Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS20175 and RPSI07_RS20180 are separated by 159 nucleotides RPSI07_RS20180 and RPSI07_RS20185 are separated by 38 nucleotides RPSI07_RS20185 and RPSI07_RS20190 are separated by 49 nucleotides RPSI07_RS20190 and RPSI07_RS20195 are separated by 10 nucleotides
RPSI07_RS20175: RPSI07_RS20175 - Lrp/AsnC family transcriptional regulator, at 2,712,176 to 2,712,700
_RS20175
RPSI07_RS20180: RPSI07_RS20180 - arylformamidase, at 2,712,860 to 2,713,489
_RS20180
RPSI07_RS20185: RPSI07_RS20185 - kynureninase, at 2,713,528 to 2,714,781
_RS20185
RPSI07_RS20190: RPSI07_RS20190 - tryptophan 2,3-dioxygenase, at 2,714,831 to 2,715,667
_RS20190
RPSI07_RS20195: RPSI07_RS20195 - IclR family transcriptional regulator, at 2,715,678 to 2,716,499
_RS20195
Position (kb)
2713
2714
2715 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 2712.804 kb on - strand at 2713.082 kb on + strand, within RPSI07_RS20180 at 2713.191 kb on - strand, within RPSI07_RS20180 at 2713.410 kb on - strand, within RPSI07_RS20180 at 2713.622 kb on - strand at 2713.622 kb on - strand at 2713.622 kb on - strand at 2713.930 kb on - strand, within RPSI07_RS20185 at 2713.931 kb on - strand, within RPSI07_RS20185 at 2714.056 kb on + strand, within RPSI07_RS20185 at 2714.056 kb on + strand, within RPSI07_RS20185 at 2714.056 kb on + strand, within RPSI07_RS20185 at 2714.056 kb on + strand, within RPSI07_RS20185 at 2714.074 kb on + strand, within RPSI07_RS20185 at 2714.074 kb on + strand, within RPSI07_RS20185 at 2714.074 kb on + strand, within RPSI07_RS20185 at 2714.074 kb on + strand, within RPSI07_RS20185 at 2714.074 kb on + strand, within RPSI07_RS20185 at 2714.074 kb on + strand, within RPSI07_RS20185 at 2714.075 kb on - strand, within RPSI07_RS20185 at 2714.075 kb on - strand, within RPSI07_RS20185 at 2714.075 kb on - strand, within RPSI07_RS20185 at 2714.075 kb on - strand, within RPSI07_RS20185 at 2714.188 kb on + strand, within RPSI07_RS20185 at 2714.206 kb on + strand, within RPSI07_RS20185 at 2714.206 kb on + strand, within RPSI07_RS20185 at 2714.207 kb on - strand, within RPSI07_RS20185 at 2714.207 kb on - strand, within RPSI07_RS20185 at 2714.207 kb on - strand, within RPSI07_RS20185 at 2714.212 kb on + strand, within RPSI07_RS20185 at 2714.212 kb on + strand, within RPSI07_RS20185 at 2714.212 kb on + strand, within RPSI07_RS20185 at 2714.213 kb on - strand, within RPSI07_RS20185 at 2714.213 kb on - strand, within RPSI07_RS20185 at 2714.570 kb on - strand, within RPSI07_RS20185 at 2714.766 kb on + strand at 2714.766 kb on + strand at 2714.766 kb on + strand at 2714.766 kb on + strand at 2714.766 kb on + strand at 2714.767 kb on - strand at 2714.767 kb on - strand at 2714.767 kb on - strand at 2714.767 kb on - strand at 2714.894 kb on + strand at 2714.894 kb on + strand at 2714.894 kb on + strand at 2714.894 kb on + strand at 2714.894 kb on + strand at 2714.894 kb on + strand at 2714.895 kb on - strand at 2714.895 kb on - strand at 