Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS15685 and RPSI07_RS15690 are separated by 22 nucleotides RPSI07_RS15690 and RPSI07_RS15695 are separated by 364 nucleotides RPSI07_RS15695 and RPSI07_RS15700 are separated by 28 nucleotides RPSI07_RS15700 and RPSI07_RS15705 are separated by 196 nucleotides RPSI07_RS15705 and RPSI07_RS15710 are separated by 26 nucleotides
RPSI07_RS15685: RPSI07_RS15685 - bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase, at 1,677,487 to 1,679,727
_RS15685
RPSI07_RS15690: RPSI07_RS15690 - tRNA-Val, at 1,679,750 to 1,679,826
_RS15690
RPSI07_RS15695: RPSI07_RS15695 - threonine--tRNA ligase, at 1,680,191 to 1,682,098
_RS15695
RPSI07_RS15700: RPSI07_RS15700 - translation initiation factor IF-3, at 1,682,127 to 1,682,663
_RS15700
RPSI07_RS15705: RPSI07_RS15705 - 50S ribosomal protein L35, at 1,682,860 to 1,683,057
_RS15705
RPSI07_RS15710: RPSI07_RS15710 - 50S ribosomal protein L20, at 1,683,084 to 1,683,440
_RS15710
Position (kb) 1680
1681
1682
1683 Strain fitness (log2 ratio) -1
0
1
2 at 1679.501 kb on - strand, within RPSI07_RS15685 at 1679.685 kb on - strand at 1679.888 kb on + strand at 1679.898 kb on + strand at 1679.930 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 1,679,501 - RPSI07_RS15685 0.90 +1.4 1,679,685 - +0.6 1,679,888 + +1.4 1,679,898 + -0.6 1,679,930 + +2.4
Or see this region's nucleotide sequence