Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS17250 and RPSI07_RS17255 are separated by 238 nucleotides RPSI07_RS17255 and RPSI07_RS17260 are separated by 31 nucleotides
RPSI07_RS17250: RPSI07_RS17250 - MurR/RpiR family transcriptional regulator, at 2,077,055 to 2,078,053
_RS17250
RPSI07_RS17255: RPSI07_RS17255 - ABC transporter, at 2,078,292 to 2,080,238
_RS17255
RPSI07_RS17260: RPSI07_RS17260 - CoA transferase, at 2,080,270 to 2,081,376
_RS17260
Position (kb)
2078
2079
2080
2081 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2077.305 kb on + strand, within RPSI07_RS17250 at 2077.306 kb on - strand, within RPSI07_RS17250 at 2077.530 kb on + strand, within RPSI07_RS17250 at 2077.530 kb on + strand, within RPSI07_RS17250 at 2077.530 kb on + strand, within RPSI07_RS17250 at 2077.567 kb on - strand, within RPSI07_RS17250 at 2077.593 kb on + strand, within RPSI07_RS17250 at 2077.593 kb on + strand, within RPSI07_RS17250 at 2077.593 kb on + strand, within RPSI07_RS17250 at 2077.593 kb on + strand, within RPSI07_RS17250 at 2077.593 kb on + strand, within RPSI07_RS17250 at 2077.594 kb on - strand, within RPSI07_RS17250 at 2077.926 kb on + strand, within RPSI07_RS17250 at 2077.926 kb on + strand, within RPSI07_RS17250 at 2077.926 kb on + strand, within RPSI07_RS17250 at 2077.927 kb on - strand, within RPSI07_RS17250 at 2078.080 kb on - strand at 2078.086 kb on - strand at 2078.090 kb on - strand at 2078.107 kb on - strand at 2078.107 kb on - strand at 2078.132 kb on + strand at 2078.132 kb on + strand at 2078.134 kb on - strand at 2078.144 kb on + strand at 2078.250 kb on + strand at 2078.251 kb on - strand at 2078.251 kb on - strand at 2078.538 kb on + strand, within RPSI07_RS17255 at 2078.538 kb on + strand, within RPSI07_RS17255 at 2078.539 kb on - strand, within RPSI07_RS17255 at 2078.539 kb on - strand, within RPSI07_RS17255 at 2078.659 kb on - strand, within RPSI07_RS17255 at 2078.659 kb on - strand, within RPSI07_RS17255 at 2078.982 kb on + strand, within RPSI07_RS17255 at 2078.983 kb on - strand, within RPSI07_RS17255 at 2078.983 kb on - strand, within RPSI07_RS17255 at 2079.444 kb on + strand, within RPSI07_RS17255 at 2079.445 kb on - strand, within RPSI07_RS17255 at 2080.735 kb on + strand, within RPSI07_RS17260 at 2080.736 kb on - strand, within RPSI07_RS17260 at 2080.736 kb on - strand, within RPSI07_RS17260 at 2080.736 kb on - strand, within RPSI07_RS17260
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 2,077,305 + RPSI07_RS17250 0.25 +3.5 2,077,306 - RPSI07_RS17250 0.25 +0.7 2,077,530 + RPSI07_RS17250 0.48 -1.6 2,077,530 + RPSI07_RS17250 0.48 -2.5 2,077,530 + RPSI07_RS17250 0.48 -0.0 2,077,567 - RPSI07_RS17250 0.51 -1.4 2,077,593 + RPSI07_RS17250 0.54 -1.2 2,077,593 + RPSI07_RS17250 0.54 -0.8 2,077,593 + RPSI07_RS17250 0.54 -1.1 2,077,593 + RPSI07_RS17250 0.54 +0.1 2,077,593 + RPSI07_RS17250 0.54 +1.2 2,077,594 - RPSI07_RS17250 0.54 +1.2 2,077,926 + RPSI07_RS17250 0.87 +1.4 2,077,926 + RPSI07_RS17250 0.87 -2.7 2,077,926 + RPSI07_RS17250 0.87 -1.8 2,077,927 - RPSI07_RS17250 0.87 +0.9 2,078,080 - -0.0 2,078,086 - +0.2 2,078,090 - -0.5 2,078,107 - -0.7 2,078,107 - -0.3 2,078,132 + -0.5 2,078,132 + -0.2 2,078,134 - +1.1 2,078,144 + +0.3 2,078,250 + +0.1 2,078,251 - +1.0 2,078,251 - +0.1 2,078,538 + RPSI07_RS17255 0.13 -1.7 2,078,538 + RPSI07_RS17255 0.13 -1.6 2,078,539 - RPSI07_RS17255 0.13 -1.0 2,078,539 - RPSI07_RS17255 0.13 +0.1 2,078,659 - RPSI07_RS17255 0.19 -1.3 2,078,659 - RPSI07_RS17255 0.19 -2.3 2,078,982 + RPSI07_RS17255 0.35 +0.2 2,078,983 - RPSI07_RS17255 0.35 +1.3 2,078,983 - RPSI07_RS17255 0.35 +0.3 2,079,444 + RPSI07_RS17255 0.59 +0.1 2,079,445 - RPSI07_RS17255 0.59 -1.1 2,080,735 + RPSI07_RS17260 0.42 -0.2 2,080,736 - RPSI07_RS17260 0.42 +3.7 2,080,736 - RPSI07_RS17260 0.42 -0.9 2,080,736 - RPSI07_RS17260 0.42 -0.9
Or see this region's nucleotide sequence