Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS11175 and RPSI07_RS11180 are separated by 27 nucleotides RPSI07_RS11180 and RPSI07_RS11185 are separated by 141 nucleotides RPSI07_RS11185 and RPSI07_RS11190 are separated by 38 nucleotides
RPSI07_RS11175: RPSI07_RS11175 - DUF2817 domain-containing protein, at 708,686 to 709,768
_RS11175
RPSI07_RS11180: RPSI07_RS11180 - EamA family transporter, at 709,796 to 710,695
_RS11180
RPSI07_RS11185: RPSI07_RS11185 - Lrp/AsnC family transcriptional regulator, at 710,837 to 711,313
_RS11185
RPSI07_RS11190: RPSI07_RS11190 - hypothetical protein, at 711,352 to 712,698
_RS11190
Position (kb) 709
710
711 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 709.103 kb on + strand, within RPSI07_RS11175 at 709.103 kb on + strand, within RPSI07_RS11175 at 709.104 kb on - strand, within RPSI07_RS11175 at 709.135 kb on + strand, within RPSI07_RS11175 at 709.179 kb on - strand, within RPSI07_RS11175 at 709.232 kb on + strand, within RPSI07_RS11175 at 709.233 kb on - strand, within RPSI07_RS11175 at 709.257 kb on - strand, within RPSI07_RS11175 at 709.682 kb on + strand at 709.683 kb on - strand at 709.683 kb on - strand at 709.683 kb on - strand at 709.716 kb on - strand at 710.182 kb on + strand, within RPSI07_RS11180 at 710.183 kb on - strand, within RPSI07_RS11180 at 710.183 kb on - strand, within RPSI07_RS11180 at 710.184 kb on + strand, within RPSI07_RS11180 at 710.184 kb on + strand, within RPSI07_RS11180 at 710.184 kb on + strand, within RPSI07_RS11180 at 710.185 kb on - strand, within RPSI07_RS11180 at 710.185 kb on - strand, within RPSI07_RS11180 at 710.185 kb on - strand, within RPSI07_RS11180 at 710.217 kb on + strand, within RPSI07_RS11180 at 710.406 kb on + strand, within RPSI07_RS11180 at 710.407 kb on - strand, within RPSI07_RS11180 at 710.442 kb on + strand, within RPSI07_RS11180 at 710.443 kb on - strand, within RPSI07_RS11180 at 710.728 kb on + strand at 710.729 kb on - strand at 710.858 kb on - strand at 710.866 kb on - strand at 711.128 kb on + strand, within RPSI07_RS11185 at 711.129 kb on - strand, within RPSI07_RS11185 at 711.129 kb on - strand, within RPSI07_RS11185 at 711.189 kb on - strand, within RPSI07_RS11185 at 711.189 kb on - strand, within RPSI07_RS11185 at 711.272 kb on + strand at 711.273 kb on - strand at 711.680 kb on + strand, within RPSI07_RS11190 at 711.680 kb on + strand, within RPSI07_RS11190
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol_and_CrystalViolet remove 709,103 + RPSI07_RS11175 0.39 +1.1 709,103 + RPSI07_RS11175 0.39 +2.6 709,104 - RPSI07_RS11175 0.39 +1.6 709,135 + RPSI07_RS11175 0.41 +3.3 709,179 - RPSI07_RS11175 0.46 +2.6 709,232 + RPSI07_RS11175 0.50 -1.7 709,233 - RPSI07_RS11175 0.51 -2.4 709,257 - RPSI07_RS11175 0.53 +0.3 709,682 + +1.2 709,683 - +1.3 709,683 - +1.5 709,683 - +0.0 709,716 - -0.5 710,182 + RPSI07_RS11180 0.43 -1.1 710,183 - RPSI07_RS11180 0.43 -0.0 710,183 - RPSI07_RS11180 0.43 +1.4 710,184 + RPSI07_RS11180 0.43 +1.4 710,184 + RPSI07_RS11180 0.43 -0.4 710,184 + RPSI07_RS11180 0.43 +2.0 710,185 - RPSI07_RS11180 0.43 -0.0 710,185 - RPSI07_RS11180 0.43 -0.1 710,185 - RPSI07_RS11180 0.43 -0.5 710,217 + RPSI07_RS11180 0.47 -1.2 710,406 + RPSI07_RS11180 0.68 +1.5 710,407 - RPSI07_RS11180 0.68 -0.3 710,442 + RPSI07_RS11180 0.72 +0.3 710,443 - RPSI07_RS11180 0.72 -0.2 710,728 + +1.7 710,729 - -0.1 710,858 - -0.0 710,866 - -0.0 711,128 + RPSI07_RS11185 0.61 +3.0 711,129 - RPSI07_RS11185 0.61 -0.0 711,129 - RPSI07_RS11185 0.61 -0.5 711,189 - RPSI07_RS11185 0.74 +1.6 711,189 - RPSI07_RS11185 0.74 -1.8 711,272 + -0.4 711,273 - +0.1 711,680 + RPSI07_RS11190 0.24 +0.6 711,680 + RPSI07_RS11190 0.24 -0.3
Or see this region's nucleotide sequence