Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS24220 and RPSI07_RS01790 are separated by 87 nucleotides RPSI07_RS01790 and RPSI07_RS01795 are separated by 123 nucleotides
RPSI07_RS24220: RPSI07_RS24220 - KR domain-containing protein, at 484,285 to 500,325
_RS24220
RPSI07_RS01790: RPSI07_RS01790 - class I SAM-dependent methyltransferase, at 500,413 to 501,279
_RS01790
RPSI07_RS01795: RPSI07_RS01795 - KR domain-containing protein, at 501,403 to 513,870
_RS01795
Position (kb) 500
501
502 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 499.584 kb on + strand at 499.613 kb on - strand at 499.683 kb on + strand at 499.684 kb on - strand at 499.729 kb on + strand at 499.729 kb on + strand at 499.730 kb on - strand at 499.745 kb on - strand at 499.745 kb on - strand at 499.958 kb on - strand at 500.066 kb on + strand at 500.067 kb on - strand at 500.075 kb on - strand at 500.314 kb on + strand at 500.319 kb on - strand at 500.319 kb on - strand at 500.332 kb on + strand at 500.438 kb on + strand at 500.439 kb on - strand at 500.458 kb on + strand at 500.458 kb on + strand at 500.459 kb on - strand at 500.509 kb on + strand, within RPSI07_RS01790 at 500.509 kb on + strand, within RPSI07_RS01790 at 500.509 kb on + strand, within RPSI07_RS01790 at 500.509 kb on + strand, within RPSI07_RS01790 at 500.509 kb on + strand, within RPSI07_RS01790 at 500.509 kb on + strand, within RPSI07_RS01790 at 500.509 kb on + strand, within RPSI07_RS01790 at 500.510 kb on - strand, within RPSI07_RS01790 at 500.510 kb on - strand, within RPSI07_RS01790 at 500.516 kb on - strand, within RPSI07_RS01790 at 500.516 kb on - strand, within RPSI07_RS01790 at 500.516 kb on - strand, within RPSI07_RS01790 at 500.516 kb on - strand, within RPSI07_RS01790 at 500.668 kb on + strand, within RPSI07_RS01790 at 500.668 kb on + strand, within RPSI07_RS01790 at 500.668 kb on + strand, within RPSI07_RS01790 at 500.668 kb on + strand, within RPSI07_RS01790 at 500.669 kb on - strand, within RPSI07_RS01790 at 500.669 kb on - strand, within RPSI07_RS01790 at 500.669 kb on - strand, within RPSI07_RS01790 at 500.669 kb on - strand, within RPSI07_RS01790 at 500.680 kb on + strand, within RPSI07_RS01790 at 500.727 kb on + strand, within RPSI07_RS01790 at 500.728 kb on - strand, within RPSI07_RS01790 at 500.918 kb on - strand, within RPSI07_RS01790 at 500.943 kb on + strand, within RPSI07_RS01790 at 501.057 kb on + strand, within RPSI07_RS01790 at 501.085 kb on - strand, within RPSI07_RS01790 at 501.150 kb on + strand, within RPSI07_RS01790 at 501.150 kb on + strand, within RPSI07_RS01790 at 501.151 kb on - strand, within RPSI07_RS01790 at 501.151 kb on - strand, within RPSI07_RS01790 at 501.151 kb on - strand, within RPSI07_RS01790 at 501.172 kb on + strand, within RPSI07_RS01790 at 501.173 kb on - strand, within RPSI07_RS01790 at 501.247 kb on + strand at 501.247 kb on + strand at 501.247 kb on + strand at 501.248 kb on - strand at 501.248 kb on - strand at 501.277 kb on + strand at 501.373 kb on + strand at 501.374 kb on + strand at 501.374 kb on + strand at 501.374 kb on + strand at 501.374 kb on + strand at 501.374 kb on + strand at 501.374 kb on + strand at 501.374 kb on + strand at 501.374 kb on + strand at 501.374 kb on + strand at 501.374 kb on + strand at 501.374 kb on + strand at 501.374 kb on + strand at 501.374 