Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS16975 and RPSI07_RS16980 are separated by 141 nucleotides RPSI07_RS16980 and RPSI07_RS16985 are separated by 154 nucleotides RPSI07_RS16985 and RPSI07_RS16990 are separated by 5 nucleotides
RPSI07_RS16975: RPSI07_RS16975 - IMP dehydrogenase, at 2,010,939 to 2,012,402
_RS16975
RPSI07_RS16980: RPSI07_RS16980 - EamA/RhaT family transporter, at 2,012,544 to 2,013,482
_RS16980
RPSI07_RS16985: RPSI07_RS16985 - DUF4124 domain-containing protein, at 2,013,637 to 2,014,173
_RS16985
RPSI07_RS16990: RPSI07_RS16990 - hypothetical protein, at 2,014,179 to 2,014,535
_RS16990
Position (kb)
2012
2013
2014 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2012.469 kb on - strand at 2012.660 kb on - strand, within RPSI07_RS16980 at 2012.664 kb on + strand, within RPSI07_RS16980 at 2012.664 kb on + strand, within RPSI07_RS16980 at 2012.779 kb on - strand, within RPSI07_RS16980 at 2012.781 kb on + strand, within RPSI07_RS16980 at 2012.781 kb on + strand, within RPSI07_RS16980 at 2012.782 kb on - strand, within RPSI07_RS16980 at 2012.782 kb on - strand, within RPSI07_RS16980 at 2012.920 kb on + strand, within RPSI07_RS16980 at 2012.923 kb on + strand, within RPSI07_RS16980 at 2012.924 kb on - strand, within RPSI07_RS16980 at 2013.060 kb on + strand, within RPSI07_RS16980 at 2013.061 kb on - strand, within RPSI07_RS16980 at 2013.061 kb on - strand, within RPSI07_RS16980 at 2013.061 kb on - strand, within RPSI07_RS16980 at 2013.378 kb on + strand, within RPSI07_RS16980 at 2013.534 kb on + strand at 2013.535 kb on - strand at 2013.666 kb on + strand at 2013.749 kb on - strand, within RPSI07_RS16985 at 2013.826 kb on + strand, within RPSI07_RS16985 at 2013.827 kb on - strand, within RPSI07_RS16985 at 2014.102 kb on + strand, within RPSI07_RS16985 at 2014.102 kb on + strand, within RPSI07_RS16985 at 2014.102 kb on + strand, within RPSI07_RS16985 at 2014.103 kb on - strand, within RPSI07_RS16985 at 2014.103 kb on - strand, within RPSI07_RS16985 at 2014.104 kb on + strand, within RPSI07_RS16985 at 2014.104 kb on + strand, within RPSI07_RS16985 at 2014.104 kb on + strand, within RPSI07_RS16985 at 2014.105 kb on - strand, within RPSI07_RS16985
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 2,012,469 - -0.4 2,012,660 - RPSI07_RS16980 0.12 -0.4 2,012,664 + RPSI07_RS16980 0.13 -0.8 2,012,664 + RPSI07_RS16980 0.13 -2.2 2,012,779 - RPSI07_RS16980 0.25 +1.8 2,012,781 + RPSI07_RS16980 0.25 -1.3 2,012,781 + RPSI07_RS16980 0.25 +2.4 2,012,782 - RPSI07_RS16980 0.25 -1.3 2,012,782 - RPSI07_RS16980 0.25 +0.7 2,012,920 + RPSI07_RS16980 0.40 +1.5 2,012,923 + RPSI07_RS16980 0.40 -0.5 2,012,924 - RPSI07_RS16980 0.40 -0.8 2,013,060 + RPSI07_RS16980 0.55 +0.8 2,013,061 - RPSI07_RS16980 0.55 +0.8 2,013,061 - RPSI07_RS16980 0.55 -0.8 2,013,061 - RPSI07_RS16980 0.55 +0.4 2,013,378 + RPSI07_RS16980 0.89 -0.8 2,013,534 + +1.0 2,013,535 - -1.4 2,013,666 + -0.4 2,013,749 - RPSI07_RS16985 0.21 +0.8 2,013,826 + RPSI07_RS16985 0.35 -0.4 2,013,827 - RPSI07_RS16985 0.35 -0.2 2,014,102 + RPSI07_RS16985 0.87 +0.8 2,014,102 + RPSI07_RS16985 0.87 +1.5 2,014,102 + RPSI07_RS16985 0.87 -2.8 2,014,103 - RPSI07_RS16985 0.87 +0.8 2,014,103 - RPSI07_RS16985 0.87 +1.2 2,014,104 + RPSI07_RS16985 0.87 +0.8 2,014,104 + RPSI07_RS16985 0.87 +0.2 2,014,104 + RPSI07_RS16985 0.87 -1.0 2,014,105 - RPSI07_RS16985 0.87 +0.3
Or see this region's nucleotide sequence