Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS17255

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS17250 and RPSI07_RS17255 are separated by 238 nucleotidesRPSI07_RS17255 and RPSI07_RS17260 are separated by 31 nucleotides RPSI07_RS17250: RPSI07_RS17250 - MurR/RpiR family transcriptional regulator, at 2,077,055 to 2,078,053 _RS17250 RPSI07_RS17255: RPSI07_RS17255 - ABC transporter, at 2,078,292 to 2,080,238 _RS17255 RPSI07_RS17260: RPSI07_RS17260 - CoA transferase, at 2,080,270 to 2,081,376 _RS17260 Position (kb) 2078 2079 2080 2081Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2077.305 kb on + strand, within RPSI07_RS17250at 2077.306 kb on - strand, within RPSI07_RS17250at 2077.530 kb on + strand, within RPSI07_RS17250at 2077.530 kb on + strand, within RPSI07_RS17250at 2077.530 kb on + strand, within RPSI07_RS17250at 2077.567 kb on - strand, within RPSI07_RS17250at 2077.593 kb on + strand, within RPSI07_RS17250at 2077.593 kb on + strand, within RPSI07_RS17250at 2077.593 kb on + strand, within RPSI07_RS17250at 2077.593 kb on + strand, within RPSI07_RS17250at 2077.593 kb on + strand, within RPSI07_RS17250at 2077.594 kb on - strand, within RPSI07_RS17250at 2077.926 kb on + strand, within RPSI07_RS17250at 2077.926 kb on + strand, within RPSI07_RS17250at 2077.926 kb on + strand, within RPSI07_RS17250at 2077.927 kb on - strand, within RPSI07_RS17250at 2078.080 kb on - strandat 2078.086 kb on - strandat 2078.090 kb on - strandat 2078.107 kb on - strandat 2078.107 kb on - strandat 2078.132 kb on + strandat 2078.132 kb on + strandat 2078.134 kb on - strandat 2078.144 kb on + strandat 2078.250 kb on + strandat 2078.251 kb on - strandat 2078.251 kb on - strandat 2078.538 kb on + strand, within RPSI07_RS17255at 2078.538 kb on + strand, within RPSI07_RS17255at 2078.539 kb on - strand, within RPSI07_RS17255at 2078.539 kb on - strand, within RPSI07_RS17255at 2078.659 kb on - strand, within RPSI07_RS17255at 2078.659 kb on - strand, within RPSI07_RS17255at 2078.982 kb on + strand, within RPSI07_RS17255at 2078.983 kb on - strand, within RPSI07_RS17255at 2078.983 kb on - strand, within RPSI07_RS17255at 2079.444 kb on + strand, within RPSI07_RS17255at 2079.445 kb on - strand, within RPSI07_RS17255at 2080.735 kb on + strand, within RPSI07_RS17260at 2080.736 kb on - strand, within RPSI07_RS17260at 2080.736 kb on - strand, within RPSI07_RS17260at 2080.736 kb on - strand, within RPSI07_RS17260

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
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2,077,305 + RPSI07_RS17250 0.25 +3.5
2,077,306 - RPSI07_RS17250 0.25 +0.7
2,077,530 + RPSI07_RS17250 0.48 -1.6
2,077,530 + RPSI07_RS17250 0.48 -2.5
2,077,530 + RPSI07_RS17250 0.48 -0.0
2,077,567 - RPSI07_RS17250 0.51 -1.4
2,077,593 + RPSI07_RS17250 0.54 -1.2
2,077,593 + RPSI07_RS17250 0.54 -0.8
2,077,593 + RPSI07_RS17250 0.54 -1.1
2,077,593 + RPSI07_RS17250 0.54 +0.1
2,077,593 + RPSI07_RS17250 0.54 +1.2
2,077,594 - RPSI07_RS17250 0.54 +1.2
2,077,926 + RPSI07_RS17250 0.87 +1.4
2,077,926 + RPSI07_RS17250 0.87 -2.7
2,077,926 + RPSI07_RS17250 0.87 -1.8
2,077,927 - RPSI07_RS17250 0.87 +0.9
2,078,080 - -0.0
2,078,086 - +0.2
2,078,090 - -0.5
2,078,107 - -0.7
2,078,107 - -0.3
2,078,132 + -0.5
2,078,132 + -0.2
2,078,134 - +1.1
2,078,144 + +0.3
2,078,250 + +0.1
2,078,251 - +1.0
2,078,251 - +0.1
2,078,538 + RPSI07_RS17255 0.13 -1.7
2,078,538 + RPSI07_RS17255 0.13 -1.6
2,078,539 - RPSI07_RS17255 0.13 -1.0
2,078,539 - RPSI07_RS17255 0.13 +0.1
2,078,659 - RPSI07_RS17255 0.19 -1.3
2,078,659 - RPSI07_RS17255 0.19 -2.3
2,078,982 + RPSI07_RS17255 0.35 +0.2
2,078,983 - RPSI07_RS17255 0.35 +1.3
2,078,983 - RPSI07_RS17255 0.35 +0.3
2,079,444 + RPSI07_RS17255 0.59 +0.1
2,079,445 - RPSI07_RS17255 0.59 -1.1
2,080,735 + RPSI07_RS17260 0.42 -0.2
2,080,736 - RPSI07_RS17260 0.42 +3.7
2,080,736 - RPSI07_RS17260 0.42 -0.9
2,080,736 - RPSI07_RS17260 0.42 -0.9

Or see this region's nucleotide sequence