Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21280 and RPSI07_RS21285 are separated by 50 nucleotides RPSI07_RS21285 and RPSI07_RS21290 are separated by 118 nucleotides RPSI07_RS21290 and RPSI07_RS21295 are separated by 441 nucleotides
RPSI07_RS21280: RPSI07_RS21280 - MFS transporter, at 2,936,973 to 2,938,676
_RS21280
RPSI07_RS21285: RPSI07_RS21285 - tRNA-Met, at 2,938,727 to 2,938,803
_RS21285
RPSI07_RS21290: RPSI07_RS21290 - hypothetical protein, at 2,938,922 to 2,939,134
_RS21290
RPSI07_RS21295: RPSI07_RS21295 - FAD-binding monooxygenase, at 2,939,576 to 2,940,772
_RS21295
Position (kb)
2938
2939 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2937.781 kb on - strand, within RPSI07_RS21280 at 2937.781 kb on - strand, within RPSI07_RS21280 at 2937.781 kb on - strand, within RPSI07_RS21280 at 2937.795 kb on + strand, within RPSI07_RS21280 at 2937.795 kb on + strand, within RPSI07_RS21280 at 2937.878 kb on + strand, within RPSI07_RS21280 at 2937.969 kb on + strand, within RPSI07_RS21280 at 2937.970 kb on - strand, within RPSI07_RS21280 at 2937.970 kb on - strand, within RPSI07_RS21280 at 2938.136 kb on + strand, within RPSI07_RS21280 at 2938.137 kb on - strand, within RPSI07_RS21280 at 2938.161 kb on + strand, within RPSI07_RS21280 at 2938.161 kb on + strand, within RPSI07_RS21280 at 2938.161 kb on + strand, within RPSI07_RS21280 at 2938.161 kb on + strand, within RPSI07_RS21280 at 2938.162 kb on - strand, within RPSI07_RS21280 at 2938.162 kb on - strand, within RPSI07_RS21280 at 2938.162 kb on - strand, within RPSI07_RS21280 at 2938.162 kb on - strand, within RPSI07_RS21280 at 2938.162 kb on - strand, within RPSI07_RS21280 at 2938.162 kb on - strand, within RPSI07_RS21280 at 2938.335 kb on + strand, within RPSI07_RS21280 at 2938.335 kb on + strand, within RPSI07_RS21280 at 2938.407 kb on + strand, within RPSI07_RS21280 at 2938.408 kb on - strand, within RPSI07_RS21280 at 2938.408 kb on - strand, within RPSI07_RS21280 at 2938.408 kb on - strand, within RPSI07_RS21280 at 2938.423 kb on - strand, within RPSI07_RS21280 at 2938.423 kb on - strand, within RPSI07_RS21280 at 2938.423 kb on - strand, within RPSI07_RS21280 at 2938.423 kb on - strand, within RPSI07_RS21280 at 2938.473 kb on + strand, within RPSI07_RS21280 at 2938.475 kb on + strand, within RPSI07_RS21280 at 2938.475 kb on + strand, within RPSI07_RS21280 at 2938.476 kb on - strand, within RPSI07_RS21280 at 2938.476 kb on - strand, within RPSI07_RS21280 at 2938.476 kb on - strand, within RPSI07_RS21280 at 2938.476 kb on - strand, within RPSI07_RS21280 at 2938.494 kb on + strand, within RPSI07_RS21280 at 2938.495 kb on - strand, within RPSI07_RS21280 at 2938.572 kb on + strand at 2938.573 kb on - strand at 2938.575 kb on + strand at 2938.662 kb on + strand at 2938.662 kb on + strand at 2938.663 kb on - strand at 2938.663 kb on - strand at 2938.663 kb on - strand at 2938.663 kb on - strand at 2938.986 kb on + strand, within RPSI07_RS21290 at 2939.134 kb on + strand at 2939.343 kb on + strand at 2939.344 kb on - strand at 2939.494 kb on + strand at 2939.495 kb on - strand at 2939.495 kb on - strand at 2939.495 kb on - strand at 2939.545 kb on + strand at 2939.545 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 2,937,781 - RPSI07_RS21280 0.47 +0.7 2,937,781 - RPSI07_RS21280 0.47 -0.3 2,937,781 - RPSI07_RS21280 0.47 -1.3 2,937,795 + RPSI07_RS21280 0.48 -0.6 2,937,795 + RPSI07_RS21280 0.48 -2.0 2,937,878 + RPSI07_RS21280 0.53 +1.0 2,937,969 + RPSI07_RS21280 0.58 -1.8 2,937,970 - RPSI07_RS21280 0.59 -1.9 2,937,970 - RPSI07_RS21280 0.59 -0.6 2,938,136 + RPSI07_RS21280 0.68 -0.7 2,938,137 - RPSI07_RS21280 0.68 +1.0 2,938,161 + RPSI07_RS21280 0.70 +3.6 2,938,161 + RPSI07_RS21280 0.70 +1.5 2,938,161 + RPSI07_RS21280 0.70 -2.7 2,938,161 + RPSI07_RS21280 0.70 -0.3 2,938,162 - RPSI07_RS21280 0.70 +0.4 2,938,162 - RPSI07_RS21280 0.70 -0.5 2,938,162 - RPSI07_RS21280 0.70 -0.0 2,938,162 - RPSI07_RS21280 0.70 -0.8 2,938,162 - RPSI07_RS21280 0.70 -0.3 2,938,162 - RPSI07_RS21280 0.70 -1.0 2,938,335 + RPSI07_RS21280 0.80 -0.3 2,938,335 + RPSI07_RS21280 0.80 +1.1 2,938,407 + RPSI07_RS21280 0.84 +1.0 2,938,408 - RPSI07_RS21280 0.84 +0.7 2,938,408 - RPSI07_RS21280 0.84 -0.6 2,938,408 - RPSI07_RS21280 0.84 +1.0 2,938,423 - RPSI07_RS21280 0.85 +1.0 2,938,423 - RPSI07_RS21280 0.85 +0.4 2,938,423 - RPSI07_RS21280 0.85 -0.3 2,938,423 - RPSI07_RS21280 0.85 +0.6 2,938,473 + RPSI07_RS21280 0.88 -0.7 2,938,475 + RPSI07_RS21280 0.88 +1.0 2,938,475 + RPSI07_RS21280 0.88 -1.1 2,938,476 - RPSI07_RS21280 0.88 -0.0 2,938,476 - RPSI07_RS21280 0.88 -0.5 2,938,476 - RPSI07_RS21280 0.88 -1.1 2,938,476 - RPSI07_RS21280 0.88 +0.4 2,938,494 + RPSI07_RS21280 0.89 +0.4 2,938,495 - RPSI07_RS21280 0.89 -2.6 2,938,572 + -1.0 2,938,573 - -1.0 2,938,575 + -2.6 2,938,662 + -0.9 2,938,662 + +0.1 2,938,663 - +0.3 2,938,663 - +0.1 2,938,663 - +0.2 2,938,663 - +0.4 2,938,986 + RPSI07_RS21290 0.30 -0.8 2,939,134 + +1.1 2,939,343 + +2.0 2,939,344 - -0.8 2,939,494 + -0.8 2,939,495 - +2.0 2,939,495 - -0.4 2,939,495 - -0.5 2,939,545 + +0.0 2,939,545 + -1.7
Or see this region's nucleotide sequence