Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS19495

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS19490 and RPSI07_RS19495 are separated by 208 nucleotidesRPSI07_RS19495 and RPSI07_RS19500 overlap by 4 nucleotidesRPSI07_RS19500 and RPSI07_RS19505 are separated by 73 nucleotides RPSI07_RS19490: RPSI07_RS19490 - serine/threonine dehydratase, at 2,557,466 to 2,558,470 _RS19490 RPSI07_RS19495: RPSI07_RS19495 - thymidylate synthase, at 2,558,679 to 2,559,473 _RS19495 RPSI07_RS19500: RPSI07_RS19500 - dihydrofolate reductase, at 2,559,470 to 2,559,973 _RS19500 RPSI07_RS19505: RPSI07_RS19505 - metalloprotease PmbA, at 2,560,047 to 2,561,420 _RS19505 Position (kb) 2558 2559 2560Strain fitness (log2 ratio) -1 0 1 2 3at 2557.803 kb on + strand, within RPSI07_RS19490at 2557.803 kb on + strand, within RPSI07_RS19490at 2557.809 kb on + strand, within RPSI07_RS19490at 2557.810 kb on - strand, within RPSI07_RS19490at 2557.810 kb on - strand, within RPSI07_RS19490at 2557.810 kb on - strand, within RPSI07_RS19490at 2557.810 kb on - strand, within RPSI07_RS19490at 2557.810 kb on - strand, within RPSI07_RS19490at 2557.917 kb on + strand, within RPSI07_RS19490at 2557.918 kb on - strand, within RPSI07_RS19490at 2557.918 kb on - strand, within RPSI07_RS19490at 2557.918 kb on - strand, within RPSI07_RS19490at 2557.962 kb on + strand, within RPSI07_RS19490at 2558.256 kb on + strand, within RPSI07_RS19490at 2558.256 kb on + strand, within RPSI07_RS19490at 2558.257 kb on - strand, within RPSI07_RS19490at 2558.257 kb on - strand, within RPSI07_RS19490at 2558.262 kb on + strand, within RPSI07_RS19490at 2558.302 kb on + strand, within RPSI07_RS19490at 2558.302 kb on + strand, within RPSI07_RS19490at 2558.302 kb on + strand, within RPSI07_RS19490at 2558.364 kb on + strand, within RPSI07_RS19490at 2558.364 kb on + strand, within RPSI07_RS19490at 2558.639 kb on + strandat 2558.640 kb on - strandat 2560.237 kb on + strand, within RPSI07_RS19505at 2560.238 kb on - strand, within RPSI07_RS19505at 2560.252 kb on + strand, within RPSI07_RS19505at 2560.252 kb on + strand, within RPSI07_RS19505at 2560.253 kb on - strand, within RPSI07_RS19505at 2560.253 kb on - strand, within RPSI07_RS19505at 2560.418 kb on - strand, within RPSI07_RS19505at 2560.433 kb on - strand, within RPSI07_RS19505

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
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2,557,803 + RPSI07_RS19490 0.34 -0.5
2,557,803 + RPSI07_RS19490 0.34 -0.9
2,557,809 + RPSI07_RS19490 0.34 -0.7
2,557,810 - RPSI07_RS19490 0.34 -0.1
2,557,810 - RPSI07_RS19490 0.34 +0.1
2,557,810 - RPSI07_RS19490 0.34 +2.2
2,557,810 - RPSI07_RS19490 0.34 -1.1
2,557,810 - RPSI07_RS19490 0.34 +0.3
2,557,917 + RPSI07_RS19490 0.45 -0.8
2,557,918 - RPSI07_RS19490 0.45 -0.3
2,557,918 - RPSI07_RS19490 0.45 -0.4
2,557,918 - RPSI07_RS19490 0.45 -1.7
2,557,962 + RPSI07_RS19490 0.49 +1.0
2,558,256 + RPSI07_RS19490 0.79 +0.6
2,558,256 + RPSI07_RS19490 0.79 +2.9
2,558,257 - RPSI07_RS19490 0.79 +1.4
2,558,257 - RPSI07_RS19490 0.79 +0.0
2,558,262 + RPSI07_RS19490 0.79 +1.8
2,558,302 + RPSI07_RS19490 0.83 +0.9
2,558,302 + RPSI07_RS19490 0.83 -0.2
2,558,302 + RPSI07_RS19490 0.83 +2.5
2,558,364 + RPSI07_RS19490 0.89 -0.7
2,558,364 + RPSI07_RS19490 0.89 -1.6
2,558,639 + +2.9
2,558,640 - +0.5
2,560,237 + RPSI07_RS19505 0.14 +0.3
2,560,238 - RPSI07_RS19505 0.14 +0.9
2,560,252 + RPSI07_RS19505 0.15 +0.9
2,560,252 + RPSI07_RS19505 0.15 -0.5
2,560,253 - RPSI07_RS19505 0.15 -0.9
2,560,253 - RPSI07_RS19505 0.15 -1.1
2,560,418 - RPSI07_RS19505 0.27 +0.1
2,560,433 - RPSI07_RS19505 0.28 -0.4

Or see this region's nucleotide sequence