Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21025 and RPSI07_RS21030 are separated by 78 nucleotides RPSI07_RS21030 and RPSI07_RS21035 are separated by 155 nucleotides
RPSI07_RS21025: RPSI07_RS21025 - TonB-dependent receptor, at 2,881,490 to 2,883,679
_RS21025
RPSI07_RS21030: RPSI07_RS21030 - hypothetical protein, at 2,883,758 to 2,884,135
_RS21030
RPSI07_RS21035: RPSI07_RS21035 - hypothetical protein, at 2,884,291 to 2,885,277
_RS21035
Position (kb)
2883
2884
2885 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2882.911 kb on - strand, within RPSI07_RS21025 at 2882.911 kb on - strand, within RPSI07_RS21025 at 2883.021 kb on + strand, within RPSI07_RS21025 at 2883.021 kb on + strand, within RPSI07_RS21025 at 2883.022 kb on - strand, within RPSI07_RS21025 at 2883.022 kb on - strand, within RPSI07_RS21025 at 2883.022 kb on - strand, within RPSI07_RS21025 at 2883.022 kb on - strand, within RPSI07_RS21025 at 2883.024 kb on + strand, within RPSI07_RS21025 at 2883.024 kb on + strand, within RPSI07_RS21025 at 2883.024 kb on + strand, within RPSI07_RS21025 at 2883.024 kb on + strand, within RPSI07_RS21025 at 2883.025 kb on - strand, within RPSI07_RS21025 at 2883.025 kb on - strand, within RPSI07_RS21025 at 2883.025 kb on - strand, within RPSI07_RS21025 at 2883.025 kb on - strand, within RPSI07_RS21025 at 2883.189 kb on + strand, within RPSI07_RS21025 at 2884.202 kb on + strand at 2884.790 kb on + strand, within RPSI07_RS21035 at 2884.790 kb on + strand, within RPSI07_RS21035 at 2884.790 kb on + strand, within RPSI07_RS21035 at 2884.791 kb on - strand, within RPSI07_RS21035 at 2884.791 kb on - strand, within RPSI07_RS21035 at 2884.817 kb on + strand, within RPSI07_RS21035 at 2884.818 kb on - strand, within RPSI07_RS21035 at 2884.818 kb on - strand, within RPSI07_RS21035
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 2,882,911 - RPSI07_RS21025 0.65 -1.6 2,882,911 - RPSI07_RS21025 0.65 +1.0 2,883,021 + RPSI07_RS21025 0.70 +2.6 2,883,021 + RPSI07_RS21025 0.70 -0.6 2,883,022 - RPSI07_RS21025 0.70 +1.3 2,883,022 - RPSI07_RS21025 0.70 -0.6 2,883,022 - RPSI07_RS21025 0.70 -0.0 2,883,022 - RPSI07_RS21025 0.70 -1.4 2,883,024 + RPSI07_RS21025 0.70 -0.6 2,883,024 + RPSI07_RS21025 0.70 -1.9 2,883,024 + RPSI07_RS21025 0.70 +0.5 2,883,024 + RPSI07_RS21025 0.70 -0.4 2,883,025 - RPSI07_RS21025 0.70 +2.0 2,883,025 - RPSI07_RS21025 0.70 -1.0 2,883,025 - RPSI07_RS21025 0.70 +1.0 2,883,025 - RPSI07_RS21025 0.70 -0.0 2,883,189 + RPSI07_RS21025 0.78 -0.0 2,884,202 + +0.5 2,884,790 + RPSI07_RS21035 0.51 -1.7 2,884,790 + RPSI07_RS21035 0.51 +0.1 2,884,790 + RPSI07_RS21035 0.51 -1.6 2,884,791 - RPSI07_RS21035 0.51 +0.7 2,884,791 - RPSI07_RS21035 0.51 +1.0 2,884,817 + RPSI07_RS21035 0.53 +0.1 2,884,818 - RPSI07_RS21035 0.53 -1.8 2,884,818 - RPSI07_RS21035 0.53 +1.4
Or see this region's nucleotide sequence