Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS09255 and RPSI07_RS09260 are separated by 297 nucleotides RPSI07_RS09260 and RPSI07_RS09265 are separated by 177 nucleotides RPSI07_RS09265 and RPSI07_RS09270 are separated by 466 nucleotides RPSI07_RS09270 and RPSI07_RS09275 are separated by 68 nucleotides
RPSI07_RS09255: RPSI07_RS09255 - hypothetical protein, at 300,509 to 300,778
_RS09255
RPSI07_RS09260: RPSI07_RS09260 - transcriptional regulator, at 301,076 to 301,489
_RS09260
RPSI07_RS09265: RPSI07_RS09265 - hypothetical protein, at 301,667 to 302,179
_RS09265
RPSI07_RS09270: RPSI07_RS09270 - hypothetical protein, at 302,646 to 302,915
_RS09270
RPSI07_RS09275: RPSI07_RS09275 - TetR/AcrR family transcriptional regulator, at 302,984 to 303,634
_RS09275
Position (kb) 301
302
303 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 300.726 kb on - strand, within RPSI07_RS09255 at 300.738 kb on + strand, within RPSI07_RS09255 at 300.739 kb on - strand, within RPSI07_RS09255 at 300.773 kb on - strand at 300.821 kb on + strand at 300.821 kb on + strand at 300.821 kb on + strand at 300.821 kb on + strand at 300.821 kb on + strand at 300.821 kb on + strand at 300.821 kb on + strand at 300.821 kb on + strand at 300.821 kb on + strand at 300.821 kb on + strand at 300.821 kb on + strand at 300.821 kb on + strand at 300.821 kb on + strand at 300.822 kb on - strand at 300.822 kb on - strand at 300.822 kb on - strand at 300.822 kb on - strand at 300.822 kb on - strand at 300.822 kb on - strand at 300.822 kb on - strand at 300.822 kb on - strand at 300.822 kb on - strand at 300.822 kb on - strand at 300.822 kb on - strand at 300.822 kb on - strand at 300.822 kb on - strand at 300.822 kb on - strand at 300.822 kb on - strand at 300.822 kb on - strand at 300.823 kb on + strand at 300.823 kb on + strand at 300.823 kb on + strand at 300.824 kb on - strand at 300.824 kb on - strand at 300.829 kb on + strand at 300.885 kb on + strand at 300.885 kb on + strand at 300.886 kb on - strand at 300.886 kb on - strand at 300.886 kb on - strand at 300.886 kb on - strand at 300.886 kb on - strand at 300.975 kb on - strand at 300.975 kb on - strand at 300.975 kb on - strand at 301.015 kb on + strand at 301.015 kb on + strand at 301.015 kb on + strand at 301.016 kb on - strand at 301.016 kb on - strand at 301.016 kb on - strand at 301.016 kb on - strand at 301.016 kb on - strand at 301.016 kb on - strand at 301.016 kb on - strand at 301.016 kb on - strand at 301.016 kb on - strand at 301.077 kb on + strand at 301.077 kb on + strand at 301.077 kb on + strand at 301.077 kb on + strand at 301.077 kb on + strand at 301.077 kb on + strand at 301.077 kb on + strand at 301.078 kb on - strand at 301.078 kb on - strand at 301.078 kb on - strand at 301.078 kb on - strand at 301.078 kb on - strand at 301.078 kb on - strand at 301.078 kb on - strand at 301.078 kb on - strand