Experiment: SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RPSI07_RS21040 and RPSI07_RS21045 are separated by 312 nucleotides RPSI07_RS21045 and RPSI07_RS21050 are separated by 55 nucleotides RPSI07_RS21050 and RPSI07_RS21055 are separated by 73 nucleotides
RPSI07_RS21040: RPSI07_RS21040 - HD-GYP domain-containing protein, at 2,885,712 to 2,886,923
_RS21040
RPSI07_RS21045: RPSI07_RS21045 - hypothetical protein, at 2,887,236 to 2,887,586
_RS21045
RPSI07_RS21050: RPSI07_RS21050 - MarR family transcriptional regulator, at 2,887,642 to 2,888,154
_RS21050
RPSI07_RS21055: RPSI07_RS21055 - branched-chain amino acid ABC transporter substrate-binding protein, at 2,888,228 to 2,889,385
_RS21055
Position (kb)
2887
2888
2889 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2886.657 kb on - strand, within RPSI07_RS21040 at 2886.923 kb on + strand at 2886.924 kb on - strand at 2886.924 kb on - strand at 2886.928 kb on + strand at 2886.929 kb on - strand at 2886.929 kb on - strand at 2887.216 kb on + strand at 2887.217 kb on - strand at 2887.244 kb on + strand at 2887.245 kb on - strand at 2887.245 kb on - strand at 2887.245 kb on - strand at 2887.268 kb on - strand at 2887.268 kb on - strand at 2887.432 kb on - strand, within RPSI07_RS21045 at 2887.432 kb on - strand, within RPSI07_RS21045 at 2887.519 kb on - strand, within RPSI07_RS21045 at 2887.519 kb on - strand, within RPSI07_RS21045 at 2887.633 kb on + strand at 2887.633 kb on + strand at 2887.634 kb on - strand at 2887.636 kb on + strand at 2887.636 kb on + strand at 2887.636 kb on + strand at 2887.637 kb on - strand at 2887.637 kb on - strand at 2887.637 kb on - strand at 2887.637 kb on - strand at 2887.637 kb on - strand at 2887.860 kb on + strand, within RPSI07_RS21050 at 2887.860 kb on + strand, within RPSI07_RS21050 at 2888.012 kb on + strand, within RPSI07_RS21050 at 2888.012 kb on + strand, within RPSI07_RS21050 at 2888.012 kb on + strand, within RPSI07_RS21050 at 2888.012 kb on + strand, within RPSI07_RS21050 at 2888.012 kb on + strand, within RPSI07_RS21050 at 2888.013 kb on - strand, within RPSI07_RS21050 at 2888.013 kb on - strand, within RPSI07_RS21050 at 2888.016 kb on + strand, within RPSI07_RS21050 at 2888.016 kb on + strand, within RPSI07_RS21050 at 2888.016 kb on + strand, within RPSI07_RS21050 at 2888.016 kb on + strand, within RPSI07_RS21050 at 2888.016 kb on + strand, within RPSI07_RS21050 at 2888.016 kb on + strand, within RPSI07_RS21050 at 2888.017 kb on - strand, within RPSI07_RS21050 at 2888.017 kb on - strand, within RPSI07_RS21050 at 2888.017 kb on - strand, within RPSI07_RS21050 at 2888.017 kb on - strand, within RPSI07_RS21050 at 2888.017 kb on - strand, within RPSI07_RS21050 at 2888.069 kb on + strand, within RPSI07_RS21050 at 2888.070 kb on - strand, within RPSI07_RS21050 at 2888.185 kb on + strand at 2888.185 kb on + strand at 2888.185 kb on + strand at 2888.186 kb on - strand at 2888.337 kb on + strand at 2888.337 kb on + strand at 2888.337 kb on + strand at 2888.518 kb on - strand, within RPSI07_RS21055 at 2888.541 kb on + strand, within RPSI07_RS21055 at 2888.541 kb on + strand, within RPSI07_RS21055 at 2888.541 kb on + strand, within RPSI07_RS21055 at 2888.541 kb on + strand, within RPSI07_RS21055 at 2888.541 kb on + strand, within RPSI07_RS21055 at 2888.542 kb on - strand, within