Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS23425

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS23420 and RPSI07_RS23425 are separated by 13 nucleotidesRPSI07_RS23425 and RPSI07_RS23430 overlap by 4 nucleotidesRPSI07_RS23430 and RPSI07_RS23435 are separated by 9 nucleotides RPSI07_RS23420: RPSI07_RS23420 - phosphomethylpyrimidine synthase ThiC, at 3,400,159 to 3,402,054 _RS23420 RPSI07_RS23425: RPSI07_RS23425 - FAD-dependent oxidoreductase, at 3,402,068 to 3,403,207 _RS23425 RPSI07_RS23430: RPSI07_RS23430 - thiamine biosynthesis protein ThiS, at 3,403,204 to 3,403,431 _RS23430 RPSI07_RS23435: RPSI07_RS23435 - thiazole synthase, at 3,403,441 to 3,404,268 _RS23435 Position (kb) 3402 3403 3404Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3401.176 kb on + strand, within RPSI07_RS23420at 3401.176 kb on + strand, within RPSI07_RS23420at 3401.176 kb on + strand, within RPSI07_RS23420at 3401.275 kb on + strand, within RPSI07_RS23420at 3401.275 kb on + strand, within RPSI07_RS23420at 3401.275 kb on + strand, within RPSI07_RS23420at 3401.314 kb on + strand, within RPSI07_RS23420at 3401.441 kb on + strand, within RPSI07_RS23420at 3401.441 kb on + strand, within RPSI07_RS23420at 3401.441 kb on + strand, within RPSI07_RS23420at 3401.441 kb on + strand, within RPSI07_RS23420at 3401.441 kb on + strand, within RPSI07_RS23420at 3401.575 kb on + strand, within RPSI07_RS23420at 3401.620 kb on + strand, within RPSI07_RS23420at 3401.641 kb on + strand, within RPSI07_RS23420at 3401.707 kb on + strand, within RPSI07_RS23420at 3401.707 kb on + strand, within RPSI07_RS23420at 3401.707 kb on + strand, within RPSI07_RS23420at 3401.956 kb on + strandat 3401.956 kb on + strandat 3401.993 kb on + strandat 3402.046 kb on + strandat 3402.046 kb on + strandat 3402.046 kb on + strandat 3402.046 kb on + strandat 3402.815 kb on + strand, within RPSI07_RS23425at 3402.815 kb on + strand, within RPSI07_RS23425at 3402.815 kb on + strand, within RPSI07_RS23425at 3402.815 kb on + strand, within RPSI07_RS23425at 3402.842 kb on + strand, within RPSI07_RS23425at 3402.842 kb on + strand, within RPSI07_RS23425at 3402.842 kb on + strand, within RPSI07_RS23425

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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3,401,176 + RPSI07_RS23420 0.54 -0.5
3,401,176 + RPSI07_RS23420 0.54 -1.0
3,401,176 + RPSI07_RS23420 0.54 -0.5
3,401,275 + RPSI07_RS23420 0.59 +1.6
3,401,275 + RPSI07_RS23420 0.59 -0.7
3,401,275 + RPSI07_RS23420 0.59 +0.7
3,401,314 + RPSI07_RS23420 0.61 +0.4
3,401,441 + RPSI07_RS23420 0.68 +0.0
3,401,441 + RPSI07_RS23420 0.68 +0.7
3,401,441 + RPSI07_RS23420 0.68 +0.1
3,401,441 + RPSI07_RS23420 0.68 +0.0
3,401,441 + RPSI07_RS23420 0.68 +1.0
3,401,575 + RPSI07_RS23420 0.75 +3.0
3,401,620 + RPSI07_RS23420 0.77 -0.5
3,401,641 + RPSI07_RS23420 0.78 +1.2
3,401,707 + RPSI07_RS23420 0.82 +1.0
3,401,707 + RPSI07_RS23420 0.82 -0.4
3,401,707 + RPSI07_RS23420 0.82 +1.3
3,401,956 + +1.0
3,401,956 + -0.7
3,401,993 + -0.8
3,402,046 + -1.4
3,402,046 + +1.0
3,402,046 + +1.4
3,402,046 + +0.9
3,402,815 + RPSI07_RS23425 0.66 +0.0
3,402,815 + RPSI07_RS23425 0.66 +0.0
3,402,815 + RPSI07_RS23425 0.66 -0.5
3,402,815 + RPSI07_RS23425 0.66 -1.0
3,402,842 + RPSI07_RS23425 0.68 -0.5
3,402,842 + RPSI07_RS23425 0.68 -2.1
3,402,842 + RPSI07_RS23425 0.68 -1.4

Or see this region's nucleotide sequence