Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS18135

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS18130 and RPSI07_RS18135 are separated by 154 nucleotidesRPSI07_RS18135 and RPSI07_RS18140 are separated by 28 nucleotides RPSI07_RS18130: RPSI07_RS18130 - 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD, at 2,272,929 to 2,274,281 _RS18130 RPSI07_RS18135: RPSI07_RS18135 - endonuclease, at 2,274,436 to 2,275,305 _RS18135 RPSI07_RS18140: RPSI07_RS18140 - RNA polymerase sigma factor RpoS, at 2,275,334 to 2,276,467 _RS18140 Position (kb) 2274 2275 2276Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2273.549 kb on - strand, within RPSI07_RS18130at 2273.557 kb on + strand, within RPSI07_RS18130at 2273.558 kb on - strand, within RPSI07_RS18130at 2273.815 kb on + strand, within RPSI07_RS18130at 2273.815 kb on + strand, within RPSI07_RS18130at 2273.816 kb on - strand, within RPSI07_RS18130at 2273.816 kb on - strand, within RPSI07_RS18130at 2273.816 kb on - strand, within RPSI07_RS18130at 2274.417 kb on - strandat 2274.417 kb on - strandat 2274.417 kb on - strandat 2274.545 kb on + strand, within RPSI07_RS18135at 2274.546 kb on - strand, within RPSI07_RS18135at 2274.546 kb on - strandat 2274.546 kb on - strand, within RPSI07_RS18135at 2274.546 kb on - strand, within RPSI07_RS18135at 2274.859 kb on + strand, within RPSI07_RS18135at 2274.859 kb on + strand, within RPSI07_RS18135at 2275.093 kb on + strand, within RPSI07_RS18135at 2275.180 kb on + strand, within RPSI07_RS18135at 2275.180 kb on + strand, within RPSI07_RS18135at 2275.303 kb on + strandat 2275.335 kb on + strandat 2275.335 kb on + strandat 2275.506 kb on + strand, within RPSI07_RS18140at 2275.868 kb on - strand, within RPSI07_RS18140at 2275.894 kb on - strand, within RPSI07_RS18140at 2275.894 kb on - strand, within RPSI07_RS18140at 2275.894 kb on - strand, within RPSI07_RS18140at 2276.001 kb on + strand, within RPSI07_RS18140at 2276.145 kb on + strand, within RPSI07_RS18140

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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2,273,549 - RPSI07_RS18130 0.46 +1.5
2,273,557 + RPSI07_RS18130 0.46 -1.8
2,273,558 - RPSI07_RS18130 0.46 +0.4
2,273,815 + RPSI07_RS18130 0.65 -1.1
2,273,815 + RPSI07_RS18130 0.65 -1.3
2,273,816 - RPSI07_RS18130 0.66 -2.8
2,273,816 - RPSI07_RS18130 0.66 +0.9
2,273,816 - RPSI07_RS18130 0.66 -0.1
2,274,417 - -0.2
2,274,417 - +3.5
2,274,417 - -1.9
2,274,545 + RPSI07_RS18135 0.13 +1.5
2,274,546 - RPSI07_RS18135 0.13 -0.3
2,274,546 - +2.5
2,274,546 - RPSI07_RS18135 0.13 +1.5
2,274,546 - RPSI07_RS18135 0.13 -1.1
2,274,859 + RPSI07_RS18135 0.49 -0.1
2,274,859 + RPSI07_RS18135 0.49 -0.7
2,275,093 + RPSI07_RS18135 0.76 -0.7
2,275,180 + RPSI07_RS18135 0.86 +0.2
2,275,180 + RPSI07_RS18135 0.86 -0.5
2,275,303 + -2.4
2,275,335 + +0.9
2,275,335 + +0.1
2,275,506 + RPSI07_RS18140 0.15 +2.4
2,275,868 - RPSI07_RS18140 0.47 +1.8
2,275,894 - RPSI07_RS18140 0.49 +2.1
2,275,894 - RPSI07_RS18140 0.49 +2.4
2,275,894 - RPSI07_RS18140 0.49 +2.1
2,276,001 + RPSI07_RS18140 0.59 +2.0
2,276,145 + RPSI07_RS18140 0.72 +1.7

Or see this region's nucleotide sequence