Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21280 and RPSI07_RS21285 are separated by 50 nucleotides RPSI07_RS21285 and RPSI07_RS21290 are separated by 118 nucleotides RPSI07_RS21290 and RPSI07_RS21295 are separated by 441 nucleotides RPSI07_RS21295 and RPSI07_RS21300 are separated by 21 nucleotides RPSI07_RS21300 and RPSI07_RS21305 are separated by 68 nucleotides
RPSI07_RS21280: RPSI07_RS21280 - MFS transporter, at 2,936,973 to 2,938,676
_RS21280
RPSI07_RS21285: RPSI07_RS21285 - tRNA-Met, at 2,938,727 to 2,938,803
_RS21285
RPSI07_RS21290: RPSI07_RS21290 - hypothetical protein, at 2,938,922 to 2,939,134
_RS21290
RPSI07_RS21295: RPSI07_RS21295 - FAD-binding monooxygenase, at 2,939,576 to 2,940,772
_RS21295
RPSI07_RS21300: RPSI07_RS21300 - hypothetical protein, at 2,940,794 to 2,941,354
_RS21300
RPSI07_RS21305: RPSI07_RS21305 - DNA-binding response regulator, at 2,941,423 to 2,942,163
_RS21305
Position (kb)
2939
2940
2941 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2938.662 kb on + strand at 2938.662 kb on + strand at 2938.663 kb on - strand at 2938.663 kb on - strand at 2938.663 kb on - strand at 2938.663 kb on - strand at 2938.986 kb on + strand, within RPSI07_RS21290 at 2939.134 kb on + strand at 2939.343 kb on + strand at 2939.344 kb on - strand at 2939.494 kb on + strand at 2939.495 kb on - strand at 2939.495 kb on - strand at 2939.495 kb on - strand at 2939.545 kb on + strand at 2939.545 kb on + strand at 2939.970 kb on - strand, within RPSI07_RS21295 at 2940.092 kb on + strand, within RPSI07_RS21295 at 2940.416 kb on + strand, within RPSI07_RS21295 at 2940.417 kb on - strand, within RPSI07_RS21295 at 2940.581 kb on + strand, within RPSI07_RS21295 at 2940.582 kb on - strand, within RPSI07_RS21295 at 2941.274 kb on + strand, within RPSI07_RS21300 at 2941.275 kb on - strand, within RPSI07_RS21300 at 2941.352 kb on + strand at 2941.352 kb on + strand at 2941.353 kb on - strand at 2941.353 kb on - strand at 2941.425 kb on - strand at 2941.500 kb on - strand, within RPSI07_RS21305 at 2941.511 kb on + strand, within RPSI07_RS21305 at 2941.511 kb on + strand, within RPSI07_RS21305 at 2941.512 kb on - strand, within RPSI07_RS21305 at 2941.512 kb on - strand, within RPSI07_RS21305 at 2941.512 kb on - strand, within RPSI07_RS21305 at 2941.673 kb on - strand, within RPSI07_RS21305 at 2941.673 kb on - strand, within RPSI07_RS21305 at 2941.673 kb on - strand, within RPSI07_RS21305
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 2,938,662 + -0.9 2,938,662 + +0.1 2,938,663 - +0.3 2,938,663 - +0.1 2,938,663 - +0.2 2,938,663 - +0.4 2,938,986 + RPSI07_RS21290 0.30 -0.8 2,939,134 + +1.1 2,939,343 + +2.0 2,939,344 - -0.8 2,939,494 + -0.8 2,939,495 - +2.0 2,939,495 - -0.4 2,939,495 - -0.5 2,939,545 + +0.0 2,939,545 + -1.7 2,939,970 - RPSI07_RS21295 0.33 -2.3 2,940,092 + RPSI07_RS21295 0.43 -0.2 2,940,416 + RPSI07_RS21295 0.70 -0.3 2,940,417 - RPSI07_RS21295 0.70 -0.5 2,940,581 + RPSI07_RS21295 0.84 +0.6 2,940,582 - RPSI07_RS21295 0.84 +0.8 2,941,274 + RPSI07_RS21300 0.86 -0.4 2,941,275 - RPSI07_RS21300 0.86 -1.1 2,941,352 + +0.2 2,941,352 + +0.0 2,941,353 - -0.2 2,941,353 - -1.2 2,941,425 - -1.7 2,941,500 - RPSI07_RS21305 0.10 +0.3 2,941,511 + RPSI07_RS21305 0.12 -0.3 2,941,511 + RPSI07_RS21305 0.12 -0.8 2,941,512 - RPSI07_RS21305 0.12 -2.3 2,941,512 - RPSI07_RS21305 0.12 -1.4 2,941,512 - RPSI07_RS21305 0.12 -2.9 2,941,673 - RPSI07_RS21305 0.34 -1.1 2,941,673 - RPSI07_RS21305 0.34 -0.8 2,941,673 - RPSI07_RS21305 0.34 -0.2
Or see this region's nucleotide sequence