Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS11190 and RPSI07_RS11195 are separated by 114 nucleotides RPSI07_RS11195 and RPSI07_RS11200 are separated by 211 nucleotides RPSI07_RS11200 and RPSI07_RS11205 are separated by 61 nucleotides
RPSI07_RS11190: RPSI07_RS11190 - hypothetical protein, at 711,352 to 712,698
_RS11190
RPSI07_RS11195: RPSI07_RS11195 - membrane protein, at 712,813 to 714,360
_RS11195
RPSI07_RS11200: RPSI07_RS11200 - DUF2147 domain-containing protein, at 714,572 to 715,027
_RS11200
RPSI07_RS11205: RPSI07_RS11205 - hemagglutinin, at 715,089 to 716,171
_RS11205
Position (kb) 712
713
714
715 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 711.981 kb on - strand, within RPSI07_RS11190 at 711.996 kb on + strand, within RPSI07_RS11190 at 711.996 kb on + strand, within RPSI07_RS11190 at 711.997 kb on - strand, within RPSI07_RS11190 at 711.997 kb on - strand, within RPSI07_RS11190 at 712.010 kb on + strand, within RPSI07_RS11190 at 712.122 kb on - strand, within RPSI07_RS11190 at 712.122 kb on - strand, within RPSI07_RS11190 at 712.148 kb on + strand, within RPSI07_RS11190 at 712.148 kb on + strand, within RPSI07_RS11190 at 712.149 kb on - strand, within RPSI07_RS11190 at 712.323 kb on - strand, within RPSI07_RS11190 at 712.595 kb on + strand at 712.716 kb on + strand at 712.716 kb on + strand at 712.717 kb on - strand at 712.718 kb on + strand at 712.718 kb on + strand at 712.719 kb on - strand at 712.719 kb on - strand at 712.719 kb on - strand at 712.720 kb on - strand at 712.741 kb on + strand at 712.741 kb on + strand at 712.741 kb on + strand at 712.741 kb on + strand at 712.742 kb on - strand at 712.742 kb on - strand at 712.743 kb on - strand at 712.822 kb on - strand at 713.562 kb on + strand, within RPSI07_RS11195 at 713.563 kb on - strand, within RPSI07_RS11195 at 713.563 kb on - strand, within RPSI07_RS11195 at 713.696 kb on + strand, within RPSI07_RS11195 at 713.697 kb on - strand, within RPSI07_RS11195 at 713.813 kb on + strand, within RPSI07_RS11195 at 713.813 kb on + strand, within RPSI07_RS11195 at 713.813 kb on + strand, within RPSI07_RS11195 at 713.813 kb on + strand, within RPSI07_RS11195 at 713.814 kb on - strand, within RPSI07_RS11195 at 713.814 kb on - strand, within RPSI07_RS11195 at 713.814 kb on - strand, within RPSI07_RS11195 at 713.814 kb on - strand, within RPSI07_RS11195 at 713.885 kb on + strand, within RPSI07_RS11195 at 713.933 kb on + strand, within RPSI07_RS11195 at 713.934 kb on - strand, within RPSI07_RS11195 at 713.984 kb on + strand, within RPSI07_RS11195 at 714.092 kb on + strand, within RPSI07_RS11195 at 714.188 kb on + strand, within RPSI07_RS11195 at 714.188 kb on + strand, within RPSI07_RS11195 at 714.189 kb on - strand, within RPSI07_RS11195 at 714.411 kb on + strand at 714.436 kb on + strand at 714.437 kb on - strand at 714.437 kb on - strand at 714.437 kb on - strand at 714.439 kb on + strand at 714.440 kb on - strand at 714.440 kb on - strand at 714.574 kb on - strand at 714.678 kb on + strand, within RPSI07_RS11200 at 714.679 kb on - strand, within RPSI07_RS11200 at 714.706 kb on + strand, within RPSI07_RS11200 at 714.858 kb on + strand, within RPSI07_RS11200 at 714.858 kb on + strand, within RPSI07_RS11200 at 714.859 kb on - strand, within RPSI07_RS11200 at 714.859 kb on - strand, within RPSI07_RS11200 at 715.106 kb on + strand at 715.106 kb on + strand at 715.275 kb on - strand, within RPSI07_RS11205 at 715.275 kb on - strand, within RPSI07_RS11205
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 711,981 - RPSI07_RS11190 0.47 +1.2 711,996 + RPSI07_RS11190 0.48 +1.0 711,996 + RPSI07_RS11190 0.48 -0.4 711,997 - RPSI07_RS11190 0.48 +0.9 711,997 - RPSI07_RS11190 0.48 -0.9 712,010 + RPSI07_RS11190 0.49 +0.0 712,122 - RPSI07_RS11190 0.57 -0.7 712,122 - RPSI07_RS11190 0.57 +0.0 712,148 + RPSI07_RS11190 0.59 +0.1 712,148 + RPSI07_RS11190 0.59 +0.9 712,149 - RPSI07_RS11190 0.59 -2.5 712,323 - RPSI07_RS11190 0.72 -0.5 712,595 + +0.3 712,716 + -2.8 712,716 + -1.1 712,717 - -0.9 712,718 + -1.0 712,718 + -0.5 712,719 - -0.3 712,719 - -0.6 712,719 - +0.8 712,720 - +3.0 712,741 + -1.5 712,741 + -0.4 712,741 + +0.5 712,741 + +0.6 712,742 - -0.9 712,742 - +1.2 712,743 - +1.3 712,822 - +2.9 713,562 + RPSI07_RS11195 0.48 -0.7 713,563 - RPSI07_RS11195 0.48 +1.1 713,563 - RPSI07_RS11195 0.48 -0.4 713,696 + RPSI07_RS11195 0.57 -0.6 713,697 - RPSI07_RS11195 0.57 -0.6 713,813 + RPSI07_RS11195 0.65 -0.4 713,813 + RPSI07_RS11195 0.65 -1.2 713,813 + RPSI07_RS11195 0.65 -0.5 713,813 + RPSI07_RS11195 0.65 -3.0 713,814 - RPSI07_RS11195 0.65 +0.3 713,814 - RPSI07_RS11195 0.65 +1.9 713,814 - RPSI07_RS11195 0.65 -1.7 713,814 - RPSI07_RS11195 0.65 -0.7 713,885 + RPSI07_RS11195 0.69 +0.3 713,933 + RPSI07_RS11195 0.72 -1.9 713,934 - RPSI07_RS11195 0.72 +4.9 713,984 + RPSI07_RS11195 0.76 +1.4 714,092 + RPSI07_RS11195 0.83 -0.1 714,188 + RPSI07_RS11195 0.89 -2.1 714,188 + RPSI07_RS11195 0.89 -1.0 714,189 - RPSI07_RS11195 0.89 -1.6 714,411 + -1.5 714,436 + +0.8 714,437 - +1.0 714,437 - -1.6 714,437 - +0.3 714,439 + -0.6 714,440 - -0.6 714,440 - +0.2 714,574 - -0.7 714,678 + RPSI07_RS11200 0.23 -0.0 714,679 - RPSI07_RS11200 0.23 -2.9 714,706 + RPSI07_RS11200 0.29 +0.7 714,858 + RPSI07_RS11200 0.63 +2.1 714,858 + RPSI07_RS11200 0.63 -0.5 714,859 - RPSI07_RS11200 0.63 +1.1 714,859 - RPSI07_RS11200 0.63 +0.9 715,106 + +0.9 715,106 + +0.9 715,275 - RPSI07_RS11205 0.17 -2.5 715,275 - RPSI07_RS11205 0.17 +1.4
Or see this region's nucleotide sequence