 767.606 kb on - strand, within RPSI07_RS02735 at 767.606 kb on - strand, within RPSI07_RS02735 at 767.606 kb on - strand, within RPSI07_RS02735 at 767.827 kb on + strand, within RPSI07_RS02735 at 767.846 kb on - strand, within RPSI07_RS02735 at 767.924 kb on + strand, within RPSI07_RS02735 at 767.924 kb on + strand, within RPSI07_RS02735 at 768.083 kb on + strand, within RPSI07_RS02735 at 768.083 kb on + strand, within RPSI07_RS02735 at 768.083 kb on + strand, within RPSI07_RS02735 at 768.083 kb on + strand, within RPSI07_RS02735 at 768.083 kb on + strand, within RPSI07_RS02735 at 768.083 kb on + strand, within RPSI07_RS02735 at 768.083 kb on + strand, within RPSI07_RS02735 at 768.083 kb on + strand, within RPSI07_RS02735 at 768.083 kb on + strand, within RPSI07_RS02735 at 768.083 kb on + strand, within RPSI07_RS02735 at 768.083 kb on + strand, within RPSI07_RS02735 at 768.083 kb on + strand, within RPSI07_RS02735 at 768.084 kb on - strand, within RPSI07_RS02735 at 768.084 kb on - strand, within RPSI07_RS02735 at 768.084 kb on - strand, within RPSI07_RS02735 at 768.122 kb on + strand, within RPSI07_RS02735 at 768.122 kb on + strand, within RPSI07_RS02735 at 768.122 kb on + strand, within RPSI07_RS02735 at 768.122 kb on + strand, within RPSI07_RS02735 at 768.122 kb on + strand, within RPSI07_RS02735 at 768.122 kb on + strand, within RPSI07_RS02735 at 768.122 kb on + strand, within RPSI07_RS02735 at 768.122 kb on + strand, within RPSI07_RS02735 at 768.123 kb on - strand, within RPSI07_RS02735 at 768.123 kb on - strand, within RPSI07_RS02735 at 768.123 kb on - strand, within RPSI07_RS02735 at 768.123 kb on - strand, within RPSI07_RS02735
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 765,855 + RPSI07_RS02725 0.74 -1.7 765,855 + RPSI07_RS02725 0.74 -0.8 765,855 + RPSI07_RS02725 0.74 +0.2 765,856 - RPSI07_RS02725 0.74 +0.8 765,856 - RPSI07_RS02725 0.74 -0.8 765,856 - RPSI07_RS02725 0.74 -0.8 765,856 - RPSI07_RS02725 0.74 -0.1 765,856 - RPSI07_RS02725 0.74 +5.3 765,856 - RPSI07_RS02725 0.74 +0.1 765,861 + RPSI07_RS02725 0.74 +1.2 765,862 - RPSI07_RS02725 0.74 +0.3 765,862 - RPSI07_RS02725 0.74 -1.2 765,879 - RPSI07_RS02725 0.75 -1.2 765,936 + RPSI07_RS02725 0.80 -0.5 765,936 + RPSI07_RS02725 0.80 +0.9 765,936 + RPSI07_RS02725 0.80 -0.1 765,936 + RPSI07_RS02725 0.80 -2.6 765,936 + RPSI07_RS02725 0.80 -1.2 765,937 - RPSI07_RS02725 0.80 -1.6 765,937 - RPSI07_RS02725 0.80 +0.1 765,945 + RPSI07_RS02725 0.80 -0.7 765,945 + RPSI07_RS02725 0.80 +1.8 765,945 + RPSI07_RS02725 0.80 -1.1 765,946 - RPSI07_RS02725 0.80 +1.6 765,946 - RPSI07_RS02725 0.80 +0.4 765,960 + RPSI07_RS02725 0.81 -0.8 765,960 + RPSI07_RS02725 0.81 +2.0 765,961 - RPSI07_RS02725 0.81 -0.5 766,009 - RPSI07_RS02725 0.85 -0.2 766,017 + RPSI07_RS02725 0.85 -1.0 766,017 + RPSI07_RS02725 0.85 +1.8 766,018 - RPSI07_RS02725 0.85 +0.4 766,035 + RPSI07_RS02725 0.87 +0.9 766,035 + RPSI07_RS02725 0.87 +0.5 766,036 - RPSI07_RS02725 0.87 +0.6 766,084 + -0.2 766,084 + -1.8 766,086 + -1.4 766,086 + +0.2 766,086 + -1.0 766,086 + -3.3 766,086 + -0.3 766,087 - -0.3 766,087 - +1.0 766,087 - -0.1 