at 301.078 kb on - strand at 301.078 kb on - strand at 301.078 kb on - strand at 301.155 kb on + strand, within RPSI07_RS09260 at 301.197 kb on + strand, within RPSI07_RS09260 at 301.198 kb on - strand, within RPSI07_RS09260 at 301.198 kb on - strand, within RPSI07_RS09260 at 301.323 kb on + strand, within RPSI07_RS09260 at 301.324 kb on - strand, within RPSI07_RS09260 at 301.433 kb on + strand, within RPSI07_RS09260 at 301.433 kb on + strand, within RPSI07_RS09260 at 301.434 kb on - strand, within RPSI07_RS09260 at 301.494 kb on + strand at 301.494 kb on + strand at 301.494 kb on + strand at 301.495 kb on - strand at 301.495 kb on - strand at 301.508 kb on + strand at 301.508 kb on + strand at 301.508 kb on + strand at 301.509 kb on - strand at 301.540 kb on - strand at 301.821 kb on - strand, within RPSI07_RS09265 at 301.875 kb on - strand, within RPSI07_RS09265 at 301.875 kb on - strand, within RPSI07_RS09265 at 301.875 kb on - strand, within RPSI07_RS09265 at 301.889 kb on + strand, within RPSI07_RS09265 at 301.889 kb on + strand, within RPSI07_RS09265 at 301.890 kb on - strand, within RPSI07_RS09265 at 301.890 kb on - strand, within RPSI07_RS09265 at 301.890 kb on - strand, within RPSI07_RS09265 at 302.076 kb on - strand, within RPSI07_RS09265 at 302.325 kb on + strand at 302.326 kb on - strand at 302.392 kb on + strand at 302.392 kb on + strand at 302.392 kb on + strand at 302.393 kb on - strand at 302.393 kb on - strand at 302.393 kb on - strand at 302.393 kb on - strand at 302.393 kb on - strand at 302.393 kb on - strand at 302.393 kb on - strand at 302.395 kb on + strand at 302.396 kb on - strand at 302.413 kb on + strand at 302.414 kb on - strand at 302.414 kb on - strand at 302.414 kb on - strand at 302.414 kb on - strand at 302.453 kb on + strand at 302.453 kb on + strand at 302.453 kb on + strand at 302.454 kb on - strand at 302.454 kb on - strand at 302.454 kb on - strand at 302.460 kb on + strand at 302.460 kb on + strand at 302.460 kb on - strand at 302.527 kb on + strand at 302.528 kb on - strand at 302.528 kb on - strand at 302.528 kb on - strand at 302.544 kb on + strand at 302.545 kb on - strand at 302.545 kb on - strand at 302.545 kb on - strand at 302.586 kb on + strand at 302.586 kb on + strand at 302.586 kb on + strand at 302.586 kb on + strand at 302.587 kb on - strand at 302.587 kb on - strand at 302.629 kb on + strand at 302.630 kb on - strand at 302.647 kb on + strand at 302.648 kb on - strand at 302.722 kb on + strand, within RPSI07_RS09270 at 302.723 kb on - strand, within RPSI07_RS09270 at 302.723 kb on - strand, within RPSI07_RS09270 at 302.759 kb on + strand, within RPSI07_RS09270 at 302.986 kb on - strand at 303.010 kb on + strand at 303.010 kb on + strand at 303.011 kb on - strand at 303.011 kb on - strand at 303.011 kb on - strand at 303.011 kb on - strand at 303.033 kb on - strand at 303.037 kb on - strand at 303.086 kb on + strand, within RPSI07_RS09275 at 303.086 kb on + strand, within RPSI07_RS09275 at 303.087 kb on - strand, within RPSI07_RS09275 at 303.087 kb on - strand, within RPSI07_RS09275 at 303.145 kb on + strand, within RPSI07_RS09275 at 303.145 kb on + strand, within RPSI07_RS09275 at 303.145 kb on + strand, within RPSI07_RS09275 at 303.146 kb on - strand, within RPSI07_RS09275 at 303.146 kb on - strand, within RPSI07_RS09275 at 303.146 kb on - strand, within RPSI07_RS09275 at 303.146 kb on - strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.147 kb on + strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275 at 303.148 kb on - strand, within RPSI07_RS09275
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 300,726 - RPSI07_RS09255 0.80 +0.5 300,738 + RPSI07_RS09255 0.85 -2.0 300,739 - RPSI07_RS09255 0.85 -0.4 300,773 - +0.3 300,821 + +0.4 300,821 + -0.8 300,821 + -2.4 300,821 + -2.0 300,821 + +0.8 300,821 + +1.2 300,821 + +0.3 300,821 + -1.4 300,821 + +0.8 300,821 + +1.2 300,821 + -1.6 300,821 + +0.2 300,821 + +0.7 300,822 - -0.9 300,822 - +1.8 300,822 - +0.3 300,822 - -0.1 300,822 - -0.6 300,822 - +0.3 300,822 - -0.6 300,822 - +2.6 300,822 - -1.7 300,822 - +1.9 300,822 - -0.5 300,822 - +0.4 300,822 - -0.4 300,822 - -0.2 300,822 - +0.5 300,822 - -0.2 300,823 + +0.8 300,823 + +0.2 300,823 + -1.2 300,824 - -0.4 300,824 - +1.2 300,829 + -0.8 300,885 + -0.5 300,885 + -1.7 300,886 - -0.6 300,886 - +0.8 300,886 - +1.0 300,886 - +2.4 300,886 - -1.5 300,975 - +4.2 300,975 - +0.5 300,975 - +1.1 301,015 + -0.0 301,015 + +1.0 301,015 + +1.8 301,016 - +0.3 301,016 - -0.5 301,016 - +0.5 301,016 - +0.9 301,016 - -1.0 301,016 - -0.7 301,016 - +0.4 301,016 - -0.4 301,016 - +0.9 301,077 + -0.2 301,077 + +1.9 301,077 + +0.8 301,077 + +0.0 301,077 + +4.5 301,077 + +3.4 301,077 + +2.4 301,078 - -0.9 301,078 - -0.4 301,078 - +1.4 301,078 - -0.5 301,078 - +0.9 301,078 - +0.3 301,078 - +1.4 301,078 - -1.9 301,078 - +0.8 301,078 - -1.5 301,078 - -0.8 301,155 + RPSI07_RS09260 0.19 +1.9 301,197 + RPSI07_RS09260 0.29 +0.5 301,198 - RPSI07_RS09260 0.29 +0.3 301,198 - RPSI07_RS09260 0.29 +0.1 301,323 + RPSI07_RS09260 0.60 -1.7 301,324 - RPSI07_RS09260 0.60 -0.8 301,433 + RPSI07_RS09260 0.86 -0.5 301,433 + RPSI07_RS09260 0.86 +0.6 301,434 - RPSI07_RS09260 0.86 +0.8 301,494 + -0.2 301,494 + -0.2 301,494 + +0.4 301,495 - -3.7 301,495 - -0.5 301,508 + -2.0 301,508 + -0.6 301,508 + -1.8 301,509 - -1.1 301,540 - -0.5 301,821 - RPSI07_RS09265 0.30 -0.7 301,875 - RPSI07_RS09265 0.41 -0.2 301,875 - RPSI07_RS09265 0.41 -2.2 301,875 - RPSI07_RS09265 0.41 +0.8 301,889 + RPSI07_RS09265 0.43 +0.8 301,889 + RPSI07_RS09265 0.43 -1.3 301,890 - RPSI07_RS09265 0.43 -2.1 301,890 - RPSI07_RS09265 0.43 +0.4 301,890 - RPSI07_RS09265 0.43 +0.5 302,076 - RPSI07_RS09265 0.80 +0.2 302,325 + +0.9 302,326 - -0.9 302,392 + -1.0 302,392 + +0.1 302,392 + -0.4 