Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS20170

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS20160 and RPSI07_RS20165 are separated by 210 nucleotidesRPSI07_RS20165 and RPSI07_RS20170 are separated by 9 nucleotidesRPSI07_RS20170 and RPSI07_RS20175 are separated by 82 nucleotidesRPSI07_RS20175 and RPSI07_RS20180 are separated by 159 nucleotides RPSI07_RS20160: RPSI07_RS20160 - peptide-methionine (S)-S-oxide reductase, at 2,710,540 to 2,711,100 _RS20160 RPSI07_RS20165: RPSI07_RS20165 - flavin reductase, at 2,711,311 to 2,711,877 _RS20165 RPSI07_RS20170: RPSI07_RS20170 - DUF3079 domain-containing protein, at 2,711,887 to 2,712,093 _RS20170 RPSI07_RS20175: RPSI07_RS20175 - Lrp/AsnC family transcriptional regulator, at 2,712,176 to 2,712,700 _RS20175 RPSI07_RS20180: RPSI07_RS20180 - arylformamidase, at 2,712,860 to 2,713,489 _RS20180 Position (kb) 2711 2712 2713Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2710.962 kb on - strand, within RPSI07_RS20160at 2710.991 kb on + strand, within RPSI07_RS20160at 2711.156 kb on + strandat 2711.157 kb on - strandat 2711.257 kb on - strandat 2711.325 kb on + strandat 2711.590 kb on + strand, within RPSI07_RS20165at 2711.590 kb on + strand, within RPSI07_RS20165at 2711.590 kb on + strand, within RPSI07_RS20165at 2711.590 kb on + strand, within RPSI07_RS20165at 2711.753 kb on - strand, within RPSI07_RS20165at 2711.753 kb on - strand, within RPSI07_RS20165at 2711.948 kb on + strand, within RPSI07_RS20170at 2711.949 kb on - strand, within RPSI07_RS20170at 2712.029 kb on + strand, within RPSI07_RS20170at 2712.029 kb on + strand, within RPSI07_RS20170at 2712.029 kb on + strand, within RPSI07_RS20170at 2712.030 kb on - strand, within RPSI07_RS20170at 2712.030 kb on - strand, within RPSI07_RS20170at 2712.030 kb on - strand, within RPSI07_RS20170at 2712.030 kb on - strand, within RPSI07_RS20170at 2712.030 kb on - strand, within RPSI07_RS20170at 2712.349 kb on - strand, within RPSI07_RS20175at 2712.384 kb on + strand, within RPSI07_RS20175at 2712.384 kb on + strand, within RPSI07_RS20175at 2712.384 kb on + strand, within RPSI07_RS20175at 2712.384 kb on + strand, within RPSI07_RS20175at 2712.384 kb on + strand, within RPSI07_RS20175at 2712.385 kb on - strand, within RPSI07_RS20175at 2712.408 kb on + strand, within RPSI07_RS20175at 2712.408 kb on + strand, within RPSI07_RS20175at 2712.408 kb on + strand, within RPSI07_RS20175at 2712.409 kb on - strand, within RPSI07_RS20175at 2712.409 kb on - strand, within RPSI07_RS20175at 2712.409 kb on - strand, within RPSI07_RS20175at 2712.409 kb on - strand, within RPSI07_RS20175at 2712.804 kb on - strandat 2713.082 kb on + strand, within RPSI07_RS20180

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
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2,710,962 - RPSI07_RS20160 0.75 +2.3
2,710,991 + RPSI07_RS20160 0.80 -1.7
2,711,156 + +0.5
2,711,157 - -0.5
2,711,257 - -0.3
2,711,325 + +3.7
2,711,590 + RPSI07_RS20165 0.49 +3.3
2,711,590 + RPSI07_RS20165 0.49 +2.5
2,711,590 + RPSI07_RS20165 0.49 -1.1
2,711,590 + RPSI07_RS20165 0.49 -1.3
2,711,753 - RPSI07_RS20165 0.78 +1.9
2,711,753 - RPSI07_RS20165 0.78 -0.6
2,711,948 + RPSI07_RS20170 0.29 -1.3
2,711,949 - RPSI07_RS20170 0.30 +0.2
2,712,029 + RPSI07_RS20170 0.69 +0.4
2,712,029 + RPSI07_RS20170 0.69 -0.2
2,712,029 + RPSI07_RS20170 0.69 +0.9
2,712,030 - RPSI07_RS20170 0.69 -0.1
2,712,030 - RPSI07_RS20170 0.69 +0.9
2,712,030 - RPSI07_RS20170 0.69 +1.5
2,712,030 - RPSI07_RS20170 0.69 +0.6
2,712,030 - RPSI07_RS20170 0.69 -0.2
2,712,349 - RPSI07_RS20175 0.33 -1.1
2,712,384 + RPSI07_RS20175 0.40 +1.3
2,712,384 + RPSI07_RS20175 0.40 +3.3
2,712,384 + RPSI07_RS20175 0.40 +0.2
2,712,384 + RPSI07_RS20175 0.40 +0.0
2,712,384 + RPSI07_RS20175 0.40 -0.6
2,712,385 - RPSI07_RS20175 0.40 +1.1
2,712,408 + RPSI07_RS20175 0.44 -0.4
2,712,408 + RPSI07_RS20175 0.44 +1.9
2,712,408 + RPSI07_RS20175 0.44 -0.7
2,712,409 - RPSI07_RS20175 0.44 -0.2
2,712,409 - RPSI07_RS20175 0.44 +2.9
2,712,409 - RPSI07_RS20175 0.44 +0.9
2,712,409 - RPSI07_RS20175 0.44 -1.8
2,712,804 - -1.7
2,713,082 + RPSI07_RS20180 0.35 -0.7

Or see this region's nucleotide sequence