Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS03240 and RPSI07_RS03245 are separated by 193 nucleotides RPSI07_RS03245 and RPSI07_RS03250 are separated by 53 nucleotides
RPSI07_RS03240: RPSI07_RS03240 - LysR family transcriptional regulator, at 884,935 to 885,873
_RS03240
RPSI07_RS03245: RPSI07_RS03245 - ABC transporter substrate-binding protein, at 886,067 to 887,611
_RS03245
RPSI07_RS03250: RPSI07_RS03250 - ABC transporter permease, at 887,665 to 888,582
_RS03250
Position (kb) 886
887
888 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 885.182 kb on + strand, within RPSI07_RS03240 at 885.183 kb on - strand, within RPSI07_RS03240 at 885.183 kb on - strand, within RPSI07_RS03240 at 885.183 kb on - strand, within RPSI07_RS03240 at 885.183 kb on - strand, within RPSI07_RS03240 at 885.410 kb on + strand, within RPSI07_RS03240 at 885.411 kb on - strand, within RPSI07_RS03240 at 885.411 kb on - strand, within RPSI07_RS03240 at 885.411 kb on - strand, within RPSI07_RS03240 at 885.411 kb on - strand, within RPSI07_RS03240 at 885.506 kb on + strand, within RPSI07_RS03240 at 885.507 kb on - strand, within RPSI07_RS03240 at 885.507 kb on - strand, within RPSI07_RS03240 at 885.507 kb on - strand, within RPSI07_RS03240 at 885.507 kb on - strand, within RPSI07_RS03240 at 885.507 kb on - strand, within RPSI07_RS03240 at 885.507 kb on - strand, within RPSI07_RS03240 at 885.833 kb on + strand at 885.833 kb on + strand at 885.893 kb on + strand at 885.894 kb on - strand at 885.894 kb on - strand at 885.894 kb on - strand at 885.956 kb on + strand at 885.984 kb on + strand at 885.984 kb on + strand at 885.992 kb on + strand at 885.993 kb on - strand at 885.993 kb on - strand at 886.010 kb on + strand at 886.010 kb on + strand at 886.011 kb on - strand at 886.066 kb on + strand at 886.066 kb on + strand at 886.067 kb on - strand at 886.185 kb on - strand at 886.270 kb on + strand, within RPSI07_RS03245 at 886.271 kb on - strand, within RPSI07_RS03245 at 886.304 kb on + strand, within RPSI07_RS03245 at 886.433 kb on + strand, within RPSI07_RS03245 at 886.433 kb on + strand, within RPSI07_RS03245 at 886.433 kb on + strand, within RPSI07_RS03245 at 886.433 kb on + strand, within RPSI07_RS03245 at 886.433 kb on + strand, within RPSI07_RS03245 at 886.433 kb on + strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.434 kb on - strand, within RPSI07_RS03245 at 886.559 kb on + strand, within RPSI07_RS03245 at 886.559 kb on + strand, within RPSI07_RS03245 at 886.560 kb on - strand, within RPSI07_RS03245 at 886.560 kb on - strand, within RPSI07_RS03245 at 886.560 kb on - strand, within RPSI07_RS03245 at 886.560 kb on - strand, within RPSI07_RS03245 at 886.652 kb on + strand, within RPSI07_RS03245 at 886.652 kb on + strand, within RPSI07_RS03245 at 886.653 kb on - strand, within RPSI07_RS03245 at 886.653 kb on - strand, within RPSI07_RS03245 at 886.653 kb on - strand, within RPSI07_RS03245 at 886.709 kb on + strand, within RPSI07_RS03245 at 886.709 kb