Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS24680 and RPSI07_RS06905 are separated by 123 nucleotides RPSI07_RS06905 and RPSI07_RS24685 are separated by 186 nucleotides RPSI07_RS24685 and RPSI07_RS06910 are separated by 181 nucleotides RPSI07_RS06910 and RPSI07_RS24690 are separated by 422 nucleotides
RPSI07_RS24680: RPSI07_RS24680 - hypothetical protein, at 1,839,746 to 1,839,937
_RS24680
RPSI07_RS06905: RPSI07_RS06905 - DUF2147 domain-containing protein, at 1,840,061 to 1,840,531
_RS06905
RPSI07_RS24685: RPSI07_RS24685 - hypothetical protein, at 1,840,718 to 1,841,050
_RS24685
RPSI07_RS06910: RPSI07_RS06910 - hypothetical protein, at 1,841,232 to 1,841,510
_RS06910
RPSI07_RS24690: RPSI07_RS24690 - hypothetical protein, at 1,841,933 to 1,842,319
_RS24690
Position (kb)
1840
1841
1842 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1839.804 kb on + strand, within RPSI07_RS24680 at 1839.804 kb on + strand, within RPSI07_RS24680 at 1839.804 kb on + strand, within RPSI07_RS24680 at 1839.804 kb on + strand, within RPSI07_RS24680 at 1839.805 kb on - strand, within RPSI07_RS24680 at 1839.805 kb on - strand, within RPSI07_RS24680 at 1839.805 kb on - strand, within RPSI07_RS24680 at 1839.805 kb on - strand, within RPSI07_RS24680 at 1839.805 kb on - strand, within RPSI07_RS24680 at 1839.805 kb on - strand, within RPSI07_RS24680 at 1839.805 kb on - strand, within RPSI07_RS24680 at 1839.896 kb on + strand, within RPSI07_RS24680 at 1839.896 kb on + strand, within RPSI07_RS24680 at 1839.896 kb on + strand, within RPSI07_RS24680 at 1839.896 kb on + strand, within RPSI07_RS24680 at 1839.896 kb on + strand, within RPSI07_RS24680 at 1839.896 kb on + strand, within RPSI07_RS24680 at 1839.896 kb on + strand, within RPSI07_RS24680 at 1839.896 kb on + strand, within RPSI07_RS24680 at 1839.897 kb on - strand, within RPSI07_RS24680 at 1839.897 kb on - strand, within RPSI07_RS24680 at 1839.897 kb on - strand, within RPSI07_RS24680 at 1839.897 kb on - strand, within RPSI07_RS24680 at 1839.897 kb on - strand, within RPSI07_RS24680 at 1839.897 kb on - strand, within RPSI07_RS24680 at 1839.903 kb on + strand, within RPSI07_RS24680 at 1839.904 kb on - strand, within RPSI07_RS24680 at 1839.911 kb on + strand, within RPSI07_RS24680 at 1839.933 kb on + strand at 1839.933 kb on + strand at 1839.933 kb on + strand at 1839.933 kb on + strand at 1839.933 kb on + strand at 1839.933 kb on + strand at 1839.934 kb on - strand at 1839.934 kb on - strand at 1839.934 kb on - strand at 1839.999 kb on + strand at 1840.027 kb on - strand at 1840.074 kb on + strand at 1840.075 kb on - strand at 1840.134 kb on + strand, within RPSI07_RS06905 at 1840.134 kb on + strand, within RPSI07_RS06905 at 1840.137 kb on + strand, within RPSI07_RS06905 at 1840.137 kb on + strand, within RPSI07_RS06905 at 1840.137 kb on + strand, within RPSI07_RS06905 at 1840.137 kb on + strand, within RPSI07_RS06905 at 1840.138 kb on - strand, within RPSI07_RS06905 at 1840.138 kb on - strand, within RPSI07_RS06905 at 1840.138 kb on - strand, within RPSI07_RS06905 at 1840.155 kb on + strand, within RPSI07_RS06905 at 1840.156 kb on - strand, within RPSI07_RS06905 at 1840.156 kb on - strand, within RPSI07_RS06905 at 1840.156 kb on - strand, within RPSI07_RS06905 at 1840.156 kb on - strand, within RPSI07_RS06905 at 1840.217 kb on + strand, within RPSI07_RS06905 at 1840.217 kb on + strand, within RPSI07_RS06905 at 1840.217 kb on + strand, within RPSI07_RS06905 at 1840.217 kb on + strand, within RPSI07_RS06905 at 1840.217 kb on + strand, within RPSI07_RS06905 at 1840.217 kb on + strand, within RPSI07_RS06905 at 1840.218 kb on - strand, within RPSI07_RS06905 at 1840.218 kb on - strand, within RPSI07_RS06905 at 1840.400 kb on + strand, within RPSI07_RS06905 at 1840.401 kb on + strand, within RPSI07_RS06905 at 1840.401 kb on + strand, within RPSI07_RS06905 at 1840.401 kb on + strand, within RPSI07_RS06905 at 1840.401 kb on + strand, within RPSI07_RS06905 at 1840.401 kb on + strand, within RPSI07_RS06905 at 1840.401 kb on + strand, within RPSI07_RS06905 at 1840.402 kb on - strand, within RPSI07_RS06905 at 1840.402 kb on - strand, within RPSI07_RS06905 at 1840.402 kb on - strand, within RPSI07_RS06905 at 1840.402 kb on - strand, within RPSI07_RS06905 at 1840.402 kb on - strand, within RPSI07_RS06905 at 1840.402 kb on - strand, within RPSI07_RS06905 at 1840.402 kb on - strand, within RPSI07_RS06905 at 1840.403 kb on + strand, within RPSI07_RS06905 at 1840.403 kb on + strand, within RPSI07_RS06905 at 1840.403 kb on + strand, within RPSI07_RS06905 at 1840.403 kb on + strand, within RPSI07_RS06905 at 1840.403 kb on + strand, within RPSI07_RS06905 at 1840.404 kb on - strand, within RPSI07_RS06905 at 1840.404 kb on - strand, within RPSI07_RS06905 at 1840.404 kb on - strand, within RPSI07_RS06905 at 1840.404 kb on - strand, within RPSI07_RS06905 at 1840.405 kb on - strand, within RPSI07_RS06905 at 1840.428 kb on - strand, within RPSI07_RS06905 at 1840.428 kb on - strand, within RPSI07_RS06905 at 1840.428 kb on - strand, within RPSI07_RS06905 at 1840.475 kb on + strand, within RPSI07_RS06905 at 1840.476 kb on - strand, within RPSI07_RS06905 at 1840.560 kb on + strand at 1840.560 kb on + strand at 1840.560 kb on + strand at 1840.560 kb on + strand at 1840.560 kb on + strand at 1840.561 kb on - strand at 1840.561 kb on - strand at 1840.561 kb on - strand at 1840.561 kb on - strand at 1840.561 kb on - strand at 1840.571 kb on + strand at 1840.571 kb on + strand at 1840.572 kb on - strand at 1840.572 kb on - strand at 1840.572 kb on - strand at 1840.572 kb on - strand at 1840.572 kb on - strand at 1840.572 kb on - strand at 1840.573 kb on - strand at 1840.580 kb on + strand at 1840.580 kb on + strand at 1840.580 kb on + strand at 1840.580 kb on + strand at 1840.581 kb on - strand at 1840.581 kb on - strand at 1840.581 kb on - strand at 1840.581 kb on - strand at 1840.602 kb on + strand at 1840.603 kb on - strand at 1840.603 kb on - strand at 1840.603 kb on - strand at 1840.603 kb on - strand at 1840.766 kb on - strand, within