Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS19490 and RPSI07_RS19495 are separated by 208 nucleotides RPSI07_RS19495 and RPSI07_RS19500 overlap by 4 nucleotides RPSI07_RS19500 and RPSI07_RS19505 are separated by 73 nucleotides
RPSI07_RS19490: RPSI07_RS19490 - serine/threonine dehydratase, at 2,557,466 to 2,558,470
_RS19490
RPSI07_RS19495: RPSI07_RS19495 - thymidylate synthase, at 2,558,679 to 2,559,473
_RS19495
RPSI07_RS19500: RPSI07_RS19500 - dihydrofolate reductase, at 2,559,470 to 2,559,973
_RS19500
RPSI07_RS19505: RPSI07_RS19505 - metalloprotease PmbA, at 2,560,047 to 2,561,420
_RS19505
Position (kb)
2558
2559
2560 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2557.803 kb on + strand, within RPSI07_RS19490 at 2557.803 kb on + strand, within RPSI07_RS19490 at 2557.809 kb on + strand, within RPSI07_RS19490 at 2557.810 kb on - strand, within RPSI07_RS19490 at 2557.810 kb on - strand, within RPSI07_RS19490 at 2557.810 kb on - strand, within RPSI07_RS19490 at 2557.810 kb on - strand, within RPSI07_RS19490 at 2557.810 kb on - strand, within RPSI07_RS19490 at 2557.917 kb on + strand, within RPSI07_RS19490 at 2557.918 kb on - strand, within RPSI07_RS19490 at 2557.918 kb on - strand, within RPSI07_RS19490 at 2557.918 kb on - strand, within RPSI07_RS19490 at 2557.962 kb on + strand, within RPSI07_RS19490 at 2558.256 kb on + strand, within RPSI07_RS19490 at 2558.256 kb on + strand, within RPSI07_RS19490 at 2558.257 kb on - strand, within RPSI07_RS19490 at 2558.257 kb on - strand, within RPSI07_RS19490 at 2558.262 kb on + strand, within RPSI07_RS19490 at 2558.302 kb on + strand, within RPSI07_RS19490 at 2558.302 kb on + strand, within RPSI07_RS19490 at 2558.302 kb on + strand, within RPSI07_RS19490 at 2558.364 kb on + strand, within RPSI07_RS19490 at 2558.364 kb on + strand, within RPSI07_RS19490 at 2558.639 kb on + strand at 2558.640 kb on - strand at 2560.237 kb on + strand, within RPSI07_RS19505 at 2560.238 kb on - strand, within RPSI07_RS19505 at 2560.252 kb on + strand, within RPSI07_RS19505 at 2560.252 kb on + strand, within RPSI07_RS19505 at 2560.253 kb on - strand, within RPSI07_RS19505 at 2560.253 kb on - strand, within RPSI07_RS19505 at 2560.418 kb on - strand, within RPSI07_RS19505 at 2560.433 kb on - strand, within RPSI07_RS19505
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 2,557,803 + RPSI07_RS19490 0.34 -0.5 2,557,803 + RPSI07_RS19490 0.34 -0.9 2,557,809 + RPSI07_RS19490 0.34 -0.7 2,557,810 - RPSI07_RS19490 0.34 -0.1 2,557,810 - RPSI07_RS19490 0.34 +0.1 2,557,810 - RPSI07_RS19490 0.34 +2.2 2,557,810 - RPSI07_RS19490 0.34 -1.1 2,557,810 - RPSI07_RS19490 0.34 +0.3 2,557,917 + RPSI07_RS19490 0.45 -0.8 2,557,918 - RPSI07_RS19490 0.45 -0.3 2,557,918 - RPSI07_RS19490 0.45 -0.4 2,557,918 - RPSI07_RS19490 0.45 -1.7 2,557,962 + RPSI07_RS19490 0.49 +1.0 2,558,256 + RPSI07_RS19490 0.79 +0.6 2,558,256 + RPSI07_RS19490 0.79 +2.9 2,558,257 - RPSI07_RS19490 0.79 +1.4 2,558,257 - RPSI07_RS19490 0.79 +0.0 2,558,262 + RPSI07_RS19490 0.79 +1.8 2,558,302 + RPSI07_RS19490 0.83 +0.9 2,558,302 + RPSI07_RS19490 0.83 -0.2 2,558,302 + RPSI07_RS19490 0.83 +2.5 2,558,364 + RPSI07_RS19490 0.89 -0.7 2,558,364 + RPSI07_RS19490 0.89 -1.6 2,558,639 + +2.9 2,558,640 - +0.5 2,560,237 + RPSI07_RS19505 0.14 +0.3 2,560,238 - RPSI07_RS19505 0.14 +0.9 2,560,252 + RPSI07_RS19505 0.15 +0.9 2,560,252 + RPSI07_RS19505 0.15 -0.5 2,560,253 - RPSI07_RS19505 0.15 -0.9 2,560,253 - RPSI07_RS19505 0.15 -1.1 2,560,418 - RPSI07_RS19505 0.27 +0.1 2,560,433 - RPSI07_RS19505 0.28 -0.4
Or see this region's nucleotide sequence