Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS04740 and RPSI07_RS04745 are separated by 236 nucleotides RPSI07_RS04745 and RPSI07_RS04750 are separated by 364 nucleotides
RPSI07_RS04740: RPSI07_RS04740 - IMP dehydrogenase, at 1,285,323 to 1,288,769
_RS04740
RPSI07_RS04745: RPSI07_RS04745 - hypothetical protein, at 1,289,006 to 1,290,073
_RS04745
RPSI07_RS04750: RPSI07_RS04750 - DNA-binding protein, at 1,290,438 to 1,290,689
_RS04750
Position (kb)
1289
1290
1291 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1288.066 kb on + strand, within RPSI07_RS04740 at 1288.066 kb on + strand, within RPSI07_RS04740 at 1288.067 kb on - strand, within RPSI07_RS04740 at 1288.067 kb on - strand, within RPSI07_RS04740 at 1288.160 kb on - strand, within RPSI07_RS04740 at 1288.297 kb on + strand, within RPSI07_RS04740 at 1288.420 kb on + strand, within RPSI07_RS04740 at 1288.420 kb on + strand, within RPSI07_RS04740 at 1288.420 kb on + strand, within RPSI07_RS04740 at 1288.453 kb on + strand at 1288.453 kb on + strand at 1288.453 kb on + strand at 1288.507 kb on + strand at 1288.507 kb on + strand at 1288.508 kb on - strand at 1288.726 kb on + strand at 1288.726 kb on + strand at 1288.726 kb on + strand at 1288.727 kb on - strand at 1288.819 kb on - strand at 1288.985 kb on - strand at 1289.090 kb on + strand at 1289.198 kb on + strand, within RPSI07_RS04745 at 1289.198 kb on + strand, within RPSI07_RS04745 at 1289.198 kb on + strand, within RPSI07_RS04745 at 1289.198 kb on + strand, within RPSI07_RS04745 at 1289.198 kb on + strand, within RPSI07_RS04745 at 1289.199 kb on - strand, within RPSI07_RS04745 at 1289.271 kb on + strand, within RPSI07_RS04745 at 1289.315 kb on + strand, within RPSI07_RS04745 at 1289.315 kb on + strand, within RPSI07_RS04745 at 1289.316 kb on - strand, within RPSI07_RS04745 at 1289.316 kb on - strand, within RPSI07_RS04745 at 1289.354 kb on + strand, within RPSI07_RS04745 at 1289.355 kb on - strand, within RPSI07_RS04745 at 1289.355 kb on - strand, within RPSI07_RS04745 at 1289.356 kb on + strand, within RPSI07_RS04745 at 1289.357 kb on - strand, within RPSI07_RS04745 at 1289.594 kb on + strand, within RPSI07_RS04745 at 1289.594 kb on + strand, within RPSI07_RS04745 at 1289.594 kb on - strand, within RPSI07_RS04745 at 1289.595 kb on - strand, within RPSI07_RS04745 at 1289.595 kb on - strand, within RPSI07_RS04745 at 1289.595 kb on - strand, within RPSI07_RS04745 at 1289.694 kb on - strand, within RPSI07_RS04745 at 1289.696 kb on + strand, within RPSI07_RS04745 at 1289.744 kb on + strand, within RPSI07_RS04745 at 1289.792 kb on + strand, within RPSI07_RS04745 at 1289.792 kb on + strand, within RPSI07_RS04745 at 1289.793 kb on - strand, within