Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS00990 and RPSI07_RS00995 are separated by 119 nucleotides RPSI07_RS00995 and RPSI07_RS01000 are separated by 109 nucleotides RPSI07_RS01000 and RPSI07_RS01005 are separated by 79 nucleotides RPSI07_RS01005 and RPSI07_RS01010 are separated by 445 nucleotides
RPSI07_RS00990: RPSI07_RS00990 - siderophore-interacting protein, at 273,217 to 274,050
_RS00990
RPSI07_RS00995: RPSI07_RS00995 - PadR family transcriptional regulator, at 274,170 to 274,826
_RS00995
RPSI07_RS01000: RPSI07_RS01000 - pyroglutamyl-peptidase I, at 274,936 to 275,583
_RS01000
RPSI07_RS01005: RPSI07_RS01005 - MarR family transcriptional regulator, at 275,663 to 276,082
_RS01005
RPSI07_RS01010: RPSI07_RS01010 - DUF3757 domain-containing protein, at 276,528 to 276,908
_RS01010
Position (kb) 274
275
276 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 274.050 kb on + strand at 274.051 kb on - strand at 274.051 kb on - strand at 274.051 kb on - strand at 274.051 kb on - strand at 274.166 kb on + strand at 274.166 kb on + strand at 274.167 kb on - strand at 274.167 kb on - strand at 274.167 kb on - strand at 274.167 kb on - strand at 274.462 kb on + strand, within RPSI07_RS00995 at 274.462 kb on + strand, within RPSI07_RS00995 at 274.516 kb on + strand, within RPSI07_RS00995 at 274.517 kb on - strand, within RPSI07_RS00995 at 274.532 kb on - strand, within RPSI07_RS00995 at 274.556 kb on - strand, within RPSI07_RS00995 at 274.841 kb on + strand at 274.841 kb on + strand at 274.842 kb on - strand at 274.934 kb on - strand at 275.141 kb on + strand, within RPSI07_RS01000 at 275.162 kb on + strand, within RPSI07_RS01000 at 275.162 kb on + strand, within RPSI07_RS01000 at 275.162 kb on + strand, within RPSI07_RS01000 at 275.162 kb on + strand, within RPSI07_RS01000 at 275.162 kb on + strand, within RPSI07_RS01000 at 275.162 kb on + strand, within RPSI07_RS01000 at 275.162 kb on + strand, within RPSI07_RS01000 at 275.162 kb on + strand, within RPSI07_RS01000 at 275.162 kb on + strand, within RPSI07_RS01000 at 275.162 kb on + strand, within RPSI07_RS01000 at 275.163 kb on - strand, within RPSI07_RS01000 at 275.163 kb on - strand, within RPSI07_RS01000 at 275.163 kb on - strand, within RPSI07_RS01000 at 275.163 kb on - strand, within RPSI07_RS01000 at 275.166 kb on + strand, within RPSI07_RS01000 at 275.167 kb on - strand, within RPSI07_RS01000 at 275.249 kb on + strand, within RPSI07_RS01000 at 275.249 kb on + strand, within RPSI07_RS01000 at 275.250 kb on - strand, within RPSI07_RS01000 at 275.269 kb on + strand, within RPSI07_RS01000 at 275.270 kb on - strand, within RPSI07_RS01000 at 275.572 kb on + strand at 275.572 kb on + strand at 275.572 kb on + strand at 275.572 kb on + strand at 275.573 kb on - strand at 275.573 kb on - strand at 275.573 kb on - strand at 275.573 kb on - strand at 275.635 kb on - strand at 275.635 kb on - strand at 275.635 kb on - strand at 276.009 kb on + strand, within RPSI07_RS01005 at 276.010 kb on - strand, within RPSI07_RS01005 at 276.010 kb on - strand, within RPSI07_RS01005 at 276.010 kb on - strand, within RPSI07_RS01005 at 276.063 kb on + strand at 276.064 kb on - strand at 276.064 kb on - strand at 276.361 kb on + strand at 276.425 kb on - strand at 276.545 kb on + strand at 276.552 kb on + strand at 276.553 kb on - strand at 276.553 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Polymyxin_and_Chloramphenicol remove 274,050 + +2.6 274,051 - +0.6 274,051 - +0.1 274,051 - -0.1 274,051 - -0.4 274,166 + +2.2 274,166 + +1.2 274,167 - -0.5 274,167 - -0.0 274,167 - +1.9 274,167 - +1.8 274,462 + RPSI07_RS00995 0.44 +2.2 274,462 + RPSI07_RS00995 0.44 +2.9 274,516 + RPSI07_RS00995 0.53 +0.6 274,517 - RPSI07_RS00995 0.53 -1.4 274,532 - RPSI07_RS00995 0.55 -1.0 274,556 - RPSI07_RS00995 0.59 -2.0 274,841 + +0.6 274,841 + -1.7 274,842 - -2.0 274,934 - +3.8 275,141 + RPSI07_RS01000 0.32 +0.4 275,162 + RPSI07_RS01000 0.35 +1.5 275,162 + RPSI07_RS01000 0.35 -1.3 275,162 + RPSI07_RS01000 0.35 -0.5 275,162 + RPSI07_RS01000 0.35 +1.2 275,162 + RPSI07_RS01000 0.35 +1.3 275,162 + RPSI07_RS01000 0.35 -0.3 275,162 + RPSI07_RS01000 0.35 +0.0 275,162 + RPSI07_RS01000 0.35 -0.1 275,162 + RPSI07_RS01000 0.35 +1.4 275,162 + RPSI07_RS01000 0.35 -1.0 275,163 - RPSI07_RS01000 0.35 +1.7 275,163 - RPSI07_RS01000 0.35 -0.1 275,163 - RPSI07_RS01000 0.35 -0.2 275,163 - RPSI07_RS01000 0.35 +0.8 275,166 + RPSI07_RS01000 0.35 -0.4 275,167 - RPSI07_RS01000 0.36 +0.6 275,249 + RPSI07_RS01000 0.48 -1.4 275,249 + RPSI07_RS01000 0.48 -0.4 275,250 - RPSI07_RS01000 0.48 +0.3 275,269 + RPSI07_RS01000 0.51 +1.6 275,270 - RPSI07_RS01000 0.52 -0.6 275,572 + +1.2 275,572 + -0.8 275,572 + -0.2 275,572 + +0.6 275,573 - -1.1 275,573 - -0.5 275,573 - -0.1 275,573 - -0.5 275,635 - +0.9 275,635 - +3.8 275,635 - +1.0 276,009 + RPSI07_RS01005 0.82 -0.1 276,010 - RPSI07_RS01005 0.83 +0.2 276,010 - RPSI07_RS01005 0.83 -2.2 276,010 - RPSI07_RS01005 0.83 +0.2 276,063 + -1.1 276,064 - +0.3 276,064 - +0.4 276,361 + -0.5 276,425 - -0.3 276,545 + +2.3 276,552 + +1.0 276,553 - -1.2 276,553 - +1.6
Or see this region's nucleotide sequence