Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS22410 and RPSI07_RS22415 are separated by 135 nucleotides RPSI07_RS22415 and RPSI07_RS22420 are separated by 72 nucleotides RPSI07_RS22420 and RPSI07_RS22425 are separated by 67 nucleotides
RPSI07_RS22410: RPSI07_RS22410 - 2,3-diphosphoglycerate-dependent phosphoglycerate mutase, at 3,185,201 to 3,185,947
_RS22410
RPSI07_RS22415: RPSI07_RS22415 - S41 family peptidase, at 3,186,083 to 3,187,699
_RS22415
RPSI07_RS22420: RPSI07_RS22420 - molybdopterin-synthase adenylyltransferase MoeB, at 3,187,772 to 3,188,521
_RS22420
RPSI07_RS22425: RPSI07_RS22425 - bacterioferritin, at 3,188,589 to 3,189,068
_RS22425
Position (kb)
3186
3187
3188 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3185.119 kb on + strand at 3185.181 kb on + strand at 3185.206 kb on + strand at 3185.207 kb on - strand at 3185.207 kb on - strand at 3185.361 kb on - strand, within RPSI07_RS22410 at 3185.469 kb on - strand, within RPSI07_RS22410 at 3185.549 kb on + strand, within RPSI07_RS22410 at 3185.550 kb on - strand, within RPSI07_RS22410 at 3185.841 kb on - strand, within RPSI07_RS22410 at 3185.841 kb on - strand, within RPSI07_RS22410 at 3185.877 kb on - strand at 3185.879 kb on + strand at 3185.879 kb on + strand at 3185.880 kb on - strand at 3185.880 kb on - strand at 3185.880 kb on - strand at 3185.945 kb on + strand at 3185.946 kb on - strand at 3185.993 kb on + strand at 3185.993 kb on + strand at 3185.993 kb on + strand at 3185.994 kb on - strand at 3185.995 kb on + strand at 3185.996 kb on - strand at 3186.046 kb on + strand at 3186.046 kb on + strand at 3186.046 kb on + strand at 3186.248 kb on + strand, within RPSI07_RS22415 at 3186.367 kb on + strand, within RPSI07_RS22415 at 3186.908 kb on + strand, within RPSI07_RS22415 at 3186.908 kb on + strand, within RPSI07_RS22415 at 3186.930 kb on - strand, within RPSI07_RS22415 at 3187.017 kb on - strand, within RPSI07_RS22415 at 3187.017 kb on - strand, within RPSI07_RS22415 at 3187.166 kb on + strand, within RPSI07_RS22415 at 3187.169 kb on + strand, within RPSI07_RS22415 at 3188.623 kb on + strand at 3188.624 kb on - strand at 3188.624 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 3,185,119 + +0.2 3,185,181 + -0.4 3,185,206 + -1.3 3,185,207 - -0.9 3,185,207 - -0.6 3,185,361 - RPSI07_RS22410 0.21 -0.0 3,185,469 - RPSI07_RS22410 0.36 -0.3 3,185,549 + RPSI07_RS22410 0.47 +1.0 3,185,550 - RPSI07_RS22410 0.47 +2.0 3,185,841 - RPSI07_RS22410 0.86 -1.6 3,185,841 - RPSI07_RS22410 0.86 -1.6 3,185,877 - +1.0 3,185,879 + -0.3 3,185,879 + +1.3 3,185,880 - -0.0 3,185,880 - +3.0 3,185,880 - +2.6 3,185,945 + +0.1 3,185,946 - +1.0 3,185,993 + +0.5 3,185,993 + -0.7 3,185,993 + +0.6 3,185,994 - -0.9 3,185,995 + -1.3 3,185,996 - -1.0 3,186,046 + -1.1 3,186,046 + -2.2 3,186,046 + +1.7 3,186,248 + RPSI07_RS22415 0.10 +1.0 3,186,367 + RPSI07_RS22415 0.18 -1.8 3,186,908 + RPSI07_RS22415 0.51 -1.0 3,186,908 + RPSI07_RS22415 0.51 -1.3 3,186,930 - RPSI07_RS22415 0.52 +0.0 3,187,017 - RPSI07_RS22415 0.58 +0.0 3,187,017 - RPSI07_RS22415 0.58 -0.6 3,187,166 + RPSI07_RS22415 0.67 -0.6 3,187,169 + RPSI07_RS22415 0.67 -2.1 3,188,623 + -1.0 3,188,624 - -1.1 3,188,624 - +1.0
Or see this region's nucleotide sequence