Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS17600 and RPSI07_RS17605 are separated by 249 nucleotides RPSI07_RS17605 and RPSI07_RS25150 are separated by 115 nucleotides RPSI07_RS25150 and RPSI07_RS25155 are separated by 68 nucleotides RPSI07_RS25155 and RPSI07_RS25370 are separated by 143 nucleotides RPSI07_RS25370 and RPSI07_RS17615 are separated by 16 nucleotides
RPSI07_RS17600: RPSI07_RS17600 - ferredoxin--NADP reductase, at 2,151,642 to 2,152,418
_RS17600
RPSI07_RS17605: RPSI07_RS17605 - transcriptional repressor LexA, at 2,152,668 to 2,153,318
_RS17605
RPSI07_RS25150: RPSI07_RS25150 - hypothetical protein, at 2,153,434 to 2,153,619
_RS25150
RPSI07_RS25155: RPSI07_RS25155 - hypothetical protein, at 2,153,688 to 2,153,891
_RS25155
RPSI07_RS25370: RPSI07_RS25370 - disulfide bond formation protein DsbH, at 2,154,035 to 2,154,209
_RS25370
RPSI07_RS17615: RPSI07_RS17615 - aminoacyl-histidine dipeptidase, at 2,154,226 to 2,155,959
_RS17615
Position (kb)
2152
2153
2154 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2152.515 kb on + strand at 2152.516 kb on - strand at 2152.516 kb on - strand at 2152.516 kb on - strand at 2153.513 kb on - strand, within RPSI07_RS25150 at 2153.513 kb on - strand at 2153.513 kb on - strand, within RPSI07_RS25150 at 2153.837 kb on + strand, within RPSI07_RS25155 at 2153.838 kb on - strand, within RPSI07_RS25155 at 2153.838 kb on - strand, within RPSI07_RS25155 at 2153.838 kb on - strand, within RPSI07_RS25155 at 2153.838 kb on - strand, within RPSI07_RS25155 at 2153.840 kb on + strand, within RPSI07_RS25155 at 2153.841 kb on - strand, within RPSI07_RS25155 at 2153.923 kb on + strand at 2153.924 kb on - strand at 2154.052 kb on - strand at 2154.143 kb on + strand, within RPSI07_RS25370 at 2154.153 kb on - strand, within RPSI07_RS25370 at 2154.171 kb on - strand, within RPSI07_RS25370 at 2154.171 kb on - strand, within RPSI07_RS25370 at 2154.301 kb on + strand at 2154.311 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 2,152,515 + -0.1 2,152,516 - +1.6 2,152,516 - -0.3 2,152,516 - +0.5 2,153,513 - RPSI07_RS25150 0.42 -0.3 2,153,513 - -2.1 2,153,513 - RPSI07_RS25150 0.42 +3.1 2,153,837 + RPSI07_RS25155 0.73 -0.6 2,153,838 - RPSI07_RS25155 0.74 -0.5 2,153,838 - RPSI07_RS25155 0.74 +0.1 2,153,838 - RPSI07_RS25155 0.74 +0.8 2,153,838 - RPSI07_RS25155 0.74 -0.2 2,153,840 + RPSI07_RS25155 0.75 -0.4 2,153,841 - RPSI07_RS25155 0.75 +1.5 2,153,923 + -1.4 2,153,924 - -1.5 2,154,052 - +1.3 2,154,143 + RPSI07_RS25370 0.62 +0.7 2,154,153 - RPSI07_RS25370 0.67 +2.9 2,154,171 - RPSI07_RS25370 0.78 +0.1 2,154,171 - RPSI07_RS25370 0.78 -1.5 2,154,301 + +0.9 2,154,311 + -1.0
Or see this region's nucleotide sequence