Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS14145 and RPSI07_RS14150 are separated by 234 nucleotides RPSI07_RS14150 and RPSI07_RS14155 are separated by 17 nucleotides
RPSI07_RS14145: RPSI07_RS14145 - UPF0016 domain-containing protein, at 1,361,528 to 1,362,100
_RS14145
RPSI07_RS14150: RPSI07_RS14150 - guanine deaminase, at 1,362,335 to 1,363,672
_RS14150
RPSI07_RS14155: RPSI07_RS14155 - adenosine deaminase, at 1,363,690 to 1,364,727
_RS14155
Position (kb)
1362
1363
1364 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1361.529 kb on + strand at 1361.529 kb on + strand at 1361.529 kb on + strand at 1361.530 kb on - strand at 1361.530 kb on - strand at 1362.302 kb on + strand at 1362.325 kb on + strand at 1362.326 kb on - strand at 1362.381 kb on + strand at 1362.381 kb on + strand at 1362.381 kb on + strand at 1362.381 kb on + strand at 1362.381 kb on + strand at 1362.382 kb on - strand at 1362.382 kb on - strand at 1362.382 kb on - strand at 1362.382 kb on - strand at 1362.382 kb on - strand at 1362.382 kb on - strand at 1362.585 kb on + strand, within RPSI07_RS14150 at 1362.585 kb on + strand, within RPSI07_RS14150 at 1362.586 kb on - strand, within RPSI07_RS14150 at 1362.586 kb on - strand, within RPSI07_RS14150 at 1362.738 kb on + strand, within RPSI07_RS14150 at 1362.738 kb on + strand, within RPSI07_RS14150 at 1362.738 kb on + strand, within RPSI07_RS14150 at 1362.739 kb on - strand, within RPSI07_RS14150 at 1362.835 kb on + strand, within RPSI07_RS14150 at 1362.835 kb on + strand, within RPSI07_RS14150 at 1362.836 kb on - strand, within RPSI07_RS14150 at 1362.836 kb on - strand, within RPSI07_RS14150 at 1362.836 kb on - strand, within RPSI07_RS14150 at 1362.836 kb on - strand, within RPSI07_RS14150 at 1362.847 kb on - strand, within RPSI07_RS14150 at 1362.883 kb on - strand, within RPSI07_RS14150 at 1362.894 kb on + strand, within RPSI07_RS14150 at 1362.894 kb on + strand, within RPSI07_RS14150 at 1362.894 kb on + strand, within RPSI07_RS14150 at 1362.894 kb on + strand, within RPSI07_RS14150 at 1362.894 kb on + strand, within RPSI07_RS14150 at 1362.981 kb on + strand, within RPSI07_RS14150 at 1363.138 kb on - strand, within RPSI07_RS14150 at 1363.315 kb on - strand, within RPSI07_RS14150 at 1363.353 kb on + strand, within RPSI07_RS14150 at 1363.353 kb on + strand, within RPSI07_RS14150 at 1363.353 kb on + strand, within RPSI07_RS14150 at 1363.353 kb on + strand, within RPSI07_RS14150 at 1363.354 kb on - strand, within RPSI07_RS14150 at 1363.354 kb on - strand, within RPSI07_RS14150 at 1363.461 kb on + strand, within RPSI07_RS14150 at 1363.461 kb on + strand, within RPSI07_RS14150 at 1363.461 kb on + strand, within RPSI07_RS14150 at 1363.461 kb on + strand, within RPSI07_RS14150 at 1363.462 kb on - strand, within RPSI07_RS14150 at 1363.510 kb on - strand, within RPSI07_RS14150 at 1363.510 kb on - strand, within RPSI07_RS14150 at 1363.566 kb on + strand at 1363.567 kb on - strand at 1363.590 kb on + strand at 1363.590 kb on + strand at 1363.630 kb on - strand at 1363.850 kb on + strand, within RPSI07_RS14155 at 1363.850 kb on + strand, within RPSI07_RS14155 at 1363.851 kb on - strand, within RPSI07_RS14155 at 1363.863 kb on - strand, within RPSI07_RS14155 at 1363.944 kb on - strand, within RPSI07_RS14155 at 1364.090 kb on + strand, within RPSI07_RS14155 at 1364.090 kb on + strand, within RPSI07_RS14155 at 1364.243 kb on + strand at 1364.243 kb on + strand, within RPSI07_RS14155 at 1364.389 kb on + strand, within RPSI07_RS14155 at 1364.389 kb on + strand, within RPSI07_RS14155 at 1364.390 kb on - strand, within RPSI07_RS14155 at 1364.390 kb on - strand, within RPSI07_RS14155 at 1364.468 kb on + strand, within RPSI07_RS14155 at 1364.468 kb on + strand, within RPSI07_RS14155 at 1364.469 kb on - strand, within RPSI07_RS14155 at 1364.525 kb on + strand, within RPSI07_RS14155 at 1364.528 kb on + strand, within RPSI07_RS14155 at 1364.528 kb on + strand, within RPSI07_RS14155 at 1364.529 kb on - strand, within RPSI07_RS14155
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 1,361,529 + -2.7 1,361,529 + -1.4 1,361,529 + -0.2 1,361,530 - +0.5 1,361,530 - +1.2 1,362,302 + -0.1 1,362,325 + -0.1 1,362,326 - -0.2 1,362,381 + -2.2 1,362,381 + -0.5 1,362,381 + +0.2 1,362,381 + +2.2 1,362,381 + -0.8 1,362,382 - +3.4 1,362,382 - +0.4 1,362,382 - -0.2 1,362,382 - +1.5 1,362,382 - -1.0 1,362,382 - +0.2 1,362,585 + RPSI07_RS14150 0.19 -0.7 1,362,585 + RPSI07_RS14150 0.19 +0.1 1,362,586 - RPSI07_RS14150 0.19 -2.3 1,362,586 - RPSI07_RS14150 0.19 -2.0 1,362,738 + RPSI07_RS14150 0.30 +1.1 1,362,738 + RPSI07_RS14150 0.30 +2.5 1,362,738 + RPSI07_RS14150 0.30 +0.9 1,362,739 - RPSI07_RS14150 0.30 -1.4 1,362,835 + RPSI07_RS14150 0.37 +0.9 1,362,835 + RPSI07_RS14150 0.37 -1.5 1,362,836 - RPSI07_RS14150 0.37 -0.0 1,362,836 - RPSI07_RS14150 0.37 -1.8 1,362,836 - RPSI07_RS14150 0.37 -0.9 1,362,836 - RPSI07_RS14150 0.37 -0.3 1,362,847 - RPSI07_RS14150 0.38 -1.1 1,362,883 - RPSI07_RS14150 0.41 +0.6 1,362,894 + RPSI07_RS14150 0.42 +0.9 1,362,894 + RPSI07_RS14150 0.42 -1.4 1,362,894 + RPSI07_RS14150 0.42 -2.6 1,362,894 + RPSI07_RS14150 0.42 -1.6 1,362,894 + RPSI07_RS14150 0.42 -0.1 1,362,981 + RPSI07_RS14150 0.48 -0.1 1,363,138 - RPSI07_RS14150 0.60 +2.5 1,363,315 - RPSI07_RS14150 0.73 +0.3 1,363,353 + RPSI07_RS14150 0.76 +0.2 1,363,353 + RPSI07_RS14150 0.76 -0.8 1,363,353 + RPSI07_RS14150 0.76 +0.1 1,363,353 + RPSI07_RS14150 0.76 -1.8 1,363,354 - RPSI07_RS14150 0.76 +0.3 1,363,354 - RPSI07_RS14150 0.76 -0.2 1,363,461 + RPSI07_RS14150 0.84 +2.2 1,363,461 + RPSI07_RS14150 0.84 -1.1 1,363,461 + RPSI07_RS14150 0.84 +1.3 1,363,461 + RPSI07_RS14150 0.84 +0.4 1,363,462 - RPSI07_RS14150 0.84 -1.9 1,363,510 - RPSI07_RS14150 0.88 +0.0 1,363,510 - RPSI07_RS14150 0.88 -1.4 1,363,566 + -1.5 1,363,567 - +0.1 1,363,590 + -1.3 1,363,590 + +0.8 1,363,630 - -1.3 1,363,850 + RPSI07_RS14155 0.15 -0.7 1,363,850 + RPSI07_RS14155 0.15 -0.6 1,363,851 - RPSI07_RS14155 0.16 -1.2 1,363,863 - RPSI07_RS14155 0.17 -0.8 1,363,944 - RPSI07_RS14155 0.24 -0.4 1,364,090 + RPSI07_RS14155 0.39 -0.4 1,364,090 + RPSI07_RS14155 0.39 +3.7 1,364,243 + +0.9 1,364,243 + RPSI07_RS14155 0.53 +0.9 1,364,389 + RPSI07_RS14155 0.67 -0.5 1,364,389 + RPSI07_RS14155 0.67 +1.3 1,364,390 - RPSI07_RS14155 0.67 +1.4 1,364,390 - RPSI07_RS14155 0.67 +0.5 1,364,468 + RPSI07_RS14155 0.75 -0.6 1,364,468 + RPSI07_RS14155 0.75 +0.0 1,364,469 - RPSI07_RS14155 0.75 +0.3 1,364,525 + RPSI07_RS14155 0.80 -0.4 1,364,528 + RPSI07_RS14155 0.81 +1.3 1,364,528 + RPSI07_RS14155 0.81 -1.0 1,364,529 - RPSI07_RS14155 0.81 -0.1
Or see this region's nucleotide sequence