kb on + strand at 501.375 kb on - strand at 501.375 kb on - strand at 501.375 kb on - strand at 501.375 kb on - strand at 501.375 kb on - strand at 501.375 kb on - strand at 501.375 kb on - strand at 501.769 kb on + strand at 501.770 kb on - strand at 501.790 kb on + strand at 501.790 kb on + strand at 501.790 kb on + strand at 501.790 kb on + strand at 501.791 kb on - strand at 501.820 kb on + strand at 501.820 kb on + strand at 501.821 kb on - strand at 501.860 kb on + strand at 501.879 kb on + strand at 501.879 kb on + strand at 501.879 kb on + strand at 501.880 kb on - strand at 501.964 kb on + strand at 501.964 kb on + strand at 501.964 kb on + strand at 501.965 kb on - strand at 501.965 kb on - strand at 502.028 kb on - strand at 502.084 kb on + strand at 502.084 kb on + strand at 502.084 kb on + strand at 502.085 kb on - strand at 502.085 kb on - strand at 502.122 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 499,584 + +0.1 499,613 - +0.8 499,683 + -1.3 499,684 - -0.2 499,729 + -1.0 499,729 + +1.4 499,730 - +0.6 499,745 - -0.0 499,745 - -0.9 499,958 - +2.2 500,066 + -0.1 500,067 - +0.2 500,075 - -2.0 500,314 + +2.8 500,319 - -0.6 500,319 - -0.6 500,332 + +0.1 500,438 + -2.2 500,439 - -0.8 500,458 + -0.2 500,458 + +1.9 500,459 - -0.6 500,509 + RPSI07_RS01790 0.11 +0.4 500,509 + RPSI07_RS01790 0.11 +0.5 500,509 + RPSI07_RS01790 0.11 +0.6 500,509 + RPSI07_RS01790 0.11 -0.4 500,509 + RPSI07_RS01790 0.11 -1.1 500,509 + RPSI07_RS01790 0.11 -0.2 500,509 + RPSI07_RS01790 0.11 +1.6 500,510 - RPSI07_RS01790 0.11 +1.3 500,510 - RPSI07_RS01790 0.11 -1.7 500,516 - RPSI07_RS01790 0.12 -2.3 500,516 - RPSI07_RS01790 0.12 +0.8 500,516 - RPSI07_RS01790 0.12 -0.9 500,516 - RPSI07_RS01790 0.12 -1.1 500,668 + RPSI07_RS01790 0.29 +1.0 500,668 + RPSI07_RS01790 0.29 -0.9 500,668 + RPSI07_RS01790 0.29 +0.5 500,668 + RPSI07_RS01790 0.29 -0.8 500,669 - RPSI07_RS01790 0.30 +0.5 500,669 - RPSI07_RS01790 0.30 -0.4 500,669 - RPSI07_RS01790 0.30 -0.4 500,669 - RPSI07_RS01790 0.30 +0.2 500,680 + RPSI07_RS01790 0.31 -1.3 500,727 + RPSI07_RS01790 0.36 -2.1 500,728 - RPSI07_RS01790 0.36 +2.2 500,918 - RPSI07_RS01790 0.58 -0.4 500,943 + RPSI07_RS01790 0.61 -2.0 501,057 + RPSI07_RS01790 0.74 +0.5 501,085 - RPSI07_RS01790 0.78 +1.0 501,150 + RPSI07_RS01790 0.85 +2.8 501,150 + RPSI07_RS01790 0.85 -2.0 501,151 - RPSI07_RS01790 0.85 +0.1 501,151 - RPSI07_RS01790 0.85 -2.5 501,151 - RPSI07_RS01790 0.85 -1.3 501,172 + RPSI07_RS01790 0.88 +1.9 501,173 - RPSI07_RS01790 0.88 +0.1 501,247 + +0.6 501,247 + +0.0 501,247 + +0.6 501,248 - +3.3 501,248 - +1.0 501,277 + -0.9 501,373 + -2.9 501,374 + +2.4 501,374 + +0.5 501,374 + -0.5 501,374 + -0.2 501,374 + +0.3 501,374 + -2.0 501,374 + +0.1 501,374 + -0.3 501,374 + -0.6 501,374 + +0.2 501,374 + +0.7 501,374 + -0.5 501,374 + -1.5 501,375 - +0.6 501,375 - -0.0 501,375 - -0.7 501,375 - -4.5 501,375 - +0.6 501,375 - +0.2 501,375 - -1.1 501,769 + -1.9 501,770 - -2.1 501,790 + +3.4 501,790 + -1.7 501,790 + -0.4 501,790 + +0.0 501,791 - +1.1 501,820 + +0.4 501,820 + -0.3 501,821 - -0.8 501,860 + +0.8 501,879 + +0.0 501,879 + -0.2 501,879 + -0.0 501,880 - -0.8 501,964 + +0.6 501,964 + +1.2 501,964 + +1.8 501,965 - -2.4 501,965 - +0.7 502,028 - -1.0 502,084 + -0.7 502,084 + +1.5 502,084 + +0.7 502,085 - -1.3 502,085 - +0.1 502,122 + -0.9
Or see this region's nucleotide sequence