RPSI07_RS24685 at 1840.766 kb on - strand, within RPSI07_RS24685 at 1840.915 kb on + strand, within RPSI07_RS24685 at 1840.945 kb on + strand, within RPSI07_RS24685 at 1841.044 kb on + strand at 1841.045 kb on - strand at 1841.076 kb on + strand at 1841.150 kb on + strand at 1841.150 kb on + strand at 1841.151 kb on - strand at 1841.151 kb on - strand at 1841.151 kb on - strand at 1841.151 kb on - strand at 1841.151 kb on - strand at 1841.151 kb on - strand at 1841.151 kb on - strand at 1841.151 kb on - strand at 1841.164 kb on + strand at 1841.165 kb on - strand at 1841.165 kb on - strand at 1841.410 kb on + strand, within RPSI07_RS06910 at 1841.411 kb on - strand, within RPSI07_RS06910 at 1841.443 kb on - strand, within RPSI07_RS06910 at 1841.443 kb on - strand, within RPSI07_RS06910 at 1841.443 kb on - strand, within RPSI07_RS06910 at 1841.562 kb on + strand at 1841.663 kb on - strand at 1841.663 kb on - strand at 1841.663 kb on - strand at 1841.663 kb on - strand at 1841.663 kb on - strand at 1841.673 kb on + strand at 1841.673 kb on + strand at 1841.674 kb on - strand at 1841.674 kb on - strand at 1841.674 kb on - strand at 1841.703 kb on - strand at 1841.727 kb on - strand at 1841.727 kb on - strand at 1841.730 kb on + strand at 1841.731 kb on - strand at 1841.778 kb on - strand at 1841.778 kb on - strand at 1841.823 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 1,839,804 + RPSI07_RS24680 0.30 -0.9 1,839,804 + RPSI07_RS24680 0.30 -2.0 1,839,804 + RPSI07_RS24680 0.30 -0.4 1,839,804 + RPSI07_RS24680 0.30 -0.5 1,839,805 - RPSI07_RS24680 0.31 -1.3 1,839,805 - RPSI07_RS24680 0.31 -1.2 1,839,805 - RPSI07_RS24680 0.31 +1.9 1,839,805 - RPSI07_RS24680 0.31 -0.9 1,839,805 - RPSI07_RS24680 0.31 +1.1 1,839,805 - RPSI07_RS24680 0.31 +1.6 1,839,805 - RPSI07_RS24680 0.31 -0.3 1,839,896 + RPSI07_RS24680 0.78 +2.7 1,839,896 + RPSI07_RS24680 0.78 -0.4 1,839,896 + RPSI07_RS24680 0.78 +0.2 1,839,896 + RPSI07_RS24680 0.78 -0.3 1,839,896 + RPSI07_RS24680 0.78 +1.2 1,839,896 + RPSI07_RS24680 0.78 +1.6 1,839,896 + RPSI07_RS24680 0.78 -0.7 1,839,896 + RPSI07_RS24680 0.78 +0.4 1,839,897 - RPSI07_RS24680 0.79 +2.0 1,839,897 - RPSI07_RS24680 0.79 -0.6 1,839,897 - RPSI07_RS24680 0.79 +0.7 1,839,897 - RPSI07_RS24680 0.79 +0.4 1,839,897 - RPSI07_RS24680 0.79 -0.4 1,839,897 - RPSI07_RS24680 0.79 +0.8 1,839,903 + RPSI07_RS24680 0.82 +2.6 1,839,904 - RPSI07_RS24680 0.82 -0.3 1,839,911 + RPSI07_RS24680 0.86 -0.2 1,839,933 + -0.3 1,839,933 + -0.5 1,839,933 + +0.2 1,839,933 + -2.4 1,839,933 + -0.1 1,839,933 + +1.6 1,839,934 - -0.2 1,839,934 - -2.0 1,839,934 - -2.4 1,839,999 + +0.5 1,840,027 - +1.1 1,840,074 + +2.1 1,840,075 - -1.0 1,840,134 + RPSI07_RS06905 0.15 +2.6 1,840,134 + RPSI07_RS06905 0.15 -0.2 1,840,137 + RPSI07_RS06905 0.16 -2.2 1,840,137 + RPSI07_RS06905 0.16 +0.6 1,840,137 + RPSI07_RS06905 0.16 +1.6 1,840,137 + RPSI07_RS06905 0.16 -0.1 1,840,138 - RPSI07_RS06905 0.16 -0.3 1,840,138 - RPSI07_RS06905 0.16 +1.0 1,840,138 - RPSI07_RS06905 0.16 -1.0 1,840,155 + RPSI07_RS06905 0.20 +0.2 