RPSI07_RS04745 at 1289.809 kb on + strand, within RPSI07_RS04745 at 1289.942 kb on + strand, within RPSI07_RS04745 at 1289.942 kb on + strand, within RPSI07_RS04745 at 1289.942 kb on + strand, within RPSI07_RS04745 at 1289.942 kb on + strand, within RPSI07_RS04745 at 1289.942 kb on + strand, within RPSI07_RS04745 at 1289.942 kb on + strand, within RPSI07_RS04745 at 1289.943 kb on - strand, within RPSI07_RS04745 at 1289.943 kb on - strand, within RPSI07_RS04745 at 1290.071 kb on + strand at 1290.334 kb on + strand at 1290.335 kb on - strand at 1290.335 kb on - strand at 1290.335 kb on - strand at 1290.342 kb on + strand at 1290.342 kb on + strand at 1290.342 kb on + strand at 1290.342 kb on + strand at 1290.423 kb on + strand at 1290.423 kb on + strand at 1290.423 kb on + strand at 1290.423 kb on + strand at 1290.424 kb on - strand at 1290.424 kb on - strand at 1290.424 kb on - strand at 1290.424 kb on - strand at 1290.424 kb on - strand at 1290.424 kb on - strand at 1290.582 kb on + strand, within RPSI07_RS04750 at 1290.582 kb on + strand, within RPSI07_RS04750 at 1290.582 kb on + strand, within RPSI07_RS04750 at 1290.582 kb on + strand, within RPSI07_RS04750 at 1290.582 kb on + strand, within RPSI07_RS04750 at 1290.582 kb on + strand, within RPSI07_RS04750 at 1290.582 kb on + strand, within RPSI07_RS04750 at 1290.582 kb on + strand, within RPSI07_RS04750 at 1290.582 kb on + strand, within RPSI07_RS04750 at 1290.583 kb on - strand, within RPSI07_RS04750 at 1290.583 kb on - strand, within RPSI07_RS04750 at 1290.584 kb on + strand, within RPSI07_RS04750 at 1290.584 kb on + strand, within RPSI07_RS04750 at 1290.584 kb on + strand, within RPSI07_RS04750 at 1290.585 kb on - strand, within RPSI07_RS04750 at 1290.585 kb on - strand, within RPSI07_RS04750 at 1290.585 kb on - strand, within RPSI07_RS04750 at 1290.585 kb on - strand, within RPSI07_RS04750 at 1290.585 kb on - strand, within RPSI07_RS04750 at 1290.718 kb on - strand at 1290.719 kb on + strand at 1290.719 kb on + strand at 1290.720 kb on - strand at 1290.720 kb on - strand at 1291.071 kb on + strand at 1291.071 kb on + strand at 1291.072 kb on - strand at 1291.072 kb on - strand at 1291.072 kb on - strand at 1291.072 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 1,288,066 + RPSI07_RS04740 0.80 +1.5 1,288,066 + RPSI07_RS04740 0.80 -1.6 1,288,067 - RPSI07_RS04740 0.80 -0.5 1,288,067 - RPSI07_RS04740 0.80 -0.5 1,288,160 - RPSI07_RS04740 0.82 +0.7 1,288,297 + RPSI07_RS04740 0.86 -0.1 1,288,420 + RPSI07_RS04740 0.90 +0.7 1,288,420 + RPSI07_RS04740 0.90 +2.3 1,288,420 + RPSI07_RS04740 0.90 -1.4 1,288,453 + -0.3 1,288,453 + -0.1 1,288,453 + +1.6 1,288,507 + +0.7 1,288,507 + -0.7 1,288,508 - -1.7 1,288,726 + +0.7 1,288,726 + +0.2 1,288,726 + +1.7 1,288,727 - -0.5 1,288,819 - +0.1 1,288,985 - -0.4 1,289,090 + +3.5 1,289,198 + RPSI07_RS04745 0.18 -1.6 1,289,198 + RPSI07_RS04745 0.18 -0.8 1,289,198 + RPSI07_RS04745 0.18 -0.7 1,289,198 + RPSI07_RS04745 0.18 +0.7 1,289,198 + RPSI07_RS04745 0.18 +0.7 1,289,199 - RPSI07_RS04745 0.18 +0.0 1,289,271 + RPSI07_RS04745 0.25 -0.1 1,289,315 + RPSI07_RS04745 0.29 -1.1 1,289,315 + RPSI07_RS04745 0.29 -0.6 1,289,316 - RPSI07_RS04745 0.29 -1.0 1,289,316 - RPSI07_RS04745 0.29 -0.8 1,289,354 + RPSI07_RS04745 0.33 +0.5 1,289,355 - RPSI07_RS04745 0.33 -0.5 1,289,355 - RPSI07_RS04745 0.33 -0.2 1,289,356 + RPSI07_RS04745 0.33 -0.2 1,289,357 - RPSI07_RS04745 0.33 -0.3 1,289,594 + RPSI07_RS04745 0.55 +1.4 1,289,594 + RPSI07_RS04745 0.55 -0.7 1,289,594 - RPSI07_RS04745 0.55 -1.6 1,289,595 - RPSI07_RS04745 0.55 +0.6 1,289,595 - RPSI07_RS04745 0.55 +1.0 1,289,595 - RPSI07_RS04745 0.55 -1.1 1,289,694 - RPSI07_RS04745 0.64 -1.2 1,289,696 + RPSI07_RS04745 0.65 +0.7 1,289,744 + RPSI07_RS04745 0.69 -1.1 1,289,792 + RPSI07_RS04745 0.74 +2.1 1,289,792 + RPSI07_RS04745 0.74 -0.2 1,289,793 - RPSI07_RS04745 0.74 +0.2 1,289,809 + RPSI07_RS04745 0.75 +2.5 1,289,942 + RPSI07_RS04745 0.88 -1.5 1,289,942 + RPSI07_RS04745 0.88 +0.7 1,289,942 + RPSI07_RS04745 0.88 -1.1 1,289,942 + RPSI07_RS04745 0.88 +2.3 1,289,942 + RPSI07_RS04745 0.88 -0.1 1,289,942 + RPSI07_RS04745 0.88 +0.7 1,289,943 - RPSI07_RS04745 0.88 +0.3 1,289,943 - RPSI07_RS04745 0.88 -2.2 1,290,071 + -0.2 1,290,334 + +0.4 1,290,335 - +0.2 1,290,335 - -1.7 1,290,335 - +2.0 1,290,342 + -0.4 1,290,342 + -0.1 1,290,342 + +0.1 1,290,342 + +0.2 1,290,423 + -1.9 1,290,423 + -0.2 1,290,423 + +0.5 1,290,423 + -0.6 1,290,424 - +1.4 1,290,424 - +2.3 1,290,424 - +0.2 1,290,424 - +4.6 1,290,424 - +2.2 1,290,424 - +1.4 1,290,582 + RPSI07_RS04750 0.57 +2.3 1,290,582 + RPSI07_RS04750 0.57 +1.3 1,290,582 + RPSI07_RS04750 0.57 +3.1 1,290,582 + RPSI07_RS04750 0.57 -0.6 1,290,582 + RPSI07_RS04750 0.57 +1.4 1,290,582 + RPSI07_RS04750 0.57 +0.4 1,290,582 + RPSI07_RS04750 0.57 +0.5 1,290,582 + RPSI07_RS04750 0.57 +1.4 1,290,582 + RPSI07_RS04750 0.57 -0.3 1,290,583 - RPSI07_RS04750 0.58 +1.0 1,290,583 - RPSI07_RS04750 0.58 -0.5 1,290,584 + RPSI07_RS04750 0.58 -0.5 1,290,584 + RPSI07_RS04750 0.58 -0.5 1,290,584 + RPSI07_RS04750 0.58 +0.7 1,290,585 - RPSI07_RS04750 0.58 +1.7 1,290,585 - RPSI07_RS04750 0.58 +2.2 1,290,585 - RPSI07_RS04750 0.58 +1.5 1,290,585 - RPSI07_RS04750 0.58 +1.8 1,290,585 - RPSI07_RS04750 0.58 +1.2 1,290,718 - -0.1 1,290,719 + +0.4 1,290,719 + -0.3 1,290,720 - +0.7 1,290,720 - -0.3 1,291,071 + -1.9 1,291,071 + +1.5 1,291,072 - +0.0 1,291,072 - -1.0 1,291,072 - +0.8 1,291,072 - +0.7
Or see this region's nucleotide sequence