Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS23745 and RPSI07_RS23750 are separated by 197 nucleotides RPSI07_RS23750 and RPSI07_RS23755 are separated by 47 nucleotides
RPSI07_RS23745: RPSI07_RS23745 - hypothetical protein, at 3,472,375 to 3,473,667
_RS23745
RPSI07_RS23750: RPSI07_RS23750 - DNA-binding response regulator, at 3,473,865 to 3,474,443
_RS23750
RPSI07_RS23755: RPSI07_RS23755 - sensor histidine kinase, at 3,474,491 to 3,475,837
_RS23755
Position (kb)
3473
3474
3475 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 3472.953 kb on + strand, within RPSI07_RS23745 at 3472.955 kb on + strand, within RPSI07_RS23745 at 3472.955 kb on + strand, within RPSI07_RS23745 at 3472.955 kb on + strand, within RPSI07_RS23745 at 3472.955 kb on + strand, within RPSI07_RS23745 at 3472.956 kb on - strand, within RPSI07_RS23745 at 3472.956 kb on - strand, within RPSI07_RS23745 at 3472.956 kb on - strand, within RPSI07_RS23745 at 3473.054 kb on + strand, within RPSI07_RS23745 at 3473.054 kb on + strand, within RPSI07_RS23745 at 3473.055 kb on - strand, within RPSI07_RS23745 at 3473.108 kb on + strand, within RPSI07_RS23745 at 3473.108 kb on + strand, within RPSI07_RS23745 at 3473.108 kb on + strand, within RPSI07_RS23745 at 3473.108 kb on + strand at 3473.109 kb on - strand, within RPSI07_RS23745 at 3473.254 kb on + strand, within RPSI07_RS23745 at 3473.255 kb on - strand, within RPSI07_RS23745 at 3473.255 kb on - strand, within RPSI07_RS23745 at 3473.255 kb on - strand, within RPSI07_RS23745 at 3473.462 kb on + strand, within RPSI07_RS23745 at 3473.487 kb on + strand, within RPSI07_RS23745 at 3473.487 kb on + strand, within RPSI07_RS23745 at 3473.488 kb on - strand, within RPSI07_RS23745 at 3473.488 kb on - strand, within RPSI07_RS23745 at 3473.488 kb on - strand, within RPSI07_RS23745 at 3473.605 kb on + strand at 3473.659 kb on - strand at 3473.659 kb on - strand at 3473.659 kb on - strand at 3473.659 kb on - strand at 3473.671 kb on + strand at 3473.671 kb on + strand at 3473.671 kb on + strand at 3473.672 kb on - strand at 3473.672 kb on - strand at 3473.672 kb on - strand at 3473.672 kb on - strand at 3473.672 kb on - strand at 3473.672 kb on - strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.677 kb on + strand at 3473.678 kb on - strand at 3473.678 kb on - strand at 3473.678 kb on - strand at 3473.683 kb on + strand at 3473.683 kb on + strand at 3473.683 kb on + strand at 3473.768 kb on + strand at 3473.768 kb on + strand at 3473.768 kb on + strand at 3473.768 kb on + strand at 3473.768 kb on + strand at 3473.769 kb on - strand at 3473.769 kb on - strand at 3473.769 kb on - strand at 3473.769 kb on - strand at 3473.769 kb on - strand at 3473.866 kb on + strand at 3473.866 kb on + strand at 3473.866 kb on + strand at 3473.867 kb on - strand at 3473.867 kb on - strand at 3473.867 kb on - strand at 3474.106 kb on + strand, within RPSI07_RS23750 at 3474.106 kb on + strand, within RPSI07_RS23750 at 3474.106 kb on + strand, within RPSI07_RS23750 at 3474.106 kb on + strand, within RPSI07_RS23750 at 3474.107 kb on - strand, within RPSI07_RS23750 at 3474.107 kb on - strand, within RPSI07_RS23750 at 3474.293 kb on - strand, within RPSI07_RS23750 at 3474.293 kb on - strand, within RPSI07_RS23750 at 3474.496 kb on + strand at 3474.496 kb on + strand at 3474.497 kb on - strand at 3474.497 kb on - strand at 3474.541 kb on + strand at 3474.541 kb on + strand at 3474.542 kb on - strand at 3474.542 kb on - strand at 3474.542 kb on - strand at 3474.611 kb on - strand at 3474.645 kb on + strand, within RPSI07_RS23755 at 3474.645 kb on + strand, within RPSI07_RS23755 at 3474.646 kb on - strand, within RPSI07_RS23755 at 3474.646 kb on - strand, within RPSI07_RS23755 at 3474.689 kb on - strand, within RPSI07_RS23755 at 3475.368 kb on + strand, within