Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS15335 and RPSI07_RS15340 are separated by 76 nucleotides RPSI07_RS15340 and RPSI07_RS15345 are separated by 134 nucleotides RPSI07_RS15345 and RPSI07_RS15350 are separated by 140 nucleotides RPSI07_RS15350 and RPSI07_RS15355 are separated by 65 nucleotides
RPSI07_RS15335: RPSI07_RS15335 - exopolyphosphatase, at 1,604,078 to 1,605,616
_RS15335
RPSI07_RS15340: RPSI07_RS15340 - GNAT family N-acetyltransferase, at 1,605,693 to 1,606,529
_RS15340
RPSI07_RS15345: RPSI07_RS15345 - phosphohistidine phosphatase SixA, at 1,606,664 to 1,607,137
_RS15345
RPSI07_RS15350: RPSI07_RS15350 - tRNA-Pro, at 1,607,278 to 1,607,354
_RS15350
RPSI07_RS15355: RPSI07_RS15355 - sulfite exporter TauE/SafE family protein, at 1,607,420 to 1,608,241
_RS15355
Position (kb)
1605
1606
1607 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1604.734 kb on - strand, within RPSI07_RS15335 at 1604.866 kb on + strand, within RPSI07_RS15335 at 1604.866 kb on + strand, within RPSI07_RS15335 at 1604.867 kb on - strand, within RPSI07_RS15335 at 1605.011 kb on + strand, within RPSI07_RS15335 at 1605.011 kb on + strand, within RPSI07_RS15335 at 1605.011 kb on + strand, within RPSI07_RS15335 at 1605.011 kb on + strand, within RPSI07_RS15335 at 1605.011 kb on + strand, within RPSI07_RS15335 at 1605.011 kb on + strand, within RPSI07_RS15335 at 1605.011 kb on + strand, within RPSI07_RS15335 at 1605.012 kb on - strand, within RPSI07_RS15335 at 1605.012 kb on - strand, within RPSI07_RS15335 at 1605.012 kb on - strand, within RPSI07_RS15335 at 1605.243 kb on - strand, within RPSI07_RS15335 at 1605.243 kb on - strand, within RPSI07_RS15335 at 1605.260 kb on + strand, within RPSI07_RS15335 at 1605.260 kb on + strand, within RPSI07_RS15335 at 1605.260 kb on + strand, within RPSI07_RS15335 at 1605.260 kb on + strand, within RPSI07_RS15335 at 1605.260 kb on + strand, within RPSI07_RS15335 at 1605.260 kb on + strand, within RPSI07_RS15335 at 1605.261 kb on - strand, within RPSI07_RS15335 at 1605.261 kb on - strand, within RPSI07_RS15335 at 1605.261 kb on - strand, within RPSI07_RS15335 at 1605.261 kb on - strand, within RPSI07_RS15335 at 1605.263 kb on - strand, within RPSI07_RS15335 at 1605.263 kb on - strand, within RPSI07_RS15335 at 1605.263 kb on - strand, within RPSI07_RS15335 at 1605.263 kb on - strand, within RPSI07_RS15335 at 1605.421 kb on - strand, within RPSI07_RS15335 at 1605.421 kb on - strand, within RPSI07_RS15335 at 1605.548 kb on + strand at 1605.548 kb on + strand at 1605.549 kb on - strand at 1605.596 kb on + strand at 1605.596 kb on + strand at 1605.597 kb on - strand at 1605.823 kb on + strand, within RPSI07_RS15340 at 1605.824 kb on - strand, within RPSI07_RS15340 at 1606.033 kb on + strand, within RPSI07_RS15340 at 1606.033 kb on + strand, within RPSI07_RS15340 at 1606.213 kb on + strand, within RPSI07_RS15340 at 1606.213 kb on + strand, within RPSI07_RS15340 at 1606.213 kb on + strand, within RPSI07_RS15340 at 1606.214 kb on - strand, within RPSI07_RS15340 at 1606.345 kb on + strand, within RPSI07_RS15340 at 1606.345 kb on + strand, within RPSI07_RS15340 at 1606.345 kb on + strand, within RPSI07_RS15340 at 1606.345 kb on + strand, within RPSI07_RS15340 at 1606.345 kb on + strand, within RPSI07_RS15340 at 1606.346 kb on - strand, within RPSI07_RS15340 