Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS17650 and RPSI07_RS17655 are separated by 41 nucleotides RPSI07_RS17655 and RPSI07_RS17660 are separated by 3 nucleotides
RPSI07_RS17650: RPSI07_RS17650 - RND transporter, at 2,161,839 to 2,163,386
_RS17650
RPSI07_RS17655: RPSI07_RS17655 - hemolysin D, at 2,163,428 to 2,164,708
_RS17655
RPSI07_RS17660: RPSI07_RS17660 - MFS transporter, at 2,164,712 to 2,166,268
_RS17660
Position (kb)
2163
2164
2165 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2162.466 kb on + strand, within RPSI07_RS17650 at 2162.467 kb on - strand, within RPSI07_RS17650 at 2162.555 kb on + strand, within RPSI07_RS17650 at 2163.028 kb on - strand, within RPSI07_RS17650 at 2163.145 kb on - strand, within RPSI07_RS17650 at 2163.424 kb on + strand at 2163.575 kb on + strand, within RPSI07_RS17655 at 2163.588 kb on - strand, within RPSI07_RS17655 at 2163.588 kb on - strand, within RPSI07_RS17655 at 2163.588 kb on - strand, within RPSI07_RS17655 at 2163.620 kb on + strand, within RPSI07_RS17655 at 2163.620 kb on + strand, within RPSI07_RS17655 at 2163.620 kb on + strand, within RPSI07_RS17655 at 2163.620 kb on + strand, within RPSI07_RS17655 at 2163.621 kb on - strand, within RPSI07_RS17655 at 2164.091 kb on + strand, within RPSI07_RS17655 at 2164.091 kb on + strand, within RPSI07_RS17655 at 2164.127 kb on + strand, within RPSI07_RS17655 at 2164.127 kb on + strand, within RPSI07_RS17655 at 2164.127 kb on + strand, within RPSI07_RS17655 at 2164.127 kb on + strand, within RPSI07_RS17655 at 2164.127 kb on + strand, within RPSI07_RS17655 at 2164.128 kb on - strand, within RPSI07_RS17655 at 2164.290 kb on - strand, within RPSI07_RS17655 at 2164.307 kb on + strand, within RPSI07_RS17655 at 2164.307 kb on + strand, within RPSI07_RS17655 at 2164.308 kb on - strand, within RPSI07_RS17655 at 2164.308 kb on - strand, within RPSI07_RS17655 at 2164.560 kb on - strand, within RPSI07_RS17655 at 2164.813 kb on + strand at 2164.814 kb on - strand at 2165.240 kb on + strand, within RPSI07_RS17660 at 2165.240 kb on + strand, within RPSI07_RS17660 at 2165.240 kb on + strand, within RPSI07_RS17660 at 2165.241 kb on - strand, within RPSI07_RS17660 at 2165.241 kb on - strand, within RPSI07_RS17660 at 2165.241 kb on - strand, within RPSI07_RS17660 at 2165.274 kb on - strand, within RPSI07_RS17660 at 2165.292 kb on - strand, within RPSI07_RS17660 at 2165.292 kb on - strand, within RPSI07_RS17660 at 2165.292 kb on - strand, within RPSI07_RS17660 at 2165.568 kb on - strand, within RPSI07_RS17660 at 2165.628 kb on - strand, within RPSI07_RS17660
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 2,162,466 + RPSI07_RS17650 0.41 -1.5 2,162,467 - RPSI07_RS17650 0.41 +2.4 2,162,555 + RPSI07_RS17650 0.46 -0.8 2,163,028 - RPSI07_RS17650 0.77 -0.6 2,163,145 - RPSI07_RS17650 0.84 +2.9 2,163,424 + -0.1 2,163,575 + RPSI07_RS17655 0.11 -1.4 2,163,588 - RPSI07_RS17655 0.12 -1.1 2,163,588 - RPSI07_RS17655 0.12 -2.2 2,163,588 - RPSI07_RS17655 0.12 +0.6 2,163,620 + RPSI07_RS17655 0.15 -0.2 2,163,620 + RPSI07_RS17655 0.15 +0.2 2,163,620 + RPSI07_RS17655 0.15 +0.0 2,163,620 + RPSI07_RS17655 0.15 +0.4 2,163,621 - RPSI07_RS17655 0.15 -0.1 2,164,091 + RPSI07_RS17655 0.52 -2.1 2,164,091 + RPSI07_RS17655 0.52 +2.9 2,164,127 + RPSI07_RS17655 0.55 +0.5 2,164,127 + RPSI07_RS17655 0.55 -0.7 2,164,127 + RPSI07_RS17655 0.55 -1.3 2,164,127 + RPSI07_RS17655 0.55 -0.2 2,164,127 + RPSI07_RS17655 0.55 -0.2 2,164,128 - RPSI07_RS17655 0.55 +0.0 2,164,290 - RPSI07_RS17655 0.67 -1.4 2,164,307 + RPSI07_RS17655 0.69 -0.6 2,164,307 + RPSI07_RS17655 0.69 +0.4 2,164,308 - RPSI07_RS17655 0.69 -3.1 2,164,308 - RPSI07_RS17655 0.69 -0.9 2,164,560 - RPSI07_RS17655 0.88 -2.1 2,164,813 + -0.6 2,164,814 - +0.3 2,165,240 + RPSI07_RS17660 0.34 +0.3 2,165,240 + RPSI07_RS17660 0.34 -1.0 2,165,240 + RPSI07_RS17660 0.34 -0.5 2,165,241 - RPSI07_RS17660 0.34 -1.1 2,165,241 - RPSI07_RS17660 0.34 -0.1 2,165,241 - RPSI07_RS17660 0.34 -0.2 2,165,274 - RPSI07_RS17660 0.36 +1.7 2,165,292 - RPSI07_RS17660 0.37 +2.0 2,165,292 - RPSI07_RS17660 0.37 -1.4 2,165,292 - RPSI07_RS17660 0.37 -2.0 2,165,568 - RPSI07_RS17660 0.55 +0.9 2,165,628 - RPSI07_RS17660 0.59 +1.3
Or see this region's nucleotide sequence