1,840,156 - RPSI07_RS06905 0.20 +0.6 1,840,156 - RPSI07_RS06905 0.20 -2.4 1,840,156 - RPSI07_RS06905 0.20 -1.7 1,840,156 - RPSI07_RS06905 0.20 +1.6 1,840,217 + RPSI07_RS06905 0.33 -1.0 1,840,217 + RPSI07_RS06905 0.33 -1.5 1,840,217 + RPSI07_RS06905 0.33 -0.8 1,840,217 + RPSI07_RS06905 0.33 +0.1 1,840,217 + RPSI07_RS06905 0.33 +1.8 1,840,217 + RPSI07_RS06905 0.33 +0.6 1,840,218 - RPSI07_RS06905 0.33 +0.4 1,840,218 - RPSI07_RS06905 0.33 +0.3 1,840,400 + RPSI07_RS06905 0.72 -0.4 1,840,401 + RPSI07_RS06905 0.72 -2.0 1,840,401 + RPSI07_RS06905 0.72 -1.5 1,840,401 + RPSI07_RS06905 0.72 -0.4 1,840,401 + RPSI07_RS06905 0.72 +0.6 1,840,401 + RPSI07_RS06905 0.72 +0.6 1,840,401 + RPSI07_RS06905 0.72 +0.2 1,840,402 - RPSI07_RS06905 0.72 +0.9 1,840,402 - RPSI07_RS06905 0.72 -1.0 1,840,402 - RPSI07_RS06905 0.72 +0.8 1,840,402 - RPSI07_RS06905 0.72 +0.4 1,840,402 - RPSI07_RS06905 0.72 +3.6 1,840,402 - RPSI07_RS06905 0.72 +0.7 1,840,402 - RPSI07_RS06905 0.72 -1.6 1,840,403 + RPSI07_RS06905 0.73 +3.1 1,840,403 + RPSI07_RS06905 0.73 +0.6 1,840,403 + RPSI07_RS06905 0.73 +1.3 1,840,403 + RPSI07_RS06905 0.73 -3.8 1,840,403 + RPSI07_RS06905 0.73 +0.5 1,840,404 - RPSI07_RS06905 0.73 +0.2 1,840,404 - RPSI07_RS06905 0.73 -0.7 1,840,404 - RPSI07_RS06905 0.73 -1.9 1,840,404 - RPSI07_RS06905 0.73 -0.6 1,840,405 - RPSI07_RS06905 0.73 -1.7 1,840,428 - RPSI07_RS06905 0.78 +0.2 1,840,428 - RPSI07_RS06905 0.78 +3.7 1,840,428 - RPSI07_RS06905 0.78 -0.3 1,840,475 + RPSI07_RS06905 0.88 +0.0 1,840,476 - RPSI07_RS06905 0.88 -0.4 1,840,560 + -0.3 1,840,560 + +0.1 1,840,560 + -1.6 1,840,560 + +1.3 1,840,560 + -0.2 1,840,561 - -0.4 1,840,561 - +1.1 1,840,561 - -2.8 1,840,561 - +0.6 1,840,561 - -0.7 1,840,571 + -0.6 1,840,571 + +1.6 1,840,572 - +3.6 1,840,572 - +0.4 1,840,572 - +1.1 1,840,572 - -0.5 1,840,572 - -2.1 1,840,572 - -1.9 1,840,573 - -0.4 1,840,580 + +1.2 1,840,580 + -0.8 1,840,580 + -1.0 1,840,580 + +0.3 1,840,581 - +0.7 1,840,581 - -1.2 1,840,581 - +1.2 1,840,581 - -0.9 1,840,602 + +0.5 1,840,603 - -0.1 1,840,603 - -1.3 1,840,603 - -0.3 1,840,603 - -1.5 1,840,766 - RPSI07_RS24685 0.14 +0.3 1,840,766 - RPSI07_RS24685 0.14 +0.6 1,840,915 + RPSI07_RS24685 0.59 -0.8 1,840,945 + RPSI07_RS24685 0.68 +1.0 1,841,044 + -0.4 1,841,045 - +0.2 1,841,076 + +1.1 1,841,150 + +0.9 1,841,150 + -1.1 1,841,151 - -1.4 1,841,151 - +0.6 1,841,151 - +0.7 1,841,151 - +0.7 1,841,151 - +0.1 1,841,151 - -1.2 1,841,151 - -2.4 1,841,151 - -0.4 1,841,164 + +0.2 1,841,165 - -0.5 1,841,165 - -0.0 1,841,410 + RPSI07_RS06910 0.64 -2.0 1,841,411 - RPSI07_RS06910 0.64 +1.9 1,841,443 - RPSI07_RS06910 0.76 +0.7 1,841,443 - RPSI07_RS06910 0.76 +1.3 1,841,443 - RPSI07_RS06910 0.76 -0.5 1,841,562 + -0.4 1,841,663 - -0.9 1,841,663 - +0.5 1,841,663 - +0.6 1,841,663 - -1.4 1,841,663 - +0.6 1,841,673 + +0.8 1,841,673 + -1.9 1,841,674 - -0.4 1,841,674 - +1.8 1,841,674 - +0.6 1,841,703 - -0.1 1,841,727 - -0.4 1,841,727 - +1.0 1,841,730 + -1.6 1,841,731 - -0.9 1,841,778 - -0.1 1,841,778 - +1.4 1,841,823 - +1.6
Or see this region's nucleotide sequence