on + strand, within RPSI07_RS03245 at 886.710 kb on - strand, within RPSI07_RS03245 at 886.710 kb on - strand, within RPSI07_RS03245 at 886.710 kb on - strand, within RPSI07_RS03245 at 886.985 kb on + strand, within RPSI07_RS03245 at 886.986 kb on - strand, within RPSI07_RS03245 at 886.986 kb on - strand, within RPSI07_RS03245 at 887.057 kb on + strand, within RPSI07_RS03245 at 887.057 kb on + strand, within RPSI07_RS03245 at 887.105 kb on + strand, within RPSI07_RS03245 at 887.106 kb on - strand, within RPSI07_RS03245 at 887.174 kb on + strand, within RPSI07_RS03245 at 887.174 kb on + strand, within RPSI07_RS03245 at 887.174 kb on + strand, within RPSI07_RS03245 at 887.174 kb on + strand, within RPSI07_RS03245 at 887.174 kb on + strand, within RPSI07_RS03245 at 887.174 kb on + strand, within RPSI07_RS03245 at 887.175 kb on - strand, within RPSI07_RS03245 at 887.175 kb on - strand, within RPSI07_RS03245 at 887.175 kb on - strand, within RPSI07_RS03245 at 887.175 kb on - strand, within RPSI07_RS03245 at 887.175 kb on - strand, within RPSI07_RS03245 at 887.175 kb on - strand, within RPSI07_RS03245 at 887.175 kb on - strand, within RPSI07_RS03245 at 887.175 kb on - strand, within RPSI07_RS03245 at 887.175 kb on - strand, within RPSI07_RS03245 at 887.180 kb on + strand, within RPSI07_RS03245 at 887.181 kb on - strand, within RPSI07_RS03245 at 887.252 kb on + strand, within RPSI07_RS03245 at 887.252 kb on + strand, within RPSI07_RS03245 at 887.252 kb on + strand, within RPSI07_RS03245 at 887.253 kb on - strand, within RPSI07_RS03245 at 887.253 kb on - strand, within RPSI07_RS03245 at 887.261 kb on + strand, within RPSI07_RS03245 at 887.261 kb on + strand, within RPSI07_RS03245 at 887.261 kb on + strand, within RPSI07_RS03245 at 887.262 kb on - strand, within RPSI07_RS03245 at 887.262 kb on - strand, within RPSI07_RS03245 at 887.263 kb on - strand, within RPSI07_RS03245 at 887.414 kb on + strand, within RPSI07_RS03245 at 887.414 kb on + strand, within RPSI07_RS03245 at 887.414 kb on + strand, within RPSI07_RS03245 at 887.415 kb on - strand, within RPSI07_RS03245 at 887.415 kb on - strand, within RPSI07_RS03245 at 887.466 kb on - strand at 887.466 kb on - strand at 887.466 kb on - strand at 887.522 kb on + strand at 887.523 kb on - strand at 887.523 kb on - strand at 887.523 kb on - strand at 887.534 kb on + strand at 887.534 kb on + strand at 887.534 kb on + strand at 887.534 kb on + strand at 887.534 kb on + strand at 887.535 kb on - strand at 887.535 kb on - strand at 887.540 kb on + strand at 887.541 kb on - strand at 887.541 kb on - strand at 887.541 kb on - strand at 887.568 kb on - strand at 887.591 kb on + strand at 887.592 kb on - strand at 887.609 kb on + strand at 887.610 kb on - strand at 887.674 kb on + strand at 887.674 kb on + strand at 887.674 kb on + strand at 887.674 kb on + strand at 887.675 kb on - strand at 887.675 kb on - strand at 887.675 kb on - strand at 887.675 kb on - strand at 887.675 kb on - strand at 887.675 kb on - strand at 887.675 kb on - strand at 887.675 kb on - strand at 887.825 kb on - strand, within RPSI07_RS03250 at 887.854 kb on + strand, within RPSI07_RS03250 at 887.855 kb on - strand, within RPSI07_RS03250 at 888.028 kb on + strand, within RPSI07_RS03250 at 888.205 kb on + strand, within RPSI07_RS03250 at 888.205 kb on + strand, within RPSI07_RS03250 at 888.206 kb on - strand, within RPSI07_RS03250 at 888.208 kb on + strand, within RPSI07_RS03250 at 888.208 kb on + strand, within RPSI07_RS03250 at 888.271 kb on + strand, within RPSI07_RS03250 at 888.271 kb on + strand, within RPSI07_RS03250 at 888.272 kb on - strand, within RPSI07_RS03250 at 888.418 kb on + strand, within RPSI07_RS03250 at 888.418 kb on + strand, within RPSI07_RS03250 at 888.418 kb on + strand, within RPSI07_RS03250 at 888.418 kb on + strand, within RPSI07_RS03250 at 888.419 kb on - strand, within RPSI07_RS03250 at 888.514 kb on + strand at 888.514 kb on + strand at 888.514 kb on + strand at 888.514 kb on + strand at 888.514 kb on + strand at 888.514 kb on + strand at 888.514 kb on + strand at 888.514 kb on + strand at 888.515 kb on - strand at 888.515 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_CrystalViolet remove 885,182 + RPSI07_RS03240 0.26 -0.8 885,183 - RPSI07_RS03240 0.26 +1.5 885,183 - RPSI07_RS03240 0.26 -2.0 885,183 - RPSI07_RS03240 0.26 +0.3 885,183 - RPSI07_RS03240 0.26 -1.8 885,410 + RPSI07_RS03240 0.51 -0.9 885,411 - RPSI07_RS03240 0.51 -0.3 885,411 - RPSI07_RS03240 0.51 -1.3 885,411 - RPSI07_RS03240 0.51 -0.7 885,411 - RPSI07_RS03240 0.51 +0.7 885,506 + RPSI07_RS03240 0.61 +0.5 885,507 - RPSI07_RS03240 0.61 +1.9 885,507 - RPSI07_RS03240 0.61 +0.5 885,507 - RPSI07_RS03240 0.61 -1.1 885,507 - RPSI07_RS03240 0.61 +0.9 885,507 - RPSI07_RS03240 0.61 +0.2 885,507 - RPSI07_RS03240 0.61 +0.2 885,833 + -0.5 885,833 + -0.4 885,893 + -0.2 885,894 - -1.7 885,894 - +0.2 885,894 - -2.7 885,956 + +0.4 885,984 + -1.3 885,984 + -3.7 885,992 + +1.1 885,993 - -1.0 885,993 - -1.1 886,010 + -0.8 886,010 + +0.7 886,011 - -1.5 886,066 + +0.9 886,066 + -0.5 886,067 - -0.1 886,185 - +0.7 886,270 + RPSI07_RS03245 0.13 -0.5 886,271 - RPSI07_RS03245 0.13 -0.6 886,304 + RPSI07_RS03245 0.15 +2.3 886,433 + RPSI07_RS03245 0.24 -0.1 886,433 + RPSI07_RS03245 0.24 +1.2 886,433 + RPSI07_RS03245 0.24 -1.4 886,433 + RPSI07_RS03245 0.24 +3.7 886,433 + RPSI07_RS03245 0.24 -1.3 886,433 + RPSI07_RS03245 0.24 -0.3 886,434 - RPSI07_RS03245 0.24 +0.3 886,434 - RPSI07_RS03245 0.24 -0.7 886,434 - RPSI07_RS03245 0.24 -0.5 886,434 - RPSI07_RS03245 0.24 -0.7 886,434 - RPSI07_RS03245 0.24 +1.3 886,434 - RPSI07_RS03245 0.24 -0.0 886,434 - RPSI07_RS03245 0.24 -0.1 886,434 - RPSI07_RS03245 0.24 -2.3 886,434 - RPSI07_RS03245 0.24 +0.3 886,559 + RPSI07_RS03245 0.32 -0.8 886,559 + RPSI07_RS03245 0.32 -0.7 886,560 - RPSI07_RS03245 0.32 +0.2 886,560 - RPSI07_RS03245 0.32 +0.5 886,560 - RPSI07_RS03245 0.32 +0.1 886,560 - RPSI07_RS03245 0.32 -0.4 886,652 + RPSI07_RS03245 0.38 +2.1 886,652 + RPSI07_RS03245 0.38 +0.7 886,653 - RPSI07_RS03245 0.38 -0.0 886,653 - RPSI07_RS03245 0.38 +1.1 886,653 - RPSI07_RS03245 0.38 +0.4 886,709 + RPSI07_RS03245 0.42 -0.8 886,709 + RPSI07_RS03245 0.42 -0.5 886,710 - RPSI07_RS03245 0.42 -0.6 886,710 - RPSI07_RS03245 0.42 -2.3 886,710 - RPSI07_RS03245 0.42 -0.9 886,985 + RPSI07_RS03245 0.59 +0.7 886,986 - RPSI07_RS03245 0.59 -0.6 886,986 - RPSI07_RS03245 0.59 +0.1 887,057 + RPSI07_RS03245 0.64 +1.1 887,057 + RPSI07_RS03245 0.64 -0.6 887,105 + RPSI07_RS03245 0.67 -0.0 887,106 - RPSI07_RS03245 0.67 -2.1 887,174 + RPSI07_RS03245 0.72 +1.3 887,174 + RPSI07_RS03245 0.72 +0.6 887,174 + RPSI07_RS03245 0.72 -0.7 887,174 + RPSI07_RS03245 0.72 -0.3 887,174 + RPSI07_RS03245 0.72 +0.4 887,174 + RPSI07_RS03245 0.72 -1.6 887,175 - RPSI07_RS03245 0.72 -1.6 887,175 - RPSI07_RS03245 0.72 -1.4 887,175 - RPSI07_RS03245 0.72 -0.1 887,175 - RPSI07_RS03245 0.72 +2.3 887,175 - RPSI07_RS03245 0.72 +0.3 887,175 - RPSI07_RS03245 0.72 -1.8 887,175 - RPSI07_RS03245 0.72 -1.1 887,175 - RPSI07_RS03245 0.72 -0.2 887,175 - RPSI07_RS03245 0.72 -0.2 887,180 + RPSI07_RS03245 0.72 +0.7 887,181 - RPSI07_RS03245 0.72 -1.2 887,252 + RPSI07_RS03245 0.77 -0.7 887,252 + RPSI07_RS03245 0.77 +1.7 887,252 + RPSI07_RS03245 0.77 -1.2 887,253 - RPSI07_RS03245 0.77 +1.0 887,253 - RPSI07_RS03245 0.77 +0.5 887,261 + RPSI07_RS03245 0.77 -1.4 887,261 + RPSI07_RS03245 0.77 -0.6 887,261 + RPSI07_RS03245 0.77 +1.5 887,262 - RPSI07_RS03245 0.77 +0.1 887,262 - RPSI07_RS03245 0.77 +1.2 887,263 - RPSI07_RS03245 0.77 -0.1 887,414 + RPSI07_RS03245 0.87 -1.5 887,414 + RPSI07_RS03245 0.87 +0.7 887,414 + RPSI07_RS03245 0.87 -1.4 887,415 - RPSI07_RS03245 0.87 +0.7 887,415 - RPSI07_RS03245 0.87 +2.1 887,466 - -1.3 887,466 - +2.5 887,466 - +0.7 887,522 + +1.7 887,523 - -0.3 887,523 - -2.3 887,523 - -0.9 887,534 + +2.5 887,534 + +0.5 887,534 + -0.1 887,534 + -1.9 887,534 + -0.9 887,535 - -2.3 887,535 - -0.0 887,540 + +1.7 887,541 - -0.7 887,541 - +1.1 887,541 - -0.2 887,568 - +0.9 887,591 + +0.8 887,592 - +0.1 887,609 + -1.1 887,610 - +2.3 887,674 + +1.0 887,674 + +1.1 887,674 + -1.8 887,674 + -1.0 887,675 - -0.7 887,675 - -0.9 887,675 - +0.6 887,675 - -0.0 887,675 - -0.9 887,675 - +1.3 887,675 - +1.1 887,675 - -1.0 887,825 - RPSI07_RS03250 0.17 +0.8 887,854 + RPSI07_RS03250 0.21 -0.1 887,855 - RPSI07_RS03250 0.21 -1.6 888,028 + RPSI07_RS03250 0.40 -0.1 888,205 + RPSI07_RS03250 0.59 -0.7 888,205 + RPSI07_RS03250 0.59 +0.1 888,206 - RPSI07_RS03250 0.59 +0.1 888,208 + RPSI07_RS03250 0.59 -0.9 888,208 + RPSI07_RS03250 0.59 +0.1 888,271 + RPSI07_RS03250 0.66 -1.1 888,271 + RPSI07_RS03250 0.66 -0.1 888,272 - RPSI07_RS03250 0.66 +0.7 888,418 + RPSI07_RS03250 0.82 +1.1 888,418 + RPSI07_RS03250 0.82 -0.7 888,418 + RPSI07_RS03250 0.82 +0.7 888,418 + RPSI07_RS03250 0.82 +2.7 888,419 - RPSI07_RS03250 0.82 -0.1 888,514 + +0.7 888,514 + -1.2 888,514 + +0.1 888,514 + +2.1 888,514 + +0.8 888,514 + +1.2 888,514 + -0.3 888,514 + +2.7 888,515 - -2.3 888,515 - -4.0
Or see this region's nucleotide sequence