Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS19670

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS19665 and RPSI07_RS19670 are separated by 59 nucleotidesRPSI07_RS19670 and RPSI07_RS19675 are separated by 3 nucleotidesRPSI07_RS19675 and RPSI07_RS19680 are separated by 183 nucleotidesRPSI07_RS19680 and RPSI07_RS19685 are separated by 36 nucleotides RPSI07_RS19665: RPSI07_RS19665 - UDP-glucose/GDP-mannose dehydrogenase family protein, at 2,588,327 to 2,589,700 _RS19665 RPSI07_RS19670: RPSI07_RS19670 - lipopolysaccharide assembly protein LapB, at 2,589,760 to 2,591,034 _RS19670 RPSI07_RS19675: RPSI07_RS19675 - DUF1049 domain-containing protein, at 2,591,038 to 2,591,358 _RS19675 RPSI07_RS19680: RPSI07_RS19680 - integration host factor subunit beta, at 2,591,542 to 2,591,964 _RS19680 RPSI07_RS19685: RPSI07_RS19685 - 30S ribosomal protein S1, at 2,592,001 to 2,593,689 _RS19685 Position (kb) 2589 2590 2591 2592Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2589.762 kb on - strandat 2589.762 kb on - strandat 2589.762 kb on - strandat 2589.762 kb on - strandat 2589.762 kb on - strandat 2589.762 kb on - strandat 2589.762 kb on - strandat 2589.762 kb on - strandat 2589.781 kb on - strandat 2591.119 kb on - strand, within RPSI07_RS19675at 2591.119 kb on - strand, within RPSI07_RS19675at 2592.002 kb on + strandat 2592.003 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
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2,589,762 - +1.5
2,589,762 - +0.1
2,589,762 - +0.7
2,589,762 - -0.8
2,589,762 - +2.9
2,589,762 - +0.7
2,589,762 - -0.8
2,589,762 - +2.0
2,589,781 - -0.6
2,591,119 - RPSI07_RS19675 0.25 -2.4
2,591,119 - RPSI07_RS19675 0.25 +1.5
2,592,002 + -1.7
2,592,003 - -0.5

Or see this region's nucleotide sequence