Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS19665 and RPSI07_RS19670 are separated by 59 nucleotides RPSI07_RS19670 and RPSI07_RS19675 are separated by 3 nucleotides RPSI07_RS19675 and RPSI07_RS19680 are separated by 183 nucleotides RPSI07_RS19680 and RPSI07_RS19685 are separated by 36 nucleotides
RPSI07_RS19665: RPSI07_RS19665 - UDP-glucose/GDP-mannose dehydrogenase family protein, at 2,588,327 to 2,589,700
_RS19665
RPSI07_RS19670: RPSI07_RS19670 - lipopolysaccharide assembly protein LapB, at 2,589,760 to 2,591,034
_RS19670
RPSI07_RS19675: RPSI07_RS19675 - DUF1049 domain-containing protein, at 2,591,038 to 2,591,358
_RS19675
RPSI07_RS19680: RPSI07_RS19680 - integration host factor subunit beta, at 2,591,542 to 2,591,964
_RS19680
RPSI07_RS19685: RPSI07_RS19685 - 30S ribosomal protein S1, at 2,592,001 to 2,593,689
_RS19685
Position (kb)
2589
2590
2591
2592 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2589.762 kb on - strand at 2589.762 kb on - strand at 2589.762 kb on - strand at 2589.762 kb on - strand at 2589.762 kb on - strand at 2589.762 kb on - strand at 2589.762 kb on - strand at 2589.762 kb on - strand at 2589.781 kb on - strand at 2591.119 kb on - strand, within RPSI07_RS19675 at 2591.119 kb on - strand, within RPSI07_RS19675 at 2592.002 kb on + strand at 2592.003 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 2,589,762 - +1.5 2,589,762 - +0.1 2,589,762 - +0.7 2,589,762 - -0.8 2,589,762 - +2.9 2,589,762 - +0.7 2,589,762 - -0.8 2,589,762 - +2.0 2,589,781 - -0.6 2,591,119 - RPSI07_RS19675 0.25 -2.4 2,591,119 - RPSI07_RS19675 0.25 +1.5 2,592,002 + -1.7 2,592,003 - -0.5
Or see this region's nucleotide sequence