302,393 - +0.7 302,393 - +0.1 302,393 - -0.2 302,393 - -2.2 302,393 - -0.5 302,393 - +0.2 302,393 - -1.2 302,395 + -0.3 302,396 - -1.2 302,413 + +1.8 302,414 - +0.1 302,414 - -0.4 302,414 - -0.2 302,414 - -0.1 302,453 + +1.8 302,453 + -0.7 302,453 + +1.6 302,454 - -2.2 302,454 - -0.6 302,454 - +1.2 302,460 + -2.7 302,460 + -1.6 302,460 - +0.8 302,527 + -2.9 302,528 - +0.8 302,528 - +0.6 302,528 - -1.5 302,544 + +1.8 302,545 - -0.2 302,545 - +2.0 302,545 - +0.2 302,586 + +1.8 302,586 + -0.9 302,586 + -0.0 302,586 + -0.4 302,587 - +0.2 302,587 - +0.8 302,629 + +0.3 302,630 - -0.4 302,647 + +0.6 302,648 - -0.8 302,722 + RPSI07_RS09270 0.28 -0.6 302,723 - RPSI07_RS09270 0.29 +1.8 302,723 - RPSI07_RS09270 0.29 -1.5 302,759 + RPSI07_RS09270 0.42 -1.4 302,986 - +0.9 303,010 + -1.9 303,010 + -0.5 303,011 - +4.0 303,011 - -1.5 303,011 - +0.6 303,011 - +1.3 303,033 - -1.3 303,037 - -0.2 303,086 + RPSI07_RS09275 0.16 -0.8 303,086 + RPSI07_RS09275 0.16 +0.3 303,087 - RPSI07_RS09275 0.16 -0.2 303,087 - RPSI07_RS09275 0.16 -1.6 303,145 + RPSI07_RS09275 0.25 +5.3 303,145 + RPSI07_RS09275 0.25 -0.9 303,145 + RPSI07_RS09275 0.25 -1.0 303,146 - RPSI07_RS09275 0.25 +0.1 303,146 - RPSI07_RS09275 0.25 -0.3 303,146 - RPSI07_RS09275 0.25 +1.7 303,146 - RPSI07_RS09275 0.25 -0.4 303,147 + RPSI07_RS09275 0.25 -1.1 303,147 + RPSI07_RS09275 0.25 +3.4 303,147 + RPSI07_RS09275 0.25 -1.1 303,147 + RPSI07_RS09275 0.25 -2.6 303,147 + RPSI07_RS09275 0.25 +0.5 303,147 + RPSI07_RS09275 0.25 -0.7 303,147 + RPSI07_RS09275 0.25 -2.2 303,147 + RPSI07_RS09275 0.25 +0.2 303,147 + RPSI07_RS09275 0.25 +1.2 303,147 + RPSI07_RS09275 0.25 -0.9 303,147 + RPSI07_RS09275 0.25 +1.8 303,147 + RPSI07_RS09275 0.25 -1.2 303,147 + RPSI07_RS09275 0.25 +0.8 303,147 + RPSI07_RS09275 0.25 -0.3 303,147 + RPSI07_RS09275 0.25 +0.0 303,147 + RPSI07_RS09275 0.25 -1.2 303,147 + RPSI07_RS09275 0.25 -1.2 303,147 + RPSI07_RS09275 0.25 -1.8 303,147 + RPSI07_RS09275 0.25 -1.5 303,147 + RPSI07_RS09275 0.25 -0.2 303,147 + RPSI07_RS09275 0.25 +0.1 303,147 + RPSI07_RS09275 0.25 -1.0 303,148 - RPSI07_RS09275 0.25 -0.6 303,148 - RPSI07_RS09275 0.25 +0.8 303,148 - RPSI07_RS09275 0.25 +1.8 303,148 - RPSI07_RS09275 0.25 +1.4 303,148 - RPSI07_RS09275 0.25 -1.5 303,148 - RPSI07_RS09275 0.25 -2.2 303,148 - RPSI07_RS09275 0.25 -3.0 303,148 - RPSI07_RS09275 0.25 +3.8 303,148 - RPSI07_RS09275 0.25 -1.0 303,148 - RPSI07_RS09275 0.25 +0.8 303,148 - RPSI07_RS09275 0.25 -0.8 303,148 - RPSI07_RS09275 0.25 -2.2 303,148 - RPSI07_RS09275 0.25 +0.3 303,148 - RPSI07_RS09275 0.25 -0.4 303,148 - RPSI07_RS09275 0.25 +1.2 303,148 - RPSI07_RS09275 0.25 -0.7 303,148 - RPSI07_RS09275 0.25 -0.5 303,148 - RPSI07_RS09275 0.25 +2.4 303,148 - RPSI07_RS09275 0.25 +2.2 303,148 - RPSI07_RS09275 0.25 +2.8 303,148 - RPSI07_RS09275 0.25 -0.2 303,148 - RPSI07_RS09275 0.25 +1.4 303,148 - RPSI07_RS09275 0.25 -1.4
Or see this region's nucleotide sequence