Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS18130 and RPSI07_RS18135 are separated by 154 nucleotides RPSI07_RS18135 and RPSI07_RS18140 are separated by 28 nucleotides
RPSI07_RS18130: RPSI07_RS18130 - 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD, at 2,272,929 to 2,274,281
_RS18130
RPSI07_RS18135: RPSI07_RS18135 - endonuclease, at 2,274,436 to 2,275,305
_RS18135
RPSI07_RS18140: RPSI07_RS18140 - RNA polymerase sigma factor RpoS, at 2,275,334 to 2,276,467
_RS18140
Position (kb)
2274
2275
2276 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2273.549 kb on - strand, within RPSI07_RS18130 at 2273.557 kb on + strand, within RPSI07_RS18130 at 2273.558 kb on - strand, within RPSI07_RS18130 at 2273.815 kb on + strand, within RPSI07_RS18130 at 2273.815 kb on + strand, within RPSI07_RS18130 at 2273.816 kb on - strand, within RPSI07_RS18130 at 2273.816 kb on - strand, within RPSI07_RS18130 at 2273.816 kb on - strand, within RPSI07_RS18130 at 2274.417 kb on - strand at 2274.417 kb on - strand at 2274.417 kb on - strand at 2274.545 kb on + strand, within RPSI07_RS18135 at 2274.546 kb on - strand, within RPSI07_RS18135 at 2274.546 kb on - strand at 2274.546 kb on - strand, within RPSI07_RS18135 at 2274.546 kb on - strand, within RPSI07_RS18135 at 2274.859 kb on + strand, within RPSI07_RS18135 at 2274.859 kb on + strand, within RPSI07_RS18135 at 2275.093 kb on + strand, within RPSI07_RS18135 at 2275.180 kb on + strand, within RPSI07_RS18135 at 2275.180 kb on + strand, within RPSI07_RS18135 at 2275.303 kb on + strand at 2275.335 kb on + strand at 2275.335 kb on + strand at 2275.506 kb on + strand, within RPSI07_RS18140 at 2275.868 kb on - strand, within RPSI07_RS18140 at 2275.894 kb on - strand, within RPSI07_RS18140 at 2275.894 kb on - strand, within RPSI07_RS18140 at 2275.894 kb on - strand, within RPSI07_RS18140 at 2276.001 kb on + strand, within RPSI07_RS18140 at 2276.145 kb on + strand, within RPSI07_RS18140
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 2,273,549 - RPSI07_RS18130 0.46 +1.5 2,273,557 + RPSI07_RS18130 0.46 -1.8 2,273,558 - RPSI07_RS18130 0.46 +0.4 2,273,815 + RPSI07_RS18130 0.65 -1.1 2,273,815 + RPSI07_RS18130 0.65 -1.3 2,273,816 - RPSI07_RS18130 0.66 -2.8 2,273,816 - RPSI07_RS18130 0.66 +0.9 2,273,816 - RPSI07_RS18130 0.66 -0.1 2,274,417 - -0.2 2,274,417 - +3.5 2,274,417 - -1.9 2,274,545 + RPSI07_RS18135 0.13 +1.5 2,274,546 - RPSI07_RS18135 0.13 -0.3 2,274,546 - +2.5 2,274,546 - RPSI07_RS18135 0.13 +1.5 2,274,546 - RPSI07_RS18135 0.13 -1.1 2,274,859 + RPSI07_RS18135 0.49 -0.1 2,274,859 + RPSI07_RS18135 0.49 -0.7 2,275,093 + RPSI07_RS18135 0.76 -0.7 2,275,180 + RPSI07_RS18135 0.86 +0.2 2,275,180 + RPSI07_RS18135 0.86 -0.5 2,275,303 + -2.4 2,275,335 + +0.9 2,275,335 + +0.1 2,275,506 + RPSI07_RS18140 0.15 +2.4 2,275,868 - RPSI07_RS18140 0.47 +1.8 2,275,894 - RPSI07_RS18140 0.49 +2.1 2,275,894 - RPSI07_RS18140 0.49 +2.4 2,275,894 - RPSI07_RS18140 0.49 +2.1 2,276,001 + RPSI07_RS18140 0.59 +2.0 2,276,145 + RPSI07_RS18140 0.72 +1.7
Or see this region's nucleotide sequence