Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS23420 and RPSI07_RS23425 are separated by 13 nucleotides RPSI07_RS23425 and RPSI07_RS23430 overlap by 4 nucleotides RPSI07_RS23430 and RPSI07_RS23435 are separated by 9 nucleotides
RPSI07_RS23420: RPSI07_RS23420 - phosphomethylpyrimidine synthase ThiC, at 3,400,159 to 3,402,054
_RS23420
RPSI07_RS23425: RPSI07_RS23425 - FAD-dependent oxidoreductase, at 3,402,068 to 3,403,207
_RS23425
RPSI07_RS23430: RPSI07_RS23430 - thiamine biosynthesis protein ThiS, at 3,403,204 to 3,403,431
_RS23430
RPSI07_RS23435: RPSI07_RS23435 - thiazole synthase, at 3,403,441 to 3,404,268
_RS23435
Position (kb)
3402
3403
3404 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3401.176 kb on + strand, within RPSI07_RS23420 at 3401.176 kb on + strand, within RPSI07_RS23420 at 3401.176 kb on + strand, within RPSI07_RS23420 at 3401.275 kb on + strand, within RPSI07_RS23420 at 3401.275 kb on + strand, within RPSI07_RS23420 at 3401.275 kb on + strand, within RPSI07_RS23420 at 3401.314 kb on + strand, within RPSI07_RS23420 at 3401.441 kb on + strand, within RPSI07_RS23420 at 3401.441 kb on + strand, within RPSI07_RS23420 at 3401.441 kb on + strand, within RPSI07_RS23420 at 3401.441 kb on + strand, within RPSI07_RS23420 at 3401.441 kb on + strand, within RPSI07_RS23420 at 3401.575 kb on + strand, within RPSI07_RS23420 at 3401.620 kb on + strand, within RPSI07_RS23420 at 3401.641 kb on + strand, within RPSI07_RS23420 at 3401.707 kb on + strand, within RPSI07_RS23420 at 3401.707 kb on + strand, within RPSI07_RS23420 at 3401.707 kb on + strand, within RPSI07_RS23420 at 3401.956 kb on + strand at 3401.956 kb on + strand at 3401.993 kb on + strand at 3402.046 kb on + strand at 3402.046 kb on + strand at 3402.046 kb on + strand at 3402.046 kb on + strand at 3402.815 kb on + strand, within RPSI07_RS23425 at 3402.815 kb on + strand, within RPSI07_RS23425 at 3402.815 kb on + strand, within RPSI07_RS23425 at 3402.815 kb on + strand, within RPSI07_RS23425 at 3402.842 kb on + strand, within RPSI07_RS23425 at 3402.842 kb on + strand, within RPSI07_RS23425 at 3402.842 kb on + strand, within RPSI07_RS23425
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 3,401,176 + RPSI07_RS23420 0.54 -0.5 3,401,176 + RPSI07_RS23420 0.54 -1.0 3,401,176 + RPSI07_RS23420 0.54 -0.5 3,401,275 + RPSI07_RS23420 0.59 +1.6 3,401,275 + RPSI07_RS23420 0.59 -0.7 3,401,275 + RPSI07_RS23420 0.59 +0.7 3,401,314 + RPSI07_RS23420 0.61 +0.4 3,401,441 + RPSI07_RS23420 0.68 +0.0 3,401,441 + RPSI07_RS23420 0.68 +0.7 3,401,441 + RPSI07_RS23420 0.68 +0.1 3,401,441 + RPSI07_RS23420 0.68 +0.0 3,401,441 + RPSI07_RS23420 0.68 +1.0 3,401,575 + RPSI07_RS23420 0.75 +3.0 3,401,620 + RPSI07_RS23420 0.77 -0.5 3,401,641 + RPSI07_RS23420 0.78 +1.2 3,401,707 + RPSI07_RS23420 0.82 +1.0 3,401,707 + RPSI07_RS23420 0.82 -0.4 3,401,707 + RPSI07_RS23420 0.82 +1.3 3,401,956 + +1.0 3,401,956 + -0.7 3,401,993 + -0.8 3,402,046 + -1.4 3,402,046 + +1.0 3,402,046 + +1.4 3,402,046 + +0.9 3,402,815 + RPSI07_RS23425 0.66 +0.0 3,402,815 + RPSI07_RS23425 0.66 +0.0 3,402,815 + RPSI07_RS23425 0.66 -0.5 3,402,815 + RPSI07_RS23425 0.66 -1.0 3,402,842 + RPSI07_RS23425 0.68 -0.5 3,402,842 + RPSI07_RS23425 0.68 -2.1 3,402,842 + RPSI07_RS23425 0.68 -1.4
Or see this region's nucleotide sequence