Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS18120

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS18115 and RPSI07_RS18120 are separated by 43 nucleotidesRPSI07_RS18120 and RPSI07_RS18125 are separated by 368 nucleotides RPSI07_RS18115: RPSI07_RS18115 - 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN, at 2,270,146 to 2,271,297 _RS18115 RPSI07_RS18120: RPSI07_RS18120 - nucleoside-diphosphate kinase, at 2,271,341 to 2,271,766 _RS18120 RPSI07_RS18125: RPSI07_RS18125 - BAX inhibitor (BI)-1/YccA family protein, at 2,272,135 to 2,272,836 _RS18125 Position (kb) 2271 2272Strain fitness (log2 ratio) -3 -2 -1 0 1at 2271.012 kb on - strand, within RPSI07_RS18115at 2271.012 kb on - strand, within RPSI07_RS18115at 2271.077 kb on - strand, within RPSI07_RS18115at 2271.330 kb on - strandat 2271.352 kb on - strandat 2272.219 kb on - strand, within RPSI07_RS18125at 2272.222 kb on + strand, within RPSI07_RS18125at 2272.357 kb on + strand, within RPSI07_RS18125at 2272.693 kb on + strand, within RPSI07_RS18125at 2272.703 kb on - strand, within RPSI07_RS18125at 2272.765 kb on + strand, within RPSI07_RS18125at 2272.766 kb on - strandat 2272.766 kb on - strandat 2272.766 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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2,271,012 - RPSI07_RS18115 0.75 -1.4
2,271,012 - RPSI07_RS18115 0.75 +0.9
2,271,077 - RPSI07_RS18115 0.81 -3.1
2,271,330 - -0.8
2,271,352 - +0.2
2,272,219 - RPSI07_RS18125 0.12 +0.2
2,272,222 + RPSI07_RS18125 0.12 +0.9
2,272,357 + RPSI07_RS18125 0.32 +0.2
2,272,693 + RPSI07_RS18125 0.79 -0.8
2,272,703 - RPSI07_RS18125 0.81 -1.1
2,272,765 + RPSI07_RS18125 0.90 +0.9
2,272,766 - +0.4
2,272,766 - -2.4
2,272,766 - -0.3

Or see this region's nucleotide sequence