766,087 - +1.1 766,087 - -0.9 766,087 - +1.8 766,088 - +1.1 766,091 + -1.6 766,098 + -0.7 766,098 + -1.2 766,098 + +0.3 766,098 + -0.1 766,098 + -0.3 766,098 + -0.4 766,098 + +0.4 766,099 - -1.2 766,099 - +1.2 766,119 + -1.0 766,119 + +1.4 766,119 + -1.5 766,120 - -0.8 766,120 - -1.7 766,120 - -2.5 766,122 - -1.0 766,216 - +1.8 766,216 - -0.8 766,216 - -0.2 766,321 + RPSI07_RS02730 0.14 -0.4 766,322 - RPSI07_RS02730 0.14 +0.3 766,333 + RPSI07_RS02730 0.16 +0.5 766,333 + RPSI07_RS02730 0.16 +0.3 766,333 + RPSI07_RS02730 0.16 -0.3 766,334 - RPSI07_RS02730 0.16 +1.2 766,334 - RPSI07_RS02730 0.16 -1.4 766,334 - RPSI07_RS02730 0.16 +1.1 766,334 - RPSI07_RS02730 0.16 +2.1 766,510 + RPSI07_RS02730 0.48 +1.5 766,510 + RPSI07_RS02730 0.48 -1.1 766,555 + RPSI07_RS02730 0.56 -0.2 766,556 - RPSI07_RS02730 0.56 +0.7 766,556 - RPSI07_RS02730 0.56 +0.7 766,556 - RPSI07_RS02730 0.56 +0.3 766,609 + RPSI07_RS02730 0.65 +0.2 766,609 + RPSI07_RS02730 0.65 +1.2 766,610 - RPSI07_RS02730 0.65 +1.4 766,936 + RPSI07_RS24295 0.23 -1.1 766,936 + RPSI07_RS24295 0.23 -1.9 766,936 + RPSI07_RS24295 0.23 -0.1 766,936 + RPSI07_RS24295 0.23 -0.2 766,936 + RPSI07_RS24295 0.23 -1.7 766,936 + RPSI07_RS24295 0.23 -1.6 766,936 + RPSI07_RS24295 0.23 -1.2 766,936 + RPSI07_RS24295 0.23 -0.9 766,936 + RPSI07_RS24295 0.23 -2.0 766,936 + RPSI07_RS24295 0.23 -0.2 766,936 + RPSI07_RS24295 0.23 -0.7 766,936 + RPSI07_RS24295 0.23 -0.8 766,937 - RPSI07_RS24295 0.24 -0.3 766,937 - RPSI07_RS24295 0.24 -2.3 766,937 - RPSI07_RS24295 0.24 -3.9 766,937 - RPSI07_RS24295 0.24 +0.8 766,937 - RPSI07_RS24295 0.24 +0.2 767,013 - RPSI07_RS24295 0.42 -0.2 767,177 + RPSI07_RS24295 0.81 +0.5 767,177 + RPSI07_RS24295 0.81 +0.7 767,177 + RPSI07_RS24295 0.81 -0.6 767,178 - RPSI07_RS24295 0.81 -0.2 767,409 - RPSI07_RS02735 0.15 -1.4 767,605 + RPSI07_RS02735 0.32 +1.3 767,606 - RPSI07_RS02735 0.33 -1.6 767,606 - RPSI07_RS02735 0.33 -0.1 767,606 - RPSI07_RS02735 0.33 +3.1 767,606 - RPSI07_RS02735 0.33 +0.2 767,827 + RPSI07_RS02735 0.52 -0.8 767,846 - RPSI07_RS02735 0.54 +0.0 767,924 + RPSI07_RS02735 0.61 +0.3 767,924 + RPSI07_RS02735 0.61 -0.1 768,083 + RPSI07_RS02735 0.75 -1.3 768,083 + RPSI07_RS02735 0.75 +1.6 768,083 + RPSI07_RS02735 0.75 -0.2 768,083 + RPSI07_RS02735 0.75 +0.7 768,083 + RPSI07_RS02735 0.75 +0.1 768,083 + RPSI07_RS02735 0.75 -0.2 768,083 + RPSI07_RS02735 0.75 -0.2 768,083 + RPSI07_RS02735 0.75 -1.1 768,083 + RPSI07_RS02735 0.75 -1.2 768,083 + RPSI07_RS02735 0.75 +0.0 768,083 + RPSI07_RS02735 0.75 -1.2 768,083 + RPSI07_RS02735 0.75 +0.8 768,084 - RPSI07_RS02735 0.75 -2.1 768,084 - RPSI07_RS02735 0.75 +0.3 768,084 - RPSI07_RS02735 0.75 +0.0 768,122 + RPSI07_RS02735 0.78 -0.8 768,122 + RPSI07_RS02735 0.78 -1.1 768,122 + RPSI07_RS02735 0.78 -0.7 768,122 + RPSI07_RS02735 0.78 -0.2 768,122 + RPSI07_RS02735 0.78 +0.8 768,122 + RPSI07_RS02735 0.78 -0.6 768,122 + RPSI07_RS02735 0.78 +0.4 768,122 + RPSI07_RS02735 0.78 -1.8 768,123 - RPSI07_RS02735 0.78 +0.5 768,123 - RPSI07_RS02735 0.78 +0.8 768,123 - RPSI07_RS02735 0.78 -0.0 768,123 - RPSI07_RS02735 0.78 +0.9
Or see this region's nucleotide sequence