RPSI07_RS21055 at 2888.542 kb on - strand, within RPSI07_RS21055 at 2888.542 kb on - strand, within RPSI07_RS21055 at 2888.683 kb on - strand, within RPSI07_RS21055 at 2888.683 kb on - strand, within RPSI07_RS21055 at 2888.826 kb on + strand, within RPSI07_RS21055 at 2888.901 kb on + strand, within RPSI07_RS21055 at 2889.002 kb on + strand, within RPSI07_RS21055 at 2889.003 kb on + strand, within RPSI07_RS21055 at 2889.003 kb on + strand, within RPSI07_RS21055 at 2889.003 kb on + strand, within RPSI07_RS21055 at 2889.004 kb on - strand, within RPSI07_RS21055 at 2889.004 kb on - strand, within RPSI07_RS21055 at 2889.004 kb on - strand, within RPSI07_RS21055 at 2889.004 kb on - strand, within RPSI07_RS21055 at 2889.004 kb on - strand, within RPSI07_RS21055 at 2889.004 kb on - strand, within RPSI07_RS21055
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_CrystalViolet remove 2,886,657 - RPSI07_RS21040 0.78 +1.6 2,886,923 + -0.8 2,886,924 - -2.4 2,886,924 - -0.5 2,886,928 + -0.1 2,886,929 - -0.5 2,886,929 - +2.9 2,887,216 + -0.8 2,887,217 - +1.6 2,887,244 + -0.3 2,887,245 - +0.0 2,887,245 - +0.8 2,887,245 - -1.0 2,887,268 - -1.5 2,887,268 - -0.1 2,887,432 - RPSI07_RS21045 0.56 +0.4 2,887,432 - RPSI07_RS21045 0.56 +0.4 2,887,519 - RPSI07_RS21045 0.81 -1.7 2,887,519 - RPSI07_RS21045 0.81 -1.1 2,887,633 + +0.2 2,887,633 + -1.9 2,887,634 - -0.7 2,887,636 + -0.2 2,887,636 + +1.6 2,887,636 + -3.9 2,887,637 - -0.2 2,887,637 - -0.5 2,887,637 - +1.7 2,887,637 - -0.7 2,887,637 - -0.0 2,887,860 + RPSI07_RS21050 0.42 +2.0 2,887,860 + RPSI07_RS21050 0.42 +0.9 2,888,012 + RPSI07_RS21050 0.72 +2.0 2,888,012 + RPSI07_RS21050 0.72 +1.2 2,888,012 + RPSI07_RS21050 0.72 +1.2 2,888,012 + RPSI07_RS21050 0.72 +0.5 2,888,012 + RPSI07_RS21050 0.72 +0.5 2,888,013 - RPSI07_RS21050 0.72 +3.2 2,888,013 - RPSI07_RS21050 0.72 +0.3 2,888,016 + RPSI07_RS21050 0.73 +2.6 2,888,016 + RPSI07_RS21050 0.73 +0.8 2,888,016 + RPSI07_RS21050 0.73 +1.2 2,888,016 + RPSI07_RS21050 0.73 +0.5 2,888,016 + RPSI07_RS21050 0.73 +2.4 2,888,016 + RPSI07_RS21050 0.73 +1.3 2,888,017 - RPSI07_RS21050 0.73 +1.1 2,888,017 - RPSI07_RS21050 0.73 +1.0 2,888,017 - RPSI07_RS21050 0.73 +1.7 2,888,017 - RPSI07_RS21050 0.73 +0.2 2,888,017 - RPSI07_RS21050 0.73 +1.6 2,888,069 + RPSI07_RS21050 0.83 +0.5 2,888,070 - RPSI07_RS21050 0.83 +1.6 2,888,185 + +1.6 2,888,185 + -0.2 2,888,185 + +0.9 2,888,186 - +1.1 2,888,337 + -2.4 2,888,337 + -0.7 2,888,337 + -0.4 2,888,518 - RPSI07_RS21055 0.25 -2.6 2,888,541 + RPSI07_RS21055 0.27 +0.2 2,888,541 + RPSI07_RS21055 0.27 -0.6 2,888,541 + RPSI07_RS21055 0.27 -1.0 2,888,541 + RPSI07_RS21055 0.27 +0.4 2,888,541 + RPSI07_RS21055 0.27 -2.1 2,888,542 - RPSI07_RS21055 0.27 +0.6 2,888,542 - RPSI07_RS21055 0.27 -0.9 2,888,542 - RPSI07_RS21055 0.27 +0.4 2,888,683 - RPSI07_RS21055 0.39 +0.8 2,888,683 - RPSI07_RS21055 0.39 -2.1 2,888,826 + RPSI07_RS21055 0.52 +0.2 2,888,901 + RPSI07_RS21055 0.58 -3.1 2,889,002 + RPSI07_RS21055 0.67 -0.9 2,889,003 + RPSI07_RS21055 0.67 +0.6 2,889,003 + RPSI07_RS21055 0.67 -0.2 2,889,003 + RPSI07_RS21055 0.67 -1.4 2,889,004 - RPSI07_RS21055 0.67 -0.3 2,889,004 - RPSI07_RS21055 0.67 +0.1 2,889,004 - RPSI07_RS21055 0.67 +0.7 2,889,004 - RPSI07_RS21055 0.67 -0.7 2,889,004 - RPSI07_RS21055 0.67 -1.0 2,889,004 - RPSI07_RS21055 0.67 -0.9
Or see this region's nucleotide sequence