2714.904 kb on - strand at 2714.904 kb on - strand at 2714.904 kb on - strand at 2715.203 kb on + strand, within RPSI07_RS20190 at 2715.203 kb on + strand, within RPSI07_RS20190 at 2715.204 kb on - strand, within RPSI07_RS20190 at 2715.210 kb on - strand, within RPSI07_RS20190 at 2715.285 kb on - strand, within RPSI07_RS20190 at 2715.341 kb on + strand, within RPSI07_RS20190 at 2715.341 kb on + strand, within RPSI07_RS20190 at 2715.410 kb on + strand, within RPSI07_RS20190 at 2715.411 kb on - strand, within RPSI07_RS20190 at 2715.411 kb on - strand, within RPSI07_RS20190 at 2715.476 kb on + strand, within RPSI07_RS20190 at 2715.477 kb on - strand, within RPSI07_RS20190 at 2715.602 kb on + strand at 2715.602 kb on + strand at 2715.602 kb on + strand at 2715.774 kb on - strand, within RPSI07_RS20195
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 2,712,804 - -1.7 2,713,082 + RPSI07_RS20180 0.35 -0.7 2,713,191 - RPSI07_RS20180 0.53 -0.0 2,713,410 - RPSI07_RS20180 0.87 -0.1 2,713,622 - +0.3 2,713,622 - -0.5 2,713,622 - -0.7 2,713,930 - RPSI07_RS20185 0.32 -1.1 2,713,931 - RPSI07_RS20185 0.32 -0.8 2,714,056 + RPSI07_RS20185 0.42 -0.8 2,714,056 + RPSI07_RS20185 0.42 -0.2 2,714,056 + RPSI07_RS20185 0.42 -0.3 2,714,056 + RPSI07_RS20185 0.42 -1.4 2,714,074 + RPSI07_RS20185 0.44 -0.5 2,714,074 + RPSI07_RS20185 0.44 -0.3 2,714,074 + RPSI07_RS20185 0.44 -0.1 2,714,074 + RPSI07_RS20185 0.44 -2.1 2,714,074 + RPSI07_RS20185 0.44 -0.2 2,714,074 + RPSI07_RS20185 0.44 +0.8 2,714,075 - RPSI07_RS20185 0.44 +0.0 2,714,075 - RPSI07_RS20185 0.44 +0.6 2,714,075 - RPSI07_RS20185 0.44 -0.4 2,714,075 - RPSI07_RS20185 0.44 +0.0 2,714,188 + RPSI07_RS20185 0.53 -2.5 2,714,206 + RPSI07_RS20185 0.54 -0.1 2,714,206 + RPSI07_RS20185 0.54 -0.5 2,714,207 - RPSI07_RS20185 0.54 +0.9 2,714,207 - RPSI07_RS20185 0.54 -1.4 2,714,207 - RPSI07_RS20185 0.54 -0.6 2,714,212 + RPSI07_RS20185 0.55 -1.4 2,714,212 + RPSI07_RS20185 0.55 -0.4 2,714,212 + RPSI07_RS20185 0.55 +1.9 2,714,213 - RPSI07_RS20185 0.55 +0.8 2,714,213 - RPSI07_RS20185 0.55 -1.4 2,714,570 - RPSI07_RS20185 0.83 -0.1 2,714,766 + -1.1 2,714,766 + +1.5 2,714,766 + -0.5 2,714,766 + +0.1 2,714,766 + +0.9 2,714,767 - -1.1 2,714,767 - -0.5 2,714,767 - -0.3 2,714,767 - +2.3 2,714,894 + +2.5 2,714,894 + -0.7 2,714,894 + -4.1 2,714,894 + +0.6 2,714,894 + -0.7 2,714,894 + -0.2 2,714,895 - +0.3 2,714,895 - +0.9 2,714,904 - +0.9 2,714,904 - -0.3 2,714,904 - +1.7 2,715,203 + RPSI07_RS20190 0.44 -1.1 2,715,203 + RPSI07_RS20190 0.44 -1.8 2,715,204 - RPSI07_RS20190 0.45 +3.6 2,715,210 - RPSI07_RS20190 0.45 -1.0 2,715,285 - RPSI07_RS20190 0.54 +0.4 2,715,341 + RPSI07_RS20190 0.61 +0.2 2,715,341 + RPSI07_RS20190 0.61 +1.5 2,715,410 + RPSI07_RS20190 0.69 -0.5 2,715,411 - RPSI07_RS20190 0.69 -0.0 2,715,411 - RPSI07_RS20190 0.69 +5.2 2,715,476 + RPSI07_RS20190 0.77 -0.6 2,715,477 - RPSI07_RS20190 0.77 +1.8 2,715,602 + -0.8 2,715,602 + -0.5 2,715,602 + -0.4 2,715,774 - RPSI07_RS20195 0.12 +0.0
Or see this region's nucleotide sequence