RPSI07_RS23755 at 3475.369 kb on - strand, within RPSI07_RS23755 at 3475.369 kb on - strand, within RPSI07_RS23755 at 3475.370 kb on - strand, within RPSI07_RS23755 at 3475.410 kb on + strand, within RPSI07_RS23755 at 3475.410 kb on + strand, within RPSI07_RS23755 at 3475.410 kb on + strand, within RPSI07_RS23755 at 3475.410 kb on + strand, within RPSI07_RS23755 at 3475.411 kb on - strand, within RPSI07_RS23755 at 3475.411 kb on - strand, within RPSI07_RS23755 at 3475.411 kb on - strand, within RPSI07_RS23755 at 3475.411 kb on - strand, within RPSI07_RS23755 at 3475.411 kb on - strand, within RPSI07_RS23755 at 3475.411 kb on - strand, within RPSI07_RS23755 at 3475.411 kb on - strand, within RPSI07_RS23755 at 3475.431 kb on + strand, within RPSI07_RS23755
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 3,472,953 + RPSI07_RS23745 0.45 +2.1 3,472,955 + RPSI07_RS23745 0.45 -1.3 3,472,955 + RPSI07_RS23745 0.45 +0.4 3,472,955 + RPSI07_RS23745 0.45 -0.5 3,472,955 + RPSI07_RS23745 0.45 +0.8 3,472,956 - RPSI07_RS23745 0.45 +0.1 3,472,956 - RPSI07_RS23745 0.45 +0.3 3,472,956 - RPSI07_RS23745 0.45 +1.3 3,473,054 + RPSI07_RS23745 0.53 +0.3 3,473,054 + RPSI07_RS23745 0.53 +1.6 3,473,055 - RPSI07_RS23745 0.53 -1.4 3,473,108 + RPSI07_RS23745 0.57 +3.5 3,473,108 + RPSI07_RS23745 0.57 +1.9 3,473,108 + RPSI07_RS23745 0.57 +0.2 3,473,108 + +1.5 3,473,109 - RPSI07_RS23745 0.57 +2.3 3,473,254 + RPSI07_RS23745 0.68 +1.2 3,473,255 - RPSI07_RS23745 0.68 +2.1 3,473,255 - RPSI07_RS23745 0.68 -1.6 3,473,255 - RPSI07_RS23745 0.68 -0.9 3,473,462 + RPSI07_RS23745 0.84 +0.5 3,473,487 + RPSI07_RS23745 0.86 +0.7 3,473,487 + RPSI07_RS23745 0.86 -0.3 3,473,488 - RPSI07_RS23745 0.86 +0.9 3,473,488 - RPSI07_RS23745 0.86 -1.8 3,473,488 - RPSI07_RS23745 0.86 +2.4 3,473,605 + +1.3 3,473,659 - -3.2 3,473,659 - -1.3 3,473,659 - +1.5 3,473,659 - +0.2 3,473,671 + +1.5 3,473,671 + -1.1 3,473,671 + -3.8 3,473,672 - -0.1 3,473,672 - -0.9 3,473,672 - -0.7 3,473,672 - +4.5 3,473,672 - -0.1 3,473,672 - -1.9 3,473,677 + +0.0 3,473,677 + +0.1 3,473,677 + +2.1 3,473,677 + +0.5 3,473,677 + -0.5 3,473,677 + -0.5 3,473,677 + +0.4 3,473,677 + -3.3 3,473,677 + -3.1 3,473,677 + -1.1 3,473,678 - -0.5 3,473,678 - +2.1 3,473,678 - +3.8 3,473,683 + -0.1 3,473,683 + -0.4 3,473,683 + +0.2 3,473,768 + -0.9 3,473,768 + +2.1 3,473,768 + +1.1 3,473,768 + -0.2 3,473,768 + -0.4 3,473,769 - -1.6 3,473,769 - -2.7 3,473,769 - +0.5 3,473,769 - -0.5 3,473,769 - -0.6 3,473,866 + +1.5 3,473,866 + +0.5 3,473,866 + -0.7 3,473,867 - -0.4 3,473,867 - -1.4 3,473,867 - -0.5 3,474,106 + RPSI07_RS23750 0.42 +1.5 3,474,106 + RPSI07_RS23750 0.42 -2.5 3,474,106 + RPSI07_RS23750 0.42 +0.5 3,474,106 + RPSI07_RS23750 0.42 -0.2 3,474,107 - RPSI07_RS23750 0.42 -3.2 3,474,107 - RPSI07_RS23750 0.42 -1.2 3,474,293 - RPSI07_RS23750 0.74 -1.6 3,474,293 - RPSI07_RS23750 0.74 -1.8 3,474,496 + -2.5 3,474,496 + -1.8 3,474,497 - +1.5 3,474,497 - -0.2 3,474,541 + -1.5 3,474,541 + +1.5 3,474,542 - +1.5 3,474,542 - -0.7 3,474,542 - -0.0 3,474,611 - +1.5 3,474,645 + RPSI07_RS23755 0.11 -2.0 3,474,645 + RPSI07_RS23755 0.11 -2.2 3,474,646 - RPSI07_RS23755 0.12 -0.2 3,474,646 - RPSI07_RS23755 0.12 +1.5 3,474,689 - RPSI07_RS23755 0.15 -0.1 3,475,368 + RPSI07_RS23755 0.65 -1.8 3,475,369 - RPSI07_RS23755 0.65 -1.1 3,475,369 - RPSI07_RS23755 0.65 -1.4 3,475,370 - RPSI07_RS23755 0.65 -0.4 3,475,410 + RPSI07_RS23755 0.68 -1.9 3,475,410 + RPSI07_RS23755 0.68 -2.9 3,475,410 + RPSI07_RS23755 0.68 -0.1 3,475,410 + RPSI07_RS23755 0.68 -2.5 3,475,411 - RPSI07_RS23755 0.68 -0.0 3,475,411 - RPSI07_RS23755 0.68 +0.7 3,475,411 - RPSI07_RS23755 0.68 -0.5 3,475,411 - RPSI07_RS23755 0.68 -0.7 3,475,411 - RPSI07_RS23755 0.68 -1.2 3,475,411 - RPSI07_RS23755 0.68 -0.6 3,475,411 - RPSI07_RS23755 0.68 +0.4 3,475,431 + RPSI07_RS23755 0.70 -2.2
Or see this region's nucleotide sequence