Strain Fitness in Ralstonia solanacearum PSI07 around RPSI07_RS22445

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRPSI07_RS22435 and RPSI07_RS22440 are separated by 40 nucleotidesRPSI07_RS22440 and RPSI07_RS22445 overlap by 32 nucleotidesRPSI07_RS22445 and RPSI07_RS22450 are separated by 108 nucleotides RPSI07_RS22435: RPSI07_RS22435 - phosphoenolpyruvate--protein phosphotransferase, at 3,189,694 to 3,191,454 _RS22435 RPSI07_RS22440: RPSI07_RS22440 - HPr family phosphocarrier protein, at 3,191,495 to 3,191,764 _RS22440 RPSI07_RS22445: RPSI07_RS22445 - PTS fructose transporter subunit IIA, at 3,191,733 to 3,192,188 _RS22445 RPSI07_RS22450: RPSI07_RS22450 - glutathione synthase, at 3,192,297 to 3,193,271 _RS22450 Position (kb) 3191 3192 3193Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3 4at 3191.081 kb on + strand, within RPSI07_RS22435at 3191.153 kb on + strand, within RPSI07_RS22435at 3191.361 kb on - strandat 3191.361 kb on - strandat 3191.361 kb on - strandat 3191.394 kb on + strandat 3191.395 kb on - strandat 3191.395 kb on - strandat 3191.445 kb on + strandat 3191.446 kb on - strandat 3191.446 kb on - strandat 3191.465 kb on + strandat 3191.466 kb on - strandat 3191.496 kb on + strandat 3191.734 kb on + strandat 3191.734 kb on + strandat 3191.734 kb on + strandat 3191.735 kb on - strandat 3191.749 kb on + strandat 3191.750 kb on - strandat 3191.750 kb on - strandat 3191.750 kb on - strandat 3191.764 kb on + strandat 3191.764 kb on + strandat 3191.764 kb on + strandat 3191.764 kb on + strandat 3191.764 kb on + strandat 3191.765 kb on - strandat 3191.765 kb on - strandat 3191.765 kb on - strandat 3191.765 kb on - strandat 3191.765 kb on - strandat 3191.820 kb on + strand, within RPSI07_RS22445at 3192.118 kb on + strand, within RPSI07_RS22445at 3192.118 kb on + strand, within RPSI07_RS22445at 3192.118 kb on + strand, within RPSI07_RS22445at 3192.221 kb on + strandat 3192.221 kb on + strandat 3192.221 kb on + strandat 3192.221 kb on + strandat 3192.222 kb on - strandat 3192.222 kb on - strandat 3192.222 kb on - strandat 3192.273 kb on + strandat 3192.299 kb on - strandat 3192.299 kb on - strandat 3192.442 kb on + strand, within RPSI07_RS22450at 3192.442 kb on + strand, within RPSI07_RS22450at 3192.597 kb on + strand, within RPSI07_RS22450at 3192.598 kb on - strand, within RPSI07_RS22450at 3192.676 kb on + strand, within RPSI07_RS22450at 3192.676 kb on + strand, within RPSI07_RS22450at 3192.676 kb on + strand, within RPSI07_RS22450at 3192.677 kb on - strand, within RPSI07_RS22450at 3192.677 kb on - strand, within RPSI07_RS22450at 3192.677 kb on - strand, within RPSI07_RS22450at 3192.991 kb on + strand, within RPSI07_RS22450at 3192.992 kb on - strand, within RPSI07_RS22450at 3192.992 kb on - strand, within RPSI07_RS22450at 3192.992 kb on - strand, within RPSI07_RS22450at 3193.166 kb on + strand, within RPSI07_RS22450at 3193.166 kb on + strand, within RPSI07_RS22450at 3193.168 kb on + strand, within RPSI07_RS22450at 3193.168 kb on + strand, within RPSI07_RS22450at 3193.168 kb on + strand, within RPSI07_RS22450at 3193.168 kb on + strand, within RPSI07_RS22450at 3193.169 kb on - strand, within RPSI07_RS22450at 3193.169 kb on - strand, within RPSI07_RS22450at 3193.169 kb on - strand, within RPSI07_RS22450at 3193.169 kb on - strand, within RPSI07_RS22450

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
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3,191,081 + RPSI07_RS22435 0.79 -4.2
3,191,153 + RPSI07_RS22435 0.83 -0.5
3,191,361 - -1.7
3,191,361 - -3.7
3,191,361 - -1.3
3,191,394 + -2.3
3,191,395 - -2.4
3,191,395 - -1.5
3,191,445 + -2.2
3,191,446 - -1.7
3,191,446 - -2.5
3,191,465 + -4.8
3,191,466 - -1.5
3,191,496 + -1.9
3,191,734 + +1.9
3,191,734 + -0.4
3,191,734 + -3.2
3,191,735 - -1.0
3,191,749 + -1.8
3,191,750 - -1.6
3,191,750 - +2.0
3,191,750 - -2.1
3,191,764 + -4.1
3,191,764 + -3.1
3,191,764 + -1.8
3,191,764 + -1.4
3,191,764 + -2.8
3,191,765 - -0.7
3,191,765 - +0.3
3,191,765 - -0.6
3,191,765 - -1.8
3,191,765 - +0.7
3,191,820 + RPSI07_RS22445 0.19 +4.3
3,192,118 + RPSI07_RS22445 0.84 +0.8
3,192,118 + RPSI07_RS22445 0.84 +3.8
3,192,118 + RPSI07_RS22445 0.84 +1.1
3,192,221 + -0.2
3,192,221 + -1.1
3,192,221 + -0.7
3,192,221 + -1.1
3,192,222 - +0.1
3,192,222 - -0.1
3,192,222 - -1.6
3,192,273 + -0.1
3,192,299 - +0.3
3,192,299 - -0.6
3,192,442 + RPSI07_RS22450 0.15 -0.5
3,192,442 + RPSI07_RS22450 0.15 +2.5
3,192,597 + RPSI07_RS22450 0.31 +1.5
3,192,598 - RPSI07_RS22450 0.31 +0.5
3,192,676 + RPSI07_RS22450 0.39 -0.5
3,192,676 + RPSI07_RS22450 0.39 -1.5
3,192,676 + RPSI07_RS22450 0.39 -0.5
3,192,677 - RPSI07_RS22450 0.39 -2.0
3,192,677 - RPSI07_RS22450 0.39 +1.5
3,192,677 - RPSI07_RS22450 0.39 -1.1
3,192,991 + RPSI07_RS22450 0.71 -0.8
3,192,992 - RPSI07_RS22450 0.71 -0.8
3,192,992 - RPSI07_RS22450 0.71 -0.4
3,192,992 - RPSI07_RS22450 0.71 -1.8
3,193,166 + RPSI07_RS22450 0.89 +0.5
3,193,166 + RPSI07_RS22450 0.89 +1.5
3,193,168 + RPSI07_RS22450 0.89 +0.5
3,193,168 + RPSI07_RS22450 0.89 +2.5
3,193,168 + RPSI07_RS22450 0.89 +0.5
3,193,168 + RPSI07_RS22450 0.89 +1.5
3,193,169 - RPSI07_RS22450 0.89 -1.1
3,193,169 - RPSI07_RS22450 0.89 -2.0
3,193,169 - RPSI07_RS22450 0.89 +0.9
3,193,169 - RPSI07_RS22450 0.89 +0.5

Or see this region's nucleotide sequence