Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS17800 and RPSI07_RS17805 are separated by 199 nucleotides RPSI07_RS17805 and RPSI07_RS17810 are separated by 172 nucleotides RPSI07_RS17810 and RPSI07_RS17815 are separated by 100 nucleotides
RPSI07_RS17800: RPSI07_RS17800 - sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB, at 2,197,893 to 2,199,209
_RS17800
RPSI07_RS17805: RPSI07_RS17805 - HAD family hydrolase, at 2,199,409 to 2,200,296
_RS17805
RPSI07_RS17810: RPSI07_RS17810 - hypothetical protein, at 2,200,469 to 2,200,795
_RS17810
RPSI07_RS17815: RPSI07_RS17815 - nitrogen regulation protein NR(I), at 2,200,896 to 2,202,407
_RS17815
Position (kb)
2199
2200
2201 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2198.464 kb on + strand, within RPSI07_RS17800 at 2198.474 kb on - strand, within RPSI07_RS17800 at 2198.474 kb on - strand, within RPSI07_RS17800 at 2198.474 kb on - strand, within RPSI07_RS17800 at 2198.474 kb on - strand, within RPSI07_RS17800 at 2198.508 kb on - strand, within RPSI07_RS17800 at 2198.508 kb on - strand, within RPSI07_RS17800 at 2198.645 kb on - strand, within RPSI07_RS17800 at 2198.758 kb on + strand, within RPSI07_RS17800 at 2198.758 kb on + strand, within RPSI07_RS17800 at 2198.762 kb on - strand, within RPSI07_RS17800 at 2198.780 kb on + strand, within RPSI07_RS17800 at 2198.781 kb on - strand, within RPSI07_RS17800 at 2198.821 kb on + strand, within RPSI07_RS17800 at 2198.821 kb on + strand, within RPSI07_RS17800 at 2198.821 kb on + strand, within RPSI07_RS17800 at 2198.821 kb on + strand, within RPSI07_RS17800 at 2198.821 kb on + strand, within RPSI07_RS17800 at 2198.821 kb on + strand, within RPSI07_RS17800 at 2198.821 kb on + strand, within RPSI07_RS17800 at 2198.822 kb on - strand, within RPSI07_RS17800 at 2198.822 kb on - strand, within RPSI07_RS17800 at 2198.822 kb on - strand, within RPSI07_RS17800 at 2198.822 kb on - strand, within RPSI07_RS17800 at 2198.845 kb on + strand, within RPSI07_RS17800 at 2199.014 kb on - strand, within RPSI07_RS17800 at 2199.014 kb on - strand, within RPSI07_RS17800 at 2199.026 kb on - strand, within RPSI07_RS17800 at 2199.026 kb on - strand, within RPSI07_RS17800 at 2199.026 kb on - strand, within RPSI07_RS17800 at 2199.026 kb on - strand, within RPSI07_RS17800 at 2199.043 kb on + strand, within RPSI07_RS17800 at 2199.270 kb on + strand at 2199.308 kb on + strand at 2199.309 kb on - strand at 2199.586 kb on + strand, within RPSI07_RS17805 at 2199.586 kb on + strand, within RPSI07_RS17805 at 2199.586 kb on + strand, within RPSI07_RS17805 at 2199.587 kb on - strand, within RPSI07_RS17805 at 2199.928 kb on - strand, within RPSI07_RS17805 at 2200.201 kb on + strand, within RPSI07_RS17805 at 2200.611 kb on - strand, within RPSI07_RS17810 at 2201.167 kb on + strand, within RPSI07_RS17815 at 2201.167 kb on + strand, within RPSI07_RS17815
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 2,198,464 + RPSI07_RS17800 0.43 -0.3 2,198,474 - RPSI07_RS17800 0.44 +0.9 2,198,474 - RPSI07_RS17800 0.44 +1.2 2,198,474 - RPSI07_RS17800 0.44 +0.4 2,198,474 - RPSI07_RS17800 0.44 -0.6 2,198,508 - RPSI07_RS17800 0.47 -0.8 2,198,508 - RPSI07_RS17800 0.47 +0.2 2,198,645 - RPSI07_RS17800 0.57 -0.0 2,198,758 + RPSI07_RS17800 0.66 +2.0 2,198,758 + RPSI07_RS17800 0.66 +1.7 2,198,762 - RPSI07_RS17800 0.66 +1.0 2,198,780 + RPSI07_RS17800 0.67 +1.7 2,198,781 - RPSI07_RS17800 0.67 +0.8 2,198,821 + RPSI07_RS17800 0.70 -1.6 2,198,821 + RPSI07_RS17800 0.70 +0.3 2,198,821 + RPSI07_RS17800 0.70 +0.1 2,198,821 + RPSI07_RS17800 0.70 +0.7 2,198,821 + RPSI07_RS17800 0.70 +0.0 2,198,821 + RPSI07_RS17800 0.70 -0.6 2,198,821 + RPSI07_RS17800 0.70 -0.2 2,198,822 - RPSI07_RS17800 0.71 -1.1 2,198,822 - RPSI07_RS17800 0.71 +1.7 2,198,822 - RPSI07_RS17800 0.71 -0.9 2,198,822 - RPSI07_RS17800 0.71 -1.9 2,198,845 + RPSI07_RS17800 0.72 +1.7 2,199,014 - RPSI07_RS17800 0.85 -0.7 2,199,014 - RPSI07_RS17800 0.85 -1.9 2,199,026 - RPSI07_RS17800 0.86 +1.7 2,199,026 - RPSI07_RS17800 0.86 +1.4 2,199,026 - RPSI07_RS17800 0.86 -0.7 2,199,026 - RPSI07_RS17800 0.86 -1.4 2,199,043 + RPSI07_RS17800 0.87 +0.3 2,199,270 + -1.3 2,199,308 + -0.7 2,199,309 - -0.4 2,199,586 + RPSI07_RS17805 0.20 +0.3 2,199,586 + RPSI07_RS17805 0.20 -1.2 2,199,586 + RPSI07_RS17805 0.20 -0.5 2,199,587 - RPSI07_RS17805 0.20 -2.4 2,199,928 - RPSI07_RS17805 0.58 -0.4 2,200,201 + RPSI07_RS17805 0.89 -0.1 2,200,611 - RPSI07_RS17810 0.43 -0.5 2,201,167 + RPSI07_RS17815 0.18 -1.3 2,201,167 + RPSI07_RS17815 0.18 -1.6
Or see this region's nucleotide sequence