at 1606.346 kb on - strand, within RPSI07_RS15340 at 1606.346 kb on - strand, within RPSI07_RS15340 at 1606.346 kb on - strand, within RPSI07_RS15340 at 1606.346 kb on - strand, within RPSI07_RS15340 at 1606.566 kb on - strand at 1607.143 kb on + strand at 1607.143 kb on + strand at 1607.144 kb on - strand at 1607.149 kb on + strand at 1607.149 kb on + strand at 1607.149 kb on + strand at 1607.150 kb on - strand at 1607.150 kb on - strand at 1607.173 kb on - strand at 1607.174 kb on + strand at 1607.174 kb on + strand at 1607.175 kb on - strand at 1607.175 kb on - strand at 1607.175 kb on - strand at 1607.175 kb on - strand at 1607.175 kb on - strand at 1607.175 kb on - strand at 1607.265 kb on - strand at 1607.265 kb on - strand at 1607.368 kb on - strand at 1607.382 kb on - strand at 1607.382 kb on - strand at 1607.395 kb on + strand at 1607.396 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 1,604,734 - RPSI07_RS15335 0.43 +1.8 1,604,866 + RPSI07_RS15335 0.51 -1.5 1,604,866 + RPSI07_RS15335 0.51 -1.2 1,604,867 - RPSI07_RS15335 0.51 -2.6 1,605,011 + RPSI07_RS15335 0.61 +0.8 1,605,011 + RPSI07_RS15335 0.61 +0.4 1,605,011 + RPSI07_RS15335 0.61 -0.8 1,605,011 + RPSI07_RS15335 0.61 -1.1 1,605,011 + RPSI07_RS15335 0.61 -1.1 1,605,011 + RPSI07_RS15335 0.61 +0.8 1,605,011 + RPSI07_RS15335 0.61 +3.4 1,605,012 - RPSI07_RS15335 0.61 -1.3 1,605,012 - RPSI07_RS15335 0.61 -3.1 1,605,012 - RPSI07_RS15335 0.61 +0.2 1,605,243 - RPSI07_RS15335 0.76 +1.4 1,605,243 - RPSI07_RS15335 0.76 -0.7 1,605,260 + RPSI07_RS15335 0.77 -1.1 1,605,260 + RPSI07_RS15335 0.77 -1.5 1,605,260 + RPSI07_RS15335 0.77 -0.5 1,605,260 + RPSI07_RS15335 0.77 -0.5 1,605,260 + RPSI07_RS15335 0.77 -2.5 1,605,260 + RPSI07_RS15335 0.77 -1.8 1,605,261 - RPSI07_RS15335 0.77 -0.1 1,605,261 - RPSI07_RS15335 0.77 -0.8 1,605,261 - RPSI07_RS15335 0.77 -0.8 1,605,261 - RPSI07_RS15335 0.77 -0.5 1,605,263 - RPSI07_RS15335 0.77 -1.1 1,605,263 - RPSI07_RS15335 0.77 -1.2 1,605,263 - RPSI07_RS15335 0.77 +0.3 1,605,263 - RPSI07_RS15335 0.77 +0.3 1,605,421 - RPSI07_RS15335 0.87 -1.4 1,605,421 - RPSI07_RS15335 0.87 -2.3 1,605,548 + -0.4 1,605,548 + -1.1 1,605,549 - -1.0 1,605,596 + -0.2 1,605,596 + -1.5 1,605,597 - -1.6 1,605,823 + RPSI07_RS15340 0.16 +0.6 1,605,824 - RPSI07_RS15340 0.16 -1.1 1,606,033 + RPSI07_RS15340 0.41 -0.3 1,606,033 + RPSI07_RS15340 0.41 +0.1 1,606,213 + RPSI07_RS15340 0.62 +2.8 1,606,213 + RPSI07_RS15340 0.62 +3.1 1,606,213 + RPSI07_RS15340 0.62 +0.1 1,606,214 - RPSI07_RS15340 0.62 +2.1 1,606,345 + RPSI07_RS15340 0.78 +2.6 1,606,345 + RPSI07_RS15340 0.78 -2.5 1,606,345 + RPSI07_RS15340 0.78 -1.4 1,606,345 + RPSI07_RS15340 0.78 +1.4 1,606,345 + RPSI07_RS15340 0.78 +0.3 1,606,346 - RPSI07_RS15340 0.78 -0.9 1,606,346 - RPSI07_RS15340 0.78 +1.1 1,606,346 - RPSI07_RS15340 0.78 +1.7 1,606,346 - RPSI07_RS15340 0.78 +0.9 1,606,346 - RPSI07_RS15340 0.78 +0.5 1,606,566 - -0.4 1,607,143 + +0.4 1,607,143 + -2.2 1,607,144 - -0.6 1,607,149 + -0.6 1,607,149 + -1.2 1,607,149 + -1.0 1,607,150 - +0.1 1,607,150 - -3.5 1,607,173 - -0.2 1,607,174 + +1.6 1,607,174 + +0.4 1,607,175 - -1.2 1,607,175 - -1.4 1,607,175 - -1.8 1,607,175 - +0.4 1,607,175 - +0.0 1,607,175 - +0.0 1,607,265 - -3.0 1,607,265 - +0.1 1,607,368 - +3.8 1,607,382 - -1.5 1,607,382 - +2.4 1,607,395 + -0.4 1,607,396 - -0.2
Or see this region's nucleotide sequence